LOCUS       1804_GB5_pLenti-        8305 bp DNA     circular SYN 20-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8305)
  AUTHORS   Nicole
  TITLE     Direct Submission
  JOURNAL   Exported Mar 12, 2024 from SnapGene Viewer 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     enhancer        1816..2119
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2274..2294
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(2302..2324)
                     /label=Tet removal reverse
     protein_bind    2325..2343
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2346..2364
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2365..2384
                     /label=Tet removal foward*
     primer_bind     complement(2397..2418)
                     /label=Gibson Rev
     primer_bind     2399..2438
                     /label=pLp Cloning Fwd
     primer_bind     2399..2418
                     /label=Overlap
     CDS             2432..2455
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2495..2518
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2525..3709
                     /codon_start=1
                     /label=GNB5
                     /translation="MCDQTFLVNVFGSCDKCFKQRALRPVFKKSQQLSYCSTCAEIMAT
                     EGLHENETLASLKSEAESLKGKLEEERAKLHDVELHQVAERVEALGQFVMKTRRTLKGH
                     GNKVLCMDWCKDKRRIVSSSQDGKVIVWDSFTTNKEHAVTMPCTWVMACAYAPSGCAIA
                     CGGLDNKCSVYPLTFDKNENMAAKKKSVAMHTNYLSACSFTNSDMQILTASGDGTCALW
                     DVESGQLLQSFHGHGADVLCLDLAPSETGNTFVSGGCDKKAMVWDMRSGQCVQAFETHE
                     SDINSVRYYPSGDAFASGSDDATCRLYDLRADREVAIYSKESIIFGASSVDFSLSGRLL
                     FAGYNDYTINVWDVLKGSRVSILFGHENRVSTLRVSPDGTAFCSGSWDHTLRVWA"
     primer_bind     complement(2863..2881)
                     /label=Gb5_S81L_R.3
                     /note="Mutagenesis Primer for S81L mutation - reverse"
     primer_bind     2882..2902
                     /label=Gb5_S81L_F.3
                     /note="Mutagenesis Primer for S81L mutation"
     CDS             3716..3751
                     /codon_start=1
                     /label=HPC4
                     /translation="EDQVDPRLIDGK"
     primer_bind     complement(3742..3784)
                     /label=pLp Cloning Rev
     primer_bind     3765..3788
                     /label=Gibson Fwd
     primer_bind     complement(3765..3784)
                     /label=Overlap 2
     primer_bind     complement(3782..3799)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     3803..3824
                     /label=BCL Hifi Fwd
     primer_bind     complement(3811..3831)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3816..4145
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3996..4131
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4058..4077
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4081..4092)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4131..4154)
                     /label=Puro Swap Rev
     CDS             4155..4754
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     4155..4174
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4651..4671
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(4744..4763)
                     /label=puro Hifi Rev
     primer_bind     4744..4762
                     /label=Primer 1
     LTR             4826..5059
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5131..5252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5168..5187)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5222..5241
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5292..5427
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5354..5373
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(5377..5388)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(5447..5466)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5448..5466)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5476..5493)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5476..5492)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5485..5507)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5634..6089
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5721..5740)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5931..5952
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6115..6219
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6220..6288,6289..7080)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6438..6457)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7251..7839
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7740..7759
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7993..8010
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8127..8148
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8163..8168,8169..8186,8187..8193)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8201..8217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8206..8228
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8225..8241
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8225..8241
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8260..8280
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8262..8280
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag
     2341 agatctccct atcagtgata gagatcgtcg actagtggat ccagtgtggt ggaattctgc
     2401 agatatccag cacagtggtg caggatccat gtggagccac ccgcagttcg aaaaaggagg
     2461 tggttctggt ggaggttctg gtggttcatc agcttggagc cacccgcagt tcgaaaaagc
     2521 tgctatgtgt gatcagacct ttctcgttaa tgtatttggc tcatgtgaca aatgtttcaa
     2581 acaacgagct ctgagaccag ttttcaagaa gtctcaacaa ctcagctact gttcaacatg
     2641 tgcagaaatt atggcaaccg aggggctgca cgagaacgag acgctggcgt cgctgaagag
     2701 cgaggccgag agcctcaagg gcaagctgga ggaggagcga gccaagctgc acgatgtgga
     2761 gctgcaccag gtggcggagc gggtggaggc cctggggcag tttgtcatga agaccagaag
     2821 gaccctcaaa ggccacggga acaaagtcct gtgcatggac tggtgcaaag ataagaggag
     2881 gatcgtgagc tcgtcacagg atgggaaggt gatcgtgtgg gattccttca ccacaaacaa
     2941 ggagcacgcg gtcaccatgc cctgcacgtg ggtgatggca tgtgcttatg ccccatcggg
     3001 atgtgccatt gcttgtggtg gtttggataa taagtgttct gtgtacccct tgacgtttga
     3061 caaaaatgaa aacatggctg ccaaaaagaa gtctgttgct atgcacacca actacctgtc
     3121 ggcctgcagc ttcaccaact ctgacatgca gatcctgaca gcgagcggcg atggcacatg
     3181 tgccctgtgg gacgtggaga gcgggcagct gctgcagagc ttccacggac atggggctga
     3241 cgtcctctgc ttggacctgg ccccctcaga aactggaaac accttcgtgt ctgggggatg
     3301 tgacaagaaa gccatggtgt gggacatgcg ctccggccag tgcgtgcagg cctttgaaac
     3361 acatgaatct gacatcaaca gtgtccggta ctaccccagt ggagatgcct ttgcttcagg
     3421 gtcagatgac gctacgtgtc gcctctatga cctgcgggca gatagggagg ttgccatcta
     3481 ttccaaagaa agcatcatat ttggagcatc cagcgtggac ttctccctca gtggtcgcct
     3541 gctgtttgct ggatacaatg attacactat caacgtctgg gatgttctca aagggtcccg
     3601 ggtctccatc ctgtttggac atgaaaaccg cgttagcact ctacgagttt cccccgatgg
     3661 gactgctttc tgctctggat catgggatca taccctcaga gtctgggccg gtggagagga
     3721 ccaggtggac cccaggctga tcgacggcaa gtaagaattc aaggttaaac ctgctgatca
     3781 gcctcgactg tgccttctag tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3841 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3901 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3961 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4021 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4081 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     4141 gcaaaaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     4201 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     4261 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     4321 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     4381 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     4441 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     4501 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     4561 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     4621 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     4681 tcaccgtcac cgccgacgtc gagtgcccga aggaccgcgc gacctggtgc atgacccgca
     4741 agcccggtgc ctgaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     4801 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     4861 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     4921 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     4981 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     5041 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     5101 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     5161 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5221 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     5281 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     5341 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     5401 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     5461 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     5521 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     5581 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     5641 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5701 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5761 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5821 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5881 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5941 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6001 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6061 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     6121 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6181 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6241 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6301 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6361 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6421 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6481 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     6541 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6601 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6661 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6721 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6781 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6841 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6901 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6961 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7021 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7081 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7141 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7201 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7261 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7321 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7381 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7441 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7501 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7561 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7621 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7681 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7741 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7801 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7861 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7921 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     7981 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     8041 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     8101 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     8161 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     8221 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     8281 gaacaaaagc tggagctgca agctt
//
