LOCUS       pSD068F                10774 bp    DNA     circular UNA 13-MAR-2024
DEFINITION  pBR322 pro-hPGK Kozak SD36-eGFP-IRES2-mCherry CarbR PuroR.
ACCESSION   urn.local...hg-hh19shc
VERSION     urn.local...hg-hh19shc
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     primer_bind     16..33
                     /standard_name="L4440"
     protein_bind    150..171
                     /bound_moiety="<i>E. coli</i> catabolite activator
                     protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /standard_name="CAP binding site"
     regulatory      join(186..191,192..209,210..216)
                     /note="Geneious type: promoter"
                     /note="promoter for the <i>E. coli lac</i> operon"
                     /standard_name="lac promoter"
     protein_bind    224..240
                     /bound_moiety="<i>lac</i> repressor encoded by
                     <i>lacI</i>"
                     /note="The <i>lac</i> repressor binds to the <i>lac</i>
                     operator to inhibit transcription in <i>E. coli</i>. This
                     inhibition can be relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /standard_name="lac operator"
     primer_bind     229..251
                     /standard_name="M13/pUC Reverse"
     primer_bind     248..264
                     /standard_name="M13 Reverse"
     primer_bind     248..264
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /standard_name="M13 rev"
     primer_bind     283..303
                     /standard_name="T3"
     regulatory      285..303
                     /note="Geneious type: promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /standard_name="T3 promoter"
     regulatory      331..557
                     /note="Geneious type: promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
                     /standard_name="RSV promoter"
     misc_feature    558..738
                     /note="Geneious type: LTR"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /standard_name="5' LTR (truncated)"
     misc_feature    785..910
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /standard_name="HIV-1 Ψ"
     misc_feature    1403..1636
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /standard_name="RRE"
     regulatory      2040..2540
                     /note="Geneious type: promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
                     /standard_name="hPGK promoter"
     primer_bind     2459..2477
                     /standard_name="hPGK-F"
     regulatory      2567..2575
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (<a
                     href=""https://www.ncbi.nlm.nih.gov/pubmed/3313277""
                     title=""https://www.ncbi.nlm.nih.gov/pubmed/3313277"">Koza
                     k, 1987</a>)"
                     /standard_name="Kozak sequence"
     CDS             2573..2791
                     /standard_name="ORF"
     gene            2582..2761
                     /standard_name="SD36"
     misc_feature    2730
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="G"
     CDS             2792..3508
                     /product="enhanced GFP"
                     /transl_table=1
                     /note="mammalian codon-optimized"
                     /codon_start=1
                     /standard_name="EGFP"
     primer_bind     complement(2834..2855)
                     /standard_name="EGFP-N"
     primer_bind     complement(3095..3114)
                     /standard_name="EXFP-R"
     primer_bind     3442..3463
                     /standard_name="EGFP-C"
     misc_feature    join(3532..4106,4107..4109,4110..4118)
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
                     /standard_name="IRES2"
     primer_bind     complement(3699..3716)
                     /standard_name="IRES reverse"
     primer_bind     3926..3945
                     /standard_name="IRES-F"
     regulatory      4128..4137
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (<a
                     href=""https://www.ncbi.nlm.nih.gov/pubmed/3313277""
                     title=""https://www.ncbi.nlm.nih.gov/pubmed/3313277"">Koza
                     k, 1987</a>)"
                     /standard_name="Kozak sequence"
     CDS             4134..4844
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVM
                     NFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDG
                     ALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQ
                     YERAEGRHSTGGMDELYK*"
                     /product="monomeric derivative of DsRed fluorescent
                     protein (<a
                     href=""http://www.ncbi.nlm.nih.gov/pubmed/15558047""
                     title=""http://www.ncbi.nlm.nih.gov/pubmed/15558047"">Shan
                     er et al., 2004</a>)"
                     /transl_table=1
                     /note="mammalian codon-optimized"
                     /codon_start=1
                     /standard_name="mCherry"
     primer_bind     complement(4279..4297)
                     /standard_name="mCherry-R"
     primer_bind     4547..4566
                     /standard_name="mCherry-F"
     misc_feature    4900..5017
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /standard_name="cPPT/CTS"
     regulatory      5151..6329
                     /note="Geneious type: promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
                     /standard_name="EF-1α promoter"
     intron          5382..6320
                     /note="intron upstream of the start codon of human EF-1α"
                     /standard_name="EF-1α intron A"
     primer_bind     6277..6297
                     /standard_name="EF1a-F"
     CDS             6342..6941
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /product="puromycin <i>N</i>-acetyltransferase"
                     /transl_table=1
                     /gene="<i>pac</i> from <i>Streptomyces alboniger</i>"
                     /note="confers resistance to puromycin"
                     /codon_start=1
                     /standard_name="PuroR"
     primer_bind     complement(6342..6361)
                     /standard_name="Puro-R"
     primer_bind     6838..6858
                     /standard_name="Puro-F"
     misc_feature    6957..7545
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /standard_name="WPRE"
     primer_bind     complement(7010..7030)
                     /standard_name="WPRE-R"
     CDS             complement(7428..7439)
                     /translation="IEGR"
                     /product="Factor Xa recognition and cleavage site"
                     /transl_table=1
                     /codon_start=1
                     /standard_name="Factor Xa site"
     misc_feature    7617..7850
                     /note="Geneious type: LTR"
                     /note="self-inactivating&nbsp;3' long terminal repeat
                     (LTR) from HIV-1"
                     /standard_name="3' LTR (ΔU3)"
     misc_feature    7928..8049
                     /note="Geneious type: polyA_signal"
                     /note="SV40 polyadenylation signal"
                     /standard_name="SV40 poly(A) signal"
     primer_bind     complement(7965..7984)
                     /standard_name="SV40pA-R"
     primer_bind     8019..8038
                     /standard_name="EBV-rev"
     rep_origin      8089..8224
     primer_bind     8151..8170
                     /standard_name="SV40pro-F"
     primer_bind     complement(8244..8263)
                     /standard_name="T7"
     regulatory      complement(8245..8263)
                     /note="Geneious type: promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /standard_name="T7 promoter"
     primer_bind     complement(8273..8290)
                     /standard_name="M13 Forward"
     primer_bind     complement(8273..8289)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /standard_name="M13 fwd"
     primer_bind     complement(8282..8304)
                     /standard_name="M13/pUC Forward"
     rep_origin      8431..8886
     primer_bind     complement(8518..8537)
                     /standard_name="F1ori-R"
     primer_bind     8728..8749
                     /standard_name="F1ori-F"
     regulatory      8912..9016
                     /note="Geneious type: promoter"
                     /gene="<i>bla</i>"
                     /standard_name="AmpR promoter"
     CDS             join(9017..9085,9086..9877)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /product="β-lactamase"
                     /transl_table=1
                     /gene="<i>bla</i>"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /codon_start=1
                     /standard_name="AmpR"
     primer_bind     complement(9235..9254)
                     /standard_name="Amp-R"
     rep_origin      10048..10636
     primer_bind     10537..10556
                     /standard_name="pBR322ori-F"
ORIGIN      
        1 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
       61 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      121 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      181 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      241 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
      301 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
      361 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
      421 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
      481 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
      541 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      601 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      661 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      721 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
      781 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
      841 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
      901 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
      961 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     1021 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     1081 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     1141 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     1201 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     1261 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     1321 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     1381 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     1441 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     1501 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     1561 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     1621 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     1681 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     1741 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     1801 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     1861 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     1921 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     1981 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccct
     2041 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
     2101 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
     2161 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
     2221 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
     2281 gactcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
     2341 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
     2401 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
     2461 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     2521 tcaccgacct ctctccccag ggatcgatac cgtcgactcc ggaatagcca ccatgtccgg
     2581 attcaatgta ctgatggtcc ataaacggag tcacactggc gagctcctgc tcttctgtcc
     2641 gatctgcggg ttcacgtgtc ggcagaaggg caacctcctc cggcatatca atctgcacac
     2701 gggtgaaaaa ctgtttaagt accatctcta caattacgcc tgtcagagaa gagatgcttt
     2761 gggcggcggc ggctccggcg gcggcggctc cgtgagcaag ggcgaggagc tgttcaccgg
     2821 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     2881 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     2941 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     3001 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     3061 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     3121 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3181 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     3241 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     3301 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     3361 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     3421 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     3481 tctcggcatg gacgagctgt acaagtaaat gcatgagtaa ctgaggatcc gcccctctcc
     3541 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     3601 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     3661 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     3721 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     3781 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     3841 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     3901 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     3961 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt
     4021 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     4081 tttcctttga aaaacacgat gataatatgg ccacaaccct ggaattcgcc accatggtga
     4141 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     4201 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     4261 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     4321 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     4381 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     4441 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     4501 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     4561 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     4621 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     4681 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     4741 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     4801 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaaactagt aagcttggcg
     4861 taactagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     4921 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     4981 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     5041 agatccactt tgggctcgag ggggcccggg tgcaaagatg gataaagttt taaacagaga
     5101 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
     5161 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     5221 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     5281 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     5341 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
     5401 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
     5461 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
     5521 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
     5581 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
     5641 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
     5701 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
     5761 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     5821 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     5881 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     5941 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
     6001 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     6061 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     6121 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     6181 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     6241 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     6301 gtttttttct tccatttcag gtgtcgtgac gtacggccac catgaccgag tacaagccca
     6361 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     6421 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     6481 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     6541 cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     6601 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     6661 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     6721 ccgtcggagt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     6781 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     6841 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     6901 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aacgcgttaa gtcgacaatc
     6961 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     7021 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7081 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     7141 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     7201 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     7261 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7321 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     7381 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7441 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7501 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     7561 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7621 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     7681 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7741 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7801 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     7861 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7921 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7981 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8041 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     8101 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     8161 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     8221 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     8281 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     8341 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     8401 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     8461 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     8521 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     8581 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     8641 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     8701 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     8761 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     8821 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     8881 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     8941 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     9001 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     9061 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     9121 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     9181 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     9241 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     9301 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     9361 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     9421 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9481 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     9541 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     9601 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     9661 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     9721 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     9781 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     9841 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     9901 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     9961 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10021 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    10081 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    10141 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    10201 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    10261 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    10321 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    10381 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10441 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    10501 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10561 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10621 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    10681 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    10741 cgcctttgag tgagctgata ccgctcgccg cagc
//
