LOCUS       pToxAMP1                6392 bp    DNA     circular     08-Nov-2021
FEATURES             Location/Qualifiers
     misc_feature    complement(405..477)
                     /label=ori
                     /note=origin of replication
     misc_feature    404..498
                     /label=ori
                     /note=/SECDrawAs=Region
     misc_feature    complement(2269..2291)
                     /label=pGEX_3_primer
                     /note=misc_feature
     promoter        complement(2082..2110)
                     /label=AmpR_promoter
                     /note=/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*
     CDS             complement(1335..1343)
                     /label=Linker(CDS)
                     /note=CDS
     misc_feature    complement(421..1009)
                     /label=rep
                     /note=misc_feature
     misc_feature    complement(2490..2633)
                     /label=lacZ_a
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    2604..2626
                     /label=M13_pUC_fwd_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        2619..2635
                     /label=M13_forward20_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        complement(15..33)
                     /label=M13_reverse_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    complement(32..54)
                     /label=M13_pUC_rev_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    2646..2898
                     /label=HO (-253,-1)
                     /note=misc_feature
     CDS             complement(1180..2110)
                     /label=bla
                     /note=CDS
     CDS             4669..5397
                     /label=yEGFP
                     /note=Detected CDS 242aa
     misc_feature    5404..5604
                     /label=URA3(705, 905)
                     /note=misc_feature
     misc_feature    4083..4668
                     /label=TEF1P
                     /note=misc_feature
     terminator      3777..4076
                     /label=PDC1 terminator
                     /note=terminator
     CDS             3529..3768
                     /label=relB
                     /note=CDS
     promoter        2905..3522
                     /label=RPL8B
                     /note=promoter
     rep_origin      5613..5881
                     /label=ARS712
                     /note=rep_origin
     misc_feature    5888..6387
                     /label=HO (-731, -264)
                     /note=misc_feature
     CDS             complement(1335..1343)
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     CDS             complement(1335..1343)
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
BASE COUNT     1851 a      1362 c      1323 g      1856 t 
ORIGIN
        1 GCATGCGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT 
       61 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG 
      121 CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG 
      181 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC 
      241 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC 
      301 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
      361 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
      421 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
      481 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
      541 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
      601 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
      661 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
      721 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
      781 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
      841 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
      901 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
      961 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     1021 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     1081 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     1141 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA 
     1201 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT 
     1261 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG 
     1321 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA 
     1381 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA 
     1441 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG 
     1501 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC 
     1561 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC 
     1621 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA 
     1681 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC 
     1741 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG 
     1801 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC 
     1861 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG 
     1921 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC 
     1981 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT 
     2041 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 
     2101 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 
     2161 AGTGCCACCT GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG 
     2221 TATCACGAGG CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT 
     2281 GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG 
     2341 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA 
     2401 GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG 
     2461 AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC 
     2521 GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT 
     2581 AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCATG 
     2641 CGTTTAAACC ACGAAAAGTT CACCATAACT TCGAATAAAG TCGCGGAAAA AAGTAAACAG 
     2701 CTATTGCTAC TCAAATGAGG TTTGCAGAAG CTTGTTGAAG CATGATGAAG CGTTCTAAAC 
     2761 GCACTATTCA TCATTAAATA TTTAAAGCTC ATAAAATTGT ATTCAATTCC TATTCTAAAT 
     2821 GGCTTTTATT TCTATTACAA CTATTAGCTC TAAATCCATA TCCTCATAAG CAGCAATCAA 
     2881 TTCTATCTAT ACTTTAAACC GCGGGCCTTG CGGAGGATAA CCACCAACGA ACTCAAATAC 
     2941 CTACCGCGTA CCTTCCTACG GTTATGTACT TTTGCACTGC TATTGCTTTG AGATGCTATG 
     3001 AGAACTGCCG TCAAAATTGG GTGGCAAAGA GGGATAGCTT CACTGCCAAT CACTACACTC 
     3061 TGTTCTGAAG GAGGGGAATT ACTGTTGTCT CATAAAAAAA CACCTTTGAA ACACTTTCAC 
     3121 TCATAGAGAA GCCAAGAACT CTGATGAGTT CTTTATTTGT TGCCGCTAAC TTTATCTTAT 
     3181 CTTCTCCATT GTAATAATTA CTATTCTTTT TACTTGCCTG TTTTCCATCC CAATGAAGAT 
     3241 CCGTACATCT TAGACACCCT TTTCTTTTTT TCTTTTTTTT TTTTTTTTTT TTTTTTGGCT 
     3301 TCCGAAGTGC CGACAACTAA AGAATTATCG CATTTTTTTT GAATACATCA TACTATACGA 
     3361 TAGAACGCAT TGAAACTTTT CCCATCTCAA AATCCAGGGA CAATAGTATG GGATCGATAT 
     3421 TATTATCAAG ACATAAGTAT TGTGATGCGC ACGTTGAAAT TCACTATTTT TTTTTTTTAT 
     3481 ATCTGTTTTT CAATACAGTG AACACGATAA CTGAAAGAAA CATCTAGAAT GGGTAGCATT 
     3541 AACCTGCGTA TTGACGATGA ACTTAAAGCG CGTTCTTACG CCGCGCTTGA AAAAATGGGT 
     3601 GTAACTCCTT CTGAAGCGCT TCGTCTCATG CTCGAGTATA TCGCTGACAA TGAACGCTTG 
     3661 CCGTTCAAAC AGACACTCCT GAGTGATGAA GATGCTGAAC TTGTGGAGAT AGTGAAAGAA 
     3721 CGGCTTCGTA ATCCTAAGCC AGTACGTGTG ACGCTGGATG AACTCTGACC TGCAGGGCGA 
     3781 TTTAATCTCT AATTATTAGT TAAAGTTTTA TAAGCATTTT TATGTAACGA AAAATAAATT 
     3841 GGTTCATATT ATTACTGCAC TGTCACTTAC CATGGAAAGA CCAGACAAGA AGTTGCCGAC 
     3901 AGTCTGTTGA ATTGGCCTGG TTAGGCTTAA GTCTGGGTCC GCTTCTTTAC AAATTTGGAG 
     3961 AATTTCTCTT AAACGATATG TATATTCTTT TCGTTGGAAA AGATGTCTTC CAAAAAAAAA 
     4021 ACCGATGAAT TAGTGGAACC AAGGAAAAAA AAAGAGGTAT CCTTGATTAA GGAACAGAGC 
     4081 TCCGTATAAA CAATGCATAC TTTGTACGTT CAAAATACAA TGCAGTAGAT ATATTTATGC 
     4141 ATATTACATA TAATACATAT CACATAGGAA GCAACAGGCG CGTTGGACTT TTAATTTTCG 
     4201 AGGACCGCGA ATCCTTACAT CACACCCAAT CCCCCACAAG TGATCCCCCA CACACCATAG 
     4261 CTTCAAAATG TTTCTACTCC TTTTTTACTC TTCCAGATTT TCTCGGACTC CGCGCATCGC 
     4321 CGTACCACTT CAAAACACCC AAGCACAGCA TACTAAATTT CCCCTCTTTC TTCCTCTAGG 
     4381 GTGTCGTTAA TTACCCGTAC TAAAGGTTTG GAAAAGAAAA AAGAGACCGC CTCGTTTCTT 
     4441 TTTCTTCGTC GAAAAAGGCA ATAAAAATTT TTATCACGTT TCTTTTTCTT GAAAATTTTT 
     4501 TTTTTTGATT TTTTTCTCTT TCGATGACCT CCCATTGATA TTTAAGTTAA TAAACGGTCT 
     4561 TCAATTTCTC AAGTTTCAGT TTCATTTTTC TTGTTCTATT ACAACTTTTT TTACTTCTTG 
     4621 CTCATTAGAA AGAAAGCATA GCAATCTAAT CTAAGTTTTA ATTACAAAAT GGGATCCTCT 
     4681 AAAGGTGAAG AATTATTCAC TGGTGTTGTC CCAATTTTGG TTGAATTAGA TGGTGATGTT 
     4741 AATGGTCACA AATTTTCTGT CTCCGGTGAA GGTGAAGGTG ATGCTACTTA CGGTAAATTG 
     4801 ACCTTAAAAT TTATTTGTAC TACTGGTAAA TTGCCAGTTC CATGGCCAAC CTTAGTCACT 
     4861 ACTTTCGGTT ATGGTGTTCA ATGTTTTGCG AGATACCCAG ATCATATGAA ACAACATGAC 
     4921 TTTTTCAAGT CTGCCATGCC AGAAGGTTAT GTTCAAGAAA GAACTATTTT TTTCAAAGAT 
     4981 GACGGTAACT ACAAGACCAG AGCTGAAGTC AAGTTTGAAG GTGATACCTT AGTTAATAGA 
     5041 ATCGAATTAA AAGGTATTGA TTTTAAAGAA GATGGTAACA TTTTAGGTCA CAAATTGGAA 
     5101 TACAACTATA ACTCTCACAA TGTTTACATC ATGGCTGACA AACAAAAGAA TGGTATCAAA 
     5161 GTTAACTTCA AAATTAGACA CAACATTGAA GATGGTTCTG TTCAATTAGC TGACCATTAT 
     5221 CAACAAAATA CTCCAATTGG TGATGGTCCA GTCTTGTTAC CAGACAACCA TTACTTATCC 
     5281 ACTCAATCTG CCTTATCCAA AGATCCAAAC GAAAAGAGAG ACCACATGGT CTTGTTAGAA 
     5341 TTTGTTACTG CTGCTGGTAT TACCCATGGT ATGGATGAAT TGTACAAAAG ATCTTAAACT 
     5401 AGTAGGACTA TTTGCAAAGG GAAGGGATGC TAAGGTAGAG GGTGAACGTT ACAGAAAAGC 
     5461 AGGCTGGGAA GCATATTTGA GAAGATGCGG CCAGCAAAAC TAAAAAACTG TATTATAAGT 
     5521 AAATGCATGT ATACTAAACT CACAAATTAG AGCTTCAATT TAATTATATC AGTTATTACC 
     5581 CGGGAATCTC GGTCGTAATG ATTTGCGGCC GCATTCCCAG AAGATTAGGG AGGTAATTAA 
     5641 TTAATAGGCA GATTTCTAGT TCTTATATAT TACTATAGAT AAGGAAATTA TACGCTTGTA 
     5701 TGCAGAGATA ATTTAAGTTT TGCTTTGTTC GCCACCATTC TAGTTATGAC CTTTATCTAT 
     5761 CATTCTATTT TTTATTGGAA AGGCTTTACA AAAAACAAAC AGTAAAAATT CTTTAAGCGA 
     5821 AGTGAAATCG ACAATGAATA TGAAATTGGT AATTATCTTA CATTAGGACT TTTCAATATC 
     5881 TGCCGGCAGG ATCCACGAAA ATGATGTGAA TGAATACATG AAAGATTCAT GAGATCTGAC 
     5941 AACATGGTAG ACGTGTGTGT CTCATGGAAA TTGATGCAGT TGAAGACATG TGCGTCACGA 
     6001 AAAAAGAAAT CAATCCTACA CAGGGCTTAA GGGCAAATGT ATTCATGTGT GTCACGAAAA 
     6061 GTGATGTAAC TAAATACACG ATTACCATGG AAATTAACGT ACCTTTTTTG TGCGTGTATT 
     6121 GAAATATTAT GACATATTAC AGAAAGGGTT CGCAAGTCCT GTTTCTATGC CTTTCTCTTA 
     6181 GTAATTCACG AAATAAACCT ATGGTTTACG AAATGATCCA CGAAAATCAT GTTATTATTT 
     6241 ACATCAACAT ATCGCGAAAA TTCATGTCAT GTCCACATTA ACATCATTGC AGAGCAACAA 
     6301 TTCATTTTCA TAGAGAAATT TGCTACTATC ACCCACTAGT ACTACCATTG GTACCTACTA 
     6361 CTTTGAATTG TACTACCGCT GGGCGTTTAA AC 
//