LOCUS       Exported                3217 bp ds-DNA     circular SYN 13-MAR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 14, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          57..76
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
     source          77..2102
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
     source          786..1799
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          795..1796
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     7..27
                     /label=pICOZ-colonyPCR-F
     primer_bind     36..76
                     /label=M13F-Tn
     primer_bind     36..56
                     /label=minicircle-F
     primer_bind     37..54
                     /locus_tag="M13F"
                     /label=M13F
                     /note="M13F;color: #a020f0; direction: RIGHT"
     enhancer        77..456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    77
     primer_bind     244..264
                     /label=CMV-qPCR-F110
     primer_bind     267..289
                     /label=CMV-qPCR-probeFAM
     primer_bind     328..347
                     /label=CMV-qPCR-F7
     primer_bind     333..352
                     /label=CMV-qPCR-F2
     primer_bind     complement(334..353)
                     /label=CMV-qPCR-R110
     promoter        457..660
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(457..477)
                     /label=CMV-qPCR-R7
     primer_bind     complement(458..477)
                     /label=CMV-qPCR-R2
     primer_bind     470..489
                     /label=CMV-qPCR-F5
     primer_bind     492..511
                     /label=CMV-qPCR-F1
     primer_bind     complement(585..604)
                     /label=CMV-qPCR-R1
     primer_bind     611..631
                     /label=CMV Forward
     promoter        705..723
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             795..1796
                     /codon_start=1
                     /label=MAG
                     /translation="MGRSAHSTQQQRLDIKRLSAAGYSQRKIAEILGRSKTFVYNALHS
                     TGTKIPTGRPRKTSARDDARMTRLCKADPFKSARAIRDELQLSVSDRTVQRRLFENNLV
                     GRNPRKVPLLRRCHVQARLQFAREHYDWAGENLNKWRNVLWSDESKVNLVGSDGKRFVR
                     RPKNTAYRPQYTLKTVKHGGGNIMVWACFSWYGVGPIFWIKDIMDQHRYLNIIQTVMLP
                     HAEWEMPLKWQFMHDNDPKHTAKAVKKWFVDQKIDVMNWPAQSPDLNPIENLWKIVKAK
                     LPPAGSRTKEKLWKHIENAWYSIPPSTCKSLVESMPKRMRAVIRNNGHATKY"
     CDS             1335..1337
                     /codon_start=1
                     /label=H181A/R gct/A gtt/V
                     /translation="H"
     CDS             1512..1514
                     /codon_start=1
                     /label=P240A/R gcg/A cgg/R
                     /translation="P"
     CDS             1515..1517
                     /codon_start=1
                     /label=K241R AGG/R
                     /translation="K"
     primer_bind     complement(1804..1824)
                     /label=IVT-Tn-R
     promoter        complement(1834..1852)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    1878..2102
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2002..2021)
                     /label=BGHLrev
     primer_bind     complement(2103..2125)
                     /label=minicircle-R
     primer_bind     complement(2104..2121)
                     /locus_tag="M13R"
                     /label=M13R
                     /note="M13R;color: #a020f0; direction: LEFT"
     primer_bind     2141..2160
                     /label=pICOZ-BB-qPCR-F127
     rep_origin      complement(2164..2385)
                     /direction=LEFT
                     /label=pMB1
     rep_origin      complement(2167..2755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2227..2246)
                     /label=pICOZ-BB-qPCR-probeFAM
     primer_bind     complement(2248..2267)
                     /label=pICOZ-BB-qPCR-R127
     primer_bind     complement(2390..2409)
                     /label=Primer 2
     CDS             complement(2780..3154)
                     /codon_start=1
                     /label=zeocin resistance
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     2892..2910
                     /label=pICOZ-BB-qPCR-F3
     primer_bind     2908..2926
                     /label=pICOZ-BB-qPCR-F10
     primer_bind     complement(2993..3010)
                     /label=pICOZ-BB-qPCR-R10
     primer_bind     3004..3023
                     /label=pICOZ-BB-qPCR-F1
     primer_bind     complement(3006..3025)
                     /label=pICOZ-BB-qPCR-R3
     primer_bind     3040..3059
                     /label=pICOZ-BB-qPCR-F2
     primer_bind     complement(3130..3149)
                     /label=pICOZ-BB-qPCR-R1
     RBS             3155..3188
                     /label=5' UTR of amp, incl. RBS
     promoter        complement(3189..3217)
                     /label=ampicillin resistance promoter
ORIGIN
        1 acgcgtcgcg aggccatatg ggttaaccca tggccttgta aaacgacggc cagtcgacgg
       61 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      121 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      181 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      241 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      301 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      361 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      421 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      481 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      541 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      601 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      661 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
      721 agggagaccc aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg
      781 aattcgccgc caccatgggt cgttcagcgc atagcaccca acaacagcgc ctggacatta
      841 aacgcttaag cgccgccggt tatagccaac gcaaaattgc ggaaatcctg ggccgcagca
      901 agacctttgt gtataacgcc ctgcatagca ccggcaccaa aattcctact ggccgccctc
      961 gtaaaactag cgcgcgtgat gatgcacgta tgacccgtct gtgtaaagcc gacccgttta
     1021 aaagcgcgcg cgccattcgt gatgaactgc agctgagcgt tagcgatcgc accgttcaac
     1081 gtcgcctgtt cgagaataat ctggtgggcc gcaatcctcg taaagtgcct ctgctgcgtc
     1141 gttgtcatgt gcaggcacgc ttacaatttg cgcgcgagca ctatgattgg gcgggcgaga
     1201 acttaaacaa atggcgcaac gtgctgtgga gcgatgagag caaagtgaac ctggtgggca
     1261 gcgatggcaa acgttttgtg cgccgcccta agaataccgc gtatcgccct cagtataccc
     1321 tgaaaaccgt gaaagctggc ggcggcaaca ttatggtgtg ggcgtgcttc tcatggtatg
     1381 gcgtgggccc tatcttctgg atcaaagaca tcatggatca gcatcgctac ctgaacatta
     1441 ttcagaccgt gatgctgcct cacgccgaat gggaaatgcc tctgaagtgg cagttcatgc
     1501 atgataacga cccgaagcat accgccaaag cggtgaagaa atggttcgtg gaccagaaga
     1561 ttgatgtgat gaactggcct gcgcagagcc ctgatctgaa cccgatcgaa aacctgtgga
     1621 agattgtgaa ggccaagctg cctcctgcag gttcacgcac caaagagaaa ctgtggaagc
     1681 atattgaaaa cgcgtggtac agcatacctc cgagcacctg taaaagcctg gtggaaagca
     1741 tgccgaaacg tatgcgcgcg gtgattcgca ataacggcca tgcgaccaaa tattgatgag
     1801 cggccgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagc
     1861 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     1921 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     1981 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     2041 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     2101 ggcggtcata gctgtttcct gtgtgggata tcctcgagac agtgggcccg tttaaacaca
     2161 tgtgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2221 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2281 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2341 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2401 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2461 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2521 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2581 gtggcctaac tacggctaca ctagaagaac agtatttggt atccgcgctc tgctgaagcc
     2641 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2701 cggtggtttt tttgtttgca agcagcagat tacgcgcagg aaaaaaggat ctcaagaaga
     2761 tcctttgatc ttttctacgt cagtcctgct cctcggccac gaagtgcacg cagttgccgg
     2821 ccgggtcgcg cagggcgaac tcccgccccc acggctgctc gccgatctcg gtcatggccg
     2881 gcccggaggc gtcccggaag ttcgtggaca cgacctccga ccactcggcg tacagctcgt
     2941 ccaggccgcg cacccacacc caggccaggg tgttgtccgg caccacctgg tcctggaccg
     3001 cgctgatgaa cagggtcacg tcgtcccgga ccacaccggc gaagtcgtcc tccacgaagt
     3061 cccgggagaa cccgagccgg tcggtccaga actcgaccgc tccggcgacg tcgcgcgcgg
     3121 tgagcaccgg aacggcactg gtcaacttgg ccatactctt ccttttcaat attattgaag
     3181 catttatcag ggttattgtc tcatgagcgg atacata
//
