LOCUS       Exported               13622 bp ds-DNA     circular SYN 11-MAR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13622
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(4..804)
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV
                     "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies,
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     promoter        complement(998..1016)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1131..1235
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1236..2096
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2267..2855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    3251..3385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4138..5508)
                     /codon_start=1
                     /label=rep (pIJ101)
                     /translation="MDPASGVIVAQTAAGTSVVLGLMRCGRIWLCPVCAATIRHKRAEE
                     ITAAVVEWIKRGGTAYLVTFTARHGHTDRLADLMDALQGTRKTPDSPRRPGAYQRLITG
                     GTWAGRRAKDGHRAADREGIRDRIGYVGMIRATEVTVGQINGWHPHIHAIVLVGGRTEG
                     ERSAKQIVATFEPTGAALDEWQGHWRSVWTAALRKVNPAFTPDDRHGVDFKRLETERDA
                     NDLAEYIAKTQDGKAPALELARADLKTATGGNVAPFELLGRIGDLTGGMTEDDAAGVGS
                     LEWNLSRWHEYERATRGRRAIEWTRYLRQMLGLDGGDTEADDLDLLLAADADGGELRAG
                     VAVTEDGWHAVTRRALDLEATRAAEGKDGNEDSAAVGERVREVLALADAADTVVVLTAG
                     EVAEAYADMLAALAQRREEATARRRREQDDDQDDDADDRQERAARHIARLASGPTSH"
     promoter        6604..6905
                     /label=rpsL promoter
                     /note="promoter for the Xylanimonas cellulosilytica
                     ribosomal protein S12 gene"
     misc_feature    6906..12005
                     /label=SCBE3-HF1
     CDS             6906..7592
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     misc_feature    7593..7640
                     /label=Linker
     misc_feature    7641..11741
                     /label=SpCas9-HF1
     misc_feature    11742..11753
                     /label=Linker
     CDS             11754..12005
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     misc_feature    12006..12204
                     /label=fd terminator
     oriT            12368..12477
                     /label=incP origin of transfer
                     /note="incP origin of transfer"
     CDS             12510..12881
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     misc_feature    13037..13234
                     /label=sgRNA cloning cassette
     misc_RNA        13037..13113
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    13114..13152
                     /label=B1006 terminator
     misc_feature    13153..13172
                     /label=PCR adaptor
     misc_feature    13173..13234
                     /label=kasOp*
ORIGIN
        1 tcagccaatc gactggcgag cggcatcgca ttcttcgcat cccgcctctg gcggatgcag
       61 gaagatcaac ggatctcggc ccagttgacc cagggctgtc gccacaatgt cgcgggagcg
      121 gatcaaccga gcaaaggcat gaccgactgg accttccttc tgaaggctct tctccttgag
      181 ccacctgtcc gccaaggcaa agcgctcaca gcagtggtca ttctcgagat aatcgacgcg
      241 taccaacttg ccatcctgaa gaatggtgca gtgtctcggc accccatagg gaacctttgc
      301 catcaactcg gcaagatgca gcgtcgtgtt ggcatcgtgt cccacgccga ggagaagtac
      361 ctgcccatcg agttcatgga cacgggcgac cgggcttgca ggcgagtgag gtggcagggg
      421 caatggatca gagatgatct gctctgcctg tggccccgct gccgcaaagg caaatggatg
      481 ggcgctgcgc tttacatttg gcaggcgcca gaatgtgtca gagacaactc caaggtccgg
      541 tgtaacgggc gacgtggcag gatcgaacgg ctcgtcgtcc agacctgacc acgagggcat
      601 gacgagcgtc cctcccggac ccagcgcagc acgcagggcc tcgatcagtc caagtggccc
      661 atcttcgagg ggccggacgc tacggaagga gctgtggacc agcagcacac cgccgggggt
      721 aaccccaagg ttgagaagct gaccgatgag ctcggctttt cgccattcgt attgcacgac
      781 attgcactcc accgctgatg acatcagtcg atcatagcac gatcaacggc actgttgcaa
      841 atagtcggtg gtgataaact tatcatcccc ttttgctgat ggagctgcac atgaacccat
      901 tcaaaggccg gcattttcag cgtgacatca ttctgtgggc cgtacgctgg tactgcaaat
      961 accggcatca gttaccgtga gctgcaggtc gtcggatcct ttagtgaggg ttaattgcgg
     1021 ccgcgaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     1081 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1141 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1201 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1261 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1321 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1381 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1441 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     1501 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1561 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1621 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1681 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1741 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     1801 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1861 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1921 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1981 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2041 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2101 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2161 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2341 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2401 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2461 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2521 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2581 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2641 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2701 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2761 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2821 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2881 ggttcctggc cttttgctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2941 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3001 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gctgacttcc
     3061 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     3121 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     3181 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
     3241 gcacccgtca gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     3301 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     3361 cattataagc tgcaataaac aagttcccgc acgatcagcg tcccgccgac caggagcagt
     3421 gcggccagca ctgccgctaa cgcctggtcc tggtcccggt cctggtggtg catcagtcct
     3481 ccccgtgatc acttcggcac ccaccgtagt gatcaccccc gacagcggat caaggggttt
     3541 gcgggtcccg gtcggcgccg ggcgggggag gcaggagccg ccgacgctgc ctctgggacg
     3601 ggccggacgg cagggggacc ggcggccggg cgagctgcag ccgggggtcc ggcagggccg
     3661 gagcgggcgg aaccgtgctc tgacctgcgg cccgagtttc gtcacgtgac ggaatggaag
     3721 gctgctgcat ttcgtcacgt gacgtatctc ggcgagcgac tgccgacgcc acggcggaca
     3781 cgatcgcctc gcgctggcgc cgggcctcgt acgcccgctg gcggcaggag cggcggcagt
     3841 agtcccggct ccggccgacg ccggattgct tgatctccga gccgcaccag gcgcagagct
     3901 tcgcgccgtc ggcgtccctg ggggtggtgg tgctcatggc cgacgaccgt acgcggcacg
     3961 tctcgtagcg aggcgagtcg ggcgcgaggt accgcctgca cgaagtgccg gcggggccga
     4021 ccccgggcga gtaatcccag gattactccc gcggcttcga ccccggccgc cgtcgccgcg
     4081 tacgtcaccg acccccgccg tacgtcaccg ggatgacgta cggcgggggg gagcgagtta
     4141 gtgcgaagtg ggcccacttg cgagccgggc gatgtgccgg gcggcccgct cctggcggtc
     4201 gtcggcgtcg tcgtcctggt cgtcgtcctg ctctcgccgt cggcgtgcag ttgcttcctc
     4261 gcggcgctgg gcgagggcgg cgagcatgtc ggcgtacgcc tcggccacct cccccgccgt
     4321 gagcaccacc actgtgtcgg ccgcgtcggc cagcgccagg acctcccgca cccgttcgcc
     4381 cacggccgcc gaatcctcgt tgccgtcctt gccttcggcg gcccgggtcg cctcgaggtc
     4441 gagggcgcgg cgggtgaccg cgtgccatcc gtcctcggtc acggcgaccc cggcccgcag
     4501 ctccccgccg tcggcgtcgg ccgccaggag cagatcgagg tcgtcggcct cggtgtcgcc
     4561 gccgtcgagc ccgagcatct gccgcaggta gcgggtccat tcgatggccc ggcgtccccg
     4621 ggttgcccgc tcgtactcgt gccagcgcga gaggttccac tccagcgagc cgaccccggc
     4681 ggcgtcgtcc tcggtcatgc cgccggtcag gtccccgatc cgtccgagga gttcgaacgg
     4741 ggcgacgttc ccgccggtcg ccgtcttgag gtcggcgcgg gcgagttcga gggcgggcgc
     4801 cttcccgtcc tgggtcttgg cgatgtactc ggcgaggtcg ttggcgtcgc gctcggtctc
     4861 cagccgcttg aagtcgacgc cgtgccggtc gtcgggcgtg aaggcggggt tgaccttgcg
     4921 cagggcggcg gtccacacgg accgccagtg cccctgccac tcgtcgagcg cggcgccggt
     4981 cggctcgaag gtggcgacga tctgcttcgc ggaccgctcc ccctcggtcc ggccgccgac
     5041 caggacgatc gcgtggatgt gcgggtgcca gccgttgatc tgccccacgg tgacttcggt
     5101 cgcgcggatc atgccgacgt acccgatccg gtctcggatg ccctcgcggt cggcggcccg
     5161 gtgcccgtcc ttggcccggc gtccggccca cgtgccgccc gtgatcagtc gctggtaggc
     5221 gcccggccgc cgggggctgt ccggcgtctt ccgggtgccc tggagggcgt ccatgaggtc
     5281 cgcgagccgg tccgtgtgcc catggcgggc cgtgaaggtg accaggtagg cggtcccccc
     5341 gcgcttgatc cactcgacca cggcggcggt gatctcctcg gcccgcttgt gccggatcgt
     5401 ggcggcgcag accgggcaga gccagatccg cccgcaccgc atcaggccca ggaccacgga
     5461 cgttccggcc gccgtctggg cgacgatcac gccggaggca gggtccatca gggcgcggcc
     5521 gcagcccttg cacgcggcgt ccccgctgat ccgccacagc gtccggcggc ggctgtaccg
     5581 ggcggctttc cgcagtcggg cagcgtcgct ccgcgacgtg cttcctactt ccgagaggct
     5641 gtcgcctctc gggctctccc catccacccc gtccggagaa accgcaggtc ggaggggtgc
     5701 gggaaactct gttgtttctt tcccaaggtg ttcgcttttg cctcgggcgg catctcgcgt
     5761 cacacgcgcg atcgcccgct tcgctgccat ccggcagcgg tctgagcagt agatacgcgg
     5821 ccgtttgccc ggtgtgtggg caattgcggt cccgcagtgg cagcggggcc cggcgggccg
     5881 atctggcaat gcctcggcat cgctccgtac tctgggcacg agcaacgttc ctgtctcgcc
     5941 cggctaaggg gcgcgagtct gggagcggac gggtcggagg tgcgaagtcc ggcccgttgc
     6001 tctttggtct ggtgggaatc ctggcaccaa tcgggccaga ggttccctcc gccactcccg
     6061 acgccccttg gggctggtgt gacttggagg gccgaagaga gccccgccgg tgatccggcg
     6121 gggctttgac gtgcggtcag tgcgtgtgtc ggcgagcgat ggccacgagg ccctggaagc
     6181 cgagcggtcc ggcgaagtcg gcccagtcgc aaccgggctc agcgcagtgg gcggaccagc
     6241 caccgccgtt gtgggtcctg gaccaggttc acggtcccct cggtcaggcg tccgtcgaag
     6301 tcggtcatgg tcggtctcct ggtgggtggg ggcggggcgc cagcacgaag tgccggcgcc
     6361 ccgcgggggt tggtcgggtc aggcgccgaa ccggcgggcg gcggcggcga ccaggccgtc
     6421 ggcggcggcc atggcgcggt cgcggtcggt ggtgagggcg gtgcggtcgg cggcggccca
     6481 gtcctgctct cacgcaacgt ctacgtggac gctcagggcg acacgatcga ggtcggggag
     6541 tccgtcgctc cggcgggccg ctggcgcgtc caccgggact gatcaaggcg aatacttcat
     6601 atggccctgc aggcggaagt caggtagaca cgacttccgc tagtccttgc aaggtctgct
     6661 gacgtgaggc ggggcggtcg tttttgaccg ccctgccttc gtcatgtagg ctcgctcgct
     6721 gtgcctggcg tgtcatcaga cgcccaggtc ccggtgccgt gaggcccggg ccatcgagcc
     6781 ggtggtacgt ggctgcggtc cccttgtgag ggctgcgcgc cgtgtgctgt ccggcgcgca
     6841 cagccttgaa tccacccgcg ggggccggcc ggtctccgtg agctcgagta gacgacggag
     6901 acgtaatgtc gtccgagacc ggcccggtgg ccgtggaccc gaccctgcgt cgccgcatcg
     6961 agccgcacga gttcgaggtg ttcttcgacc cgcgggagct gcgcaaggag acctgcctgc
     7021 tgtacgagat caactggggc ggccggcact cgatctggcg ccacacctcg cagaacacca
     7081 acaagcacgt cgaggtgaac ttcatcgaga agttcaccac cgagcggtac ttctgcccga
     7141 acacccgctg ctcgatcacc tggttcctgt cctggtcccc gtgcggcgag tgctcccggg
     7201 cgatcaccga gttcctgtcc cgctacccgc acgtcaccct gttcatctac atcgcccggc
     7261 tgtaccacca cgccgacccg cggaaccggc agggcctgcg cgacctgatc tcctccggcg
     7321 tgaccatcca gatcatgacc gagcaggagt ccggctactg ctggcggaac ttcgtcaact
     7381 actcgccgtc caacgaggcc cactggccgc ggtacccgca cctgtgggtg cgcctgtacg
     7441 tcctggagct gtactgcatc atcctgggcc tgccgccgtg cctgaacatc ctgcgtcgca
     7501 agcagccgca gctgaccttc ttcaccatcg ccctgcagtc ctgccactac cagcgcctgc
     7561 cgccgcacat cctgtgggcg accggcctga agtccggctc ggagaccccg ggcacctcgg
     7621 agtccgccac cccggagtcc gacaagaagt acagcatcgg cctggccatc ggcaccaaca
     7681 gcgtgggctg ggcggtcatc accgacgagt acaaggtccc ctccaagaag ttcaaggtcc
     7741 tgggcaacac cgaccggcac tcgatcaaga agaacctgat cggcgccctg ctcttcgaca
     7801 gcggcgagac cgccgaggcg acccgcctga agcggaccgc gcgccggcgc tacacccggc
     7861 gcaagaaccg catctgctac ctccaggaaa tcttctccaa cgagatggcc aaggtcgacg
     7921 actcgttctt ccaccggctc gaggagagct tcctggtgga ggaggacaag aagcacgagc
     7981 gccacccgat cttcggcaac atcgtcgacg aggtggccta ccacgagaag taccccacca
     8041 tctaccacct ccgcaagaag ctggtcgact cgaccgacaa ggcggacctg cggctcatct
     8101 acctggccct cgcgcacatg atcaagttcc gcggccactt cctcatcgag ggcgacctga
     8161 acccggacaa ctccgacgtc gacaagctct tcatccagct ggtgcagacc tacaaccagc
     8221 tgttcgagga gaaccccatc aacgccagcg gcgtcgacgc caaggcgatc ctctccgcgc
     8281 gcctgagcaa gtcccggcgc ctggagaacc tcatcgccca gctgccgggc gagaagaaga
     8341 acggcctctt cggcaacctg atcgcgctgt cgctcggcct gacccccaac ttcaagagca
     8401 acttcgacct ggccgaggac gcgaagctcc agctgtccaa ggacacctac gacgacgacc
     8461 tggacaacct gctcgcccag atcggcgacc agtacgcgga cctcttcctg gccgcgaaga
     8521 acctctcgga cgccatcctg ctcagcgaca tcctgcgggt caacaccgag atcaccaagg
     8581 ccccgctgtc ggcgagcatg atcaagcggt acgacgagca ccaccaggac ctgaccctgc
     8641 tcaaggccct cgtgcgccag cagctgcccg agaagtacaa ggaaatcttc ttcgaccagt
     8701 ccaagaacgg ctacgccggc tacatcgacg gcggcgcgtc gcaggaggag ttctacaagt
     8761 tcatcaagcc gatcctggag aagatggacg gcaccgagga gctgctcgtc aagctgaacc
     8821 gcgaggacct gctccgcaag cagcggacct tcgacaacgg ctccatcccg caccagatcc
     8881 acctgggcga gctgcacgcc atcctccggc gccaggagga cttctacccc ttcctgaagg
     8941 acaaccgcga gaagatcgag aagatcctga ccttccgcat cccgtactac gtcggccccc
     9001 tggcccgcgg caactcccgg ttcgcgtgga tgacccggaa gtcggaggag accatcaccc
     9061 cgtggaactt cgaggaggtc gtggacaagg gcgcctccgc ccagtcgttc atcgagcgca
     9121 tgaccgcctt cgacaagaac ctcccgaacg agaaggtcct gcccaagcac tccctgctct
     9181 acgagtactt caccgtgtac aacgagctga ccaaggtcaa gtacgtgacc gagggcatgc
     9241 ggaagccggc cttcctgtcg ggcgagcaga agaaggcgat cgtcgacctg ctcttcaaga
     9301 ccaaccgcaa ggtcaccgtg aagcagctga aggaggacta cttcaagaag atcgagtgct
     9361 tcgactccgt cgagatcagc ggcgtggagg accgcttcaa cgcctccctg ggcacctacc
     9421 acgacctgct caagatcatc aaggacaagg acttcctcga caacgaggag aacgaggaca
     9481 tcctggagga catcgtcctc accctgaccc tcttcgagga ccgcgagatg atcgaggagc
     9541 ggctcaagac ctacgcccac ctgttcgacg acaaggtgat gaagcagctg aagcggcgcc
     9601 ggtacaccgg ctggggcgcc ctctcccgga agctgatcaa cggcatccgg gacaagcaga
     9661 gcggcaagac catcctggac ttcctcaagt ccgacggctt cgccaaccgc aacttcatgg
     9721 cgctcatcca cgacgacagc ctgaccttca aggaggacat ccagaaggcc caggtctcgg
     9781 gccagggcga cagcctccac gagcacatcg ccaacctggc gggctccccg gcgatcaaga
     9841 agggcatcct ccagaccgtc aaggtcgtgg acgagctggt caaggtgatg ggccgccaca
     9901 agcccgagaa catcgtgatc gagatggccc gggagaacca gaccacccag aagggccaga
     9961 agaactcgcg cgagcggatg aagcggatcg aggagggcat caaggagctg ggcagccaga
    10021 tcctgaagga gcacccggtc gagaacaccc agctccagaa cgagaagctg tacctctact
    10081 acctccagaa cggccgcgac atgtacgtgg accaggagct ggacatcaac cggctgtccg
    10141 actacgacgt cgaccacatc gtgccgcagt ccttcctgaa ggacgactcg atcgacaaca
    10201 aggtcctgac ccgctcggac aagaaccggg gcaagtccga caacgtgccc tcggaggagg
    10261 tcgtgaagaa gatgaagaac tactggcgcc agctgctcaa cgccaagctc atcacccagc
    10321 gcaagttcga caacctgacc aaggccgagc ggggcggcct gagcgagctg gacaaggcgg
    10381 gcttcatcaa gcgccagctg gtcgagaccc gggcgatcac caagcacgtg gcccagatcc
    10441 tggactcccg gatgaacacc aagtacgacg agaacgacaa gctgatccgc gaggtcaagg
    10501 tgatcaccct caagagcaag ctggtctccg acttccgcaa ggacttccag ttctacaagg
    10561 tccgggagat caacaactac caccacgccc acgacgcgta cctgaacgcc gtcgtgggca
    10621 ccgcgctgat caagaagtac ccgaagctgg agtccgagtt cgtctacggc gactacaagg
    10681 tctacgacgt gcgcaagatg atcgccaaga gcgagcagga gatcggcaag gccaccgcga
    10741 agtacttctt ctactccaac atcatgaact tcttcaagac cgagatcacc ctggccaacg
    10801 gcgagatccg caagcggccc ctgatcgaga ccaacggcga gaccggcgag atcgtctggg
    10861 acaagggccg cgacttcgcc accgtccgga aggtgctgtc gatgccgcag gtcaacatcg
    10921 tgaagaagac cgaggtgcag accggcggct tcagcaagga gtccatcctc cccaagcgca
    10981 acagcgacaa gctgatcgcc cggaagaagg actgggaccc gaagaagtac ggcggcttcg
    11041 acagccccac cgtcgcctac tccgtgctgg tcgtggcgaa ggtcgagaag ggcaagagca
    11101 agaagctgaa gtccgtgaag gagctgctcg gcatcaccat catggagcgc tcctcgttcg
    11161 agaagaaccc gatcgacttc ctggaggcca agggctacaa ggaggtcaag aaggacctca
    11221 tcatcaagct gcccaagtac agcctgttcg agctggagaa cggccgcaag cggatgctcg
    11281 cctccgcggg cgagctgcaa aagggcaacg agctggccct cccgtcgaag tacgtcaact
    11341 tcctgtacct cgcgtcccac tacgagaagc tgaagggctc gcccgaggac aacgagcaga
    11401 agcagctctt cgtggagcag cacaagcact acctggacga gatcatcgag cagatcagcg
    11461 agttcagcaa gcgcgtcatc ctggccgacg cgaacctcga caaggtgctg tccgcctaca
    11521 acaagcaccg cgacaagccg atccgggagc aggcggagaa catcatccac ctgttcaccc
    11581 tcaccaacct gggtgccccg gccgccttca agtacttcga caccaccatc gaccgcaagc
    11641 ggtacacctc caccaaggag gtcctcgacg cgaccctgat ccaccagagc atcaccggcc
    11701 tgtacgagac ccgcatcgac ctgagccagc tgggcggcga ctcgggcggc tccaccaacc
    11761 tgtcggacat catcgagaag gagaccggca agcagctggt gatccaggag tccatcctga
    11821 tgctgccgga ggaagtggag gaagtgatcg gcaacaagcc ggagtcggac atcctggtgc
    11881 acaccgccta cgacgagtcg accgacgaga acgtcatgct gctgacctcg gacgccccgg
    11941 agtacaagcc gtgggccctg gtcatccagg actccaacgg cgagaacaag atcaagatgc
    12001 tgtgagaatt cagatctacg cgttccccgc aaaagcggcc tttgactccc tgcaagcctc
    12061 agcgaccgaa tatatcggtt atgcgtgggc gatggttgtt gtcattgtcg gcgcaactat
    12121 cggtatcaag ctgtttaaga aattcacctc gaaagcaagc tgataaaccg atacaattaa
    12181 aggctccttt tggagccttt ttttaagctt gcatgcctgc aggtcgacgg atcttttccg
    12241 ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg
    12301 cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc
    12361 gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg
    12421 tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccggcta
    12481 ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca accaggaagg
    12541 gcagcccacc tatcaaggtg tactgccttc cagacgaacg aagagcgatt gaggaaaagg
    12601 cggcggcggc cggcatgagc ctgtcggcct acctgctggc cgtcggccag ggctacaaaa
    12661 tcacgggcgt cgtggactat gagcacgtcc gcgagctggc ccgcatcaat ggcgacctgg
    12721 gccgcctggg cggcctgctg aaactctggc tcaccgacga cccgcgcacg gcgcggttcg
    12781 gtgatgccac gatcctcgcc ctgctggcga agatcgaaga gaagcaggac gagcttggca
    12841 aggtcatgat gggcgtggtc cgcccgaggg cagagccatg acttttttag ccgctaaaac
    12901 ggccgggggg tgcgcgtgat tgccaagcac gtccccatgc gctccatcaa gaagagcgac
    12961 ttcgcggagc tggtgaagta catcaccgac gagcaaggca agaccgatcc ccggggacct
    13021 gcaggtcgac tctagagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
    13081 atcaacttga aaaagtggca ccgagtcggt gctaaaaaaa aaccccgccc ctgacagggc
    13141 ggggtttttt ttgtagcctc aggagttgta gctgttcaca ttcgaacggt ctctgctttg
    13201 acaacatgct gtgcggtgtt gtaaagtcgt ggccgctagc tatttacccg caggacatat
    13261 ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc
    13321 aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt
    13381 tcaggctttt tcatatctca ttgcccccgg acgagcgtct gctccgccat tcgccgtccg
    13441 ccgtgccaat cggatcagcc gtccaaatgc gggattttcg ttagtcggag gccaaacggc
    13501 attgagcgtc agcatatcat cagcgagctg aagaaagaca atccccgatc cgctccacgt
    13561 gttgccccag caatcagcgc gaccttgccc ctccaacgtc atctcgttct ccgctcatga
    13621 gc
//
