LOCUS       M13cp-dg1_hp            6864 bp ds-DNA     circular     10-MAR-2024
DEFINITION  .
SOURCE      Cloning vector M13mp18
  ORGANISM  Cloning vector M13mp18 other sequences; artificial sequences;
            vectors.
COMMENT     Imported using the Genbank importer. File name: m13mp18gbk.gb.txt
            Cloning vector M13mp18, complete sequence. Corrects errors in
            M13mp18 GenBank entry X02513 and NEB sequence file dated 18 Apr
            1994 based on 2002 sequencing of complete M13mp18 phage genome at
            NEB (Stewart, F.J., unpublished).
FEATURES             Location/Qualifiers
     CDS             31..252
                     /label="VIII CDS"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    230..277
                     /label="pIII promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    238..243
                     /label=-35
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    261..266
                     /label=-10
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             308..1582
                     /label="III CDS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1445..1493
                     /label="pVI promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1447..1452
                     /label=-35
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1570..1576
                     /label="pVI RBS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             1585..1923
                     /label="VI CDS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1815..1861
                     /label="pI promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1911..1916
                     /label="pI RBS"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             1925..3205
                     /label="IV CDS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    3226..4081
                     /label="p15a"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(4179..4838)
                     /label="ChlR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(4179..4838)
                     /label="Translation 4838-4179"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"
     misc_feature    5113..5161
                     /label="pII promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    5121..5126
                     /label=-35
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    5144..5149
                     /label=-10
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    5156..5171
                     /label="inhibited by pV"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    5180..5189
                     /label="pII RBS"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             5193..6425
                     /label="II CDS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             6090..6425
                     /label="X CDS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     gene            6090..6425
                     /label="X gene"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    6380..6429
                     /label="pV promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    6422..6432
                     /label="pV RBS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             6437..6700
                     /label="V CDS"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             6702..6803
                     /label="VII CDS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             6800..34
                     /label="IX CDS"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct caaagcctct
       61 gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga cgatcccgca
      121 aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta tgcgtgggcg
      181 atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa attcacctcg
      241 aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt ttttggagat
      301 tttcaacgtg aaaaaattat tattcgcaat tcctttagtt gttcctttct attctcactc
      361 cgctgaaact gttgaaagtt gtttagcaaa atcccataca gaaaattcat ttactaacgt
      421 ctggaaagac gacaaaactt tagatcgtta cgctaactat gagggctgtc tgtggaatgc
      481 tacaggcgtt gtagtttgta ctggtgacga aactcagtgt tacggtacat gggttcctat
      541 tgggcttgct atccctgaaa atgagggtgg tggctctgag ggtggcggtt ctgagggtgg
      601 cggttctgag ggtggcggta ctaaacctcc tgagtacggt gatacaccta ttccgggcta
      661 tacttatatc aaccctctcg acggcactta tccgcctggt actgagcaaa accccgctaa
      721 tcctaatcct tctcttgagg agtctcagcc tcttaatact ttcatgtttc agaataatag
      781 gttccgaaat aggcaggggg cattaactgt ttatacgggc actgttactc aaggcactga
      841 ccccgttaaa acttattacc agtacactcc tgtatcatca aaagccatgt atgacgctta
      901 ctggaacggt aaattcagag actgcgcttt ccattctggc tttaatgagg atttatttgt
      961 ttgtgaatat caaggccaat cgtctgacct gcctcaacct cctgtcaatg ctggcggcgg
     1021 ctctggtggt ggttctggtg gcggctctga gggtggtggc tctgagggtg gcggttctga
     1081 gggtggcggc tctgagggag gcggttccgg tggtggctct ggttccggtg attttgatta
     1141 tgaaaagatg gcaaacgcta ataagggggc tatgaccgaa aatgccgatg aaaacgcgct
     1201 acagtctgac gctaaaggca aacttgattc tgtcgctact gattacggtg ctgctatcga
     1261 tggtttcatt ggtgacgttt ccggccttgc taatggtaat ggtgctactg gtgattttgc
     1321 tggctctaat tcccaaatgg ctcaagtcgg tgacggtgat aattcacctt taatgaataa
     1381 tttccgtcaa tatttacctt ccctccctca atcggttgaa tgtcgccctt ttgtctttgg
     1441 cgctggtaaa ccatatgaat tttctattga ttgtgacaaa ataaacttat tccgtggtgt
     1501 ctttgcgttt cttttatatg ttgccacctt tatgtatgta ttttctacgt ttgctaacat
     1561 actgcgtaat aaggagtctt aatcatgcca gttcttttgg gtattccgtt attattgcgt
     1621 ttcctcggtt tccttctggt aactttgttc ggctatctgc ttacttttct taaaaagggc
     1681 ttcggtaaga tagctattgc tatttcattg tttcttgctc ttattattgg gcttaactca
     1741 attcttgtgg gttatctctc tgatattagc gctcaattac cctctgactt tgttcagggt
     1801 gttcagttaa ttctcccgtc taatgcgctt ccctgttttt atgttattct ctctgtaaag
     1861 gctgctattt tcatttttga cgttaaacaa aaaatcgttt cttatttgga ttgggataaa
     1921 taatatgaaa ttgttaaatg taattaattt tgttttcttg atgtttgttt catcatcttc
     1981 ttttgctcag gtaattgaaa tgaataattc gcctctgcgc gattttgtaa cttggtattc
     2041 aaagcaatca ggcgaatccg ttattgtttc tcccgatgta aaaggtactg ttactgtata
     2101 ttcatctgac gttaaacctg aaaatctacg caatttcttt atttctgttt tacgtgcaaa
     2161 taattttgat atggtaggtt ctaacccttc cattattcag aagtataatc caaacaatca
     2221 ggattatatt gatgaattgc catcatctga taatcaggaa tatgatgata attccgctcc
     2281 ttctggtggt ttctttgttc cgcaaaatga taatgttact caaactttta aaattaataa
     2341 cgttcgggca aaggatttaa tacgagttgt cgaattgttt gtaaagtcta atacttctaa
     2401 atcctcaaat gtattatcta ttgacggctc taatctatta gttgttagtg ctcctaaaga
     2461 tattttagat aaccttcctc aattcctttc aactgttgat ttgccaactg accagatatt
     2521 gattgagggt ttgatatttg aggttcagca aggtgatgct ttagattttt catttgctgc
     2581 tggctctcag cgtggcactg ttgcaggcgg tgttaatact gaccgcctca cctctgtttt
     2641 atcttctgct ggtggttcgt tcggtatttt taatggcgat gttttagggc tatcagttcg
     2701 cgcattaaag actaatagcc attcaaaaat attgtctgtg ccacgtattc ttacgctttc
     2761 aggtcagaag ggttctatct ctgttggcca gaatgtccct tttattactg gtcgtgtgac
     2821 tggtgaatct gccaatgtaa ataatccatt tcagacgatt gagcgtcaaa atgtaggtat
     2881 ttccatgagc gtttttcctg ttgcaatggc tggcggtaat attgttctgg atattaccag
     2941 caaggccgat agtttgagtt cttctactca ggcaagtgat gttattacta atcaaagaag
     3001 tattgctaca acggttaatt tgcgtgatgg acagactctt ttactcggtg gcctcactga
     3061 ttataaaaac acttctcagg attctggcgt accgttcctg tctaaaatcc ctttaatcgg
     3121 cctcctgttt agctcccgct ctgattctaa cgaggaaagc acgttatacg tgctcgtcaa
     3181 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ctgcagagaa catggcttca
     3241 tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat
     3301 tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg
     3361 gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg
     3421 agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa
     3481 tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3541 cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     3601 cgctgttatg gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt
     3661 cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc
     3721 ggtaactatc gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc
     3781 actggtaatt gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga
     3841 aaggacaagt tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt
     3901 agctcagaga accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga
     3961 gattacgcgc agaccaaaac gatctcaaga agatcatctt attaaggggt ctgacgctca
     4021 gtggaacgaa aactcacgtt aagggatttt ggtcatgaac aataaaactg tctgctctgc
     4081 aggacgcgtg tccgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca
     4141 ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca
     4201 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     4261 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     4321 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     4381 aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa
     4441 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     4501 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg
     4561 gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga
     4621 aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg
     4681 tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta
     4741 taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat
     4801 atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa
     4861 aatctcgata actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg
     4921 gaacctctta cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg
     4981 gtatcaacag ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt
     5041 tattcggaca cgcgtcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5101 attttaacaa aatattaacg tttacaattt aaatatttgc ttatacaatc ttcctgtttt
     5161 tggggctttt ctgattatca accggggtac atatgattga catgctagtt ttacgattac
     5221 cgttcatcga ttctcttgtt tgctccagac tctcaggcaa tgacctgata gcctttgtag
     5281 atctctcaaa aatagctacc ctctccggca ttaatttatc agctagaacg gttgaatatc
     5341 atattgatgg tgatttgact gtctccggcc tttctcaccc ttttgaatct ttacctacac
     5401 attactcagg cattgcattt aaaatatatg agggttctaa aaatttttat ccttgcgttg
     5461 aaataaaggc ttctcccgca aaagtattac agggtcataa tgtttttggt acaaccgatt
     5521 tagctttatg ctctgaggct ttattgctta attttgctaa ttctttgcct tgcctgtatg
     5581 atttattgga tgttaatgct actactatta gtagaattga tgccaccttt tcagctcgcg
     5641 ccccaaatga aaatatagct aaacaggtta ttgaccattt gcgaaatgta tctaatggtc
     5701 aaactaaatc tactcgttcg cagaattggg aatcaactgt tatatggaat gaaacttcca
     5761 gacaccgtac tttagttgca tatttaaaac atgttgagct acagcattat attcagcaat
     5821 taagctctaa gccatccgca aaaatgacct cttatcaaaa ggagcaatta aaggtactct
     5881 ctaatcctga cctgttggag tttgcttccg gtctggttcg ctttgaagct cgaattaaaa
     5941 cgcgatattt gaagtctttc gggcttcctc ttaatctttt tgatgcaatc cgctttgctt
     6001 ctgactataa tagtcagggt aaagacctga tttttgattt atggtcattc tcgttttctg
     6061 aactgtttaa agcatttgag ggggattcaa tgaatattta tgacgattcc gcagtattgg
     6121 acgctatcca gtctaaacat tttactatta ccccctctgg caaaacttct tttgcaaaag
     6181 cctctcgcta ttttggtttt tatcgtcgtc tggtaaacga gggttatgat agtgttgctc
     6241 ttactatgcc tcgtaattcc ttttggcgtt atgtatctgc attagttgaa tgtggtattc
     6301 ctaaatctca actgatgaat ctttctacct gtaataatgt tgttccgtta gttcgtttta
     6361 ttaacgtaga tttttcttcc caacgtcctg actggtataa tgagccagtt cttaaaatcg
     6421 cataaggtaa ttcacaatga ttaaagttga aattaaacca tctcaagccc aatttactac
     6481 tcgttctggt gtttctcgtc agggcaagcc ttattcactg aatgagcagc tttgttacgt
     6541 tgatttgggt aatgaatatc cggttcttgt caagattact cttgatgaag gtcagccagc
     6601 ctatgcgcct ggtctgtaca ccgttcatct gtcctctttc aaagttggtc agttcggttc
     6661 ccttatgatt gaccgtctgc gcctcgttcc ggctaagtaa catggagcag gtcgcggatt
     6721 tcgacacaat ttatcaggcg atgatacaaa tctccgttgt actttgtttc gcgcttggta
     6781 taatcgctgg gggtcaaaga tgagtgtttt agtgtattct tttgcctctt tcgttttagg
     6841 ttggtgcctt cgtagtggca ttac
//