LOCUS       In-Vivo\Anneal         11425 bp    DNA     circular     20-Sep-2023
FEATURES             Location/Qualifiers
     CDS             complement(9607..9624)
                     /label=6*His tag(CDS)(CDS)
                     /note=CDS
     terminator      3943..4182
                     /label=AgTEF terminator
                     /note=terminator
     promoter        750..778
                     /label=AmpR_promoter
                     /note=/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*
     CDS             750..1680
                     /label=bla
                     /note=CDS
     CDS             3562..3942
                     /label=ble
                     /note=CDS
     misc_feature    complement(2465..2500)
                     /label=Gibson Assembly arm
                     /note=misc_feature
     CDS             9609..9626
                     /label=Gly6
                     /note=CDS
     CDS             9609..9626
                     /label=Gly6(CDS)(CDS)
                     /note=CDS
     misc_feature    3147..3176
                     /label=I-SceI
                     /note=misc_feature
     misc_feature    4207..4236
                     /label=I-SceI
                     /note=misc_feature
     misc_feature    3155..3172
                     /label=I-SceI site
                     /note=misc_feature
     misc_feature    4215..4232
                     /label=I-SceI site
                     /note=misc_feature
     misc_feature    227..370
                     /label=lacZ_a
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        complement(225..241)
                     /label=M13_forward20_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    complement(234..256)
                     /label=M13_pUC_fwd_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    2806..2828
                     /label=M13_pUC_rev_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        2827..2845
                     /label=M13_reverse_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    569..591
                     /label=pGEX_3_primer
                     /note=misc_feature
     misc_feature    2859..3008
                     /label=FCY1(207, 356)
                     /note=misc_feature
     misc_feature    6..220
                     /label=FCY1(454, 668)
                     /note=misc_feature
     misc_feature    2383..2455
                     /label=ori
                     /note=origin of replication
     misc_feature    complement(2362..2456)
                     /label=ori
                     /note=/SECDrawAs=Region
     CDS             1517..1525
                     /label=Linker(CDS)
                     /note=CDS
     CDS             5968..5976
                     /label=Linker(CDS)(CDS)
                     /note=CDS
     CDS             10147..10155
                     /label=Linker(CDS)(CDS)
                     /note=CDS
     misc_feature    1851..2439
                     /label=rep
                     /note=misc_feature
     promoter        715..819
                     /label=AmpR promoter
                     /note=promoter
     CDS             820..1680
                     /label=AmpR
                     /note=confers resistance to ampicillin, carbenicillin, andrelated antibiotics
     protein_bind    2727..2748
                     /label=CAP binding site
                     /note=CAP binding activates transcription in the presenceof cAMP.
     promoter        2763..2793
                     /label=lac promoter
                     /note=promoter for the E. coli lac operon
     protein_bind    2801..2817
                     /label=lac operator
                     /note=The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG).
     primer_bind     2825..2841
                     /label=M13 rev
                     /note=common sequencing primer, one of multiple similar variants
     CDS             5968..5976
                     /label=Linker(CDS)(CDS)
                     /note=CDS
     CDS             1517..1525
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     CDS             10147..10155
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     misc_feature    3061..3100
                     /label=A
                     /note=misc_feature
     misc_feature    4237..4276
                     /label=A
                     /note=misc_feature
     misc_feature    3107..3146
                     /label=B
                     /note=misc_feature
     misc_feature    4283..4322
                     /label=B
                     /note=misc_feature
     misc_feature    3218..3561
                     /label=TEFp"/product="TEF1 promoter of Ashbya gossypii
                     /note=misc_feature
     misc_feature    3951..4145
                     /label=TEFt"/product="TEF1 terminator of Ashbya gossypii
                     /note=misc_feature
     promoter        4335..5094
                     /label=NRG2 promoter
                     /note=promoter
     CDS             5106..7748
                     /label=GAL4
                     /note=CDS
     promoter        8126..8792
                     /label=ENO2 promoter
                     /note=promoter
     CDS             8802..9029
                     /label=UBI4
                     /note=CDS
     CDS             9030..9053
                     /label=N-degron linker
                     /note=CDS
     CDS             9054..9608
                     /label=DHFR
                     /note=CDS
     terminator      3943..4165
                     /label=AgTEF1 terminator
                     /note=terminator
     misc_feature    complement(820..1680)
                     /label=bla
                     /note=misc_feature
     rep_origin      complement(2383..2455)
                     /label=AAG34515.1
                     /note=rep_origin
     misc_feature    3947..4182
                     /label=tAgTEF misc_feature
                     /note=misc_feature
     misc_feature    9036..9044
                     /label=GSG linker
                     /note=misc_feature
     CDS             complement(9607..9624)
                     /label=6*His tag(CDS)(CDS)
                     /note=CDS
     CDS             10147..10155
                     /label=Linker(CDS)(CDS)
                     /note=CDS
     CDS             1517..1525
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     CDS             5968..5976
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     CDS             9627..11183
                     /label=GAL3 S509P
                     /note=CDS
     terminator      11187..11417
                     /label=ENO2 terminator
                     /note=terminator
     terminator      11187..11417
                     /label=ENO2 terminator
                     /note=terminator
     terminator      11187..11417
                     /label=ENO2 terminator
                     /note=terminator
     Primer          2859..2880
                     /label=PFCY1ups
                     /note=Primer
     Primer          complement(2992..3016)
                     /label=PFCY1upa
                     /note=Primer
     Primer          3023..3042
                     /label=PFCY1bles
                     /note=Primer
     Primer          6..29
                     /label=PFCY1downs
                     /note=Primer
     Primer          complement(196..220)
                     /label=PFCY1downa
                     /note=Primer
     Primer          4335..4360
                     /label=PGAL4NRG2ps
                     /note=Primer
     Primer          complement(5066..5094)
                     /label=PGAL4NRG2pa
                     /note=Primer
     Primer          5103..5128
                     /label=PGAL4GAL4s
                     /note=Primer
     Primer          complement(8093..8118)
                     /label=PGAL4GAL4a
                     /note=Primer
     Primer          8126..8151
                     /label=PQSENO2ps
                     /note=Primer
     Primer          complement(8763..8792)
                     /label=PQSENO2pa
                     /note=Primer
     Primer          8802..8825
                     /label=PQSHDs
                     /note=Primer
     Primer          complement(9606..9626)
                     /label=PQSHDa
                     /note=Primer
     Primer          9627..9653
                     /label=PQSGAL3s
                     /note=Primer
     Primer          11187..11216
                     /label=F
                     /note=Primer
     Primer          complement(11393..11420)
                     /label=R
                     /note=Primer
     Primer          complement(11127..11186)
                     /label=PIRGAL3362a
                     /note=Primer
     Primer          11154..11213
                     /label=PQENO2ts
                     /note=Primer
     Primer          complement(11393..21)
                     /label=PQENO2ta
                     /note=Primer
BASE COUNT     3246 a      2553 c      2428 g      3198 t 
ORIGIN
        1 CCTATGGATT GGTTTGAAGA TATTGGTGAG TAGAGCACGC AGCACGCTGT ATTTACGTAT 
       61 TTAATTTTAT ATATTTGTGC ATACACTACT AGGGAAGACT TGAAAAAAAC CTAGGAAATG 
      121 AAAAAACGAC ACAGGAAGTC CCGTATTTAC TATTTTTTCC TTCCTTTTGA TGGGGCAGGG 
      181 CGGAAATAGA GGATAGGATA AGCCTACTGC TTAGCTGTTT AAACACTGGC CGTCGTTTTA 
      241 CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC 
      301 CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG 
      361 CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT 
      421 ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC 
      481 CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA 
      541 TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG 
      601 TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 
      661 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 
      721 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 
      781 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 
      841 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 
      901 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 
      961 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 
     1021 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 
     1081 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 
     1141 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 
     1201 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 
     1261 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 
     1321 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 
     1381 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 
     1441 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 
     1501 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 
     1561 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 
     1621 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 
     1681 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 
     1741 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 
     1801 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 
     1861 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 
     1921 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 
     1981 CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 
     2041 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 
     2101 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 
     2161 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 
     2221 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 
     2281 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 
     2341 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 
     2401 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 
     2461 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 
     2521 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 
     2581 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 
     2641 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 
     2701 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 
     2761 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 
     2821 CACACAGGAA ACAGCTATGA CCATGATTAC GCCAGTTTAA ACTGTGGGAG ATTAGAGGGC 
     2881 AAAGTGTACA AAGATACCAC TTTGTATACG ACGCTGTCTC CATGCGACAT GTGTACAGGT 
     2941 GCCATCATCA TGTATGGTAT TCCACGCTGT GTTGTCGGTG AGAACGTTAA TTTCAAAAGT 
     3001 AAGGGCGATT AATTAAGGAT CCGCTGAAGC TTCGTACGCT GCAGGTCGAC AACCCTTAAT 
     3061 GTTCTTCATT CTACGCACGG TCCTTCAATT CGCTTGGCGG AGATCTATGA CAAGAGGGTC 
     3121 GAACTCGCCT AAGTCGTAAT TGAGTCAGTT ACGCTAGGGA TAACAGGGTA ATATAGCTGT 
     3181 TTAGCTTGCC TCGTCCCCGC CGGGTCACCC GGCCAGCGAC ATGGAGGCCC AGAATACCCT 
     3241 CCTTGACAGT CTTGACGTGC GCAGCTCAGG GGCATGATGT GACTGTCGCC CGTACATTTA 
     3301 GCCCATACAT CCCCATGTAT AATCATTTGC ATCCATACAT TTTGATGGCC GCACGGCGCG 
     3361 AAGCAAAAAT TACGGCTCCT CGCTGCAGAC CTGCGAGCAG GGAAACGCTC CCCTCACAGA 
     3421 CGCGTTGAAT TGTCCCCACG CCGCGCCCCT GTAGAGAAAT ATAAAAGGTT AGGATTTGCC 
     3481 ACTGAGGTTC TTCTTTCATA TACTTCCTTT TAAAATCTTG CTAGGATACA GTTCTCACAT 
     3541 CACATCCGAA CATAAACAAC CATGGCCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT 
     3601 TTCGATTCCA CGGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC 
     3661 GGCTGGATGA TCCTCCAGCG CGGGGATCTC AAGCTGGAGT TCTTCGCCCA CCCCGGGCTC 
     3721 GATCCCCTCG CGAGTTGGTT CAGCTGCTGC CTGAGGCTGG ACGACCTCGC GGAGTTCTAC 
     3781 CGGCAGTGCA AATCCGTCGG CATCCAGGAA ACCAGCAGCG GCTATCCGCG CATCCATGCC 
     3841 CCCGAACTGC AGGAGTGGGG AGGCACGATG GCCGCTTTGG TCGACCCGGA CGGGACGCTC 
     3901 CTGCGCCTGA TACAGAACGA ATTGCTTGCA GGCATCTCAT GATCAGTACT GACAATAAAA 
     3961 AGATTCTTGT TTTCAAGAAC TTGTCATTTG TATAGTTTTT TTATATTGTA GTTGTTCTAT 
     4021 TTTAATCAAA TGTTAGCGTG ATTTATATTT TTTTTCGCCT CGACATCATC TGCCCAGATG 
     4081 CGAAGTTAAG TGCGCAGAAA GTAATATCAT GCGTCAATCG TATGTGAATG CTGGTCGCTA 
     4141 TACTGCTGTC GATTCGATAC TAACGCCGCC ATCCAGTGTC GAAAACGAGC TCTCGAGAAC 
     4201 CCTTAAAGTT ACGCTAGGGA TAACAGGGTA ATATAGGTTC TTCATTCTAC GCACGGTCCT 
     4261 TCAATTCGCT TGGCGGTCTA GAATGACAAG AGGGTCGAAC TCGCCTAAGT CGTAATTGAG 
     4321 TCCAGATCCA CCGCGGCTCT GCATATAGAA TAACAAACAA ATCGTATTTT TCCCGAAGCC 
     4381 AAGAAAAAAA GGAGACTATA ACCTTTCAAG CCAGACGGAA AACTATCTGC CCAAGGACAA 
     4441 ACTGGCATGG GCTGCTTCAA TGTTCGGCTA CCTTTTTTCT CTTTTAACGG GGTTTGGTAT 
     4501 TTTTTTTCTT TACTTCATAA ACGAATGTTA AACGACTGTG AAAAAGCCAT CCCTGGATTA 
     4561 TGCGAGAGTA CTCTCAGGGC ACCCGTCCGT TTAAAGACCC CAGGCCAAAA TAAAAATTTC 
     4621 TTGAAGGAAA AAAATACACA GGGCCTTGAA CTGAATACAA AGCCATCTTT TTTCTCGTTT 
     4681 GTCAGCACGT CCCAGAAAAA CGCGCAGCCG TAGCTCTTGA TAAGTGGCTT CTGCGTTTTG 
     4741 CAACGCTTTT CTGATCTCAT GTTTTCTTAT GCTGCGGGAC ATCATTGTCG TTGAGAAAAG 
     4801 TTTCAATTTC AAACGAACTG CCTACCGTTG CATTCTCCTT GGAGAAGGGC CTGAGTTTAG 
     4861 GGGAATCACG TCTGGCTATT ACATGCTACA TCGTGCAGTA GCTCCTACAT CGCTAACAGG 
     4921 CAAATCCTAC ACCCTCTTTA AATCGTTTAT CTTTTATATA TTATCTGGCC TAAATTTTTA 
     4981 CATATAAATA TTTATCATAT TATGACAGCT CAAATGAATT TCCGGCACCA AGTCATATGA 
     5041 GCACTCGCTA CTGTTCGAAG AACAAAAAAT ATAAAGCTTC TACAAAATTT CCAGAATTCA 
     5101 CAATGAAGCT ACTGTCTTCT ATCGAACAAG CATGCGATAT TTGCCGACTT AAAAAGCTCA 
     5161 AGTGCTCCAA AGAAAAACCG AAGTGCGCCA AGTGTCTGAA GAACAACTGG GAGTGTCGCT 
     5221 ACTCTCCCAA AACCAAAAGG TCTCCGCTGA CTAGGGCACA TCTGACAGAA GTGGAATCAA 
     5281 GGCTAGAAAG ACTGGAACAG CTATTTCTAC TGATTTTTCC TCGAGAAGAC CTTGACATGA 
     5341 TTTTGAAAAT GGATTCTTTA CAGGATATAA AAGCATTGTT AACAGGATTA TTTGTACAAG 
     5401 ATAATGTGAA TAAAGATGCC GTCACAGATA GATTGGCTTC AGTGGAGACT GATATGCCTC 
     5461 TAACATTGAG ACAGCATAGA ATAAGTGCGA CATCATCATC GGAAGAGAGT AGTAACAAAG 
     5521 GTCAAAGACA GTTGACTGTA TCGATTGACT CGGCAGCTCA TCATGATAAC TCCACAATTC 
     5581 CGTTGGATTT TATGCCCAGG GATGCTCTTC ATGGATTTGA TTGGTCTGAA GAGGATGACA 
     5641 TGTCGGATGG CTTGCCCTTC CTGAAAACGG ACCCCAACAA TAATGGGTTC TTTGGCGACG 
     5701 GTTCTCTCTT ATGTATTCTT CGATCTATTG GCTTTAAACC GGAAAATTAC ACGAACTCTA 
     5761 ACGTTAACAG GCTCCCGACC ATGATTACGG ATAGATACAC GTTGGCTTCT AGATCCACAA 
     5821 CATCCCGTTT ACTTCAAAGT TATCTCAATA ATTTTCACCC CTACTGCCCT ATCGTGCACT 
     5881 CACCGACGCT AATGATGTTG TATAATAACC AGATTGAAAT CGCGTCGAAG GATCAATGGC 
     5941 AAATCCTTTT TAACTGCATA TTAGCCATTG GAGCCTGGTG TATAGAGGGG GAATCTACTG 
     6001 ATATAGATGT TTTTTACTAT CAAAATGCTA AATCTCATTT GACGAGCAAG GTCTTCGAGT 
     6061 CAGGTTCCAT AATTTTGGTG ACAGCCCTAC ATCTTCTGTC GCGATATACA CAGTGGAGGC 
     6121 AGAAAACAAA TACTAGCTAT AATTTTCACA GCTTTTCCAT AAGAATGGCC ATATCATTGG 
     6181 GCTTGAATAG GGACCTCCCC TCGTCCTTCA GTGATAGCAG CATTCTGGAA CAAAGACGCC 
     6241 GAATTTGGTG GTCTGTCTAC TCTTGGGAGA TCCAATTGTC CCTGCTTTAT GGTCGATCCA 
     6301 TCCAGCTTTC TCAGAATACA ATCTCCTTCC CTTCTTCTGT CGACGATGTG CAGCGTACCA 
     6361 CAACAGGTCC CACCATATAT CATGGCATCA TTGAAACAGC AAGGCTCTTA CAAGTTTTCA 
     6421 CAAAAATCTA TGAACTAGAC AAAACAGTAA CTGCAGAAAA AAGTCCTATA TGTGCAAAAA 
     6481 AATGCTTGAT GATTTGTAAT GAGATTGAGG AGGTTTCGAG ACAGGCACCA AAGTTTTTAC 
     6541 AAATGGATAT TTCCACCACC GCTCTAACCA ATTTGTTGAA GGAACACCCT TGGCTATCCT 
     6601 TTACAAGATT CGAACTGAAG TGGAAACAGT TGTCTCTTAT CATTTATGTA TTAAGAGATT 
     6661 TTTTCACTAA TTTTACCCAG AAAAAGTCAC AACTAGAACA GGATCAAAAT GATCATCAAA 
     6721 GTTATGAAGT TAAACGATGC TCCATCATGT TAAGCGATGC AGCACAAAGA ACTGTTATGT 
     6781 CTGTAAGTAG CTATATGGAC AATCATAATG TCACCCCATA TTTTGCCTGG AATTGTTCTT 
     6841 ATTACTTGTT CAATGCAGTC CTAGTACCCA TAAAGACTCT ACTCTCAAAC TCAAAATCGA 
     6901 ATGCTGAGAA TAACGAGACC GCACAATTAT TACAACAAAT TAACACTGTT CTGATGCTAT 
     6961 TAAAAAAACT GGCCACTTTT AAAATCCAGA CTTGTGAAAA ATACATTCAA GTACTGGAAG 
     7021 AGGTATGTGC GCCGTTTCTG TTATCACAGT GTGCAATCCC ATTACCGCAT ATCAGTTATA 
     7081 ACAATAGTAA TGGTAGCGCC ATTAAAAATA TTGTCGGTTC TGCAACTATC GCCCAATACC 
     7141 CTACTCTTCC GGAGGAAAAT GTCAACAATA TCAGTGTTAA ATATGTTTCT CCTGGCTCAG 
     7201 TAGGGCCTTC ACCTGTGCCA TTGAAATCAG GAGCAAGTTT CAGTGATCTA GTCAAGCTGT 
     7261 TATCTAACCG TCCACCCTCT CGTAACTCTC CAGTGACAAT ACCAAGAAGC ACACCTTCGC 
     7321 ATCGCTCAGT CACGCCTTTT CTAGGGCAAC AGCAACAGCT GCAATCATTA GTGCCACTGA 
     7381 CCCCGTCTGC TTTGTTTGGT GGCGCCAATT TTAATCAAAG TGGGAATATT GCTGATAGCT 
     7441 CATTGTCCTT CACTTTCACT AACAGTAGCA ACGGTCCGAA CCTCATAACA ACTCAAACAA 
     7501 ATTCTCAAGC GCTTTCACAA CCAATTGCCT CCTCTAACGT TCATGATAAC TTCATGAATA 
     7561 ATGAAATCAC GGCTAGTAAA ATTGATGATG GTAATAATTC AAAACCACTG TCACCTGGTT 
     7621 GGACGGACCA AACTGCGTAT AACGCGTTTG GAATCACTAC AGGGATGTTT AATACCACTA 
     7681 CAATGGATGA TGTATATAAC TATCTATTCG ATGATGAAGA TACCCCACCA AACCCAAAAA 
     7741 AAGAGTAAAA TGAATCGTAG ATACTGAAAA ACCCCGCAAG TTCACTTCAA CTGTGCATCG 
     7801 TGCACCATCT CAATTTCTTT CATTTATACA TCGTTTTGCC TTCTTTTATG TAACTATACT 
     7861 CCTCTAAGTT TCAATCTTGG CCATGTAACC TCTGATCTAT AGAATTTTTT AAATGACTAG 
     7921 AATTAATGCC CATCTTTTTT TTGGACCTAA ATTCTTCATG AAAATATATT ACGAGGGCTT 
     7981 ATTCAGAAGC TTCGCTCATA TAACGAAAAA AAAGGGTTTG GATCGAACGT AATTGAGATT 
     8041 GATTAGTTAA TACTCAAAAT AAAACAGCTC CTACCACCAG TGTAAAGTAG AACGTTAATA 
     8101 GAGCAATGTC TTCAGACAGC GGCCGCGGAA GTGTCTCATA AACTTTACTC AAGAGTTAAG 
     8161 TACTGAAAAA TTCGACTTTT ATGATAGTTC AAGTGTCGAC GCTGCGGGTA TAGAAAGGGT 
     8221 TCTTTACTCT ATAGTACCTC CTCGCTCAGC ATCTGCTTCT TCCCAAAGAT GAACGCGGCG 
     8281 TTATGTCACT AACGACGTGC ACCAACTTGC GGAAAGTGGA ATCCCGTTCC AAAACTGGCA 
     8341 TCCACTAATT GATACATCTA CACACCGCAC GCCTTTTTTC TGAAGCCCAC TTTCGTGGAC 
     8401 TTTGCCATAT GCAAAATTCA TGAAGTGTGA TACCAAGTCA GCATACACCT CACTAGGGTA 
     8461 GTTTCTTTGG TTGTATTGAT CATTTGGTTC ATCGTGGTTC ATTAATTTTT TTTCTCCATT 
     8521 GCTTTCTGGC TTTGATCTTA CTATCATTTG GATTTTTGTC GAAGGTTGTA GAATTGTATG 
     8581 TGACAAGTGG CACCAAGCAT ATATAAAAAA AAAAAGCATT ATCTTCCTAC CAGAGTTGAT 
     8641 TGTTAAAAAC GTATTTATAG CAAACGCAAT TGTAATTAAT TCTTATTTTG TATCTTTTCT 
     8701 TCCCTTGTCT CAATCTTTTA TTTTTATTTT ATTTTTCTTT TCTTAGTTTC TTTCATAACA 
     8761 CCAAGCAACT AATACTATAA CATACAATAA TAGGTACCAA AATGCAGATT TTCGTCAAGA 
     8821 CTTTGACCGG TAAAACCATA ACATTGGAAG TTGAATCTTC CGATACCATC GACAACGTTA 
     8881 AGTCGAAAAT TCAAGACAAG GAAGGTATCC CTCCAGATCA ACAAAGATTG ATCTTTGCCG 
     8941 GTAAGCAGCT AGAAGACGGT AGAACGCTGT CTGATTACAA CATTCAGAAG GAGTCCACCT 
     9001 TACATCTTGT GCTAAGGCTA AGAGGTGGTA GACATGGTTC TGGTATCATG GTTAGACCAT 
     9061 TGAACTGTAT CGTTGCTGTT TCTCAAAACA TGGGTATTGG AAAGAATGGT GACTTGCCTT 
     9121 GGCCACCATT GAGAAATGAG TTTAAGTACT TCCAGAGAAT GACCACCACT TCTTCAGTTG 
     9181 AAGGTAAACA AAACTTGGTC ATCATGGGTA GAAAGACCTG GTTTTCTATT CCAGAAAAGA 
     9241 ACAGGTTGTT GAAGGACAGA ATCAACATCG TTTTGTCCAG GGAATTGAAA GAACCACCAA 
     9301 GAGGTGCTCA TTTCTTGGCA AAATCTTTGG ATGATGCCTT GAGATTGATC GAACAACCAG 
     9361 AATTGGCTTC CAAGGTTGAT ATGGTTTGGA TTGTCGGTGG TTCATCTGTT TATCAAGAAG 
     9421 CCATGAATCA ACCAGGTCAT TTGAGGTTGT TCGTTACCAG AATCATGCAA GAGTTTGAGT 
     9481 CTGATACTTT CTTCCCTGAA ATTGACTTGG GCAAGTATAA GTTGTTGCCA GAATATCCAG 
     9541 GTGTCTTGTC TGAAGTTCAA GAAGAAAAGG GTATCAAGTA CAAGTTCGAA GTCTACGAAA 
     9601 AGAAGGATGG TGGTGGTGGT GGTGGTAATA CAAACGTTCC AATATTCAGT TCTCCGGTCA 
     9661 GAGATTTACC AAGGTCTTTC GAACAAAAAC ATTTAGCGGT TGTAGATGCA TTTTTCCAAA 
     9721 CATACCATGT CAAACCTGAT TTTATCGCTA GGTCTCCTGG CAGAGTAAAT CTGATTGGTG 
     9781 AGCATATAGA TTATTGCGAT TTTTCAGTTT TGCCATTAGC CATTGATGTG GATATGCTTT 
     9841 GCGCAGTTAA AATTTTAGAC GAAAAAAATC CATCCATTAC CTTAACAAAT GCGGACCCTA 
     9901 AATTTGCTCA GCGAAAGTTT GATCTGCCTT TAGATGGTTC CTACATGGCC ATAGATCCGT 
     9961 CTGTGTCGGA ATGGTCGAAT TACTTTAAAT GCGGACTACA TGTGGCACAT TCATACTTGA 
    10021 AAAAAATTGC TCCGGAAAGA TTTAATAATA CACCCTTAGT AGGTGCGCAG ATCTTTTGCC 
    10081 AGAGCGATAT TCCTACTGGT GGTGGACTCT CATCTGCATT TACTTGCGCG GCAGCACTAG 
    10141 CCACAATTAG AGCCAATATG GGAAAAAATT TTGATATTTC CAAAAAAGAC TTGACCCGCA 
    10201 TCACAGCGGT TGCTGAGCAC TATGTTGGAG TCAATAATGG TGGTATGGAT CAAGCAACGT 
    10261 CTGTTTATGG GGAAGAAGAT CATGCTCTAT ACGTAGAGTT TAGGCCAAAA CTAAAGGCCA 
    10321 CACCTTTCAA GTTTCCTCAA TTGAAAAATC ATGAAATCAG TTTCGTCATC GCCAATACTC 
    10381 TTGTAAAGTC TAATAAATTC GAAACTGCTC CTACAAATTA CAATTTAAGA GTAATAGAGG 
    10441 TAACAGTTGC TGCCAACGCC TTGGCGACCA GATACAGCGT GGCCTTACCA TCTCACAAGG 
    10501 ACAATTCTAA CTCAGAAAGA GGGAATCTAA GAGATTTTAT GGATGCTTAC TACGCCAGAT 
    10561 ACGAAAACCA AGCCCAACCA TGGAATGGAG ATATCGGAAC TGGTATTGAA CGTTTACTCA 
    10621 AGATGCTACA ATTGGTAGAA GAATCTTTCT CGAGGAAAAA GAGCGGATTC ACTGTACATG 
    10681 AAGCCTCTAC GGCGCTAAAC TGTTCACGTG AGGAGTTCAC TAGAGATTAC CTGACAACTT 
    10741 TTCCCGTCCG CTTCCAAGTC TTGAAACTAT ATCAAAGAGC TAAACACGTT TACTCCGAAT 
    10801 CCTTAAGGGT GCTTAAGGCT TTAAAAATGA TGACAAGTGC CACTTTTCAC ACGGACGAAG 
    10861 ATTTCTTTAC AGATTTTGGC CGACTAATGA ATGAGTCCCA GGCCTCTTGT GATAAACTTT 
    10921 ATGAATGTTC GTGCATAGAA ACCAATCAAA TATGCTCGAT TGCCCTAGCA AATGGTTCTT 
    10981 TCGGCTCCCG TCTCACTGGC GCTGGTTGGG GCGGTTGCAC TATCCACCTT GTTCCTAGTG 
    11041 GCGCTAATGG GAACGTGGAA CAGGTACGAA AAGCACTAAT CGAAAAATTC TACAATGTAA 
    11101 GATATCCGGA TCTCACAGAT GAAGAACTAA AAGACGCAAT TATAGTTCCA AAGCCTGCCT 
    11161 TGGGTACTTG TTTGTACGAA CAATAAAGTG CTTTTAACTA AGAATTATTA GTCTTTTCTG 
    11221 CTTATTTTTT CATCATAGTT TAGAACACTT TATATTAACG AATAGTTTAT GAATCTATTT 
    11281 AGGTTTAAAA ATTGATACAG TTTTATAAGT TACTTTTTCA AAGACTCGTG CTGTCTATTG 
    11341 CATAATGCAC TGGAAGGGGA AAAAAAAGGT GCACACGCGT GGCTTTTTCT TGAATTTGCA 
    11401 GTTTGAAAAA TAACTACATG TAAGG 
//