LOCUS       M13cp-dg2_hp            6644 bp ds-DNA     circular     10-MAR-2024
DEFINITION  .
SOURCE      Cloning vector M13mp18
  ORGANISM  Cloning vector M13mp18 other sequences; artificial sequences;
            vectors.
COMMENT     Imported using the Genbank importer. File name: m13mp18gbk.gb.txt
            Cloning vector M13mp18, complete sequence. Corrects errors in
            M13mp18 GenBank entry X02513 and NEB sequence file dated 18 Apr
            1994 based on 2002 sequencing of complete M13mp18 phage genome at
            NEB (Stewart, F.J., unpublished).
FEATURES             Location/Qualifiers
     CDS             31..252
                     /label="VIII CDS"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    230..277
                     /label="pIII promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    238..243
                     /label=-35
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    261..266
                     /label=-10
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             308..1582
                     /label="III CDS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1445..1493
                     /label="pVI promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1447..1452
                     /label=-35
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1570..1576
                     /label="pVI RBS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             1585..1923
                     /label="VI CDS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1815..1861
                     /label="pI promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1911..1916
                     /label="pI RBS"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             1925..2971
                     /label="I CDS"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             2645..2971
                     /label="XI CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2784..2832
                     /label="pIV promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2937..2944
                     /label="pIV RBS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             2949..4229
                     /label="IV CDS"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    4250..5105
                     /label="p15a"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(5203..5862)
                     /label="ChlR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             complement(5203..5862)
                     /label="Translation 5862-5203"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"
     misc_feature    6137..6185
                     /label="pII promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    6145..6150
                     /label=-35
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    6168..6173
                     /label=-10
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    6180..6195
                     /label="inhibited by pV"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    6204..6213
                     /label="pII RBS"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             6217..6480
                     /label="V CDS"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             6482..6583
                     /label="VII CDS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             6580..34
                     /label="IX CDS"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct caaagcctct
       61 gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga cgatcccgca
      121 aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta tgcgtgggcg
      181 atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa attcacctcg
      241 aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt ttttggagat
      301 tttcaacgtg aaaaaattat tattcgcaat tcctttagtt gttcctttct attctcactc
      361 cgctgaaact gttgaaagtt gtttagcaaa atcccataca gaaaattcat ttactaacgt
      421 ctggaaagac gacaaaactt tagatcgtta cgctaactat gagggctgtc tgtggaatgc
      481 tacaggcgtt gtagtttgta ctggtgacga aactcagtgt tacggtacat gggttcctat
      541 tgggcttgct atccctgaaa atgagggtgg tggctctgag ggtggcggtt ctgagggtgg
      601 cggttctgag ggtggcggta ctaaacctcc tgagtacggt gatacaccta ttccgggcta
      661 tacttatatc aaccctctcg acggcactta tccgcctggt actgagcaaa accccgctaa
      721 tcctaatcct tctcttgagg agtctcagcc tcttaatact ttcatgtttc agaataatag
      781 gttccgaaat aggcaggggg cattaactgt ttatacgggc actgttactc aaggcactga
      841 ccccgttaaa acttattacc agtacactcc tgtatcatca aaagccatgt atgacgctta
      901 ctggaacggt aaattcagag actgcgcttt ccattctggc tttaatgagg atttatttgt
      961 ttgtgaatat caaggccaat cgtctgacct gcctcaacct cctgtcaatg ctggcggcgg
     1021 ctctggtggt ggttctggtg gcggctctga gggtggtggc tctgagggtg gcggttctga
     1081 gggtggcggc tctgagggag gcggttccgg tggtggctct ggttccggtg attttgatta
     1141 tgaaaagatg gcaaacgcta ataagggggc tatgaccgaa aatgccgatg aaaacgcgct
     1201 acagtctgac gctaaaggca aacttgattc tgtcgctact gattacggtg ctgctatcga
     1261 tggtttcatt ggtgacgttt ccggccttgc taatggtaat ggtgctactg gtgattttgc
     1321 tggctctaat tcccaaatgg ctcaagtcgg tgacggtgat aattcacctt taatgaataa
     1381 tttccgtcaa tatttacctt ccctccctca atcggttgaa tgtcgccctt ttgtctttgg
     1441 cgctggtaaa ccatatgaat tttctattga ttgtgacaaa ataaacttat tccgtggtgt
     1501 ctttgcgttt cttttatatg ttgccacctt tatgtatgta ttttctacgt ttgctaacat
     1561 actgcgtaat aaggagtctt aatcatgcca gttcttttgg gtattccgtt attattgcgt
     1621 ttcctcggtt tccttctggt aactttgttc ggctatctgc ttacttttct taaaaagggc
     1681 ttcggtaaga tagctattgc tatttcattg tttcttgctc ttattattgg gcttaactca
     1741 attcttgtgg gttatctctc tgatattagc gctcaattac cctctgactt tgttcagggt
     1801 gttcagttaa ttctcccgtc taatgcgctt ccctgttttt atgttattct ctctgtaaag
     1861 gctgctattt tcatttttga cgttaaacaa aaaatcgttt cttatttgga ttgggataaa
     1921 taatatggct gtttattttg taactggcaa attaggctct ggaaagacgc tcgttagcgt
     1981 tggtaagatt caggataaaa ttgtagctgg gtgcaaaata gcaactaatc ttgatttaag
     2041 gcttcaaaac ctcccgcaag tcgggaggtt cgctaaaacg cctcgcgttc ttagaatacc
     2101 ggataagcct tctatatctg atttgcttgc tattgggcgc ggtaatgatt cctacgatga
     2161 aaataaaaac ggcttgcttg ttctcgatga gtgcggtact tggtttaata cccgttcttg
     2221 gaatgataag gaaagacagc cgattattga ttggtttcta catgctcgta aattaggatg
     2281 ggatattatt tttcttgttc aggacttatc tattgttgat aaacaggcgc gttctgcatt
     2341 agctgaacat gttgtttatt gtcgtcgtct ggacagaatt actttacctt ttgtcggtac
     2401 tttatattct cttattactg gctcgaaaat gcctctgcct aaattacatg ttggcgttgt
     2461 taaatatggc gattctcaat taagccctac tgttgagcgt tggctttata ctggtaagaa
     2521 tttgtataac gcatatgata ctaaacaggc tttttctagt aattatgatt ccggtgttta
     2581 ttcttattta acgccttatt tatcacacgg tcggtatttc aaaccattaa atttaggtca
     2641 gaagatgaaa ttaactaaaa tatatttgaa aaagttttct cgcgttcttt gtcttgcgat
     2701 tggatttgca tcagcattta catatagtta tataacccaa cctaagccgg aggttaaaaa
     2761 ggtagtctct cagacctatg attttgataa attcactatt gactcttctc agcgtcttaa
     2821 tctaagctat cgctatgttt tcaaggattc taagggaaaa ttaattaata gcgacgattt
     2881 acagaagcaa ggttattcac tcacatatat tgatttatgt actgtttcca ttaaaaaagg
     2941 taattcaaat gaaattgtta aatgtaatta attttgtttt cttgatgttt gtttcatcat
     3001 cttcttttgc tcaggtaatt gaaatgaata attcgcctct gcgcgatttt gtaacttggt
     3061 attcaaagca atcaggcgaa tccgttattg tttctcccga tgtaaaaggt actgttactg
     3121 tatattcatc tgacgttaaa cctgaaaatc tacgcaattt ctttatttct gttttacgtg
     3181 caaataattt tgatatggta ggttctaacc cttccattat tcagaagtat aatccaaaca
     3241 atcaggatta tattgatgaa ttgccatcat ctgataatca ggaatatgat gataattccg
     3301 ctccttctgg tggtttcttt gttccgcaaa atgataatgt tactcaaact tttaaaatta
     3361 ataacgttcg ggcaaaggat ttaatacgag ttgtcgaatt gtttgtaaag tctaatactt
     3421 ctaaatcctc aaatgtatta tctattgacg gctctaatct attagttgtt agtgctccta
     3481 aagatatttt agataacctt cctcaattcc tttcaactgt tgatttgcca actgaccaga
     3541 tattgattga gggtttgata tttgaggttc agcaaggtga tgctttagat ttttcatttg
     3601 ctgctggctc tcagcgtggc actgttgcag gcggtgttaa tactgaccgc ctcacctctg
     3661 ttttatcttc tgctggtggt tcgttcggta tttttaatgg cgatgtttta gggctatcag
     3721 ttcgcgcatt aaagactaat agccattcaa aaatattgtc tgtgccacgt attcttacgc
     3781 tttcaggtca gaagggttct atctctgttg gccagaatgt cccttttatt actggtcgtg
     3841 tgactggtga atctgccaat gtaaataatc catttcagac gattgagcgt caaaatgtag
     3901 gtatttccat gagcgttttt cctgttgcaa tggctggcgg taatattgtt ctggatatta
     3961 ccagcaaggc cgatagtttg agttcttcta ctcaggcaag tgatgttatt actaatcaaa
     4021 gaagtattgc tacaacggtt aatttgcgtg atggacagac tcttttactc ggtggcctca
     4081 ctgattataa aaacacttct caggattctg gcgtaccgtt cctgtctaaa atccctttaa
     4141 tcggcctcct gtttagctcc cgctctgatt ctaacgagga aagcacgtta tacgtgctcg
     4201 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcctgcag agaacatggc
     4261 ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag cagaatatgt gatacaggat
     4321 atattccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcgga
     4381 aatggcttac gaacggggcg gagatttcct ggaagatgcc aggaagatac ttaacaggga
     4441 agtgagaggg ccgcggcaaa gccgtttttc cataggctcc gcccccctga caagcatcac
     4501 gaaatctgac gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg
     4561 tttccccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc
     4621 attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc
     4681 agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt
     4741 atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc
     4801 agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa
     4861 ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt
     4921 tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc
     4981 aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaag gggtctgacg
     5041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gaacaataaa actgtctgct
     5101 ctgcaggacg cgtgtccgaa tttctgccat tcatccgctt attatcactt attcaggcgt
     5161 agcaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca
     5221 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac
     5281 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt
     5341 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact
     5401 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg
     5461 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg
     5521 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa
     5581 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat
     5641 acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa
     5701 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg
     5761 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg
     5821 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc
     5881 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa
     5941 gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt
     6001 cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg
     6061 tatttattcg gacacgcgtc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6121 gcgaatttta acaaaatatt aacgtttaca atttaaatat ttgcttatac aatcttcctg
     6181 tttttggggc ttttctgatt atcaaccggg gtacatatga ttaaagttga aattaaacca
     6241 tctcaagccc aatttactac tcgttctggt gtttctcgtc agggcaagcc ttattcactg
     6301 aatgagcagc tttgttacgt tgatttgggt aatgaatatc cggttcttgt caagattact
     6361 cttgatgaag gtcagccagc ctatgcgcct ggtctgtaca ccgttcatct gtcctctttc
     6421 aaagttggtc agttcggttc ccttatgatt gaccgtctgc gcctcgttcc ggctaagtaa
     6481 catggagcag gtcgcggatt tcgacacaat ttatcaggcg atgatacaaa tctccgttgt
     6541 actttgtttc gcgcttggta taatcgctgg gggtcaaaga tgagtgtttt agtgtattct
     6601 tttgcctctt tcgttttagg ttggtgcctt cgtagtggca ttac
//