LOCUS       In-Vivo\Anneal          5498 bp    DNA     circular     06-Nov-2021
FEATURES             Location/Qualifiers
     misc_feature    complement(1194..1266)
                     /label=ori
                     /note=origin of replication
     promoter        complement(4477..5476)
                     /label=DDI2 promoter
                     /note=promoter
     misc_feature    249..592
                     /label=TEFp
                     /note=TEF1 promoter of Ashbya gossypii
     misc_feature    1193..1287
                     /label=ori
                     /note=/SECDrawAs=Region
     misc_feature    complement(3058..3080)
                     /label=pGEX_3_primer
                     /note=misc_feature
     promoter        complement(2871..2899)
                     /label=AmpR_promoter
                     /note=/translation=MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*
     CDS             complement(2124..2132)
                     /label=Linker(CDS)
                     /note=CDS
     misc_feature    complement(1210..1798)
                     /label=rep
                     /note=misc_feature
     misc_feature    complement(3279..3422)
                     /label=lacZ_a
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    3393..3415
                     /label=M13_pUC_fwd_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        3408..3424
                     /label=M13_forward20_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     promoter        complement(804..822)
                     /label=M13_reverse_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    complement(821..843)
                     /label=M13_pUC_rev_primer
                     /note=/translation=MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLS IPSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEE ARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLA TFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*
     misc_feature    3435..3687
                     /label=HO (-253,-1)
                     /note=misc_feature
     misc_feature    4..177
                     /label=HO (1655, 1828)
                     /note=misc_feature
     CDS             complement(3763..4467)
                     /label=I-SceI
                     /note=CDS
     CDS             complement(1969..2899)
                     /label=bla
                     /note=CDS
     terminator      complement(3688..3756)
                     /label=synth3 terminator
                     /note=terminator
     misc_feature    4..90
                     /label=HO(1655, 1741)
                     /note=misc_feature
     misc_feature    91..177
                     /label=HO (1742, 1828)
                     /note=misc_feature
     misc_feature    178..207
                     /label=I-SceI
                     /note=misc_feature
     CDS             complement(2124..2132)
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
     misc_feature    186..203
                     /label=I-SceI site
                     /note=misc_feature
     promoter        210..592
                     /label=AgTEF1 promoter
                     /note=promoter
     CDS             complement(2124..2132)
                     /label=Linker(CDS)(CDS)(CDS)
                     /note=CDS
BASE COUNT     1484 a      1199 c      1278 g      1537 t 
ORIGIN
        1 TCTCAGTTAA AGGACCAGAG TGTATAAAAT GTGGCGGAAT CTTACAGTTT GATGCTGTCC 
       61 GCGGGCCTCA TAAGAGTTGT GGTAACAACG CAGGTGCGCG CATCTGCTAA AATGTGTATA 
      121 TTAGTTTAAA AAGTTGTATG TAATAAAAGT AAAATTTAAT ATTTTGGATG AAAAAAAAGT 
      181 TACGCTAGGG ATAACAGGGT AATATAGCTG TTTAGCTTGC CTCGTCCCCG CCGGGTCACC 
      241 CGGCCAGCGA CATGGAGGCC CAGAATACCC TCCTTGACAG TCTTGACGTG CGCAGCTCAG 
      301 GGGCATGATG TGACTGTCGC CCGTACATTT AGCCCATACA TCCCCATGTA TAATCATTTG 
      361 CATCCATACA TTTTGATGGC CGCACGGCGC GAAGCAAAAA TTACGGCTCC TCGCTGCAGA 
      421 CCTGCGAGCA GGGAAACGCT CCCCTCACAG ACGCGTTGAA TTGTCCCCAC GCCGCGCCCC 
      481 TGTAGAGAAA TATAAAAGGT TAGGATTTGC CACTGAGGTT CTTCTTTCAT ATACTTCCTT 
      541 TTAAAATCTT GCTAGGATAC AGTTCTCACA TCACATCCGA ACATAAACAA CCATGGGTAA 
      601 AAAGCCTGAA CTCACCGCGA CGTCTGTCGA GAAGTTTCTG ATCGAAAAGT TCGACAGCGT 
      661 CTCCGACCTG ATGCAGCTCT CGGAGGGCGA AGAATCTCGT GCTTTCAGCT TCGATGTAGG 
      721 AGGGCGTGGA TATGTCCTGC GGGTAAATAG CTGCGCCGAT GGTTTCTACA AAGATCGTTA 
      781 TGTTTAAACG CATGCGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC 
      841 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA 
      901 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA 
      961 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 
     1021 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG 
     1081 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     1141 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     1201 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     1261 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     1321 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     1381 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     1441 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     1501 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     1561 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     1621 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     1681 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     1741 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     1801 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     1861 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     1921 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 
     1981 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 
     2041 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 
     2101 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 
     2161 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 
     2221 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 
     2281 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 
     2341 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 
     2401 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 
     2461 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 
     2521 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 
     2581 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 
     2641 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 
     2701 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 
     2761 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 
     2821 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 
     2881 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT 
     2941 TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA 
     3001 AAATAGGCGT ATCACGAGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT 
     3061 CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG 
     3121 ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC 
     3181 GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG 
     3241 CGTAAGGAGA AAATACCGCA TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA 
     3301 AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC 
     3361 AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC 
     3421 CAGTGCATGC GTTTAAACCA CGAAAAGTTC ACCATAACTT CGAATAAAGT CGCGGAAAAA 
     3481 AGTAAACAGC TATTGCTACT CAAATGAGGT TTGCAGAAGC TTGTTGAAGC ATGATGAAGC 
     3541 GTTCTAAACG CACTATTCAT CATTAAATAT TTAAAGCTCA TAAAATTGTA TTCAATTCCT 
     3601 ATTCTAAATG GCTTTTATTT CTATTACAAC TATTAGCTCT AAATCCATAT CCTCATAAGC 
     3661 AGCAATCAAT TCTATCTATA CTTTAAATTT GAAAGATGAT ACTCTTTATT TCTAGACAGT 
     3721 TATATATATA TATATATATA TATATATATA TATATACTCG AGTTATTACT TAAGGAAAGT 
     3781 TTCGGAGGAG ATAGTGTTCG GCAGTTTGTA CATCATCTGC GGGATCAGGT ACGGTTTGAT 
     3841 CAGGTTGTAG AAGATCAGGT AAGACATAGA ATCGATGTAG ATGATCGGTT TGTTTTTGTT 
     3901 GATTTTTACG TAACAGTTCA GTTGGAATTT GTTACGCAGA CCCTTAACCA GGTATTCTAC 
     3961 TTCTTCGAAA GTGAAAGACT GGGTGTTCAG TACGATCGAT TTGTTGGTAG AGTTTTTGTT 
     4021 GTAATCCCAT TTACCACCAT CATCCATGAA CCAGTATGCC AGAGACATCG GGGTCAGGTA 
     4081 GTTTTCAACC AGGTTGTTCG GGATGGTTTT TTTGTTGTTA ACGATGAACA GGTTAGCCAG 
     4141 TTTGTTGAAG GCTTGGTGTT TGAAAGTCTG GGCGCCCCAG GTGATTACCA GGTTACCCAG 
     4201 GTGGTTAACA CGTTCTTTTT TGTGCGGCGG GGACAGTACC CACTGATCGT ACAGCAGACA 
     4261 TACGTGGTCC ATGTATGCTT TGTTTTTCCA CTCGAACTGC ATACAGTAGG TTTTACCTTC 
     4321 ATCACGAGAA CGGATGTAAG CATCACCCAG GATCAGACCG ATACCTGCTT CGAACTGTTC 
     4381 GATGTTCAGT TCGATCAGCT GGGATTTGTA TTCTTTCAGC AGTTTAGAGT TCGGACCCAG 
     4441 GTTCATTACC TGGTTTTTTT GATGCATTTT ACTAGTGATT GATTCTTTTG AAGAGAAGCA 
     4501 AGGCAAAAAA CAGTACGAAG CAACTTCATG TTAACAGAAG AAGAAGGAAA CTAACCCATT 
     4561 TATATATAAT TTTATGAGGG GACCGATCCT AAAAAGTTAT TTGTAAAGCT CGTACAAGAT 
     4621 TGATTGATGA CTTCCCGACA AACTATTGTG CGGTGGAGTA TTTTGTATTA TAGAAATGAT 
     4681 AGTGTCCATG CACTTTAATA AGATCTTTTT TGCGGACATT TGTTTCGGGG CCGTCCAAAG 
     4741 TTGTTTGATG GTATAACTCC GCATATTTGA CATCTTTCTC TTTTAAACCA GTATCAGTTA 
     4801 GTCTTTTGCA ATTTTCTTGA GGGATTCAGT CTAGAAAGTT GATTTTCATT GCTGTTAAGA 
     4861 TAAAAATTCC TTCTAACCCA GCTTGTACTC CGTATACTTT CAATGCTCTC AAAAAGATAT 
     4921 GGATACTAGT TAGTTTAGAC CCTGTAGTTT CACCAGCTGC TAGTATTCAA CGTTCATGCG 
     4981 ACTTCTATAA AGGAGTGCTT GGCTTTGATG TTACAACATT CGGGAACTAT CGCAGGGCTC 
     5041 TGTCTTTTTT TGGAGTCAGA AAAATCAATT TGCATGAGGT GGGAAAGGAA TTTGAGCCCA 
     5101 AGGCACAGAA GCCAACTCTC GGATCTGTCG ATCTGTGTTT TATCTTAGAG GATCCTTTGA 
     5161 ATAACAGTTA CCCATCTTGA AAGCTTGGAT TTTCCAATTG AAGAGGGCAC TGTAATCCGT 
     5221 ACAGGCGCAG TCAACCCTAT TATCAGTATA CATCCGTGAT TAAGATGAAA GTGTGATGGA 
     5281 AATATCAAAT AGAATAAAAG AAACGGAATA GAGCTATTTT TTTTTTATAT TATAGACATA 
     5341 GTCTAAGCGA GTTTAACCTT TGAAATATAA AAAAAAAAAA CAAAAGGAAA AAAGTAGTAT 
     5401 GTGGGCTGCA CTAAACTAAA ACCGTGAGGT ATTTAGATAT ATATTCTACA TTGTTTTACG 
     5461 TTATAGCATG TATAAAGAGC TCCAGCTTTT GTTCCCTT 
//