LOCUS       pK2_J11                 2189 bp    DNA        circular     12-MAR-2024
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2156..2161
                     /locus_tag="BsaI Binding site"
                     /label="BsaI Binding site"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2163..2166
                     /locus_tag="K2"
                     /label="K2"
                     /ApEinfo_label="K2"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2185..2188
                     /locus_tag="J11"
                     /label="J11"
                     /ApEinfo_label="J11"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1..6)
                     /locus_tag="BsaI Binding site(1)"
                     /label="BsaI Binding site(1)"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             210..1220
                     /locus_tag="Sm/Sp\no\DraIII"
                     /label="Sm/Sp\no\DraIII"
                     /ApEinfo_label="Sm/Sp\no\DraIII"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      1315..1997
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1380..1994
                     /locus_tag="rep\(pMB1)"
                     /label="rep\(pMB1)"
                     /ApEinfo_label="rep\(pMB1)"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 gagaccgtgg acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
       61 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      121 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      181 tgcttcaata atattgaaaa aggaagagta tgcgctcacg caactggtcc agaaccttga
      241 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
      301 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
      361 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
      421 taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
      481 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
      541 tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg accgtaaggc
      601 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
      661 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
      721 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
      781 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
      841 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
      901 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
      961 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     1021 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     1081 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     1141 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa ggcgagatca
     1201 ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt tagattgatt
     1261 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     1321 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1381 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1441 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1501 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     1561 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1621 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1681 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1741 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1801 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1861 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1921 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1981 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     2041 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2101 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggtct
     2161 cAAAGCCTAG GCTGCACGGG CATCCGCTa   
//
