LOCUS       pSAD-5PSD95-EGFP       18491 bp DNA     circular SYN 06-MAR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 18491)
  AUTHORS   John Naughton
  TITLE     Direct Submission
  JOURNAL   Exported Mar 8, 2024 from SnapGene 7.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..18491
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     primer_bind     113..135
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
                     /note="color: black; sequence: CCCAGTCACGACGTTGTAAAACG"
     primer_bind     127..144
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
                     /note="color: black; sequence: TGTAAAACGACGGCCAGT"
     primer_bind     128..144
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     enhancer        186..565
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        566..769
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     720..740
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
                     /note="color: black; sequence: CGCAAATGGGCGGTAGGCGTG"
     primer_bind     814..833
                     /label=T7
                     /note="T7 promoter, forward primer"
                     /note="color: black; sequence: TAATACGACTCACTATAGGG"
     promoter        814..832
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             969..2321
                     /codon_start=1
                     /label=Rabies N
                     /note="color: #993366"
                     /translation="MDADKIVFKVNNQVVSLKPEIIVDQYEYKYPAIKDLKKPCITLGK
                     APDLNKAYKSVLSGMSAAKLNPDDVCSYLAAAMQFFEGTCPEDWTSYGIVIARKGDKIT
                     PGSLVEIKRTDVEGNWALTGGMELTRDPTVPEHASLVGLLLSLYRLSKISGQNTGNYKT
                     NIADRIEQIFETAPFVKIVEHHTLMTTHKMCANWSTIPNFRFLAGTYDMFFSRIEHLYS
                     AIRVGTVVTAYEDCSGLVSFTGFIKQINLTAREAILYFFHKNFEEEIRRMFEPGQETAV
                     PHSYFIHFRSLGLSGKSPYSSNAVGHVFNLIHFVGCYMGQVRSLNATVIAACAPHEMSV
                     LGGYLGEEFFGKGTFERRFFRDEKELQEYEAAELTKTDVALADDGTVNSDDEDYFSGET
                     RSPEAVYTRIMMNGGRLKRSHIRRYVSVSSNHQARPNSFAEFLNKTYSSDS"
     CDS             2412..3305
                     /codon_start=1
                     /label=Rabies P
                     /note="color: #993366"
                     /translation="MSKIFVNPSAIRAGLADLEMAEETVDLINRNIEDNQAHLQGEPIE
                     VDNLPEDMGRLHLDDGKSPNHGEIAKVGEGKYREDFQMDEGEDPSFLFQSYLENVGVQI
                     VRQMRSGERFLKIWSQTVEEIISYVAVNFPNPPGKSSEDKSTQTTGRELKKETTPTPSQ
                     RESQSSKARMAAQIASGPPALEWSATNEEDDLSVEAEIAHQIAESFSKKYKFPSRSSGI
                     LLYNFEQLKMNLDDIVKEAKNVPGVTRLAHDGSKLPLRCVLGWVALANSKKFQLLVESD
                     KLSKIMQDDLNRYTSC"
     CDS             3364..4002
                     /codon_start=1
                     /label=Rabies M
                     /note="color: #993366"
                     /translation="MKKTGNTTDKMNLLRKIVKNRRDEDTQKSSPASAPLDDDDLWLPP
                     PEYVPLKELTGKKNMRNFCINGRVKVCSPNGYSFRILRHILKSFDEIYSGNHRMIGLVK
                     VVIGLALSGSPVPEGLNWVYKLRRTFIFQWADSRGPLEGEELEYSQEITWDDDTEFVGL
                     QIRVIAKQCHIQGRVWCINMNPRACQLWSDMSLQTQRSEEDKDSSLLLE"
     primer_bind     4203..4234
                     /label=Fragment 2.FOR
                     /note="color: black; sequence: 
                     accccgggaaagccgccaccatggactgtctc"
     primer_bind     complement(4209..4242)
                     /label=Fragment 1.REV
                     /note="color: black; sequence: 
                     actatacagagacagtccatggtggcggctttcc"
     regulatory      4217..4226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             4223..7168
                     /codon_start=1
                     /label=5PSD95-EGFP
                     /note="color: #993366"
                     /translation="MDCLCIVTTRRAEQKLISEEDLGRAKKYRYQDEDTPPLEHSPAHL
                     PNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHI
                     GDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRHY
                     VMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRL
                     QIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYS
                     QHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLG
                     FNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAG
                     QTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKT
                     KDCGFLSQALSFRFGDVLHVIDVGDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSR
                     LKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFG
                     SCVPHTTRPKREYEIDGQDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREV
                     AEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRAT
                     KLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERLPRMVSKGEELFTG
                     VVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQ
                     CFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGI
                     DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTP
                     IGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             4223..4226
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 2 segments:
                        1: 4223 .. 4225 / #05fd14
                        2: 4226 .. 4226 / #05fd14 / 1a"
                     /translation="M"
     CDS             4259..4288
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /note="color: #cc99b2"
                     /translation="EQKLISEEDL"
     primer_bind     5043..5062
                     /label=PSD95 For
                     /note="color: black; sequence: cagacatcacaacctcgtat; added:
                     2019-04-01"
     primer_bind     complement(5644..5663)
                     /label=PSD95 Rev2
                     /note="color: black; sequence: cgtcaatgacatgaagcaca; added:
                     2019-04-01"
     primer_bind     6352..6371
                     /label=SynPhRFP For3
                     /note="color: black; sequence: cgacagctttgaagagatct; added:
                     2019-04-05"
     primer_bind     6386..6405
                     /label=PSD95 For2
                     /note="color: black; sequence: cgtgtcattgaagacctctc; added:
                     2019-04-01"
     CDS             6449..7168
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 6449 .. 6451 / #05fd14
                        2: 6452 .. 6454 / #05fd14 / 1a
                        3: 6455 .. 7168 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6494..6515)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
                     /note="color: black; sequence: CGTCGCCGTCCAGCTCGACCAG"
     primer_bind     complement(6755..6774)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
                     /note="color: black; sequence: GTCTTGTAGTTGCCGTCGTC"
     primer_bind     7102..7123
                     /label=EGFP-C
                     /note="EGFP, forward primer"
                     /note="color: black; sequence: CATGGTCCTGCTGGAGTTCGTG"
     primer_bind     complement(7143..7188)
                     /label=Fragment 2.REV
                     /note="color: black; sequence: 
                     gttgaaaggacgttaattaattacttgtacagctcgtccatgccga"
     primer_bind     7145..7157
                     /label=SynPhRFP For
                     /note="color: black; sequence: acttaatggcatggacgagc; added:
                     2019-04-01"
     primer_bind     7149..7180
                     /label=Vector.FOR
                     /note="color: black; sequence: 
                     tggacgagctgtacaagtaattaattaacgtc"
     regulatory      7232..7241
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             7238..8932
                     /codon_start=1
                     /label=SynPhRFP
                     /note="color: #ff0000"
                     /translation="MDVVNQLVAGGQFRVVKEPLGFVKVLQWVFAIFAFATCGSYTGEL
                     RLSVECANKTESALNIEVEFEYPFRLHQVYFDAPSCVKGGTTKIFLVGDYSSSGEFFVT
                     VAVFAFLYSMGALATYIFLQNKYRENNKGPMMDFLATAVFAFMWLVSSSAWAKGLSDVK
                     MATDPENIIKEMPMCRQTGNTCKELRDPVTSGLNTSVVFGFLNLVLWVGNLWFVFKETG
                     WAAPFMRAPPGAPEKQPAPGDAYGDAGYGQGPGGYGPQDSYGPQGGYQPDYGQPASGGG
                     GGYGPQGDYGQQGYGQQGAPTSFSNQMGGGPGIHRPVATMVSKGEELIKENMHMKLYME
                     GTVNNHHFKCTSEGEGKPYEGTQTMRIKVVEGGPLPFAFDILATSFMYGSRTFINHTQG
                     IPDFFKQSFPEGFTWERVTTYEDGGVLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQ
                     KKTLGWEANTEMLYPADGGLEGRTDMALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVY
                     YVDHRLERIKEADKETYVEQHEVAVARYCDLPSKLGHKLNGMDELYK"
     primer_bind     complement(7316..7335)
                     /label=SynPhRFP Rev3
                     /note="color: black; sequence: aagatggcaaagacccactg; added:
                     2019-04-05"
     primer_bind     complement(8139..8158)
                     /label=SynPhRFP Rev
                     /note="color: black; sequence: ctgattggagaaggaggtgg; added:
                     2019-04-01"
     regulatory      8192..8201
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             8198..8929
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /note="color: #ff0000"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     primer_bind     8902..8921
                     /label=SynPhRFP For
                     /note="color: black; sequence: acttaatggcatggacgagc; added:
                     2019-04-01"
     primer_bind     8913..8932
                     /label=Vector.FOR
                     /note="color: black; sequence: 
                     tggacgagctgtacaagtaattaattaacgtc"
     CDS             9037..15420
                     /codon_start=1
                     /label=Rabies L
                     /note="color: #993366"
                     /translation="MLDPGEVYDDPIDPIELEAEPRGTPIVPNILRNSDYNLNSPLIED
                     PARLMLEWLKTGNRPYRMTLTDNCSRSFRVLKDYFKKVDLGSLKVGGMAAQSMISLWLY
                     GAHSESNRSRRCITDLAHFYSKSSPIEKLLNLTLGNRGLRIPPEGVLSCLERVDYDNAF
                     GRYLANTYSSYLFFHVITLYMNALDWDEEKTILALWKDLTSVDIGKDLVKFKDQIWGLL
                     IVTKDFVYSQSSNCLFDRNYTLMLKDLFLSRFNSLMVLLSPPEPRYSDDLISQLCQLYI
                     AGDQVLSMCGNSGYEVIKILEPYVVNSLVQRAEKFRPLIHSLGDFPVFIKDKVSQLEET
                     FGPCARRFFRALDQFDNIHDLVFVFGCYRHWGHPYIDYRKGLSKLYDQVHLKKMIDKSY
                     QECLASDLARRILRWGFDKYSKWYLDSRFLARDHPLTPYIKTQTWPPKHIVDLVGDTWH
                     KLPITQIFEIPESMDPSEILDDKSHSFTRTRLASWLSENRGGPVPSEKVIITALSKPPV
                     NPREFLRSIDLGGLPDEDLIIGLKPKERELKIEGRFFALMSWNLRLYFVITEKLLANYI
                     LPLFDALTMTDNLNKVFKKLIDRVTGQGLLDYSRVTYAFHLDYEKWNNHQRLESTEDVF
                     SVLDQVFGLKRVFSRTHEFFQKAWIYYSDRSDLIGLREDQIYCLDASNGPTCWNGQDGG
                     LEGLRQKGWSLVSLLMIDRESQIRNTRTKILAQGDNQVLCPTYMLSPGLSQEGLLYELE
                     RISRNALSIYRAVEEGASKLGLIIKKEETMCSYDFLIYGKTPLFRGNILVPESKRWARV
                     SCVSNDQIVNLANIMSTVSTNALTVAQHSQSLIKPMRDFLLMSVQAVFHYLLFSPILKG
                     RVYKILSAEGESFLLAMSRIIYLDPSLGGISGMSLGRFHIRQFSDPVSEGLSFWREIWL
                     SSQESWIHALCQEAGNPDLGERTLESFTRLLEDPTTLNIRGGASPTILLKDAIRKALYD
                     EVDKVENSEFREAILLSKTHRDNFILFLISVEPLFPRFLSELFSSSFLGIPESIIGLIQ
                     NSRTIRRQFRKSLSKTLEESFYNSEIHGISRMTQTPQRVGGVWPCSSERADLLREISWG
                     RKVVGTTVPHPSEMLGLLPKSSISCTCGATGGGNPRVSVSVLPSFDQSFFSRGPLKGYL
                     GSSTSMSTQLFHAWEKVTNVHVVKRALSLKESINWFITRDSNLAQALIRNIMSLTGPDF
                     PLEEAPVFKRTGSALHRFKSARYSEGGYSSVCPNLLSHISVSTDTMSDLTQDGKNYDFM
                     FQPLMLYAQTWTSELVQRDTRLRDSTFHWHLRCNRCVRPIDDVTLETSQIFEFPDVSKR
                     ISRMVSGAVPHFQRLPDIRLRPGDFESLSGREKSHHIGSAQGLLYSILVAIHDSGYNDG
                     TIFPVNIYGKVSPRDYLRGLARGVLIGSSICFLTRMTNININRPLELVSGVISYILLRL
                     DNHPSLYIMLREPSLRGEIFSIPQKIPAAYPTTMKEGNRSILCYLQHVLRYEREIITAS
                     PENDWLWIFSDFRSAKMTYLSLITYQSHLLLQRVERNLSKSMRDNLRQLSSLMRQVLGG
                     HGEDTLESDDNIQRLLKDSLRRTRWVDQEVRHAARTMTGDYSPNKKVSRKVGCSEWVCS
                     AQQVAVSTSANPAPVSELDIRALSKRFQNPLISGLRVVQWATGAHYKLKPILDDLNVFP
                     SLCLVVGDGSGGISRAVLNMFPDAKLVFNSLLEVNDLMASGTHPLPPSAIMRGGNDIVS
                     RVIDLDSIWEKPSDLRNLATWKYFQSVQKQVNMSYDLIICDAEVTDIASINRITLLMSD
                     FALSIDGPLYLVFKTYGTMLVNPNYKAIQHLSRAFPSVTGFITQVTSSFSSELYLRFSK
                     RGKFFRDAEYLTSSTLREMSLVLFNCSSPKSEMQRARSLNYQDLVRGFPEEIISNPYNE
                     MIITLIDSDVESFLVHKMVDDLELQRGTLSKVAIIIAIMIVFSNRVFNVSKPLTDPSFY
                     PPSDPKILRHFNICCSTMMYLSTALGDVPSFARLHDLYNRPITYYFRKQVIRGNVYLSW
                     SWSNDTSVFKRVACNSSLSLSSHWIRLIYKIVKTTRLVGSIKDLSREVERHLHRYNRWI
                     TLEDIRSRSSLLDYSCL"
     primer_bind     complement(9281..9300)
                     /label=Rabies L Rev
                     /note="color: black; sequence: gagagaacccaaatctacct; added:
                     2019-04-01"
     CDS             10681..10692
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 10692"
                     /translation="IEGR"
     primer_bind     complement(15691..15709)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
                     /note="color: black; sequence: GCTAGTTATTGCTCAGCGG"
     terminator      15705..15752
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
                     /note="color: #ffffff"
     primer_bind     complement(15775..15791)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(15775..15791)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
                     /note="color: black; sequence: TCTAGAACTAGTGGATC"
     primer_bind     complement(15828..15848)
                     /label=T3
                     /note="T3 promoter, forward primer"
                     /note="color: black; sequence: GCAATTAACCCTCACTAAAGG"
     promoter        complement(15828..15846)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(15867..15883)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(15867..15883)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     complement(15880..15902)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     protein_bind    15891..15907
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(15915..15945)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 15915 .. 15921 / #ffffff / -10
                        2: 15922 .. 15939 / #ffffff
                        3: 15940 .. 15945 / #ffffff / -35"
     protein_bind    15960..15981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(16098..16115)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     rep_origin      complement(16269..16857)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(16349..16368)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     CDS             complement(17028..17888)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 17028 .. 17819 / #ccffcc
                        2: 17820 .. 17888 / #ccffcc / signal sequence
                     Cleavage site after base 17819"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     17651..17670
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     promoter        complement(17889..17993)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     18000..18031
                     /label=Fragment 1.FOR
                     /note="color: black; sequence: 
                     tccccgaaaagtgccacctgggacgcgccctg"
     primer_bind     complement(18008..18039)
                     /label=Vector.REV
                     /note="color: black; sequence: 
                     cgccgctacagggcgcgtcccaggtggcactt"
     rep_origin      18022..18478
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(18109..18128)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="color: black; sequence: AGGGAAGAAAGCGAAAGGAG"
     primer_bind     18319..18340
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="color: black; sequence: GTGGACTCTTGTTCCAAACTGG"
ORIGIN
        1 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
       61 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      121 cgacgttgta aaacgacggc cagtgagcgc gcgataagct tgatatcgaa ttcctgcagc
      181 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      241 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      301 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      361 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      421 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      481 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      541 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      601 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      661 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      721 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
      781 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
      841 agctggctag attaagcgtc tgatgagtcc gtgaggacga aacccggcgt accgggtcac
      901 gcttaacaac cagatcaaag aaaaaacaga cattgtcaat tgcaaagcaa aaatgtaaca
      961 cccctacaat ggatgccgac aagattgtat tcaaagtcaa taatcaggtg gtctctttga
     1021 agcctgagat tatcgtggat caatatgagt acaagtaccc tgccatcaaa gatttgaaaa
     1081 agccctgtat aaccctagga aaggctcccg atttaaataa agcatacaag tcagttttgt
     1141 caggcatgag cgccgccaaa cttaatcctg acgatgtatg ttcctatttg gcagcggcaa
     1201 tgcagttttt tgaggggaca tgtccggaag actggaccag ctatggaatt gtgattgcac
     1261 gaaaaggaga taagatcacc ccaggttctc tggtggagat aaaacgtact gatgtagaag
     1321 ggaattgggc tctgacagga ggcatggaac tgacaagaga ccccactgtc cctgagcatg
     1381 cgtccttagt cggtcttctc ttgagtctgt ataggttgag caaaatatcc gggcaaaaca
     1441 ctggtaacta taagacaaac attgcagaca ggatagagca gatttttgag acagcccctt
     1501 ttgttaaaat cgtggaacac catactctaa tgacaactca caaaatgtgt gctaattgga
     1561 gtactatacc aaacttcaga tttttggccg gaacctatga catgtttttc tcccggattg
     1621 agcatctata ttcagcaatc agagtgggca cagttgtcac tgcttatgaa gactgttcag
     1681 gactggtatc atttactggg ttcataaaac aaatcaatct caccgctaga gaggcaatac
     1741 tatatttctt ccacaagaac tttgaggaag agataagaag aatgtttgag ccagggcagg
     1801 agacagctgt tcctcactct tatttcatcc acttccgttc actaggcttg agtgggaaat
     1861 ctccttattc atcaaatgct gttggtcacg tgttcaatct cattcacttt gtaggatgct
     1921 atatgggtca agtcagatcc ctaaatgcaa cggttattgc tgcatgtgct cctcatgaaa
     1981 tgtctgttct agggggctat ctgggagagg aattcttcgg gaaagggaca tttgaaagaa
     2041 gattcttcag agatgagaaa gaacttcaag aatacgaggc ggctgaactg acaaagactg
     2101 acgtagcact ggcagatgat ggaactgtca actctgacga cgaggactac ttttcaggtg
     2161 aaaccagaag tccggaggct gtttatactc gaatcatgat gaatggaggt cgactaaaga
     2221 gatctcacat acggagatat gtctcagtca gttccaatca tcaagcccgt ccaaactcat
     2281 tcgccgagtt tctaaacaag acatattcga gtgactcata agaagttgaa taacaaaatg
     2341 ccggaaatct acggattgtg tatatccatc atgaaaaaaa ctaacacccc tcctttcgaa
     2401 ccatcccaaa catgagcaag atctttgtca atcctagtgc tattagagcc ggtctggccg
     2461 atcttgagat ggctgaagaa actgttgatc tgatcaatag aaatatcgaa gacaatcagg
     2521 ctcatctcca aggggaaccc atagaggtgg acaatctccc tgaggatatg gggcgacttc
     2581 acctggatga tggaaaatcg cccaaccatg gtgagatagc caaggtggga gaaggcaagt
     2641 atcgagagga ctttcagatg gatgaaggag aggatcctag cttcctgttc cagtcatacc
     2701 tggaaaatgt tggagtccaa atagtcagac aaatgaggtc aggagagaga tttctcaaga
     2761 tatggtcaca gaccgtagaa gagattatat cctatgtcgc ggtcaacttt cccaaccctc
     2821 caggaaagtc ttcagaggat aaatcaaccc agactactgg ccgagagctc aagaaggaga
     2881 caacacccac tccttctcag agagaaagcc aatcatcgaa agccaggatg gcggctcaaa
     2941 ttgcttctgg ccctccagcc cttgaatggt cggctaccaa tgaagaggat gatctatcag
     3001 tggaggctga gatcgctcac cagattgcag aaagtttctc caaaaaatat aagtttccct
     3061 ctcgatcctc agggatactc ttgtataatt ttgagcaatt gaaaatgaac cttgatgata
     3121 tagttaaaga ggcaaaaaat gtaccaggtg tgacccgttt agcccatgac gggtccaaac
     3181 tccccctaag atgtgtactg ggatgggtcg ctttggccaa ctctaagaaa ttccagttgt
     3241 tagtcgaatc cgacaagctg agtaaaatca tgcaagatga cttgaatcgc tatacatctt
     3301 gctaaccgaa cctctcccct cagtccctct agacaataaa atccgagatg tcccaaagtc
     3361 aacatgaaaa aaacaggcaa caccactgat aaaatgaacc tcctacgtaa gatagtgaaa
     3421 aaccgcaggg acgaggacac tcaaaaatcc tctcccgcgt cagcccctct ggatgacgat
     3481 gacttgtggc ttccaccccc tgaatacgtc ccgctgaaag aacttacagg caagaagaac
     3541 atgaggaact tttgtatcaa cggaagggtt aaagtgtgta gcccgaatgg ttactcgttc
     3601 aggatcctgc ggcacattct gaaatcattc gacgagatat attctgggaa tcataggatg
     3661 atcgggttag tcaaagtggt tattggactg gctttgtcag gatctccagt ccctgagggc
     3721 ctgaactggg tatacaaatt gaggagaacc tttatcttcc agtgggctga ttccaggggc
     3781 cctcttgaag gggaggagtt ggaatactct caggagatca cttgggatga tgatactgag
     3841 ttcgtcggat tgcaaataag agtgattgca aaacagtgtc atatccaggg cagagtctgg
     3901 tgtatcaaca tgaacccgag agcatgtcaa ctatggtctg acatgtctct tcagacacaa
     3961 aggtccgaag aggacaaaga ttcctctctg cttctagaat aatcagatta tatcccgcaa
     4021 atttatcact tgtttacctc tggaggagag aacatatggg ctcaactcca acccttggga
     4081 gcaatataac aaaaaacatg ttatggtgcc attaaaccgc tgcatttcat caaagtcaag
     4141 ttgattacct ttacattttg atcctcttgg atgtgaaaaa aactattaac atccctcaaa
     4201 agaccccggg aaagccgcca ccatggactg tctctgtata gtgacaaccc ggcgcgccga
     4261 gcaaaagctg atatctgaag aggacttggg gcgcgccaag aaataccgct accaagatga
     4321 agacacgccc cctctggaac acagcccggc ccacctcccc aaccaggcca attctccccc
     4381 tgtgattgtc aacacggaca ccctagaagc cccaggatat gagttgcagg tgaatggaac
     4441 agagggggag atggagtatg aggagatcac attggaaagg ggtaactcag gtctgggctt
     4501 cagcatcgca ggtggcactg acaacccgca catcggtgac gacccgtcca tttttatcac
     4561 caagatcatt cctggtgggg ctgcagccca ggatggccgc ctcagggtca atgacagcat
     4621 cctgtttgta aatgaagtgg atgttcggga ggtgacccat tcagctgcgg tggaggccct
     4681 caaagaggca ggttccatcg ttcgccacta tgtcatgcgc cggaaacccc cagccgaaaa
     4741 ggtcatggag atcaaactca tcaaagggcc taaaggactt ggcttcagca ttgcgggggg
     4801 cgttgggaac cagcacatcc ctggagataa cagcatctat gtaacgaaga tcatcgaagg
     4861 aggtgctgct cacaaggatg gcaggttgca gattggagac aagatcctgg cggtcaacag
     4921 tgtggggctg gaggacgtta tgcacgagga tgccgtggca gccctgaaga acacatatga
     4981 cgttgtgtac ctaaaggtgg ccaagcccag caatgcctac ctgagtgaca gctatgctcc
     5041 cccagacatc acaacctcgt attctcagca cctggacaat gagatcagtc atagcagcta
     5101 cttgggcact gactacccca cagccatgac ccccacttcc cctcggcgct actcccctgt
     5161 ggccaaggac ctgctggggg aggaagacat tccccgggaa ccaaggcgga tcgtgatcca
     5221 tcggggctcc accggcctgg gcttcaacat cgtgggcggc gaggatggtg aaggcatctt
     5281 catctccttc atccttgctg ggggtccagc cgacctcagt ggggagctac ggaaggggga
     5341 ccagatcctg tcggtcaatg gtgttgacct ccgcaatgcc agtcacgaac aggctgccat
     5401 tgccctgaag aatgcgggtc agacggtcac gatcatcgct cagtataaac cagaagagta
     5461 tagtcgattc gaggccaaga tccatgatct tcgggaacag ctcatgaata gtagcctagg
     5521 ctcagggact gcatccttgc gaagcaaccc caagaggggc ttctacatta gggccctgtt
     5581 tgattacgac aagaccaagg actgcggttt cttgagccag gccctgagct tccgcttcgg
     5641 ggatgtgctt catgtcattg acgttggtga cgaagagtgg tggcaagcac ggcgggtcca
     5701 ctccgacagt gagaccgacg acattggctt cattcccagc aaacggcggg tcgagcgacg
     5761 agagtggtca aggttaaagg ccaaggactg gggctccagc tctggatcac agggtcgaga
     5821 agactcggtt ctgagctatg agacggtgac ccagatggaa gtgcactatg ctcgtcccat
     5881 catcatcctt ggacccacca aagaccgtgc caacgatgat cttctctccg agttccccga
     5941 caagtttgga tcctgtgtcc ctcatacgac acgtcctaag cgggaatatg agatagacgg
     6001 ccaggattac cactttgtct cctcccggga gaaaatggag aaggacatcc aggcacacaa
     6061 gttcattgag gctggccagt acaacagcca cctctatggg accagcgtcc agtctgtgcg
     6121 agaggtagca gagcagggga agcactgcat cctcgatgtc tcggccaatg ccgtgcggcg
     6181 gctgcaggcg gcccacctgc accccatcgc catcttcatc cgtccccgct ccctggagaa
     6241 tgtgctagag atcaataagc ggatcacaga ggagcaagcc cggaaagcct tcgacagagc
     6301 cacgaagctg gagcaggagt tcacagagtg cttctcagcc atcgtagagg gcgacagctt
     6361 tgaagagatc tatcacaaag tgaaacgtgt cattgaagac ctctcaggcc cctacatctg
     6421 ggtcccagcc cgagagagac tcccgcggat ggtgagcaag ggcgaggagc tgttcaccgg
     6481 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     6541 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     6601 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     6661 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     6721 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     6781 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     6841 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     6901 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     6961 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     7021 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     7081 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     7141 tctcggcatg gacgagctgt acaagtaatt aattaacgtc ctttcaacga tccaagtcca
     7201 tgaaaaaaac taacacccct cccgtacggc cgccaccatg gacgtggtga atcagctggt
     7261 ggctgggggt cagttccggg tggtcaagga gccccttggc ttcgtgaagg tgctgcagtg
     7321 ggtctttgcc atcttcgcct ttgctacgtg cggcagctac accggagagc ttcggctgag
     7381 cgtggagtgt gccaacaaga cggagagtgc cctcaacatc gaagtcgaat ttgagtaccc
     7441 attcaggctg caccaagtgt actttgatgc accctcctgc gttaaagggg gcactaccaa
     7501 gatcttccta gttggtgact actcctcctc gggtgaattc tttgtcaccg tggctgtgtt
     7561 tgccttcctc tactccatgg gggccctggc cacctacatc ttcctgcaga acaagtaccg
     7621 agagaacaac aaagggccaa tgatggactt cctggccaca gcagtgttcg ctttcatgtg
     7681 gctagttagc tcatccgcct gggccaaagg cctgtccgat gtgaagatgg ccactgaccc
     7741 agagaacatt atcaaggaga tgcctatgtg ccgccagaca ggaaacacat gcaaggaact
     7801 gagggaccct gtgacttcag gactcaacac ctcggtggtg tttggcttcc tgaacctggt
     7861 gctctgggtt ggcaacctat ggttcgtgtt caaggagaca ggctgggccg ccccattcat
     7921 gcgcgcacct ccaggcgccc cagaaaagca accagctcct ggcgatgcct acggcgatgc
     7981 gggctatggg cagggccccg gaggctatgg gccccaggac tcctacgggc ctcagggtgg
     8041 ttatcaaccc gattacgggc agccagccag cggcggtggc ggtggctacg ggcctcaggg
     8101 cgactatggg cagcaaggct acggccaaca gggtgcgccc acctccttct ccaatcagat
     8161 gggcggaggc cctgggatcc accggccggt cgccaccatg gtgtctaagg gcgaagagct
     8221 gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt
     8281 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     8341 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
     8401 cggcagcaga accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt
     8461 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     8521 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     8581 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccaacac
     8641 cgagatgctg taccccgctg acggcggcct ggaaggcaga accgacatgg ccctgaagct
     8701 cgtgggcggg ggccacctga tctgcaactt caagaccaca tacagatcca agaaacccgc
     8761 taagaacctc aagatgcccg gcgtctacta tgtggaccac agactggaaa gaatcaagga
     8821 ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
     8881 ccctagcaaa ctggggcaca aacttaatgg catggacgag ctgtacaagt aagctagctt
     8941 ataaagtgct gggtcatcta agcttttcag tcgagaaaaa aacattagat cagaagaaca
     9001 actggcaaca cttctcaacc tgagacttac ttcaagatgc tcgatcctgg agaggtctat
     9061 gatgacccta ttgacccaat cgagttagag gctgaaccca gaggaacccc cattgtcccc
     9121 aacatcttga ggaactctga ctacaatctc aactctcctt tgatagaaga tcctgctaga
     9181 ctaatgttag aatggttaaa aacagggaat agaccttatc ggatgactct aacagacaat
     9241 tgctccaggt ctttcagagt tttgaaagat tatttcaaga aggtagattt gggttctctc
     9301 aaggtgggcg gaatggctgc acagtcaatg atttctctct ggttatatgg tgcccactct
     9361 gaatccaaca ggagccggag atgtataaca gacttggccc atttctattc caagtcgtcc
     9421 cccatagaga agctgttgaa tctcacgcta ggaaatagag ggctgagaat ccccccagag
     9481 ggagtgttaa gttgccttga gagggttgat tatgataatg catttggaag gtatcttgcc
     9541 aacacgtatt cctcttactt gttcttccat gtaatcacct tatacatgaa cgccctagac
     9601 tgggatgaag aaaagaccat cctagcatta tggaaagatt taacctcagt ggacatcggg
     9661 aaggacttgg taaagttcaa agaccaaata tggggactgc tgatcgtgac aaaggacttt
     9721 gtttactccc aaagttccaa ttgtcttttt gacagaaact acacacttat gctaaaagat
     9781 cttttcttgt ctcgcttcaa ctccttaatg gtcttgctct ctcccccaga gccccgatac
     9841 tcagatgact tgatatctca actatgccag ctgtacattg ctggggatca agtcttgtct
     9901 atgtgtggaa actccggcta tgaagtcatc aaaatattgg agccatatgt cgtgaatagt
     9961 ttagtccaga gagcagaaaa gtttaggcct ctcattcatt ccttgggaga ctttcctgta
    10021 tttataaaag acaaggtaag tcaacttgaa gagacgttcg gtccctgtgc aagaaggttc
    10081 tttagggctc tggatcaatt cgacaacata catgacttgg tttttgtgtt tggctgttac
    10141 aggcattggg ggcacccata tatagattat cgaaagggtc tgtcaaaact atatgatcag
    10201 gttcacctta aaaaaatgat agataagtcc taccaggagt gcttagcaag cgacctagcc
    10261 aggaggatcc ttagatgggg ttttgataag tactccaagt ggtatctgga ttcaagattc
    10321 ctagcccgag accacccctt gactccttat atcaaaaccc aaacatggcc acccaaacat
    10381 attgtagact tggtggggga tacatggcac aagctcccga tcacgcagat ctttgagatt
    10441 cctgaatcaa tggatccgtc agaaatattg gatgacaaat cacattcttt caccagaacg
    10501 agactagctt cttggctgtc agaaaaccga ggggggcctg ttcctagcga aaaagttatt
    10561 atcacggccc tgtctaagcc gcctgtcaat ccccgagagt ttctgaggtc tatagacctc
    10621 ggaggattgc cagatgaaga cttgataatt ggcctcaagc caaaggaacg ggaattgaag
    10681 attgaaggtc gattctttgc tctaatgtca tggaatctaa gattgtattt tgtcatcact
    10741 gaaaaactct tggccaacta catcttgcca ctttttgacg cgctgactat gacagacaac
    10801 ctgaacaagg tgtttaaaaa gctgatcgac agggtcaccg ggcaagggct tttggactat
    10861 tcaagggtca catatgcatt tcacctggac tatgaaaagt ggaacaacca tcaaagatta
    10921 gagtcaacag aggatgtatt ttctgtccta gatcaagtgt ttggattgaa gagagtgttt
    10981 tctagaacac acgagttttt tcaaaaggcc tggatctatt attcagacag atcagacctc
    11041 atcgggttac gggaggatca aatatactgc ttagatgcgt ccaacggccc aacctgttgg
    11101 aatggccagg atggcgggct agaaggctta cggcagaagg gctggagtct agtcagctta
    11161 ttgatgatag atagagaatc tcaaatcagg aacacaagaa ccaaaatact agctcaagga
    11221 gacaaccagg ttttatgtcc gacatacatg ttgtcgccag ggctatctca agaggggctc
    11281 ctctatgaat tggagagaat atcaaggaat gcactttcga tatacagagc cgtcgaggaa
    11341 ggggcatcta agctagggct gatcatcaag aaagaagaga ccatgtgtag ttatgacttc
    11401 ctcatctatg gaaaaacccc tttgtttaga ggtaacatat tggtgcctga gtccaaaaga
    11461 tgggccagag tctcttgcgt ctctaatgac caaatagtca acctcgccaa tataatgtcg
    11521 acagtgtcca ccaatgcgct aacagtggca caacactctc aatctttgat caaaccgatg
    11581 agggattttc tgctcatgtc agtacaggca gtctttcact acctgctatt tagcccaatc
    11641 ttaaagggaa gagtttacaa gattctgagc gctgaagggg agagctttct cctagccatg
    11701 tcaaggataa tctatctaga tccttctttg ggagggatat ctggaatgtc cctcggaaga
    11761 ttccatatac gacagttctc agaccctgtc tctgaagggt tatccttctg gagagagatc
    11821 tggttaagct cccaagagtc ctggattcac gcgttgtgtc aagaggctgg aaacccagat
    11881 cttggagaga gaacactcga gagcttcact cgccttctag aagatccgac caccttaaat
    11941 atcagaggag gggccagtcc taccattcta ctcaaggatg caatcagaaa ggctttatat
    12001 gacgaggtgg acaaggtgga aaattcagag tttcgagagg caatcctgtt gtccaagacc
    12061 catagagata attttatact cttcttaata tctgttgagc ctctgtttcc tcgatttctc
    12121 agtgagctat tcagttcgtc ttttttggga atccccgagt caatcattgg attgatacaa
    12181 aactcccgaa cgataagaag gcagtttaga aagagtctct caaaaacttt agaagaatcc
    12241 ttctacaact cagagatcca cgggattagt cggatgaccc agacacctca gagggttggg
    12301 ggggtgtggc cttgctcttc agagagggca gatctactta gggagatctc ttggggaaga
    12361 aaagtggtag gcacgacagt tcctcaccct tctgagatgt tgggattact tcccaagtcc
    12421 tctatttctt gcacttgtgg agcaacagga ggaggcaatc ctagagtttc tgtatcagta
    12481 ctcccgtcct ttgatcagtc atttttttca cgaggccccc taaagggata cttgggctcg
    12541 tccacctcta tgtcgaccca gctattccat gcatgggaaa aagtcactaa tgttcatgtg
    12601 gtgaagagag ctctatcgtt aaaagaatct ataaactggt tcattactag agattccaac
    12661 ttggctcaag ctctaattag gaacattatg tctctgacag gccctgattt ccctctagag
    12721 gaggcccctg tcttcaaaag gacggggtca gccttgcata ggttcaagtc tgccagatac
    12781 agcgaaggag ggtattcttc tgtctgcccg aacctcctct ctcatatttc tgttagtaca
    12841 gacaccatgt ctgatttgac ccaagacggg aagaactacg atttcatgtt ccagccattg
    12901 atgctttatg cacagacatg gacatcagag ctggtacaga gagacacaag gctaagagac
    12961 tctacgtttc attggcacct ccgatgcaac aggtgtgtga gacccattga cgacgtgacc
    13021 ctggagacct ctcagatctt cgagtttccg gatgtgtcga aaagaatatc cagaatggtt
    13081 tctggggctg tgcctcactt ccagaggctt cccgatatcc gtctgagacc aggagatttt
    13141 gaatctctaa gcggtagaga aaagtctcac catatcggat cagctcaggg gctcttatac
    13201 tcaatcttag tggcaattca cgactcagga tacaatgatg gaaccatctt ccctgtcaac
    13261 atatacggca aggtttcccc tagagactat ttgagagggc tcgcaagggg agtattgata
    13321 ggatcctcga tttgcttctt gacaagaatg acaaatatca atattaatag acctcttgaa
    13381 ttggtctcag gggtaatctc atatattctc ctgaggctag ataaccatcc ctccttgtac
    13441 ataatgctca gagaaccgtc tcttagagga gagatatttt ctatccctca gaaaatcccc
    13501 gccgcttatc caaccactat gaaagaaggc aacagatcaa tcttgtgtta tctccaacat
    13561 gtgctacgct atgagcgaga gataatcacg gcgtctccag agaatgactg gctatggatc
    13621 ttttcagact ttagaagtgc caaaatgacg tacctatccc tcattactta ccagtctcat
    13681 cttctactcc agagggttga gagaaaccta tctaagagta tgagagataa cctgcgacaa
    13741 ttgagttctt tgatgaggca ggtgctgggc gggcacggag aagatacctt agagtcagac
    13801 gacaacattc aacgactgct aaaagactct ttacgaagga caagatgggt ggatcaagag
    13861 gtgcgccatg cagctagaac catgactgga gattacagcc ccaacaagaa ggtgtcccgt
    13921 aaggtaggat gttcagaatg ggtctgctct gctcaacagg ttgcagtctc tacctcagca
    13981 aacccggccc ctgtctcgga gcttgacata agggccctct ctaagaggtt ccagaaccct
    14041 ttgatctcgg gcttgagagt ggttcagtgg gcaaccggtg ctcattataa gcttaagcct
    14101 attctagatg atctcaatgt tttcccatct ctctgccttg tagttgggga cgggtcaggg
    14161 gggatatcaa gggcagtcct caacatgttt ccagatgcca agcttgtgtt caacagtctt
    14221 ttagaggtga atgacctgat ggcttccgga acacatccac tgcctccttc agcaatcatg
    14281 aggggaggaa atgatatcgt ctccagagtg atagatcttg actcaatctg ggaaaaaccg
    14341 tccgacttga gaaacttggc aacctggaaa tacttccagt cagtccaaaa gcaggtcaac
    14401 atgtcctatg acctcattat ttgcgatgca gaagttactg acattgcatc tatcaaccgg
    14461 atcaccctgt taatgtccga ttttgcattg tctatagatg gaccactcta tttggtcttc
    14521 aaaacttatg ggactatgct agtaaatcca aactacaagg ctattcaaca cctgtcaaga
    14581 gcgttcccct cggtcacagg gtttatcacc caagtaactt cgtctttttc atctgagctc
    14641 tacctccgat tctccaaacg agggaagttt ttcagagatg ctgagtactt gacctcttcc
    14701 acccttcgag aaatgagcct tgtgttattc aattgtagca gccccaagag tgagatgcag
    14761 agagctcgtt ccttgaacta tcaggatctt gtgagaggat ttcctgaaga aatcatatca
    14821 aatccttaca atgagatgat cataactctg attgacagtg atgtagaatc ttttctagtc
    14881 cacaagatgg ttgatgatct tgagttacag aggggaactc tgtctaaagt ggctatcatt
    14941 atagccatca tgatagtttt ctccaacaga gtcttcaacg tttccaaacc cctaactgac
    15001 ccctcgttct atccaccgtc tgatcccaaa atcctgaggc acttcaacat atgttgcagt
    15061 actatgatgt atctatctac tgctttaggt gacgtcccta gcttcgcaag acttcacgac
    15121 ctgtataaca gacctataac ttattacttc agaaagcaag tcattcgagg gaacgtttat
    15181 ctatcttgga gttggtccaa cgacacctca gtgttcaaaa gggtagcctg taattctagc
    15241 ctgagtctgt catctcactg gatcaggttg atttacaaga tagtgaagac taccagactc
    15301 gttggcagca tcaaggatct atccagagaa gtggaaagac accttcatag gtacaacagg
    15361 tggatcaccc tagaggatat cagatctaga tcatccctac tagactacag ttgcctgtga
    15421 accggatact cctggaagcc tgcccatgct aagactcttg tgtgatgtat cttgaaaaaa
    15481 acaagatcct aaatctgaac ctttggttgt ttgattgttt ttctcatttt tgttgtttat
    15541 ttgttaagcg tgggtcggca tggcatctcc acctcctcgc ggtccgacct gggcatccga
    15601 aggaggacgc acgtccactc ggatggctaa gggagggcgg ggatccggct gctaacaaag
    15661 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
    15721 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatcca
    15781 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
    15841 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    15901 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    15961 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    16021 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    16081 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    16141 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    16201 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    16261 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    16321 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    16381 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    16441 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    16501 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    16561 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    16621 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    16681 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
    16741 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    16801 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    16861 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    16921 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    16981 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    17041 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    17101 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    17161 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    17221 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    17281 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    17341 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    17401 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    17461 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    17521 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    17581 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    17641 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    17701 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    17761 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    17821 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    17881 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    17941 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    18001 ccccgaaaag tgccacctgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
    18061 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
    18121 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
    18181 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
    18241 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
    18301 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
    18361 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaaat
    18421 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc
    18481 attcgccatt c
//
