LOCUS       pK2_K3                  2188 bp    DNA        circular     12-MAR-2024
COMMENT     pJC221_J6-CcdB-J9 from 1 to 2892
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             246..1256
                     /locus_tag="Sm/Sp\no\DraIII"
                     /label="Sm/Sp\no\DraIII"
                     /ApEinfo_label="Sm/Sp\no\DraIII"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1416..2030
                     /locus_tag="rep\(pMB1)"
                     /label="rep\(pMB1)"
                     /ApEinfo_label="rep\(pMB1)"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1351..2033
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4..9
                     /locus_tag="BsaI Binding site"
                     /label="BsaI Binding site"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(37..42)
                     /locus_tag="BsaI Binding site(1)"
                     /label="BsaI Binding site(1)"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    11..14
                     /locus_tag="K2"
                     /label="K2"
                     /ApEinfo_label="K2"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    32..35
                     /locus_tag="K3"
                     /label="K3"
                     /ApEinfo_label="K3"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 agcggtctcg AAGCCTAGGC TGCACGGGCA TGCACagaga ccgtggacga aagggcctcg
       61 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
      121 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
      181 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      241 agagtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
      301 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
      361 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
      421 atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga
      481 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
      541 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
      601 gcgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
      661 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
      721 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
      781 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
      841 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
      901 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
      961 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     1021 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     1081 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     1141 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     1201 tggaagaatt tgtccattac gtaaaaggcg agatcaccaa ggtagtcggc aaataactgt
     1261 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     1321 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     1381 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     1441 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     1501 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     1561 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     1621 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     1681 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     1741 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1801 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     1861 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     1921 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     1981 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     2041 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     2101 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     2161 ccgagcgcag cgagtcagtg agcgagga   
//
