LOCUS       Exported               13580 bp ds-DNA     circular SYN 11-MAR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Mar 12, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13580
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(4..804)
                     /codon_start=1
                     /gene="aac(3)IV"
                     /product="aminoglycoside 3-N-acetyltransferase type IV
                     "
                     /label=ApmR
                     /note="confers resistance to apramycin (Paget and Davies,
                     1996)"
                     /translation="MSSAVECNVVQYEWRKAELIGQLLNLGVTPGGVLLVHSSFRSVRP
                     LEDGPLGLIEALRAALGPGGTLVMPSWSGLDDEPFDPATSPVTPDLGVVSDTFWRLPNV
                     KRSAHPFAFAAAGPQAEQIISDPLPLPPHSPASPVARVHELDGQVLLLGVGHDANTTLH
                     LAELMAKVPYGVPRHCTILQDGKLVRVDYLENDHCCERFALADRWLKEKSLQKEGPVGH
                     AFARLIRSRDIVATALGQLGRDPLIFLHPPEAGCEECDAARQSIG"
     promoter        complement(998..1016)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1131..1235
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1236..2096
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2267..2855
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    3251..3385
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4138..5508)
                     /codon_start=1
                     /label=rep (pIJ101)
                     /translation="MDPASGVIVAQTAAGTSVVLGLMRCGRIWLCPVCAATIRHKRAEE
                     ITAAVVEWIKRGGTAYLVTFTARHGHTDRLADLMDALQGTRKTPDSPRRPGAYQRLITG
                     GTWAGRRAKDGHRAADREGIRDRIGYVGMIRATEVTVGQINGWHPHIHAIVLVGGRTEG
                     ERSAKQIVATFEPTGAALDEWQGHWRSVWTAALRKVNPAFTPDDRHGVDFKRLETERDA
                     NDLAEYIAKTQDGKAPALELARADLKTATGGNVAPFELLGRIGDLTGGMTEDDAAGVGS
                     LEWNLSRWHEYERATRGRRAIEWTRYLRQMLGLDGGDTEADDLDLLLAADADGGELRAG
                     VAVTEDGWHAVTRRALDLEATRAAEGKDGNEDSAAVGERVREVLALADAADTVVVLTAG
                     EVAEAYADMLAALAQRREEATARRRREQDDDQDDDADDRQERAARHIARLASGPTSH"
     promoter        6604..6905
                     /label=rpsL promoter
                     /note="promoter for the Xylanimonas cellulosilytica
                     ribosomal protein S12 gene"
     misc_feature    6906..11963
                     /label=eSCBE3-NG-Hypa
     misc_feature    6906..7502
                     /label=hAPOBEC3A (Y130F)
     misc_feature    7503..7598
                     /label=Linker
     misc_feature    7599..11699
                     /label=SpCas9-NG-Hypa
     misc_feature    11700..11711
                     /label=Linker
     CDS             11712..11963
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     misc_feature    11964..12162
                     /label=fd terminator
     oriT            12326..12435
                     /label=incP origin of transfer
                     /note="incP origin of transfer"
     CDS             12468..12839
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     misc_feature    12995..13192
                     /label=sgRNA cloning cassette
     misc_RNA        12995..13071
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    13072..13110
                     /label=B1006 terminator
     misc_feature    13111..13130
                     /label=PCR adaptor
     misc_feature    13131..13192
                     /label=kasOp*
ORIGIN
        1 tcagccaatc gactggcgag cggcatcgca ttcttcgcat cccgcctctg gcggatgcag
       61 gaagatcaac ggatctcggc ccagttgacc cagggctgtc gccacaatgt cgcgggagcg
      121 gatcaaccga gcaaaggcat gaccgactgg accttccttc tgaaggctct tctccttgag
      181 ccacctgtcc gccaaggcaa agcgctcaca gcagtggtca ttctcgagat aatcgacgcg
      241 taccaacttg ccatcctgaa gaatggtgca gtgtctcggc accccatagg gaacctttgc
      301 catcaactcg gcaagatgca gcgtcgtgtt ggcatcgtgt cccacgccga ggagaagtac
      361 ctgcccatcg agttcatgga cacgggcgac cgggcttgca ggcgagtgag gtggcagggg
      421 caatggatca gagatgatct gctctgcctg tggccccgct gccgcaaagg caaatggatg
      481 ggcgctgcgc tttacatttg gcaggcgcca gaatgtgtca gagacaactc caaggtccgg
      541 tgtaacgggc gacgtggcag gatcgaacgg ctcgtcgtcc agacctgacc acgagggcat
      601 gacgagcgtc cctcccggac ccagcgcagc acgcagggcc tcgatcagtc caagtggccc
      661 atcttcgagg ggccggacgc tacggaagga gctgtggacc agcagcacac cgccgggggt
      721 aaccccaagg ttgagaagct gaccgatgag ctcggctttt cgccattcgt attgcacgac
      781 attgcactcc accgctgatg acatcagtcg atcatagcac gatcaacggc actgttgcaa
      841 atagtcggtg gtgataaact tatcatcccc ttttgctgat ggagctgcac atgaacccat
      901 tcaaaggccg gcattttcag cgtgacatca ttctgtgggc cgtacgctgg tactgcaaat
      961 accggcatca gttaccgtga gctgcaggtc gtcggatcct ttagtgaggg ttaattgcgg
     1021 ccgcgaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     1081 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     1141 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     1201 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     1261 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     1321 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     1381 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     1441 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     1501 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     1561 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     1621 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     1681 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     1741 aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     1801 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     1861 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     1921 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     1981 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     2041 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     2101 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     2161 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     2221 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2281 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2341 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     2401 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     2461 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     2521 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     2581 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     2641 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     2701 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     2761 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     2821 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2881 ggttcctggc cttttgctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2941 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3001 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gctgacttcc
     3061 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     3121 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     3181 aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc
     3241 gcacccgtca gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     3301 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     3361 cattataagc tgcaataaac aagttcccgc acgatcagcg tcccgccgac caggagcagt
     3421 gcggccagca ctgccgctaa cgcctggtcc tggtcccggt cctggtggtg catcagtcct
     3481 ccccgtgatc acttcggcac ccaccgtagt gatcaccccc gacagcggat caaggggttt
     3541 gcgggtcccg gtcggcgccg ggcgggggag gcaggagccg ccgacgctgc ctctgggacg
     3601 ggccggacgg cagggggacc ggcggccggg cgagctgcag ccgggggtcc ggcagggccg
     3661 gagcgggcgg aaccgtgctc tgacctgcgg cccgagtttc gtcacgtgac ggaatggaag
     3721 gctgctgcat ttcgtcacgt gacgtatctc ggcgagcgac tgccgacgcc acggcggaca
     3781 cgatcgcctc gcgctggcgc cgggcctcgt acgcccgctg gcggcaggag cggcggcagt
     3841 agtcccggct ccggccgacg ccggattgct tgatctccga gccgcaccag gcgcagagct
     3901 tcgcgccgtc ggcgtccctg ggggtggtgg tgctcatggc cgacgaccgt acgcggcacg
     3961 tctcgtagcg aggcgagtcg ggcgcgaggt accgcctgca cgaagtgccg gcggggccga
     4021 ccccgggcga gtaatcccag gattactccc gcggcttcga ccccggccgc cgtcgccgcg
     4081 tacgtcaccg acccccgccg tacgtcaccg ggatgacgta cggcgggggg gagcgagtta
     4141 gtgcgaagtg ggcccacttg cgagccgggc gatgtgccgg gcggcccgct cctggcggtc
     4201 gtcggcgtcg tcgtcctggt cgtcgtcctg ctctcgccgt cggcgtgcag ttgcttcctc
     4261 gcggcgctgg gcgagggcgg cgagcatgtc ggcgtacgcc tcggccacct cccccgccgt
     4321 gagcaccacc actgtgtcgg ccgcgtcggc cagcgccagg acctcccgca cccgttcgcc
     4381 cacggccgcc gaatcctcgt tgccgtcctt gccttcggcg gcccgggtcg cctcgaggtc
     4441 gagggcgcgg cgggtgaccg cgtgccatcc gtcctcggtc acggcgaccc cggcccgcag
     4501 ctccccgccg tcggcgtcgg ccgccaggag cagatcgagg tcgtcggcct cggtgtcgcc
     4561 gccgtcgagc ccgagcatct gccgcaggta gcgggtccat tcgatggccc ggcgtccccg
     4621 ggttgcccgc tcgtactcgt gccagcgcga gaggttccac tccagcgagc cgaccccggc
     4681 ggcgtcgtcc tcggtcatgc cgccggtcag gtccccgatc cgtccgagga gttcgaacgg
     4741 ggcgacgttc ccgccggtcg ccgtcttgag gtcggcgcgg gcgagttcga gggcgggcgc
     4801 cttcccgtcc tgggtcttgg cgatgtactc ggcgaggtcg ttggcgtcgc gctcggtctc
     4861 cagccgcttg aagtcgacgc cgtgccggtc gtcgggcgtg aaggcggggt tgaccttgcg
     4921 cagggcggcg gtccacacgg accgccagtg cccctgccac tcgtcgagcg cggcgccggt
     4981 cggctcgaag gtggcgacga tctgcttcgc ggaccgctcc ccctcggtcc ggccgccgac
     5041 caggacgatc gcgtggatgt gcgggtgcca gccgttgatc tgccccacgg tgacttcggt
     5101 cgcgcggatc atgccgacgt acccgatccg gtctcggatg ccctcgcggt cggcggcccg
     5161 gtgcccgtcc ttggcccggc gtccggccca cgtgccgccc gtgatcagtc gctggtaggc
     5221 gcccggccgc cgggggctgt ccggcgtctt ccgggtgccc tggagggcgt ccatgaggtc
     5281 cgcgagccgg tccgtgtgcc catggcgggc cgtgaaggtg accaggtagg cggtcccccc
     5341 gcgcttgatc cactcgacca cggcggcggt gatctcctcg gcccgcttgt gccggatcgt
     5401 ggcggcgcag accgggcaga gccagatccg cccgcaccgc atcaggccca ggaccacgga
     5461 cgttccggcc gccgtctggg cgacgatcac gccggaggca gggtccatca gggcgcggcc
     5521 gcagcccttg cacgcggcgt ccccgctgat ccgccacagc gtccggcggc ggctgtaccg
     5581 ggcggctttc cgcagtcggg cagcgtcgct ccgcgacgtg cttcctactt ccgagaggct
     5641 gtcgcctctc gggctctccc catccacccc gtccggagaa accgcaggtc ggaggggtgc
     5701 gggaaactct gttgtttctt tcccaaggtg ttcgcttttg cctcgggcgg catctcgcgt
     5761 cacacgcgcg atcgcccgct tcgctgccat ccggcagcgg tctgagcagt agatacgcgg
     5821 ccgtttgccc ggtgtgtggg caattgcggt cccgcagtgg cagcggggcc cggcgggccg
     5881 atctggcaat gcctcggcat cgctccgtac tctgggcacg agcaacgttc ctgtctcgcc
     5941 cggctaaggg gcgcgagtct gggagcggac gggtcggagg tgcgaagtcc ggcccgttgc
     6001 tctttggtct ggtgggaatc ctggcaccaa tcgggccaga ggttccctcc gccactcccg
     6061 acgccccttg gggctggtgt gacttggagg gccgaagaga gccccgccgg tgatccggcg
     6121 gggctttgac gtgcggtcag tgcgtgtgtc ggcgagcgat ggccacgagg ccctggaagc
     6181 cgagcggtcc ggcgaagtcg gcccagtcgc aaccgggctc agcgcagtgg gcggaccagc
     6241 caccgccgtt gtgggtcctg gaccaggttc acggtcccct cggtcaggcg tccgtcgaag
     6301 tcggtcatgg tcggtctcct ggtgggtggg ggcggggcgc cagcacgaag tgccggcgcc
     6361 ccgcgggggt tggtcgggtc aggcgccgaa ccggcgggcg gcggcggcga ccaggccgtc
     6421 ggcggcggcc atggcgcggt cgcggtcggt ggtgagggcg gtgcggtcgg cggcggccca
     6481 gtcctgctct cacgcaacgt ctacgtggac gctcagggcg acacgatcga ggtcggggag
     6541 tccgtcgctc cggcgggccg ctggcgcgtc caccgggact gatcaaggcg aatacttcat
     6601 atggccctgc aggcggaagt caggtagaca cgacttccgc tagtccttgc aaggtctgct
     6661 gacgtgaggc ggggcggtcg tttttgaccg ccctgccttc gtcatgtagg ctcgctcgct
     6721 gtgcctggcg tgtcatcaga cgcccaggtc ccggtgccgt gaggcccggg ccatcgagcc
     6781 ggtggtacgt ggctgcggtc cccttgtgag ggctgcgcgc cgtgtgctgt ccggcgcgca
     6841 cagccttgaa tccacccgcg ggggccggcc ggtctccgtg agctcgagta gacgacggag
     6901 acgtaatgga ggcctccccc gcctccggcc cccgccacct gatggacccg cacatcttca
     6961 cctccaactt caacaacggc atcggccgcc acaagaccta cctgtgctac gaggtcgagc
     7021 ggctggacaa cggcacctcc gtcaagatgg accagcaccg gggcttcctg cacaaccagg
     7081 ccaagaacct gctgtgcggc ttctacggcc gccacgccga gctgcggttc ctggacctgg
     7141 tcccctccct gcagctggac cccgcccaga tctaccgcgt cacctggttc atctcctggt
     7201 ccccgtgctt ctcctggggc tgcgccggcg aggtccgggc cttcctgcag gagaacaccc
     7261 acgtccgcct gcggatcttc gccgcccgca tcttcgacta cgaccccctg tacaaggaag
     7321 ccctgcagat gctgcgggac gccggcgccc aggtctccat catgacctac gacgagttca
     7381 agcactgctg ggacaccttc gtggaccacc agggctgccc cttccagccc tgggacggcc
     7441 tggacgagca ctcccaggcc ctgtccggcc gcctgcgggc catcctgcag aaccagggca
     7501 actccggctc ggagaccccg ggcacctcgg agtccgccac ccccgagtcc tccggctcgg
     7561 agaccccggg cacctcggag tccgccaccc cggagtccga caagaagtac agcatcggcc
     7621 tggccatcgg caccaacagc gtgggctggg cggtcatcac cgacgagtac aaggtcccct
     7681 ccaagaagtt caaggtcctg ggcaacaccg accggcactc gatcaagaag aacctgatcg
     7741 gcgccctgct cttcgacagc ggcgagaccg ccgaggcgac ccgcctgaag cggaccgcgc
     7801 gccggcgcta cacccggcgc aagaaccgca tctgctacct ccaggaaatc ttctccaacg
     7861 agatggccaa ggtcgacgac tcgttcttcc accggctcga ggagagcttc ctggtggagg
     7921 aggacaagaa gcacgagcgc cacccgatct tcggcaacat cgtcgacgag gtggcctacc
     7981 acgagaagta ccccaccatc taccacctcc gcaagaagct ggtcgactcg accgacaagg
     8041 cggacctgcg gctcatctac ctggccctcg cgcacatgat caagttccgc ggccacttcc
     8101 tcatcgaggg cgacctgaac ccggacaact ccgacgtcga caagctcttc atccagctgg
     8161 tgcagaccta caaccagctg ttcgaggaga accccatcaa cgccagcggc gtcgacgcca
     8221 aggcgatcct ctccgcgcgc ctgagcaagt cccggcgcct ggagaacctc atcgcccagc
     8281 tgccgggcga gaagaagaac ggcctcttcg gcaacctgat cgcgctgtcg ctcggcctga
     8341 cccccaactt caagagcaac ttcgacctgg ccgaggacgc gaagctccag ctgtccaagg
     8401 acacctacga cgacgacctg gacaacctgc tcgcccagat cggcgaccag tacgcggacc
     8461 tcttcctggc cgcgaagaac ctctcggacg ccatcctgct cagcgacatc ctgcgggtca
     8521 acaccgagat caccaaggcc ccgctgtcgg cgagcatgat caagcggtac gacgagcacc
     8581 accaggacct gaccctgctc aaggccctcg tgcgccagca gctgcccgag aagtacaagg
     8641 aaatcttctt cgaccagtcc aagaacggct acgccggcta catcgacggc ggcgcgtcgc
     8701 aggaggagtt ctacaagttc atcaagccga tcctggagaa gatggacggc accgaggagc
     8761 tgctcgtcaa gctgaaccgc gaggacctgc tccgcaagca gcggaccttc gacaacggct
     8821 ccatcccgca ccagatccac ctgggcgagc tgcacgccat cctccggcgc caggaggact
     8881 tctacccctt cctgaaggac aaccgcgaga agatcgagaa gatcctgacc ttccgcatcc
     8941 cgtactacgt cggccccctg gcccgcggca actcccggtt cgcgtggatg acccggaagt
     9001 cggaggagac catcaccccg tggaacttcg aggaggtcgt ggacaagggc gcctccgccc
     9061 agtcgttcat cgagcgcatg accaacttcg acaagaacct cccgaacgag aaggtcctgc
     9121 ccaagcactc cctgctctac gagtacttca ccgtgtacaa cgagctgacc aaggtcaagt
     9181 acgtgaccga gggcatgcgg aagccggcct tcctgtcggg cgagcagaag aaggcgatcg
     9241 tcgacctgct cttcaagacc aaccgcaagg tcaccgtgaa gcagctgaag gaggactact
     9301 tcaagaagat cgagtgcttc gactccgtcg agatcagcgg cgtggaggac cgcttcaacg
     9361 cctccctggg cacctaccac gacctgctca agatcatcaa ggacaaggac ttcctcgaca
     9421 acgaggagaa cgaggacatc ctggaggaca tcgtcctcac cctgaccctc ttcgaggacc
     9481 gcgagatgat cgaggagcgg ctcaagacct acgcccacct gttcgacgac aaggtgatga
     9541 agcagctgaa gcggcgccgg tacaccggct ggggccgcct ctcccggaag ctgatcaacg
     9601 gcatccggga caagcagagc ggcaagacca tcctggactt cctcaagtcc gacggcttcg
     9661 ccaaccgcgc gttcgccgcg ctcatcgccg acgacagcct gaccttcaag gaggacatcc
     9721 agaaggccca ggtctcgggc cagggcgaca gcctccacga gcacatcgcc aacctggcgg
     9781 gctccccggc gatcaagaag ggcatcctcc agaccgtcaa ggtcgtggac gagctggtca
     9841 aggtgatggg ccgccacaag cccgagaaca tcgtgatcga gatggcccgg gagaaccaga
     9901 ccacccagaa gggccagaag aactcgcgcg agcggatgaa gcggatcgag gagggcatca
     9961 aggagctggg cagccagatc ctgaaggagc acccggtcga gaacacccag ctccagaacg
    10021 agaagctgta cctctactac ctccagaacg gccgcgacat gtacgtggac caggagctgg
    10081 acatcaaccg gctgtccgac tacgacgtcg acgccatcgt gccgcagtcc ttcctgaagg
    10141 acgactcgat cgacaacaag gtcctgaccc gctcggacaa gaaccggggc aagtccgaca
    10201 acgtgccctc ggaggaggtc gtgaagaaga tgaagaacta ctggcgccag ctgctcaacg
    10261 ccaagctcat cacccagcgc aagttcgaca acctgaccaa ggccgagcgg ggcggcctga
    10321 gcgagctgga caaggcgggc ttcatcaagc gccagctggt cgagacccgg cagatcacca
    10381 agcacgtggc ccagatcctg gactcccgga tgaacaccaa gtacgacgag aacgacaagc
    10441 tgatccgcga ggtcaaggtg atcaccctca agagcaagct ggtctccgac ttccgcaagg
    10501 acttccagtt ctacaaggtc cgggagatca acaactacca ccacgcccac gacgcgtacc
    10561 tgaacgccgt cgtgggcacc gcgctgatca agaagtaccc gaagctggag tccgagttcg
    10621 tctacggcga ctacaaggtc tacgacgtgc gcaagatgat cgccaagagc gagcaggaga
    10681 tcggcaaggc caccgcgaag tacttcttct actccaacat catgaacttc ttcaagaccg
    10741 agatcaccct ggccaacggc gagatccgca agcggcccct gatcgagacc aacggcgaga
    10801 ccggcgagat cgtctgggac aagggccgcg acttcgccac cgtccggaag gtgctgtcga
    10861 tgccgcaggt caacatcgtg aagaagaccg aggtgcagac cggcggcttc agcaaggagt
    10921 ccatccggcc caagcgcaac agcgacaagc tgatcgcccg gaagaaggac tgggacccga
    10981 agaagtacgg cggcttcgtc agccccaccg tcgcctactc cgtgctggtc gtggcgaagg
    11041 tcgagaaggg caagagcaag aagctgaagt ccgtgaagga gctgctcggc atcaccatca
    11101 tggagcgctc ctcgttcgag aagaacccga tcgacttcct ggaggccaag ggctacaagg
    11161 aggtcaagaa ggacctcatc atcaagctgc ccaagtacag cctgttcgag ctggagaacg
    11221 gccgcaagcg gatgctcgcc tccgcgcggt tcctgcaaaa gggcaacgag ctggccctcc
    11281 cgtcgaagta cgtcaacttc ctgtacctcg cgtcccacta cgagaagctg aagggctcgc
    11341 ccgaggacaa cgagcagaag cagctcttcg tggagcagca caagcactac ctggacgaga
    11401 tcatcgagca gatcagcgag ttcagcaagc gcgtcatcct ggccgacgcg aacctcgaca
    11461 aggtgctgtc cgcctacaac aagcaccgcg acaagccgat ccgggagcag gcggagaaca
    11521 tcatccacct gttcaccctc accaacctgg gtgccccgcg ggccttcaag tacttcgaca
    11581 ccaccatcga ccgcaaggtc taccgctcca ccaaggaggt cctcgacgcg accctgatcc
    11641 accagagcat caccggcctg tacgagaccc gcatcgacct gagccagctg ggcggcgact
    11701 cgggcggctc caccaacctg tcggacatca tcgagaagga gaccggcaag cagctggtga
    11761 tccaggagtc catcctgatg ctgccggagg aagtggagga agtgatcggc aacaagccgg
    11821 agtcggacat cctggtgcac accgcctacg acgagtcgac cgacgagaac gtcatgctgc
    11881 tgacctcgga cgccccggag tacaagccgt gggccctggt catccaggac tccaacggcg
    11941 agaacaagat caagatgctg tgagaattca gatctacgcg ttccccgcaa aagcggcctt
    12001 tgactccctg caagcctcag cgaccgaata tatcggttat gcgtgggcga tggttgttgt
    12061 cattgtcggc gcaactatcg gtatcaagct gtttaagaaa ttcacctcga aagcaagctg
    12121 ataaaccgat acaattaaag gctccttttg gagccttttt ttaagcttgc atgcctgcag
    12181 gtcgacggat cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg
    12241 tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc
    12301 cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg
    12361 ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc
    12421 tgcgaggctg gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa
    12481 ccaagccaac caggaagggc agcccaccta tcaaggtgta ctgccttcca gacgaacgaa
    12541 gagcgattga ggaaaaggcg gcggcggccg gcatgagcct gtcggcctac ctgctggccg
    12601 tcggccaggg ctacaaaatc acgggcgtcg tggactatga gcacgtccgc gagctggccc
    12661 gcatcaatgg cgacctgggc cgcctgggcg gcctgctgaa actctggctc accgacgacc
    12721 cgcgcacggc gcggttcggt gatgccacga tcctcgccct gctggcgaag atcgaagaga
    12781 agcaggacga gcttggcaag gtcatgatgg gcgtggtccg cccgagggca gagccatgac
    12841 ttttttagcc gctaaaacgg ccggggggtg cgcgtgattg ccaagcacgt ccccatgcgc
    12901 tccatcaaga agagcgactt cgcggagctg gtgaagtaca tcaccgacga gcaaggcaag
    12961 accgatcccc ggggacctgc aggtcgactc tagagtttta gagctagaaa tagcaagtta
    13021 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc taaaaaaaaa
    13081 ccccgcccct gacagggcgg ggtttttttt gtagcctcag gagttgtagc tgttcacatt
    13141 cgaacggtct ctgctttgac aacatgctgt gcggtgttgt aaagtcgtgg ccgctagcta
    13201 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
    13261 ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg
    13321 acagacgtcg cggtgagttc aggctttttc atatctcatt gcccccggac gagcgtctgc
    13381 tccgccattc gccgtccgcc gtgccaatcg gatcagccgt ccaaatgcgg gattttcgtt
    13441 agtcggaggc caaacggcat tgagcgtcag catatcatca gcgagctgaa gaaagacaat
    13501 ccccgatccg ctccacgtgt tgccccagca atcagcgcga ccttgcccct ccaacgtcat
    13561 ctcgttctcc gctcatgagc
//
