LOCUS       pOET5-CVB3-VLP        9709 bp DNA     circular SYN 01-APR-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9709)
  AUTHORS   Protein Dynamics
  TITLE     Direct Submission
  JOURNAL   Exported Mar 12, 2024 from SnapGene Viewer 7.1.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             949..1073
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /note="color: #993366"
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     misc_feature    1082..1084
                     /label=PolA tail adition motif
                     /note="color: #ffcc99"
     misc_feature    1107..1112
                     /label=PolyA site
                     /note="color: #ccffff"
     misc_feature    1572..1577
                     /label=NotI
                     /note="color: #ffff99"
     polyA_signal    1578..1785
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    1789..3177
                     /label=3D
                     /note="color: #cc99ff"
     misc_feature    3178..3726
                     /label=3C
                     /note="color: #ff00ff"
     misc_feature    3727..3732
                     /label=Kozak
                     /note="color: #a6acb3"
     promoter        complement(3739..3942)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: LEFT"
     enhancer        3943..4246
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        4382..4473
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    4480..4500
                     /label=L21 Enhancer
                     /note="color: #3366ff"
     misc_feature    4493..4500
                     /label=Kozak
                     /note="color: #00ffff"
     misc_feature    4501..4707
                     /label=VP4
                     /note="color: #993366"
     misc_feature    4708..5496
                     /label=VP2
                     /note="color: #00ffff"
     misc_feature    5497..6210
                     /label=VP3
                     /note="color: #ffff00"
     misc_feature    6211..7062
                     /label=VP1
                     /note="color: #ff00ff"
     misc_feature    7470..7475
                     /label=PolyA site
                     /note="color: #ccffff"
     misc_feature    7508..7510
                     /label=PolA tail addition motif
                     /note="color: #ffcc99"
     rep_origin      complement(7962..8550)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(join(8721..9512,9513..9581))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8721 .. 9512 / #ccffcc
                        2: 9513 .. 9581 / #ccffcc / signal sequence"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9582..9686)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
      181 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
      241 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
      301 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
      361 aggccgtgac gttaaaacta ttaagccatc caatcgaccg ttagtcgaat caggaccgct
      421 ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa cgtgtggagt ccgctcatta
      481 gagcgtcatg tttagacaag aaagctacat atttaattga tcccgatgat tttattgata
      541 aattgaccct aactccatac acggtattct acaatggcgg ggttttggtc aaaatttccg
      601 gactgcgatt gtacatgctg ttaacggctc cgcccactat taatgaaatt aaaaattcca
      661 attttaaaaa acgcagcaag agaaacattt gtatgaaaga atgcgtagaa ggaaagaaaa
      721 atgtcgtgga catgctgaac aacaagatta atatgcctcc gtgtataaaa aaaatattga
      781 acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta caggaagagg tttatactaa
      841 actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga aaaccgatgt ttaatcaagg
      901 ctctgacgca tttctacaac cacgactcca agtgtgtggg tgaagtcatg catcttttaa
      961 tcaaatccca agatgtgtat aaaccaccaa actgccaaaa aatgaaaact gtggacaagc
     1021 tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat ttgtaattat tgaataataa
     1081 aacaattata aatgctaaat ttgtttttta ttaacgatac aaaccaaacg caacaagaac
     1141 atttgtagta ttatctataa ttgaaaacgc gtagttataa tcgctgaggt aatatttaaa
     1201 atcattttca aatgattcac agttaatttg cgacaatata attttatttt cacataaact
     1261 agacgccttg tcgtcttctt cttcgtattc cttctctttt tcatttttct cctcataaaa
     1321 attaacatag ttattatcgt atccatatat gtatctatcg tatagagtaa attttttgtt
     1381 gtcataaata tatatgtctt ttttaatggg gtgtatagta ccgctgcgca tagtttttct
     1441 gtaatttaca acagtgctat tttctggtag ttcttcggag tgtgttgctt taattattaa
     1501 atttatataa tcaatgaatt tgggatcgtc ggttttgtac aatatgttgc ctctagaata
     1561 ctgcagtgag cggccgctcc ccagcatgcc tgctattgtc ttcccaatcc tcccccttgc
     1621 tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa tgcgatgcaa
     1681 tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc aaggaaggca
     1741 cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagttaga aggagtccag
     1801 ccacttgcgg cgcagggtgg agaaagcggg cagggtcagg caagcaccga cgggcacgga
     1861 acggatcttg cggatgaatt cctcgtactc gtgctcgccg ttgtgccagg ccagcaggca
     1921 cagggaacgc acgtggtcct gagtgttctt ggggtccttg gtccaacgga tggactcgtg
     1981 gatgtccttc ataggcatca cggggtgcac caggaagggg tactgctcgt cagcacggaa
     2041 gtagcgcttg aggaaggtca cgttagccca ggtcacttcg ttgaagcagt cgcccttgtc
     2101 agcgggggtc atgatcaggc cgcagtcacg accagcttca gccagcaggg aggcgtcgat
     2161 gggccagggg taggaagcga tcacgtcgtc gccgtaagcg atcatacgga actggtccag
     2221 gtcgataccc ttgtacacct tcagcatcag ggtgcggatg atgatgttgt tgatcatgga
     2281 gttgaagatg gaggttccgg agcaaccgga gggcatgcca ccacgcacga agtagtgctt
     2341 gtcacggtac aggtggtggg agttgcacag gtagtcgatg tagttagttt ccttgtggga
     2401 gtagcccagc ttctcgagca gcagcttcag gcaagcgaac cacacggggg acagggaagc
     2461 gtcgtaaccg gagtagtcga aagccaccag gtgaccgtcc agcatcacag ggatcttgga
     2521 ccagaacagg tcggggtcgc aacccacagc ggagccggtc acgataccgg ggttcaggtg
     2581 gaaggtcttg tacaggtttc cgaaggtctg acgcatagcc acagagtcgt tcagggagga
     2641 agcctcgatc agacgggact tgcccttggc caccttctca gcggaacgca gctcgtcctt
     2701 cacgtaggtg accatgggca ggttcaggcc gtacttgtcc atgcattcct tcagcttggt
     2761 caggtcgcgg gtcttcttgg acaggatgtc acgcttcttg atacccaggg ccacgtaggg
     2821 gtaaccagcg gaggtggtca ggtccagagc ttccaggccc tcggtgccgt acacagcgtc
     2881 ctcgaggcgc atgggctcgg tggagatgtc cagggtagcc agctggccag cgtagtggtc
     2941 cacagcctcg agcatgtact cgtcgacgtg ggtgttcacg ttgccgatgt acttggagaa
     3001 gatagcttcc tcgaagttag cacgcagacg ggggtcgccg ttacgcagca cagcgggttc
     3061 cttgttgccc tcgaacactt ggtggaacac ggaaggttcg agcttggtct tggagggagt
     3121 gttgatgacg gggaaaccag cttccttgga ggactcgatg aactcgatct cgccctgctc
     3181 gtcgttgaag tagtgcttca gcagagcggc ggagaagccc tggtgaccgt tgccgcccac
     3241 gtggataccc agcaccttgc cggtggacat caggacacca ccgcactggc cagcacgggt
     3301 ggggaagttg tacatcagca tgcgcttggt gggggtgcca cccaggttca ggaaaccgta
     3361 ctcagtcact tgacccacag ggatgtacat gttggggaac ttggaggtgt tgatagccag
     3421 cacagcctcg ttgacttcca cctcttcctt agccaagaaa ccacggatgt cacggaactt
     3481 ctcgttgcgg ttcagcttca gcagggtcag ctcgaggttg gtgccgtcct tgtccaccag
     3541 ttccttagcg tccaggacac cgatctcttg gtcgttcatc aggatggtgg gaccgggctt
     3601 agcgtgacgg ggcagcacag cccaacggtc gtagatgccc agcatggtga actcgccgta
     3661 ctcggtcttg acggtggagg agttgcgctt catcatagcc acggcgaact cgaacacggg
     3721 gcccatggtg gcccgcggag ctctgcttat atagacctcc caccgtacac gcctaccgcc
     3781 caattgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
     3841 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
     3901 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
     3961 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     4021 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     4081 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     4141 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     4201 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgccgg catcctcaaa
     4261 tgcataattt catagtcccc ctatacatat gacagtcgac tctctcgagc ttcccgggaa
     4321 aggtacctta aagcttattc gtttggcgtt ttattttctt gctcgactct agagatctga
     4381 tatcatggag ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt
     4441 cgtaacagtt ttgtaataaa aaaacctata aataccggta actcctaaaa aaccgccacc
     4501 atgggagcac aagtcagcac gcaaaagacg ggagcacacg aaacaagatt gaacgcctcg
     4561 ggcaacagca tcatccacta cactaacatc aactactaca aggacgctgc ctccaacagc
     4621 gctaaccgtc aggacttcac tcaggaccct ggcaagttca ccgagcccgt gaaggacatc
     4681 atgatcaagt cactgcctgc tctgaactcc cccaccgtcg aggaatgcgg atacagcgac
     4741 agggccagat ctatcactct gggtaacagc accatcacca ctcaggaatg cgccaacgtg
     4801 gtcgtgggtt acggcgtgtg gccagactac ctgaaggact ctgaggctac tgccgaagac
     4861 cagcccaccc agccagacgt ggctacttgc cgcttctaca ccctggactc agtccagtgg
     4921 cagaagactt ctcctggatg gtggtggaag ctgccagacg ccctgtcaaa cctgggtctg
     4981 ttcggccaga acatgcagta ccactacctg ggacgtaccg gctacactgt ccacgtgcag
     5041 tgcaacgctt ccaagttcca ccagggatgc ctgctggtcg tgtgcgtgcc tgaggctgaa
     5101 atgggttgcg ccactctgga caacactcct tcttctgctg agctgctggg tggcgacact
     5161 gctaaggaat tcgccgacaa gccagtcgct tctggctcaa acaagctggt gcagcgcgtc
     5221 gtgtacaacg ccggaatggg tgtcggcgtg ggaaacctga ccatcttccc tcaccagtgg
     5281 atcaacctgc gcactaacaa ctcagctacc atcgtcatgc catacactaa ctccgtgcct
     5341 atggacaaca tgttccgtca caacaacgtc accctgatgg tcatcccttt cgtccccctg
     5401 gactactgcc ctggttccac cacttacgtg cccatcactg tcactatcgc tccaatgtgc
     5461 gccgagtaca acggcctgag gctggctgga caccagggac tgccaaccat gaacactcct
     5521 ggaagctgcc agttcctgac ttctgacgac ttccagtccc ccagcgccat gccacagtac
     5581 gacgtgaccc ccgagatgag aatcccaggt gaagtcaaga acctgatgga gatcgctgaa
     5641 gtggacagcg tcgtgcccgt ccagaacgtg ggcgagaagg tcaactctat ggaagcctac
     5701 cagatcccag tgaggagcaa cgagggttct ggcactcagg tcttcggctt cccactgcag
     5761 cctggatact cttcagtgtt ctccagaacc ctgctgggcg aaatcctgaa ctactacact
     5821 cactggtctg gatcaatcaa gctgaccttc atgttctgcg gttcagctat ggccaccggc
     5881 aagttcctgc tggcttactc ccctcccgga gctggtgctc ctactaagcg cgtggacgcc
     5941 atgctgggaa cccacgtgat ctgggacgtc ggtctgcagt ccagctgcgt gctgtgcatc
     6001 ccctggatca gccagactca ctaccgtttc gtcgcttctg acgagtacac cgccggaggt
     6061 ttcatcactt gctggtacca gaccaacatc gtcgtgcctg ctgacgccca gtcttcatgc
     6121 tacatcatgt gcttcgtctc agcttgcaac gacttctccg tgaggctgct gaaggacact
     6181 cccttcatca gccagcagaa cttcttccag ggtccagtgg aagacgctat cactgctgct
     6241 atcggaagag tggctgacac tgtgggaact ggacctacca actctgaggc tatccctgct
     6301 ctgactgctg ctgaaactgg ccacacctcc caggtggtgc ctggagacac tatgcagacc
     6361 aggcacgtca agaactacca ctccagaagc gagtctacta tcgaaaactt cctgtgcagg
     6421 agcgcttgcg tgtacttcac cgagtacaag aactctggtg ctaagcgcta cgctgaatgg
     6481 gtcctgaccc ctcgccaagc tgctcagctg aggaggaagc tggagttctt cacttacgtc
     6541 cgcttcgacc tggaactgac cttcgtgatc acctcaactc agcagccatc caccactcag
     6601 aaccaggacg ctcagatcct gactcaccag atcatgtacg tgccacctgg cggaccagtg
     6661 cctgacaagg tcgacagcta cgtgtggcag acctcaacta acccatccgt cttctggacc
     6721 gagggaaacg ctcccccacg catgagcatc cctttcctgt ctatcggtaa cgcctactct
     6781 aacttctacg acggctggtc agaattctcc cgtaacggtg tgtacggcat caacactctg
     6841 aacaacatgg gtaccctgta cgctaggcac gtcaacgccg gaagcactgg tcctatcaag
     6901 tctaccatca gaatctactt caagccaaag cacgtgaagg cttggattcc tcgccctcct
     6961 cgcctgtgcc agtacgagaa ggccaagaac gtcaacttcc agccctcagg agtgaccaca
     7021 acgagacaaa gcattaccac gatgactaac acgggagcct tctaaatagg atccacagtc
     7081 gactctctcg agcttcccgg gaaaggtacc ttaaagctta ttaataaaac acgatacatt
     7141 gttattagta catttattaa gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt
     7201 gtacgtattt taataattca ttaaatttat aatctttagg gtggtatgtt agagcgaaaa
     7261 tcaaatgatt ttcagcgtct ttatatctga atttaaatat taaatcctca atagatttgt
     7321 aaaataggtt tcgattagtt tcaaacaagg gttgtttttc cgaaccgatg gctggactat
     7381 ctaatggatt ttcgctcaac gccacaaaac ttgccaaatc ttgtagcagc aatctagctt
     7441 tgtcgatatt cgtttgtgtt ttgttttgta ataaaggttc gacgtcgttc aaaatattat
     7501 gcgcttttgt atttctttca tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt
     7561 taaataacgc ttggacatat ttaacatcgg gcgtgttagc tttattaggc cgattatcgt
     7621 cgtcgtccca accctcgtcg ttagaagttg cttccgaaga cgattttgcc atagccacac
     7681 gacgcctatt aattgtgtcg gctaacacgt ccgcgatcaa atttgtagtt gagctttttg
     7741 ggcctcatgg gccttccgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     7801 gcattaacat ggtcatagct gtttccttgc gtattgggcg ctctccgctt cctcgctcac
     7861 tgactcgctg cgctcggtcg ttcgggtaaa gcctggggtg cctaatgagc aaaaggccag
     7921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8521 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8581 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8641 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8701 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8761 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8821 gagggcttac catctggccc cagtgctgca atgataccgc gagaaccacg ctcaccggct
     8881 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8941 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9001 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9061 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9121 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9181 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9241 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9301 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9361 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9421 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9481 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9541 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9601 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9661 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
