LOCUS       pK4_J11                 2196 bp    DNA        circular     12-MAR-2024
COMMENT     New DNA(2) from 1 to 2892
COMMENT     New DNA(1) from 1 to 2892
COMMENT     New DNA from 1 to 2892
COMMENT     pJC221_J6-CcdB-J9 from 1 to 2892
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             251..1261
                     /locus_tag="Sm/Sp\no\DraIII"
                     /label="Sm/Sp\no\DraIII"
                     /ApEinfo_label="Sm/Sp\no\DraIII"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1421..2035
                     /locus_tag="rep\(pMB1)"
                     /label="rep\(pMB1)"
                     /ApEinfo_label="rep\(pMB1)"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      1356..2038
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1..6
                     /locus_tag="BsaI Binding site"
                     /label="BsaI Binding site"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(42..47)
                     /locus_tag="BsaI Binding site(1)"
                     /label="BsaI Binding site(1)"
                     /ApEinfo_label="BsaI Binding site"
                     /ApEinfo_fwdcolor="#cccccc"
                     /ApEinfo_revcolor="#333333"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    8..11
                     /locus_tag="K4"
                     /label="K4"
                     /ApEinfo_label="K4"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    37..40
                     /locus_tag="J11"
                     /label="J11"
                     /ApEinfo_label="J11"
                     /ApEinfo_fwdcolor="#cc66fe"
                     /ApEinfo_revcolor="#cc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ggtctcgCCA CCAATTTACA TCTTTATTTA TTAACGCGCT agagaccgtg gacgaaaggg
       61 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
      121 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      181 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      241 aaggaagagt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
      301 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
      361 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
      421 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc
      481 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
      541 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
      601 acacagcgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
      661 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
      721 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
      781 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
      841 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
      901 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
      961 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     1021 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     1081 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     1141 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     1201 tcagttggaa gaatttgtcc attacgtaaa aggcgagatc accaaggtag tcggcaaata
     1261 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     1321 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1381 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1441 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1501 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1561 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1621 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1681 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1741 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1801 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1861 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1921 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1981 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2041 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2101 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     2161 aacgaccgag cgcagcgagt cagtgagcga ggaagc  
//
