LOCUS       pSD057C                 3014 bp    DNA     circular     13-MAR-2024
DEFINITION  CP colE1 Rop rrnB1 pro1 SD8 rpoZ-cMyc-SD36 SpecR.
ACCESSION   urn.local...h7-hh19sha
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             complement(1..792)
                     /standard_name="aadA"
     source          join(894..>1481,<1..893)
     primer_bind     complement(70..87)
                     /standard_name="JM546"
     primer_bind     721..739
                     /standard_name="JM382"
     regulatory      793..877
                     /note="Geneious type: promoter"
                     /note="aadA promoter"
     primer_bind     complement(841..892)
                     /standard_name="JM556"
     misc_feature    863..892
                     /note="Geneious type: overhang"
                     /standard_name="Gibson"
     primer_bind     863..897
                     /standard_name="JM555"
     modified_base   878..893
                     /note="USER junction"
     rep_origin      894..1481
     source          <1482..>3014
     CDS             complement(1909..2100)
                     /note="Rop"
     modified_base   2196..2210
                     /note="USER junction"
     regulatory      2211..2255
                     /note="Geneious type: terminator"
                     /note="rrnB1 transcriptional terminator"
     modified_base   2263..2278
                     /note="USER junction"
     modified_base   2263..2278
                     /note="USER Junction"
     primer_bind     2291..2309
                     /standard_name="JM545"
     regulatory      2328..2368
                     /note="Geneious type: promoter"
                     /note="pro1 (Insulated Promoter - based on rrnB1 P1)"
     regulatory      2334..2339
                     /note="Geneious type: -35_signal"
                     /note="-35"
     regulatory      2357..2362
                     /note="Geneious type: -10_signal"
                     /note="-10"
     prim_transcript 2369
                     /note="+1"
     modified_base   2401..2424
                     /note="USER junction"
     misc_feature    2425..2439
                     /note="Geneious type: RBS"
                     /note="SD8 (1.00)"
     CDS             2440..2934
                     /standard_name="rpoZ-cMyc-binder CDS"
     gene            2443..2712
                     /note="rpoZ (RNA Omega Subunit)"
     protein_bind    2722..2751
                     /note="cMyc Tag"
     misc_feature    2722..2751
                     /note="Geneious type: overhang"
                     /standard_name="Gibson"
     primer_bind     complement(2725..2751)
                     /standard_name="JM551"
     primer_bind     2725..2751
                     /standard_name="JM804"
     gene            2752..2931
                     /standard_name="Super"
     primer_bind     complement(2932..2959)
                     /standard_name="JM805"
     misc_feature    2934..2959
                     /note="Geneious type: overhang"
                     /standard_name="Gibson"
     primer_bind     2934..2990
                     /standard_name="JM888"
     primer_bind     2934..2959
                     /standard_name="JM780"
     source          <2935..>2959
     modified_base   2938..2956
                     /note="USER Junction"
     source          <2938..>2959
     source          <2957..>2959
     misc_feature    2958..>2959
                     /note="J23107 promoter"
     regulatory      complement(2960..3001)
                     /note="Geneious type: terminator"
                     /note="tetA/orfL bidirectional terminator"
                     /note="termination of bla transcript is slightly weaker
                     than opposite direction"
     modified_base   3002..3014
                     /note="USER junction"
     misc_feature    3014^3015
                     /note="Geneious type: Editing History Deletion"
                     /standard_name="TTATTTGCCGACTACCTTGGTGATCTCGCCTTTCACGTAGTG
                     GACAAATTCTTCCAACTGATCTGCGCGCGAGGCCAAGCGATCTTCTTCTTGTCCAAGA
                     TAAGCCTGTCTAGCTTCAAGTATGACGGGCTGATACTGGGCCGGCAGGCGCTCCATTG
                     CCCAGTCGGCAGCGACATCCTTCGGCGCGATTTTGCCGGTTACTGCGCTGTACCAAAT
                     GCGGGACAACGTAAGCACTACATTTCGCTCATCGCCAGCCCAGTCGGGCGGCGAGTTC
                     CATAGCGTTAAGGTTTCATTTAGCGCCTCAAATAGATCCTGTTCAGGAACCGGATCAA
                     AGAGTTCCTCCGCCGCTGGACCTACCAAGGCAACGCTATGTTCTCTTGCTTTTGTCAG
                     CAAGATAGCCAGATCAATGTCGATCGTGGCTGGCTCGAAGATACCTGCAAGAATGTCA
                     TTGCGCTGCCATTCTCCAAATTGCAGTTCGCGCTTAGCTGGATAACGCCACGGAATGA
                     TGTCGTCGTGCACAACAATGGTGACTTCTACAGCGCGGAGAATCTCGCTCTCTCCAGG
                     GGAAGCCGAAGTTTCCAAAAGGTCGTTGATCAAAGCTCGCCGCGTTGTTTCATCAAGC
                     CTTACGGTCACCGTAACCAGCAAATCAATATCACTGTGTGGCTTCAGGCCGCCATCCA
                     CTGCGGAGCCGTACAAATGTACGGCCAGCAACGTCGGTTCGAGATGGCGCTCGATGAC
                     GCCAACTACCTCTGATAGTTGAGTCGATACTTCGGCGATCACCGCTTCCCTCATACTC
                     TTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACA
                     TATTTGAATGTATTTAGAAAAATATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGT
                     CGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGG
                     TTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTG
                     ATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGC
                     CCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAATGGCGGGATATAACATGAGCTGT
                     CTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTC
                     GGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTG
                     GGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCC
                     AGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCA
                     GCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGTGCGATT
                     TGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGG
                     AGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAAC
                     ATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATG
                     ATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGA
                     CGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGA
                     TTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACG
                     CCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAAT
                     TCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGC
                     CTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCG
                     TATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATC
                     ATGCCATACCGCGAAAGGTTTTGCACCATAGGCCAAATAGGCCGT"
ORIGIN      
        1 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
       61 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
      121 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
      181 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
      241 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
      301 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
      361 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
      421 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
      481 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
      541 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
      601 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
      661 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
      721 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
      781 cgcttccctc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
      841 catgagcgga tacatatttg aatgtattta gaaaaatagg ccaaataggc cgttgagatc
      901 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
      961 tttgtttgcc ggatcaagag ctaccacctc tttttccgaa ggtaactggc ttcagcagag
     1021 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     1081 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     1141 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     1201 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     1261 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     1321 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     1381 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     1441 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     1501 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     1561 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     1621 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     1681 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     1741 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     1801 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     1861 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     1921 accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag
     1981 cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt
     2041 taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac
     2101 tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga
     2161 gaggatgctc acgatacggg ttactgatga tgaacaagag gacatccggt caaataaaac
     2221 gaaaggctca gtcgaaagac tgggcctttc gttttgctga ggattctaga gcacagctaa
     2281 caccacgtcg tccctatctg ctgccctagg tctatgagtg gttgctggat aactttacgg
     2341 gcatgcataa ggctcggtat ctatattcag ggagaccaca acggtttccc tctacaaata
     2401 attttgttta acttttacta gagtaaggag gaaaaaaaaa tggcacgcgt aactgttcag
     2461 gacgctgtag agaaaattgg taaccgtttt gacctggtac tggtcgccgc gcgtcgcgct
     2521 cgtcagatgc aggtaggcgg aaaggatccg ctggtaccgg aagaaaacga taaaaccact
     2581 gtaatcgcgc tgcgcgaaat cgaagaaggt ctgatcaaca accagatcct cgacgttcgc
     2641 gaacgccagg aacagcaaga gcaggaagcc gctgaattac aagccgttac cgctattgct
     2701 gaaggtcgtc gtgcggccgc ggaacaaaag cttatttctg aagaggactt gttcaatgta
     2761 ctgatggtcc ataaacggag tcacactggc gagctcctgc tcttttgtcc tatttgcggg
     2821 ttcacgtgtc ggcagaaggg caacctcctc cggcatatca atctgcacac gggtgaaaaa
     2881 ctgtttaagt accatctcta caattacgcc tgtcagagaa gagatgcttt gtaatctaga
     2941 aggagatttt caacatggga aatataatga ccctcttgat aacccaagag ggcatttttt
     3001 aatgcccatg gcgt
//
