LOCUS       pK6_K7                  2193 bp    DNA        circular     12-MAR-2024
COMMENT     pJC221_J6-CcdB-J9 from 1 to 2892
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             248..1258
                     /locus_tag="Sm/Sp\no\DraIII"
                     /label="Sm/Sp\no\DraIII"
                     /ApEinfo_label="Sm/Sp\no\DraIII"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1418..2032
                     /locus_tag="rep\(pMB1)"
                     /label="rep\(pMB1)"
                     /ApEinfo_label="rep\(pMB1)"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1353..2035
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1..6
                     /locus_tag="BsaI Binding site"
                     /label="BsaI Binding site"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(39..44)
                     /locus_tag="BsaI Binding site(1)"
                     /label="BsaI Binding site(1)"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8..11
                     /locus_tag="K6"
                     /label="K6"
                     /ApEinfo_label="K6"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    34..37
                     /locus_tag="K7"
                     /label="K7"
                     /ApEinfo_label="K7"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ggtctcgTGG CGGTGGTAAT GCCATGTAAT ATGACTCaga gaccgtggac gaaagggcct
       61 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
      121 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      181 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      241 gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
      301 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg
      361 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa
      421 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga gggaagcggt
      481 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga
      541 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca
      601 cagcgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc
      661 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc
      721 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg
      781 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc
      841 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt
      901 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc
      961 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa
     1021 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa
     1081 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact
     1141 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca
     1201 gttggaagaa tttgtccatt acgtaaaagg cgagatcacc aaggtagtcg gcaaataact
     1261 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1321 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1381 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1441 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1501 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1561 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1621 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1681 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1741 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1801 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1861 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1921 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1981 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     2041 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     2101 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     2161 gaccgagcgc agcgagtcag tgagcgagga agc  
//
