LOCUS       In-Vivo\Anneal         10603 bp    DNA     circular     20-Sep-2023
FEATURES             Location/Qualifiers
     terminator      5775..6014
                     /label=AgTEF terminator
                     /note=terminator
     promoter        2582..2610
                     /label=AmpR_promoter
                     /note=/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*
     CDS             2582..3512
                     /label=bla
                     /note=CDS
     CDS             5394..5774
                     /label=ble
                     /note=CDS
     misc_feature    complement(4297..4332)
                     /label=Gibson Assembly arm
                     /note=misc_feature
     misc_feature    4979..5008
                     /label=I-SceI
                     /note=misc_feature
     misc_feature    6039..6068
                     /label=I-SceI
                     /note=misc_feature
     misc_feature    4987..5004
                     /label=I-SceI site
                     /note=misc_feature
     misc_feature    6047..6064
                     /label=I-SceI site
                     /note=misc_feature
     misc_feature    2059..2202
                     /label=lacZ_a
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        complement(2057..2073)
                     /label=M13_forward20_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    complement(2066..2088)
                     /label=M13_pUC_fwd_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    4638..4660
                     /label=M13_pUC_rev_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        4659..4677
                     /label=M13_reverse_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    2401..2423
                     /label=pGEX_3_primer
                     /note=misc_feature
     misc_feature    4691..4840
                     /label=FCY1(207, 356)
                     /note=misc_feature
     misc_feature    1838..2052
                     /label=FCY1(454, 668)
                     /note=misc_feature
     misc_feature    4215..4287
                     /label=ori
                     /note=origin of replication
     misc_feature    complement(4194..4288)
                     /label=ori
                     /note=/SECDrawAs=Region
     CDS             3349..3357
                     /label=Linker(CDS)
                     /note=CDS
     CDS             554..562
                     /label=Linker(CDS)(CDS)
                     /note=CDS
     CDS             7800..7808
                     /label=Linker(CDS)(CDS)
                     /note=CDS
     misc_feature    3683..4271
                     /label=rep
                     /note=misc_feature
     promoter        2547..2651
                     /label=AmpR promoter
                     /note=promoter
     CDS             2652..3512
                     /label=AmpR
                     /note=confers resistance to ampicillin, carbenicillin, andrelated antibiotics
     protein_bind    4559..4580
                     /label=CAP binding site
                     /note=CAP binding activates transcription in the presenceof cAMP.
     promoter        4595..4625
                     /label=lac promoter
                     /note=promoter for the E. coli lac operon
     protein_bind    4633..4649
                     /label=lac operator
                     /note=The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
     primer_bind     4657..4673
                     /label=M13 rev
                     /note=common sequencing primer, one of multiple similar variants
     CDS             7800..7808
                     /label=Linker(CDS)(CDS)
                     /note=CDS
     CDS             554..562
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     CDS             3349..3357
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     misc_feature    4893..4932
                     /label=A
                     /note=misc_feature
     misc_feature    6069..6108
                     /label=A
                     /note=misc_feature
     misc_feature    4939..4978
                     /label=B
                     /note=misc_feature
     misc_feature    6115..6154
                     /label=B
                     /note=misc_feature
     misc_feature    5050..5393
                     /label=TEFp"/product="TEF1 promoter of Ashbya gossypii
                     /note=misc_feature
     misc_feature    5783..5977
                     /label=TEFt"/product="TEF1 terminator of Ashbya gossypii
                     /note=misc_feature
     promoter        6167..6926
                     /label=NRG2 promoter
                     /note=promoter
     CDS             6938..9580
                     /label=GAL4
                     /note=CDS
     promoter        9958..21
                     /label=ENO2 promoter
                     /note=promoter
     terminator      5775..5997
                     /label=AgTEF1 terminator
                     /note=terminator
     misc_feature    complement(2652..3512)
                     /label=bla
                     /note=misc_feature
     rep_origin      complement(4215..4287)
                     /label=AAG34515.1
                     /note=rep_origin
     misc_feature    5779..6014
                     /label=tAgTEF misc_feature
                     /note=misc_feature
     CDS             554..562
                     /label=Linker(CDS)(CDS)
                     /note=CDS
     CDS             3349..3357
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     CDS             7800..7808
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     CDS             34..1590
                     /label=GAL3 S509P
                     /note=CDS
     terminator      1594..1824
                     /label=ENO2 terminator
                     /note=terminator
     terminator      1594..1824
                     /label=ENO2 terminator
                     /note=terminator
     terminator      1594..1824
                     /label=ENO2 terminator
                     /note=terminator
     Primer          4691..4712
                     /label=PFCY1ups
                     /note=Primer
     Primer          complement(4824..4848)
                     /label=PFCY1upa
                     /note=Primer
     Primer          4855..4874
                     /label=PFCY1bles
                     /note=Primer
     Primer          1838..1861
                     /label=PFCY1downs
                     /note=Primer
     Primer          complement(2028..2052)
                     /label=PFCY1downa
                     /note=Primer
     Primer          6167..6192
                     /label=PGAL4NRG2ps
                     /note=Primer
     Primer          complement(6898..6926)
                     /label=PGAL4NRG2pa
                     /note=Primer
     Primer          6935..6960
                     /label=PGAL4GAL4s
                     /note=Primer
     Primer          complement(9925..9950)
                     /label=PGAL4GAL4a
                     /note=Primer
     Primer          9958..9983
                     /label=PQSENO2ps
                     /note=Primer
     Primer          complement(10595..21)
                     /label=PQSENO2pa
                     /note=Primer
     Primer          34..60
                     /label=PQSGAL3s
                     /note=Primer
     Primer          1594..1623
                     /label=F
                     /note=Primer
     Primer          complement(1800..1827)
                     /label=R
                     /note=Primer
     Primer          1..60
                     /label=PENO2pGAL3s
                     /note=Primer
     Primer          complement(1534..1593)
                     /label=PIRGAL3362a
                     /note=Primer
     Primer          1561..1620
                     /label=PQENO2ts
                     /note=Primer
     Primer          complement(1800..1853)
                     /label=PQENO2ta
                     /note=Primer
BASE COUNT     2998 a      2409 c      2229 g      2967 t 
ORIGIN
        1 ATACTATAAC ATACAATAAT AGGTACCAAA ATGAATACAA ACGTTCCAAT ATTCAGTTCT 
       61 CCGGTCAGAG ATTTACCAAG GTCTTTCGAA CAAAAACATT TAGCGGTTGT AGATGCATTT 
      121 TTCCAAACAT ACCATGTCAA ACCTGATTTT ATCGCTAGGT CTCCTGGCAG AGTAAATCTG 
      181 ATTGGTGAGC ATATAGATTA TTGCGATTTT TCAGTTTTGC CATTAGCCAT TGATGTGGAT 
      241 ATGCTTTGCG CAGTTAAAAT TTTAGACGAA AAAAATCCAT CCATTACCTT AACAAATGCG 
      301 GACCCTAAAT TTGCTCAGCG AAAGTTTGAT CTGCCTTTAG ATGGTTCCTA CATGGCCATA 
      361 GATCCGTCTG TGTCGGAATG GTCGAATTAC TTTAAATGCG GACTACATGT GGCACATTCA 
      421 TACTTGAAAA AAATTGCTCC GGAAAGATTT AATAATACAC CCTTAGTAGG TGCGCAGATC 
      481 TTTTGCCAGA GCGATATTCC TACTGGTGGT GGACTCTCAT CTGCATTTAC TTGCGCGGCA 
      541 GCACTAGCCA CAATTAGAGC CAATATGGGA AAAAATTTTG ATATTTCCAA AAAAGACTTG 
      601 ACCCGCATCA CAGCGGTTGC TGAGCACTAT GTTGGAGTCA ATAATGGTGG TATGGATCAA 
      661 GCAACGTCTG TTTATGGGGA AGAAGATCAT GCTCTATACG TAGAGTTTAG GCCAAAACTA 
      721 AAGGCCACAC CTTTCAAGTT TCCTCAATTG AAAAATCATG AAATCAGTTT CGTCATCGCC 
      781 AATACTCTTG TAAAGTCTAA TAAATTCGAA ACTGCTCCTA CAAATTACAA TTTAAGAGTA 
      841 ATAGAGGTAA CAGTTGCTGC CAACGCCTTG GCGACCAGAT ACAGCGTGGC CTTACCATCT 
      901 CACAAGGACA ATTCTAACTC AGAAAGAGGG AATCTAAGAG ATTTTATGGA TGCTTACTAC 
      961 GCCAGATACG AAAACCAAGC CCAACCATGG AATGGAGATA TCGGAACTGG TATTGAACGT 
     1021 TTACTCAAGA TGCTACAATT GGTAGAAGAA TCTTTCTCGA GGAAAAAGAG CGGATTCACT 
     1081 GTACATGAAG CCTCTACGGC GCTAAACTGT TCACGTGAGG AGTTCACTAG AGATTACCTG 
     1141 ACAACTTTTC CCGTCCGCTT CCAAGTCTTG AAACTATATC AAAGAGCTAA ACACGTTTAC 
     1201 TCCGAATCCT TAAGGGTGCT TAAGGCTTTA AAAATGATGA CAAGTGCCAC TTTTCACACG 
     1261 GACGAAGATT TCTTTACAGA TTTTGGCCGA CTAATGAATG AGTCCCAGGC CTCTTGTGAT 
     1321 AAACTTTATG AATGTTCGTG CATAGAAACC AATCAAATAT GCTCGATTGC CCTAGCAAAT 
     1381 GGTTCTTTCG GCTCCCGTCT CACTGGCGCT GGTTGGGGCG GTTGCACTAT CCACCTTGTT 
     1441 CCTAGTGGCG CTAATGGGAA CGTGGAACAG GTACGAAAAG CACTAATCGA AAAATTCTAC 
     1501 AATGTAAGAT ATCCGGATCT CACAGATGAA GAACTAAAAG ACGCAATTAT AGTTCCAAAG 
     1561 CCTGCCTTGG GTACTTGTTT GTACGAACAA TAAAGTGCTT TTAACTAAGA ATTATTAGTC 
     1621 TTTTCTGCTT ATTTTTTCAT CATAGTTTAG AACACTTTAT ATTAACGAAT AGTTTATGAA 
     1681 TCTATTTAGG TTTAAAAATT GATACAGTTT TATAAGTTAC TTTTTCAAAG ACTCGTGCTG 
     1741 TCTATTGCAT AATGCACTGG AAGGGGAAAA AAAAGGTGCA CACGCGTGGC TTTTTCTTGA 
     1801 ATTTGCAGTT TGAAAAATAA CTACATGTAA GGCCTATGGA TTGGTTTGAA GATATTGGTG 
     1861 AGTAGAGCAC GCAGCACGCT GTATTTACGT ATTTAATTTT ATATATTTGT GCATACACTA 
     1921 CTAGGGAAGA CTTGAAAAAA ACCTAGGAAA TGAAAAAACG ACACAGGAAG TCCCGTATTT 
     1981 ACTATTTTTT CCTTCCTTTT GATGGGGCAG GGCGGAAATA GAGGATAGGA TAAGCCTACT 
     2041 GCTTAGCTGT TTAAACACTG GCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG 
     2101 TTACCCAACT TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG 
     2161 AGGCCCGCAC CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCCTGA 
     2221 TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATGG TGCACTCTCA 
     2281 GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG 
     2341 ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT 
     2401 CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGACGAAAGG 
     2461 GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT 
     2521 CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC 
     2581 ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA 
     2641 AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT 
     2701 TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC 
     2761 AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA 
     2821 GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG 
     2881 CGGTATTATC CCGTATTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC 
     2941 AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG 
     3001 TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC 
     3061 TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG 
     3121 TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG 
     3181 ACACCACGAT GCCTGTAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC 
     3241 TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC 
     3301 CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG 
     3361 AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG 
     3421 TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG 
     3481 AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC 
     3541 TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG 
     3601 ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG 
     3661 TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC 
     3721 AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC 
     3781 TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTT CTTCTAGTGT 
     3841 AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC 
     3901 TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT 
     3961 CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC 
     4021 AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG 
     4081 AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG 
     4141 GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG 
     4201 TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA 
     4261 GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT 
     4321 TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT 
     4381 TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG 
     4441 AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT 
     4501 AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA 
     4561 ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA 
     4621 TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT 
     4681 ACGCCAGTTT AAACTGTGGG AGATTAGAGG GCAAAGTGTA CAAAGATACC ACTTTGTATA 
     4741 CGACGCTGTC TCCATGCGAC ATGTGTACAG GTGCCATCAT CATGTATGGT ATTCCACGCT 
     4801 GTGTTGTCGG TGAGAACGTT AATTTCAAAA GTAAGGGCGA TTAATTAAGG ATCCGCTGAA 
     4861 GCTTCGTACG CTGCAGGTCG ACAACCCTTA ATGTTCTTCA TTCTACGCAC GGTCCTTCAA 
     4921 TTCGCTTGGC GGAGATCTAT GACAAGAGGG TCGAACTCGC CTAAGTCGTA ATTGAGTCAG 
     4981 TTACGCTAGG GATAACAGGG TAATATAGCT GTTTAGCTTG CCTCGTCCCC GCCGGGTCAC 
     5041 CCGGCCAGCG ACATGGAGGC CCAGAATACC CTCCTTGACA GTCTTGACGT GCGCAGCTCA 
     5101 GGGGCATGAT GTGACTGTCG CCCGTACATT TAGCCCATAC ATCCCCATGT ATAATCATTT 
     5161 GCATCCATAC ATTTTGATGG CCGCACGGCG CGAAGCAAAA ATTACGGCTC CTCGCTGCAG 
     5221 ACCTGCGAGC AGGGAAACGC TCCCCTCACA GACGCGTTGA ATTGTCCCCA CGCCGCGCCC 
     5281 CTGTAGAGAA ATATAAAAGG TTAGGATTTG CCACTGAGGT TCTTCTTTCA TATACTTCCT 
     5341 TTTAAAATCT TGCTAGGATA CAGTTCTCAC ATCACATCCG AACATAAACA ACCATGGCCG 
     5401 ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACGGCCGCC TTCTATGAAA 
     5461 GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC 
     5521 TCAAGCTGGA GTTCTTCGCC CACCCCGGGC TCGATCCCCT CGCGAGTTGG TTCAGCTGCT 
     5581 GCCTGAGGCT GGACGACCTC GCGGAGTTCT ACCGGCAGTG CAAATCCGTC GGCATCCAGG 
     5641 AAACCAGCAG CGGCTATCCG CGCATCCATG CCCCCGAACT GCAGGAGTGG GGAGGCACGA 
     5701 TGGCCGCTTT GGTCGACCCG GACGGGACGC TCCTGCGCCT GATACAGAAC GAATTGCTTG 
     5761 CAGGCATCTC ATGATCAGTA CTGACAATAA AAAGATTCTT GTTTTCAAGA ACTTGTCATT 
     5821 TGTATAGTTT TTTTATATTG TAGTTGTTCT ATTTTAATCA AATGTTAGCG TGATTTATAT 
     5881 TTTTTTTCGC CTCGACATCA TCTGCCCAGA TGCGAAGTTA AGTGCGCAGA AAGTAATATC 
     5941 ATGCGTCAAT CGTATGTGAA TGCTGGTCGC TATACTGCTG TCGATTCGAT ACTAACGCCG 
     6001 CCATCCAGTG TCGAAAACGA GCTCTCGAGA ACCCTTAAAG TTACGCTAGG GATAACAGGG 
     6061 TAATATAGGT TCTTCATTCT ACGCACGGTC CTTCAATTCG CTTGGCGGTC TAGAATGACA 
     6121 AGAGGGTCGA ACTCGCCTAA GTCGTAATTG AGTCCAGATC CACCGCGGCT CTGCATATAG 
     6181 AATAACAAAC AAATCGTATT TTTCCCGAAG CCAAGAAAAA AAGGAGACTA TAACCTTTCA 
     6241 AGCCAGACGG AAAACTATCT GCCCAAGGAC AAACTGGCAT GGGCTGCTTC AATGTTCGGC 
     6301 TACCTTTTTT CTCTTTTAAC GGGGTTTGGT ATTTTTTTTC TTTACTTCAT AAACGAATGT 
     6361 TAAACGACTG TGAAAAAGCC ATCCCTGGAT TATGCGAGAG TACTCTCAGG GCACCCGTCC 
     6421 GTTTAAAGAC CCCAGGCCAA AATAAAAATT TCTTGAAGGA AAAAAATACA CAGGGCCTTG 
     6481 AACTGAATAC AAAGCCATCT TTTTTCTCGT TTGTCAGCAC GTCCCAGAAA AACGCGCAGC 
     6541 CGTAGCTCTT GATAAGTGGC TTCTGCGTTT TGCAACGCTT TTCTGATCTC ATGTTTTCTT 
     6601 ATGCTGCGGG ACATCATTGT CGTTGAGAAA AGTTTCAATT TCAAACGAAC TGCCTACCGT 
     6661 TGCATTCTCC TTGGAGAAGG GCCTGAGTTT AGGGGAATCA CGTCTGGCTA TTACATGCTA 
     6721 CATCGTGCAG TAGCTCCTAC ATCGCTAACA GGCAAATCCT ACACCCTCTT TAAATCGTTT 
     6781 ATCTTTTATA TATTATCTGG CCTAAATTTT TACATATAAA TATTTATCAT ATTATGACAG 
     6841 CTCAAATGAA TTTCCGGCAC CAAGTCATAT GAGCACTCGC TACTGTTCGA AGAACAAAAA 
     6901 ATATAAAGCT TCTACAAAAT TTCCAGAATT CACAATGAAG CTACTGTCTT CTATCGAACA 
     6961 AGCATGCGAT ATTTGCCGAC TTAAAAAGCT CAAGTGCTCC AAAGAAAAAC CGAAGTGCGC 
     7021 CAAGTGTCTG AAGAACAACT GGGAGTGTCG CTACTCTCCC AAAACCAAAA GGTCTCCGCT 
     7081 GACTAGGGCA CATCTGACAG AAGTGGAATC AAGGCTAGAA AGACTGGAAC AGCTATTTCT 
     7141 ACTGATTTTT CCTCGAGAAG ACCTTGACAT GATTTTGAAA ATGGATTCTT TACAGGATAT 
     7201 AAAAGCATTG TTAACAGGAT TATTTGTACA AGATAATGTG AATAAAGATG CCGTCACAGA 
     7261 TAGATTGGCT TCAGTGGAGA CTGATATGCC TCTAACATTG AGACAGCATA GAATAAGTGC 
     7321 GACATCATCA TCGGAAGAGA GTAGTAACAA AGGTCAAAGA CAGTTGACTG TATCGATTGA 
     7381 CTCGGCAGCT CATCATGATA ACTCCACAAT TCCGTTGGAT TTTATGCCCA GGGATGCTCT 
     7441 TCATGGATTT GATTGGTCTG AAGAGGATGA CATGTCGGAT GGCTTGCCCT TCCTGAAAAC 
     7501 GGACCCCAAC AATAATGGGT TCTTTGGCGA CGGTTCTCTC TTATGTATTC TTCGATCTAT 
     7561 TGGCTTTAAA CCGGAAAATT ACACGAACTC TAACGTTAAC AGGCTCCCGA CCATGATTAC 
     7621 GGATAGATAC ACGTTGGCTT CTAGATCCAC AACATCCCGT TTACTTCAAA GTTATCTCAA 
     7681 TAATTTTCAC CCCTACTGCC CTATCGTGCA CTCACCGACG CTAATGATGT TGTATAATAA 
     7741 CCAGATTGAA ATCGCGTCGA AGGATCAATG GCAAATCCTT TTTAACTGCA TATTAGCCAT 
     7801 TGGAGCCTGG TGTATAGAGG GGGAATCTAC TGATATAGAT GTTTTTTACT ATCAAAATGC 
     7861 TAAATCTCAT TTGACGAGCA AGGTCTTCGA GTCAGGTTCC ATAATTTTGG TGACAGCCCT 
     7921 ACATCTTCTG TCGCGATATA CACAGTGGAG GCAGAAAACA AATACTAGCT ATAATTTTCA 
     7981 CAGCTTTTCC ATAAGAATGG CCATATCATT GGGCTTGAAT AGGGACCTCC CCTCGTCCTT 
     8041 CAGTGATAGC AGCATTCTGG AACAAAGACG CCGAATTTGG TGGTCTGTCT ACTCTTGGGA 
     8101 GATCCAATTG TCCCTGCTTT ATGGTCGATC CATCCAGCTT TCTCAGAATA CAATCTCCTT 
     8161 CCCTTCTTCT GTCGACGATG TGCAGCGTAC CACAACAGGT CCCACCATAT ATCATGGCAT 
     8221 CATTGAAACA GCAAGGCTCT TACAAGTTTT CACAAAAATC TATGAACTAG ACAAAACAGT 
     8281 AACTGCAGAA AAAAGTCCTA TATGTGCAAA AAAATGCTTG ATGATTTGTA ATGAGATTGA 
     8341 GGAGGTTTCG AGACAGGCAC CAAAGTTTTT ACAAATGGAT ATTTCCACCA CCGCTCTAAC 
     8401 CAATTTGTTG AAGGAACACC CTTGGCTATC CTTTACAAGA TTCGAACTGA AGTGGAAACA 
     8461 GTTGTCTCTT ATCATTTATG TATTAAGAGA TTTTTTCACT AATTTTACCC AGAAAAAGTC 
     8521 ACAACTAGAA CAGGATCAAA ATGATCATCA AAGTTATGAA GTTAAACGAT GCTCCATCAT 
     8581 GTTAAGCGAT GCAGCACAAA GAACTGTTAT GTCTGTAAGT AGCTATATGG ACAATCATAA 
     8641 TGTCACCCCA TATTTTGCCT GGAATTGTTC TTATTACTTG TTCAATGCAG TCCTAGTACC 
     8701 CATAAAGACT CTACTCTCAA ACTCAAAATC GAATGCTGAG AATAACGAGA CCGCACAATT 
     8761 ATTACAACAA ATTAACACTG TTCTGATGCT ATTAAAAAAA CTGGCCACTT TTAAAATCCA 
     8821 GACTTGTGAA AAATACATTC AAGTACTGGA AGAGGTATGT GCGCCGTTTC TGTTATCACA 
     8881 GTGTGCAATC CCATTACCGC ATATCAGTTA TAACAATAGT AATGGTAGCG CCATTAAAAA 
     8941 TATTGTCGGT TCTGCAACTA TCGCCCAATA CCCTACTCTT CCGGAGGAAA ATGTCAACAA 
     9001 TATCAGTGTT AAATATGTTT CTCCTGGCTC AGTAGGGCCT TCACCTGTGC CATTGAAATC 
     9061 AGGAGCAAGT TTCAGTGATC TAGTCAAGCT GTTATCTAAC CGTCCACCCT CTCGTAACTC 
     9121 TCCAGTGACA ATACCAAGAA GCACACCTTC GCATCGCTCA GTCACGCCTT TTCTAGGGCA 
     9181 ACAGCAACAG CTGCAATCAT TAGTGCCACT GACCCCGTCT GCTTTGTTTG GTGGCGCCAA 
     9241 TTTTAATCAA AGTGGGAATA TTGCTGATAG CTCATTGTCC TTCACTTTCA CTAACAGTAG 
     9301 CAACGGTCCG AACCTCATAA CAACTCAAAC AAATTCTCAA GCGCTTTCAC AACCAATTGC 
     9361 CTCCTCTAAC GTTCATGATA ACTTCATGAA TAATGAAATC ACGGCTAGTA AAATTGATGA 
     9421 TGGTAATAAT TCAAAACCAC TGTCACCTGG TTGGACGGAC CAAACTGCGT ATAACGCGTT 
     9481 TGGAATCACT ACAGGGATGT TTAATACCAC TACAATGGAT GATGTATATA ACTATCTATT 
     9541 CGATGATGAA GATACCCCAC CAAACCCAAA AAAAGAGTAA AATGAATCGT AGATACTGAA 
     9601 AAACCCCGCA AGTTCACTTC AACTGTGCAT CGTGCACCAT CTCAATTTCT TTCATTTATA 
     9661 CATCGTTTTG CCTTCTTTTA TGTAACTATA CTCCTCTAAG TTTCAATCTT GGCCATGTAA 
     9721 CCTCTGATCT ATAGAATTTT TTAAATGACT AGAATTAATG CCCATCTTTT TTTTGGACCT 
     9781 AAATTCTTCA TGAAAATATA TTACGAGGGC TTATTCAGAA GCTTCGCTCA TATAACGAAA 
     9841 AAAAAGGGTT TGGATCGAAC GTAATTGAGA TTGATTAGTT AATACTCAAA ATAAAACAGC 
     9901 TCCTACCACC AGTGTAAAGT AGAACGTTAA TAGAGCAATG TCTTCAGACA GCGGCCGCGG 
     9961 AAGTGTCTCA TAAACTTTAC TCAAGAGTTA AGTACTGAAA AATTCGACTT TTATGATAGT 
    10021 TCAAGTGTCG ACGCTGCGGG TATAGAAAGG GTTCTTTACT CTATAGTACC TCCTCGCTCA 
    10081 GCATCTGCTT CTTCCCAAAG ATGAACGCGG CGTTATGTCA CTAACGACGT GCACCAACTT 
    10141 GCGGAAAGTG GAATCCCGTT CCAAAACTGG CATCCACTAA TTGATACATC TACACACCGC 
    10201 ACGCCTTTTT TCTGAAGCCC ACTTTCGTGG ACTTTGCCAT ATGCAAAATT CATGAAGTGT 
    10261 GATACCAAGT CAGCATACAC CTCACTAGGG TAGTTTCTTT GGTTGTATTG ATCATTTGGT 
    10321 TCATCGTGGT TCATTAATTT TTTTTCTCCA TTGCTTTCTG GCTTTGATCT TACTATCATT 
    10381 TGGATTTTTG TCGAAGGTTG TAGAATTGTA TGTGACAAGT GGCACCAAGC ATATATAAAA 
    10441 AAAAAAAGCA TTATCTTCCT ACCAGAGTTG ATTGTTAAAA ACGTATTTAT AGCAAACGCA 
    10501 ATTGTAATTA ATTCTTATTT TGTATCTTTT CTTCCCTTGT CTCAATCTTT TATTTTTATT 
    10561 TTATTTTTCT TTTCTTAGTT TCTTTCATAA CACCAAGCAA CTA 
//