LOCUS       1807_Phlp1_pLent        7795 bp DNA     circular SYN 11-APR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Nicole
  TITLE     Direct Submission
  JOURNAL   Exported Mar 12, 2024 from SnapGene Viewer 7.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7795
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     enhancer        1816..2119
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2274..2294
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(2302..2324)
                     /label=Tet removal reverse
     protein_bind    2325..2343
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2346..2364
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2365..2384
                     /label=Tet removal foward*
     primer_bind     complement(2397..2418)
                     /label=pLenti-puro Gibson Rev
     primer_bind     2399..2438
                     /label=pLp cloning Fwd
     primer_bind     2399..2418
                     /label=Overlap
     regulatory      2422..2431
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2428..3335
                     /codon_start=1
                     /label=hPhlp1
                     /translation="MGTTLDDKLLGEKLQYYYSSSEDEDSDHEDKDRGRCAPASSSVPA
                     EAELAGEGISVNTGPKGVINDWRRFKQLETEQREEQCREMERLIKKLSMTCRSHLDEEE
                     EQQKQKDLQEKISGKMTLKEFAIMNEDQDDEEFLQQYRKQRMEEMRQQLHKGPQFKQVF
                     EISSGEGFLDMIDKEQKSIVIMVHIYEDGIPGTEAMNGCMICLAAEYPAVKFCKVKSSV
                     IGASSQFTRNALPALLIYKGGELIGNFVRVTDQLGDDFFAVDLEAFLQEFGLLPEKEVL
                     VLTSVRNSATCHSEDSDLEID"
     primer_bind     3046..3062
                     /label=Phlp1 Int Fwd
     CDS             3355..3384
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3400..3417
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3412..3455)
                     /label=pLp cloning rev
     primer_bind     3436..3459
                     /label=pLenti-puro Gibson Fwd
     primer_bind     complement(3436..3455)
                     /label=Overlap 2
     primer_bind     complement(3453..3470)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     3474..3495
                     /label=BCL Hifi Fwd
     primer_bind     complement(3482..3502)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3487..3816
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3667..3802
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3729..3748
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(3752..3763)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(3802..3825)
                     /label=Puro Swap Rev
     primer_bind     3806..3844
                     /label=Blast swap Fwd
     CDS             3826..3838
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="NPGP"
     CDS             3839..4237
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(4223..4264)
                     /label=Blast swap rev
     primer_bind     4245..4269
                     /label=Puro Swap Fwd
     primer_bind     complement(4272..4291)
                     /label=pLp Resistance Rev
     LTR             4316..4549
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4621..4742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4658..4677)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4712..4731
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4782..4917
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4844..4863
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4867..4878)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4937..4956)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4938..4956)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4966..4983)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4966..4982)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4975..4997)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5124..5579
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5211..5230)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5421..5442
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5605..5709
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5710..5778,5779..6570)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5928..5947)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6741..7329
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7230..7249
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7483..7500
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7617..7638
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7653..7658,7659..7676,7677..7683)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7691..7707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7696..7718
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7715..7731
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7715..7731
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7750..7770
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7752..7770
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     1801 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1861 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     1921 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     1981 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2041 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2101 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2161 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2221 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2281 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag
     2341 agatctccct atcagtgata gagatcgtcg actagtggat ccagtgtggt ggaattctgc
     2401 agatatccag cacagtggtt cgccaccatg ggaaccaccc ttgatgataa gttgctgggg
     2461 gagaaactgc agtactacta tagcagcagt gaggatgagg acagtgacca cgaggacaag
     2521 gaccgaggca gatgtgcccc agccagcagt tctgtgcctg cagaggctga gctggcaggc
     2581 gaaggcatct cagttaacac aggcccaaaa ggtgtgatca atgactggcg ccgcttcaag
     2641 cagttggaga cagagcagag ggaggagcag tgccgggaga tggaaaggct gatcaagaag
     2701 ctgtcaatga cttgcaggtc ccatctggat gaagaggagg agcaacagaa acagaaagac
     2761 ctccaggaga agatcagtgg gaagatgact ctgaaggagt ttgccataat gaatgaggac
     2821 caagatgatg aagagtttct gcagcagtac cggaagcagc gaatggaaga gatgcggcag
     2881 cagcttcaca aggggcccca attcaagcag gtttttgaga tctccagtgg agaagggttt
     2941 ttagacatga ttgataaaga acagaaaagc attgtcatca tggttcatat ttatgaggat
     3001 ggcattccag ggaccgaagc catgaatggt tgcatgatct gccttgccgc agagtaccca
     3061 gctgtcaagt tctgcaaggt gaagagctca gttattggcg ccagcagtca gttcaccagg
     3121 aatgcccttc ctgccctgct gatctataag gggggtgaat tgatcggcaa ttttgttcgt
     3181 gttactgacc agctggggga tgatttcttt gctgtggacc ttgaagcttt tctccaggaa
     3241 tttggattac tcccagaaaa ggaagtcttg gtgctgacat ctgtgcgtaa ctctgccacg
     3301 tgtcacagtg aggatagcga cctggaaata gatgctctag agggcccgcg gttcgaacaa
     3361 aaactcatct cagaagagga tctgaatatg cataccggtc atcatcacca tcaccattga
     3421 gtttaaaccc gctgattaaa cctgctgatc agcctcgact gtgccttcta gtcgaccctg
     3481 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3541 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     3601 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     3661 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     3721 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     3781 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttaccgaatc ctggaccgat
     3841 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
     3901 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg
     3961 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt
     4021 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
     4081 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca
     4141 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg
     4201 tgaattgctg ccctctggtt atgtgtggga gggctaagaa ttcgggtacc tttaagacca
     4261 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     4321 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     4381 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     4441 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     4501 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     4561 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     4621 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4681 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4741 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     4801 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4861 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4921 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     4981 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     5041 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     5101 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5161 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5221 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5281 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5341 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5401 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5461 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5521 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     5581 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5641 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5701 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5761 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5821 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5881 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5941 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6001 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6061 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6121 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6181 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6241 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6301 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6361 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6421 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6481 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6541 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6601 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6661 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6721 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6781 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6841 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6901 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6961 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7021 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7081 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7141 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7201 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7261 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7321 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7381 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7441 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7501 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7561 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7621 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7681 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7741 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agctt
//
