LOCUS       In-Vivo\Anneal          6127 bp    DNA     circular     11-Dec-2023
FEATURES             Location/Qualifiers
     misc_feature    complement(3660..3732)
                     /label=ori
                     /note=origin of replication
     misc_feature    3659..3753
                     /label=ori
                     /note=/SECDrawAs=Region
     misc_feature    complement(5524..5546)
                     /label=pGEX_3_primer
                     /note=misc_feature
     promoter        complement(5337..5365)
                     /label=AmpR_promoter
                     /note=/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*
     CDS             complement(4590..4598)
                     /label=Linker(CDS)
                     /note=CDS
     CDS             complement(1931..1939)
                     /label=Linker(CDS)(CDS)
                     /note=CDS
     misc_feature    complement(3676..4264)
                     /label=rep
                     /note=misc_feature
     misc_feature    complement(5745..5888)
                     /label=lacZ_a
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    5859..5881
                     /label=M13_pUC_fwd_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        5874..5890
                     /label=M13_forward20_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        complement(3270..3288)
                     /label=M13_reverse_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    complement(3287..3309)
                     /label=M13_pUC_rev_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    5901..26
                     /label=HO (-253,-1)
                     /note=misc_feature
     CDS             complement(4435..5365)
                     /label=bla
                     /note=CDS
     terminator      817..1116
                     /label=PDC1 terminator
                     /note=terminator
     CDS             569..808
                     /label=relB
                     /note=CDS
     rep_origin      2476..2744
                     /label=ARS712
                     /note=rep_origin
     misc_feature    2751..3250
                     /label=HO (-731, -264)
                     /note=misc_feature
     misc_feature    1123..1154
                     /label=synth6 terminator
                     /note=misc_feature
     terminator      2436..2467
                     /label=synth7 terminator
                     /note=terminator
     CDS             1740..2435
                     /label=AeBlue
                     /note=CDS
     promoter        1155..1739
                     /label=TDH3 promoter
                     /note=promoter
     CDS             complement(1931..1939)
                     /label=Linker(CDS)(CDS)
                     /note=CDS
     CDS             complement(4590..4598)
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     CDS             complement(1931..1939)
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     CDS             complement(4590..4598)
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     promoter        33..568
                     /label=CTR1 promoter
                     /note=promoter
     rep_origin      3660..3732
                     /label=AAG34515.1
                     /note=rep_origin
     CDS             complement(1931..1939)
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     CDS             complement(4590..4598)
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     CDS             complement(1931..1939)
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     CDS             complement(4590..4598)
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     CDS             complement(1931..1939)
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     CDS             complement(4590..4598)
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     misc_feature    4435..5295
                     /label=bla
                     /note=misc_feature
     CDS             complement(1931..1939)
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     CDS             complement(4590..4598)
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     Primer          complement(5873..5891)
                     /label=pUC19a1
                     /note=Primer
     Primer          complement(5873..5891)
                     /label=PGFPPUC19a
                     /note=Primer
     Primer          33..52
                     /label=PGFPCTR1s
                     /note=Primer
     Primer          complement(539..568)
                     /label=PGFPCTR1a
                     /note=Primer
     Primer          817..846
                     /label=PRP4PDC1ts
                     /note=Primer
     Primer          complement(1091..1116)
                     /label=PRP4PDC1ta
                     /note=Primer
BASE COUNT     1799 a      1290 c      1303 g      1735 t 
ORIGIN
        1 GCAATCAATT CTATCTATAC TTTAAACCGC GGACCGTATT TTGCTCGTTC CAGCCTTTCC 
       61 ACGTTTTTGT TATCTAAGCA ACTTGGCACA TTTCCCTACT ATACTACAAA CCGATACGTA 
      121 AATACTTCCC TAAATAGCAT ATGAATTATT CAGTAATTTT TAAGGATCGA AACTGCACCT 
      181 CAACTATTCG TTACTGTGGT TATGTTCTCA TGTATTGATG CAAATCATGG GATATTTGCT 
      241 CAAGACGACG GTAAAATGAG CAAAAATGGC ACGATCCTGA AAAGAGCACT TTTCAAGATT 
      301 CGGGCTACAA AATGCAACAT AAAAAATGTT GTATTGTCAT CTCGAGAGGG TCTTGTATGT 
      361 TTTATTCCTC TTATGATTAG TTCACATTAG TAAAACAGAT ACGCAGTGTG CTCTTAATAA 
      421 ACAACTACTC CATAGCTTTA TTTGCATAAC AAAACTTTTA AGCACAAACT TAAACAGGTG 
      481 GAGTAATAGT TCGGCGGCGA CTCAAATTAC ATTTGTTGGA AGAATCGAAT AGAAAATAAA 
      541 AAAAAGTGTA TTATATTTGA CATTCAAAAT GGGTAGCATT AACCTGCGTA TTGACGATGA 
      601 ACTTAAAGCG CGTTCTTACG CCGCGCTTGA AAAAATGGGT GTAACTCCTT CTGAAGCGCT 
      661 TCGTCTCATG CTCGAGTATA TCGCTGACAA TGAACGCTTG CCGTTCAAAC AGACACTCCT 
      721 GAGTGATGAA GATGCTGAAC TTGTGGAGAT AGTGAAAGAA CGGCTTCGTA ATCCTAAGCC 
      781 AGTACGTGTG ACGCTGGATG AACTCTGACC TGCAGGGCGA TTTAATCTCT AATTATTAGT 
      841 TAAAGTTTTA TAAGCATTTT TATGTAACGA AAAATAAATT GGTTCATATT ATTACTGCAC 
      901 TGTCACTTAC CATGGAAAGA CCAGACAAGA AGTTGCCGAC AGTCTGTTGA ATTGGCCTGG 
      961 TTAGGCTTAA GTCTGGGTCC GCTTCTTTAC AAATTTGGAG AATTTCTCTT AAACGATATG 
     1021 TATATTCTTT TCGTTGGAAA AGATGTCTTC CAAAAAAAAA ACCGATGAAT TAGTGGAACC 
     1081 AAGGAAAAAA AAAGAGGTAT CCTTGATTAA GGAACAGAGC TCTATATATT TAATAAAGAG 
     1141 TATCATCTTT CAAAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA ATAGGGGGCG 
     1201 GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC CTGGCATCCA 
     1261 CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA ATCCCAGCAC 
     1321 CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA GCGCAACTAC 
     1381 AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT GATGCAACCT 
     1441 GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC TCATTTTCTT 
     1501 ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA AAGGTTGAAA 
     1561 CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG GTAGGTATTG 
     1621 ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT AGTTAGTCTT 
     1681 TTTTTTAGTT TTAAAACACC AAGAACTTAG TTTCGAATAA ACACACATAA ACAAACAAAA 
     1741 TGGCTTCTTT GGTTAAGAAA GATATGTGTA TCAAGATGAC TATGGAAGGT ACTGTTAACG 
     1801 GTCATCACTT TAAGTGTGTT GGTGAAGGTG AAGGTAAACC ATTTGAAGGT ACTCAAGTTG 
     1861 AAAAGATTAG AATTACTGAA GGTGGTCCAT TGCCATTTGC TTATGATATT TTGGCTCCAT 
     1921 GTTGTATGTA TGGTTCTAAG ACTTTCATTA AGCACGTTTC TGGTATCCCA GACTACTTCA 
     1981 AAGAATCTTT TCCAGAAGGT TTTACCTGGG AAAGAACTCA AATCTTTGAA GATGGTGGTT 
     2041 ACTTGACTAT TCACCAAGAT ACTTCATTGC AAGGTAACAA CTTTATCTTC AAAGTTAACG 
     2101 TTATCGGTGC TAATTTTCCA GCTAATGGTC CAGTTATGCA AAAGAAAACT GCTGGTTGGG 
     2161 AACCATGTGT TGAAATGTTG TATCCAAGAG ATGGTGTTTT GTGCGGTCAA TCTTTGATGG 
     2221 CTTTGAAATG TACTGATGGT AATCACTTGA CCTCTCATTT GAGAACTACT TACAGATCCA 
     2281 GAAAACCATC TAACGCTGTT AACATGCCAG AATTTCATTT CGGTGACCAC AGAATTGAGA 
     2341 TTTTGAAAGC TGAACAAGGT AAGTTCTACG AACAATACGA ATCTGCTGTT GCTAGATATT 
     2401 GTGAAGCTGC TCCATCAAAA TTGGGTCATC ATTAATATAT AACTGTCTAG AAATAAATTT 
     2461 TTTCAAAGCG GCCGCATTCC CAGAAGATTA GGGAGGTAAT TAATTAATAG GCAGATTTCT 
     2521 AGTTCTTATA TATTACTATA GATAAGGAAA TTATACGCTT GTATGCAGAG ATAATTTAAG 
     2581 TTTTGCTTTG TTCGCCACCA TTCTAGTTAT GACCTTTATC TATCATTCTA TTTTTTATTG 
     2641 GAAAGGCTTT ACAAAAAACA AACAGTAAAA ATTCTTTAAG CGAAGTGAAA TCGACAATGA 
     2701 ATATGAAATT GGTAATTATC TTACATTAGG ACTTTTCAAT ATCTGCCGGC AGGATCCACG 
     2761 AAAATGATGT GAATGAATAC ATGAAAGATT CATGAGATCT GACAACATGG TAGACGTGTG 
     2821 TGTCTCATGG AAATTGATGC AGTTGAAGAC ATGTGCGTCA CGAAAAAAGA AATCAATCCT 
     2881 ACACAGGGCT TAAGGGCAAA TGTATTCATG TGTGTCACGA AAAGTGATGT AACTAAATAC 
     2941 ACGATTACCA TGGAAATTAA CGTACCTTTT TTGTGCGTGT ATTGAAATAT TATGACATAT 
     3001 TACAGAAAGG GTTCGCAAGT CCTGTTTCTA TGCCTTTCTC TTAGTAATTC ACGAAATAAA 
     3061 CCTATGGTTT ACGAAATGAT CCACGAAAAT CATGTTATTA TTTACATCAA CATATCGCGA 
     3121 AAATTCATGT CATGTCCACA TTAACATCAT TGCAGAGCAA CAATTCATTT TCATAGAGAA 
     3181 ATTTGCTACT ATCACCCACT AGTACTACCA TTGGTACCTA CTACTTTGAA TTGTACTACC 
     3241 GCTGGGCGTT TAAACGCATG CGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG 
     3301 TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 
     3361 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC 
     3421 GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 
     3481 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 
     3541 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     3601 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     3661 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     3721 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     3781 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     3841 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     3901 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     3961 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4021 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4081 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4141 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     4201 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     4261 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     4321 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     4381 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 
     4441 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 
     4501 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 
     4561 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 
     4621 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 
     4681 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 
     4741 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 
     4801 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 
     4861 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 
     4921 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 
     4981 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 
     5041 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 
     5101 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 
     5161 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 
     5221 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 
     5281 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 
     5341 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 
     5401 CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC 
     5461 CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA 
     5521 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG 
     5581 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA 
     5641 CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA 
     5701 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG 
     5761 TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG 
     5821 TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC 
     5881 GACGGCCAGT GCATGCGTTT AAACCACGAA AAGTTCACCA TAACTTCGAA TAAAGTCGCG 
     5941 GAAAAAAGTA AACAGCTATT GCTACTCAAA TGAGGTTTGC AGAAGCTTGT TGAAGCATGA 
     6001 TGAAGCGTTC TAAACGCACT ATTCATCATT AAATATTTAA AGCTCATAAA ATTGTATTCA 
     6061 ATTCCTATTC TAAATGGCTT TTATTTCTAT TACAACTATT AGCTCTAAAT CCATATCCTC 
     6121 ATAAGCA 
//