LOCUS       1_noro-VP1        6176 bp DNA     circular SYN 11-MAR-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 11, 2024 from SnapGene Viewer 7.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             949..1073
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /note="color: #993366"
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     promoter        complement(1616..1725)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
                     /note="color: #ffffff; direction: LEFT"
     promoter        1855..1946
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             1956..3578
                     /codon_start=1
                     /label=Noro-VP1
                     /note="color: #808080"
                     /translation="MKMASSDANPSDGSAANLVPEVNNEVMALEPVVGAAIAAPVAGQQ
                     NVIDPWIRNNFVQAPGGEFTVSPRNAPGEILWSAPLGPDLNPYLSHLARMYNGYAGGFE
                     VQVILAGNAFTAGKVIFAAVPPNFPTEGLSPSQVTMFPHIVVDVRQLEPVLIPLPDVRN
                     NFYHYNQSNDPTIKLIAMLYTPLRANNAGDDVFTVSCRVLTRPSPDFDFIFLVPPTVES
                     RTKPFSVPVLTVEEMTNSRFPIPLEKLFTGPSSAFVVQPQNGRCTTDGVLLGTTQLSPV
                     NICTFRGDVTHITGSRNYTMNLASQNWNDYDPTEEIPAPLGTPDFVGKIQGVLTQTTRT
                     DGSTRGHKATVYTGSADFAPKLGRVQFETDTDRDFEANQNTKFTPVGVIQDGGTTHRNE
                     PQQWVLPSYSGRNTHNVHLAPAVAPTFPGEQLLFFRSTMPGCSGYPNMDLDCLLPQEWV
                     QYFYQEAAPAQSDVALLRFVNPDTGRVLFECKLHKSGYVTVAHTGQHDLVIPPNGYFRF
                     DSWVNQFYTLAPMGNGTGRRRAV"
     3'UTR           3595..3967
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
                     /note="color: #ffffff"
     rep_origin      complement(4429..5017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(join(5188..5979,5980..6048))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5188 .. 5979 / #ccffcc
                        2: 5980 .. 6048 / #ccffcc / signal sequence
                     Cleavage site after base 5979"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6049..6153)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccgtgac gttaaaacta ttaagccatc caatcgaccg ttagtcgaat caggaccgct
      421 ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa cgtgtggagt ccgctcatta
      481 gagcgtcatg tttagacaag aaagctacat atttaattga tcccgatgat tttattgata
      541 aattgaccct aactccatac acggtattct acaatggcgg ggttttggtc aaaatttccg
      601 gactgcgatt gtacatgctg ttaacggctc cgcccactat taatgaaatt aaaaattcca
      661 attttaaaaa acgcagcaag agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa
      721 atgtcgtgga catgctgaac aacaagatta atatgcctcc gtgtataaaa aaaatattga
      781 acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta caggaagagg tttatactaa
      841 actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg
      901 ctctgacgca tttctacaac cacgactcca agtgtgtggg tgaagtcatg catcttttaa
      961 tcaaatccca agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtggacaagc
     1021 tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa
     1081 aacaattata aatgctaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac
     1141 atttgtagta ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa
     1201 atcattttca aatgattcac agttaatttg cgacaatata attttatttt cacataaact
     1261 agacgccttg tcgtcttctt cttcgtattc cttctctttt tcatttttct cctcataaaa
     1321 attaacatag ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt
     1381 gtcataaata tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct
     1441 gtaatttaca acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa
     1501 atttatataa tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc ctctagaata
     1561 ctgcagtgag cggccgccaa aggcctgttg agctctatga attctagaga tctgtgattg
     1621 taaataaaat gtaatttaca gtatagtatt ttaattaata tacaaatgat ttgataataa
     1681 ttcttattta actataatat attgtgttgg gttgaattaa aggtcccggc atcctcaaat
     1741 gcataatttc atagtccccc ttgttgtaag tgatgcgtat ttctgaatct ttgtaaaata
     1801 gcacacaaga ctccaacgcg tttggcgttt tattttcttg ctcgactcta ggatatcatg
     1861 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
     1921 gttttgtaat aaaaaaacct ataaatatag gatccatgaa gatggcttcc agcgacgcta
     1981 acccttccga cggttctgct gctaacctgg tgcctgaagt gaacaacgaa gtgatggctc
     2041 tggaacccgt cgtgggtgct gctattgctg ctcctgtggc tggacagcag aacgtgatcg
     2101 acccctggat tcgtaacaac ttcgtgcagg ctcccggtgg cgagttcact gttagtccta
     2161 gaaacgctcc cggcgagatc ctttggagtg ctcctttggg tcccgacttg aacccctacc
     2221 tgtctcacct ggctcgtatg tacaacggtt acgctggtgg tttcgaggtg caagtgatcc
     2281 tggctggaaa cgctttcacc gctggcaaag tgatcttcgc tgctgtgcct cctaacttcc
     2341 ctaccgaggg actgtcccct agccaagtga ctatgttccc tcacatcgtg gtggacgtgc
     2401 gtcagttgga acctgtgctg atccctctgc ctgacgtgcg caacaacttc taccactaca
     2461 accagtctaa cgaccccacc atcaagctga tcgctatgct gtacacccct ctgcgtgcta
     2521 acaacgctgg cgacgacgtg ttcactgtgt cctgcagagt gctgacccgt ccttctcctg
     2581 acttcgactt catcttcctg gtgccaccta ccgtcgagtc ccgtaccaag cctttctctg
     2641 tgcctgtcct gaccgtggaa gagatgacca actctcgttt ccccattccg ctggaaaagt
     2701 tgttcaccgg tccatcctcc gctttcgtgg tgcaacctca gaacggtcgt tgcactactg
     2761 acggtgtcct gctgggaact acccagctgt ctcctgtgaa catctgcacc ttcagaggcg
     2821 acgtgaccca catcaccggt tctcgtaact acaccatgaa cctggcttcc cagaactgga
     2881 acgactacga ccctaccgaa gagatccccg ctcctctggg tactccagac ttcgtgggaa
     2941 agatccaggg tgtcctgact cagaccactc gtaccgatgg ttccaccagg ggtcacaagg
     3001 ctaccgtgta cactggttcc gctgacttcg ctcctaagct gggtcgtgtg cagttcgaaa
     3061 ccgacaccga ccgtgacttc gaggctaacc agaacaccaa gttcacccct gtgggtgtca
     3121 tccaggacgg tggtactact caccgtaacg agcctcagca gtgggtcctg ccttcctact
     3181 ctggtcgtaa cacccacaac gtgcacttgg ctccagctgt ggctccaact ttcccaggcg
     3241 agcaactgct gttcttccgt tccaccatgc ctggttgctc tggttacccc aacatggacc
     3301 tggactgcct gctgcctcaa gagtgggtgc agtacttcta tcaagaggct gctcccgctc
     3361 agtctgatgt ggctctgttg cgtttcgtga accccgacac cggtcgtgtc ttgttcgagt
     3421 gcaagctgca caagtccggt tacgtgaccg tggctcacac tggtcaacac gacctggtca
     3481 tccctccaaa cggttacttc cgtttcgact cctgggtcaa ccagttctac accctggctc
     3541 ctatgggcaa cggaactggt agacgtcgtg ctgtgtaata aagcttatta ataaaacacg
     3601 atacattgtt attagtacat ttattaagcg ctagattctg tgcgttgttg atttacagac
     3661 aattgttgta cgtattttaa taattcatta aatttataat ctttagggtg gtatgttaga
     3721 gcgaaaatca aatgattttc agcgtcttta tatctgaatt taaatattaa atcctcaata
     3781 gatttgtaaa ataggtttcg attagtttca aacaagggtt gtttttccga accgatggct
     3841 ggactatcta atggattttc gctcaacgcc acaaaacttg ccaaatcttg tagcagcaat
     3901 ctagctttgt cgatattcgt ttgtgttttg ttttgtaata aaggttcgac gtcgttcaaa
     3961 atattatgcg cttttgtatt tctttcatca ctgtcgttag tgtacaattg actcgacgta
     4021 aacacgttaa ataacgcttg gacatattta acatcgggcg tgttagcttt attaggccga
     4081 ttatcgtcgt cgtcccaacc ctcgtcgtta gaagttgctt ccgaagacga ttttgccata
     4141 gccacacgac gcctattaat tgtgtcggct aacacgtccg cgatcaaatt tgtagttgag
     4201 ctttttgggc ctcatgggcc ttccgctcac tgcccgcttt ccagtcggga aacctgtcgt
     4261 gccagctgca ttaacatggt catagctgtt tccttgcgta ttgggcgctc tccgcttcct
     4321 cgctcactga ctcgctgcgc tcggtcgttc gggtaaagcc tggggtgcct aatgagcaaa
     4381 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4441 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4501 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4561 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4621 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4681 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4741 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4801 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4861 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4921 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4981 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5041 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5101 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5161 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     5221 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     5281 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag aaccacgctc
     5341 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     5401 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     5461 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     5521 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5581 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5641 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5701 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5761 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5821 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     5881 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     5941 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6001 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6061 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6121 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
