LOCUS       pCW57-CMV-MAAP-SL01     8273 bp ds-DNA     circular     09-MAR-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     source          1..8273
                     /label="source"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="organism: synthetic DNA construct mol_type: other DNA"
     misc_feature    41..158
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="note: central polypurine tract and central termination sequence of HIV-1"
     misc_feature    207..586
                     /label="CMV enhancer from pcDNA3"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        587..790
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     835..854
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    867..872
                     /label="KpnI"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    873..878
                     /label="AgeI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    879..884
                     /label="KOZAK"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    885..1184
                     /label="MAAP-SL01 ORF"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             885..1184
                     /label="Translation 885-1184"
                     /translation="MAHHHQGPQRGIRATAGVLCFLGTSTSDPSTDTSRESRSTRQTPRPLSMSKPTTGSPTADNTRTSSATSPTRSLGAPKGCYVFRGQTRTSSLPGEKEGS*"
     promoter        1215..1725
                     /label="hPGK promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: human phosphoglycerate kinase 1 promoter"
     primer_bind     1644..1662
                     /label="hPGK-F"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="note: Human PGK promoter, forward primer"
     primer_bind     complement(1735..1754)
                     /label="Puro-R"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: Puromycin resistance gene, reverse primer. Also called puro-variant-R"
     CDS             1735..2331
                     /label="PuroR"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: pac from Streptomyces alboniger product: puromycin N-acetyltransferase note: confers resistance to puromycin translation: MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             1735..2331
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     2231..2251
                     /label="Puro-F"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="note: Puromycin resistance gene, forward primer"
     CDS             2332..2385
                     /label="T2A"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="product: 2A peptide from Thosea asigna virus capsid protein note: Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. translation: EGRGSLLTCGDVEENPGP"
     CDS             2332..2385
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2386..3132
                     /label="rtTA-Advanced"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="product: improved tetracycline-controlled transactivator note: In the Tet-On(R) system, rtTA-Advanced binds to the Tet-responsive element and stimulates transcription only inthe presence of tetracycline or doxycycline. translation: MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPADALDDFDLDMLPADALDDFDLDMLPG"
     CDS             2386..3132
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPADALDDFDLDMLPADALDDFDLDMLPG*"
     primer_bind     complement(3172..3192)
                     /label="WPRE-R"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="note: WPRE, reverse primer"
     CDS             complement(3590..3601)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="product: Factor Xa recognition and cleavage site translation: IEGR"
     CDS             complement(3590..3601)
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="IEGR"
     LTR             3806..4039
                     /label="3' LTR (Delta-U3)"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="note: self-inactivating 3' long terminal repeat (LTR) fromHIV-1"
     polyA_signal    4111..4232
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="note: SV40 polyadenylation signal"
     primer_bind     complement(4148..4167)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="note: SV40 polyA, reverse primer"
     primer_bind     4202..4221
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="note: SV40 polyA terminator, reverse primer"
     rep_origin      4272..4407
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="note: SV40 origin of replication"
     primer_bind     4334..4353
                     /label="SV40pro-F"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: SV40 promoter/origin, forward primer"
     promoter        4407..4478
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="gene: bla"
     CDS             4479..5339
                     /label="AmpR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="gene: bla product: beta-lactamase note: confers resistance to ampicillin, carbenicillin, andrelated antibiotics translation: MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     CDS             4479..5339
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     primer_bind     complement(4697..4716)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="note: Ampicillin resistance gene, reverse primer"
     rep_origin      5510..6098
                     /label="ori"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     primer_bind     5999..6018
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: pBR322 origin, forward primer"
     primer_bind     6252..6269
                     /label="L4440"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="note: L4440 vector, forward primer"
     protein_bind    6386..6407
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     promoter        6422..6452
                     /label="lac promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    6460..6476
                     /label="lac operator"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6465..6487
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: In lacZ gene"
     primer_bind     6484..6500
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: In lacZ gene. Also called M13-rev"
     primer_bind     6484..6500
                     /label="M13 rev"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: common sequencing primer, one of multiple similar variants"
     primer_bind     6519..6539
                     /label="T3"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: T3 promoter, forward primer"
     promoter        6521..6539
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="note: promoter for bacteriophage T3 RNA polymerase"
     promoter        6567..6793
                     /label="RSV promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: Rous sarcoma virus enhancer/promoter"
     LTR             6794..6974
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7021..7146
                     /label="HIV-1 Psi"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: packaging signal of human immunodeficiency virus type 1"
     misc_feature    7639..7872
                     /label="RRE"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm."
     CDS             8057..8101
                     /label="gp41 peptide"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="product: antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013) note: recognized by the 2H10 single-chain llama nanobody translation: KNEQELLELDKWASL"
     CDS             8057..8101
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatgaca ttgattattg actagttatt aatagtaatc
      241 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      301 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      361 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg
      421 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      481 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      541 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      601 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      661 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      721 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      781 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac
      841 gactcactat agggagaccc aagcttGGTA CCACCGGTGC CACCATGGCC CACCACCACC
      901 AAGGCCCGCA GAGGGGCATA AGGGCCACAG CAGGGGTCTT GTGCTTCCTG GGTACAAGTA
      961 CCTCGGACCC TTCAACGGAC ACCAGCAGGG AGAGCCGGTC AACGAGGCAG ACGCCGCGGC
     1021 CGCTGAGCAT GAGCAAGCCT ACGACCGGCA GCCCGACAGC GGATAACACC CGTACCTCAA
     1081 GTGCGACCAG CCCGACGCGG AGTCTGGGCG CGCCGAAAGG CTGCTATGTG TTTCGCGGCC
     1141 AGACCCGCAC CAGCAGCCTG CCGGGCGAAA AAGAAGGCAG CTAAcctagg ctgcagacgc
     1201 gttgtacagg atccggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag
     1261 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
     1321 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
     1381 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
     1441 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
     1501 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
     1561 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
     1621 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
     1681 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccagcaatt caccatgacc
     1741 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     1801 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     1861 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     1921 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     1981 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     2041 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     2101 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     2161 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     2221 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     2281 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc cgaaggtaga
     2341 ggttctctcc tcacttgtgg tgatgttgaa gaaaaccctg gtccaatgtc tagactggac
     2401 aagagcaaag tcataaacgg agctctggaa ttactcaatg gtgtcggtat cgaaggcctg
     2461 acgacaagga aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg
     2521 aagaacaagc gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc
     2581 cacttctgcc ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca
     2641 taccgctgtg ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca
     2701 acagagaaac agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc
     2761 tccctggaga acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta
     2821 ttggaggaac aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct
     2881 atgcccccac ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc
     2941 ttccttttcg gcctggaact aatcatatgt ggcctggaga aacagctaaa gtgcgaaagc
     3001 ggcgggccga ccgacgccct tgacgatttt gacttagaca tgctcccagc cgatgccctt
     3061 gacgactttg accttgatat gctgcctgct gacgctcttg acgattttga ccttgacatg
     3121 ctccccgggt aactaagtaa ggatcgatcc aagatatcgt attcttaact atgttgctcc
     3181 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3241 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3301 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3361 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3421 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3481 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     3541 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3601 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3661 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg tttcgcctcg
     3721 gcgtccggac tagaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     3781 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     3841 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3901 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3961 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     4021 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     4081 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     4141 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4201 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     4261 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4321 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4381 agtagtgagg aggctttttt ggaggccttt caaatatgta tccgctcatg agacaataac
     4441 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4501 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4561 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4621 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4681 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4741 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4801 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4861 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4921 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4981 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5041 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5101 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5161 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5221 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5281 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5341 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5401 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5461 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5521 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5581 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5641 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5701 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5761 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5821 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5881 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5941 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6001 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6061 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6121 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6181 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6241 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     6301 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     6361 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     6421 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     6481 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     6541 aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt agtcttgcaa
     6601 catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc gtgcatgccg
     6661 attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga cgggtctgac
     6721 atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta agtgcctagc
     6781 tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6841 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6901 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6961 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     7021 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     7081 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     7141 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     7201 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     7261 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     7321 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     7381 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     7441 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     7501 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     7561 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     7621 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     7681 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     7741 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     7801 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7861 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7921 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7981 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     8041 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     8101 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     8161 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     8221 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gac
//