LOCUS       Exported               13120 bp ds-DNA     circular SYN 12-JAN-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13120)
  AUTHORS   gaohui
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Mar 14, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13120
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      12..39
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(51..638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      660..689
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     724..743
                     /label=Ftw
                     /note="Ftw"
     primer_bind     751..768
                     /label=M13F
                     /note="M13F"
     primer_bind     752..768
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    820..1442
                     /label=5'TR
     promoter        1464..1963
                     /label=PGK promoter
                     /note="phosphoglycerate kinase promoter"
     CDS             1983..2582
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             2589..2645
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2646..3365
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    3393..3617
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        3657..4835
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3888..4826
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     misc_feature    join(4856..4858,4883..4942)
                     /locus_tag="CD8 N terminus"
                     /label=P4 N terminus
     CDS             4859..4882
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4943..5716
                     /locus_tag="P4"
                     /label=P4
     misc_feature    5717..5851
                     /locus_tag="CD8 alpha hinge"
                     /label=CD8 alpha hinge
     misc_feature    5852..6055
                     /locus_tag="CD28"
                     /label=CD28
     misc_feature    join(6056..6094,6096..6391)
                     /locus_tag="CD3 zeta"
                     /label=CD3 zeta
     misc_feature    6398..6598
                     /label=SV40 polyA
     enhancer        6627..8037
                     /label=HS2 1411
     misc_feature    6921..7389
                     /label=NCBI HS2
     enhancer        8038..9239
                     /label=HS3 1202bp
     misc_feature    8302..9211
                     /label=NCBI HS3
     promoter        9245..9504
                     /label=-260 HBB p
     misc_feature    9505..11112
                     /label=HBB gene
     misc_feature    9505..9646
                     /label=EXON 1
     misc_feature    9777..9999
                     /label=EXON2
     misc_feature    10850..11112
                     /label=EXON 3
     polyA_site      10979..11373
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     misc_feature    11413..11787
                     /label=3'TR
     primer_bind     complement(11823..11840)
                     /label=M13R
                     /note="M13R"
     primer_bind     complement(11824..11840)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11866..11882)
                     /label=Rtw
                     /note="Rtw"
     terminator      11921..11952
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        11953..12055
                     /label=cat promoter
                     /note="cat promoter"
     CDS             12056..12916
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             12917..13108
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 tgtaatcacc tggctcacct tcgggtgggc ctttctgcgt tgctggcgtt tttccatagg
       61 ctccgccccc ctgacgagca tcacaaaaat cgatgctcaa gtcagaggtg gcgaaacccg
      121 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      181 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      241 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      301 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      361 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      421 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      481 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac ctcggaaaaa
      541 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      601 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg attttctacc
      661 gaagaaaggc ccacccgtga aggtgagcca gtgagttgat tgcagtccag ttacgctgga
      721 gtcaataggc gtatcacgag gccctttcgt tgtaaaacga cggccagtcg aaccacgcaa
      781 tgcgtctcga tccgcagtgt cttgcgtctc tcaattgtat acagtgtcgg acaaatcaat
      841 aggaccactt atcaatttat tattcactta tcatatttca tgacttaaag tgaatcaatc
      901 tttaccaaac tttcatgaaa gcactattag aatgtttact ttaagaatgt ttttaaagtt
      961 tggtgaaaag ttaagaaaca aaaaagttat gataagaaat gtaaaaataa cccaaaaatc
     1021 atgcgccaaa ataataggac cagttcagaa aattttaaaa taattatcat ttattattgt
     1081 taaaacacag tttttgatgt tattgagttg tcggataact taacttaaat tagtgaacta
     1141 atgcgtattc attttttttg tagaaacgct tagcgcagtg gcaattcgct gtgtctttaa
     1201 ttaacatgct ctaatttact aatgcaagaa tattacggca ctcgcttaag ggttttgagc
     1261 tccctttgca ctgagagacg aaatagtggt gagtaccttc gtctatgggt ataaaagcgc
     1321 gtcacagcgc tggatcgctt acactgtgtt ctaagccgtt gcctagaaca cttcacacga
     1381 tccattagct cgtgtatttt tgggtgcact acttcaagcg cttgattgaa ggaattccaa
     1441 cgaccggtgc tagcaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
     1501 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
     1561 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
     1621 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
     1681 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
     1741 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
     1801 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
     1861 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
     1921 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gccaacgcca
     1981 ccatggggac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     2041 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     2101 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     2161 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     2221 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     2281 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     2341 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     2401 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     2461 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     2521 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     2581 ccatcgatgc cactaacttc tccctgttga aacaagcagg ggatgtcgaa gagaatcccg
     2641 ggccaatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     2701 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     2761 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     2821 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     2881 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     2941 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3001 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3061 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     3121 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3181 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3241 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3301 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     3361 agtaattaat taagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     3421 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     3481 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     3541 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     3601 tgcggtgggc tctatggctt ctgagggcgg ccgccatatg ggtaccatta acgtgaggct
     3661 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     3721 gggtcggcaa ttgacccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     3781 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     3841 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     3901 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     3961 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     4021 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
     4081 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     4141 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     4201 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     4261 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     4321 ggccaccgag aatcggacgg gggtagtctc aagctcgccg gcctgctctg gtgcctggcc
     4381 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     4441 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     4501 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     4561 cagccgtcgc ttcatgtgac tccactgagt accgggcgcc gtccaggcac ctcgattagt
     4621 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     4681 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     4741 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     4801 ttcaaagttt ttttcttcca tttcaggtgt cgtgagggat ccaccggtcg ccaccatgga
     4861 ttacaaagac gatgacgata aggccttacc agtgaccgcc ttgctcctgc cgctggcctt
     4921 gctgctccac gccgccaggc cgcaagtgca actccaacaa tctggccccg gtctggtcac
     4981 tccttcccag actcttagtc ttacctgtgc catatcaggt gattcagtga gtagtaactc
     5041 cgcgacatgg aattggatca ggcagtcccc cagtcgaggg ttggaatggc tcggtagaac
     5101 atactaccgc agtaaatggt ataatgatta tgctgtaagt gtgaaaagca ggatgtctat
     5161 aaatccagat acttccaaaa accagttctc tcttcagctt aatagtgtaa ctcccgaaga
     5221 cacagctgtt tactattgtg cgcggggtat gatgacttac tactatggta tggacgtttg
     5281 gggacaaggt acaaccgtca ccgtgtccag tgggatcctg ggctcaggcg gtggaggttc
     5341 cggtgggggc gggtccggag gggggggtag ccaacctgtt ctcacgcaat catcctccct
     5401 cagtgcaagc cccggtgcct ccgcttcttt gacgtgtaca ctgaggtcag gaatcaatgt
     5461 agggccttac aggatctact ggtatcagca aaaaccaggt tctcctcccc aataccttct
     5521 caattacaag agcgactcag ataagcagca gggatctgga gttccatcaa ggtttagtgg
     5581 atctaaagac gcaagcgcca atgcgggtgt gctgctcatc tcaggtctca ggtccgagga
     5641 cgaggccgat tattactgta tgatttggca tagttcagcc gctgttttcg gtggaggcac
     5701 gcaactgaca gtgctgacca cgacgccagc gccgcgacca ccaacaccgg cgcccaccat
     5761 cgcgtcgcag cccctgtccc tgcgcccaga ggcgtgccgg ccagcggcgg ggggcgcagt
     5821 gcacacgagg gggctggact tcgcctgtga tttttgggtg ctggtggtgg ttgggggagt
     5881 cctggcttgc tatagcttgc tagtaacagt ggcctttatt attttctggg tgaggagtaa
     5941 gaggagcagg ctcctgcaca gtgactacat gaacatgact ccccgccgcc ccgggcccac
     6001 ccgcaagcat taccagccct atgccccacc acgcgacttc gcagcctatc gctccagagt
     6061 gaagttcagc aggagcgcag acgcccccgc gtaccagcag ggccagaacc agctctataa
     6121 cgagctcaat ctaggacgaa gagaggagta cgatgttttg gacaagagac gtggccggga
     6181 ccctgagatg gggggaaagc cgagaaggaa gaaccctcag gaaggcctgt acaatgaact
     6241 gcagaaagat aagatggcgg aggcctacag tgagattggg atgaaaggcg agcgccggag
     6301 gggcaagggg cacgatggcc tttaccaggg tctcagtaca gccaccaagg acacctacga
     6361 cgcccttcac atgcaggccc tgccccctcg catcgattga gtttggacaa accacaacta
     6421 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     6481 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     6541 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtactt
     6601 aagaggggga gaccaaaggg aagcttcctt ttgccaccta gctgtccagg ggtgccttaa
     6661 aatggcaaac aaggtttgtt ttcttttcct gttttcatgc cttcctcttc catatccttg
     6721 tttcatatta atacatgtgt atagatccta aaaatctata cacatgtatt aataaagcct
     6781 gattctgccg cttctaggta tagaggccac ctgcaagata aatatttgat tcacaataac
     6841 taatcattct atggcaattg ataacaacaa atatatatat atatatatat atacgtatat
     6901 gtgtatatat atatatatat tcaggaaata atatattcta gaatatgtca cattctgtct
     6961 caggcatcca ttttctttat gatgccgttt gaggtggagt tttagtcagg tggtcagctt
     7021 ctcctttttt ttgccatctg ccctgtaagc atcctgctgg ggacccagat aggagtcatc
     7081 actctaggct gagaacatct gggcacacac cctaagcctc agcatgactc atcatgactc
     7141 agcattgctg tgcttgagcc agaaggtttg cttagaaggt tacacagaac cagaaggcgg
     7201 gggtggggca ctgaccccga caggggcctg gccagaactg ctcatgcttg gactatggga
     7261 ggtcactaat ggagacacac agaaatgtaa caggaactaa ggaaaaactg aagcttattt
     7321 aatcagagat gaggatgctg gaagggatag agggagctga gcttgtaaaa agtatagtaa
     7381 tcattcagca aatggttttg aagcacctgc tggatgctaa acactatttt cagtgcttga
     7441 atcataaata agaataaaac atgtatctta ttccccacaa gagtccaagt aaaaaataac
     7501 agttaattat aatgtgctct gtcccccagg ctggagtgca gtggcacgat ctcagctcac
     7561 tgcaacctcc gcctcccggg ttcaagcaat tctcctgcct cagccaccct aatagctggg
     7621 attacaggtg cacaccacca tgccaggcta atttttgtac tttttgtaga ggcagggtat
     7681 caccatgttg tccaagatgg tcttgaactc ctgagctcca agcagtccac ccacctcagc
     7741 ctcccaaagt gctgggatta caggtgtgag acaccatgcc cagattttcc atatttaata
     7801 gaggtattta tgggatgggg gaaaagaatg tttctctcac tgtggattat tttagagagt
     7861 ggagaatggt caagattttt ttaaaaatta agaaaacata agttggacct tgagaaatga
     7921 aaatttattt ttttgttgga ggatacccat tctctatctc ccatcagggc aagctgtaag
     7981 gaactggcta agacacagtg agacagagtg acttagtctt agaggcccca ctggtacaag
     8041 ctttcattaa aaaaagtcta accagctgca ttcgactttg actgcagcag ctggttagaa
     8101 ggttctactg gaggagggtc ccagcccatt gctaaattaa catcaggctc tgagactggc
     8161 agtatatctc taacagtggt tgatgctatc ttctggaact tgcctgctac attgagacca
     8221 ctgacccata cataggaagc ccatagctct gtcctgaact gttaggccac tggtccagag
     8281 agtgtgcatc tcctttgatc ctcataataa ccctatgaga tagacacaat tattactctt
     8341 actttataga tgatgatcct gaaaacatag gagtcaaggc acttgcccct agctgggggt
     8401 ataggggagc agtcccatgt agtagtagaa tgaaaaatgc tgctatgctg tgcctccccc
     8461 acctttccca tgtctgccct ctactcatgg tctatctctc ctggctcctg ggagtcatgg
     8521 actccaccca gcaccaccaa cctgacctaa ccacctatct gagcctgcca gcctataacc
     8581 catctgggcc ctgatagctg gtggccagcc ctgaccccac cccaccctcc ctggaacctc
     8641 tgatagacac atctggcaca ccagctcgca aagtcaccgt gagggtcttg tgtttgctga
     8701 gtcaaaattc cttgaaatcc aagtccttag agactcctgc tcccaaattt acagtcatag
     8761 acttcttcat ggctgtctcc tttatccaca gaatgattcc tttgcttcat tgccccatcc
     8821 atctgatcct cctcatcagt gcagcacagg gcccatgagc agtagctgca gagtctcaca
     8881 taggtctggc actgcctctg acatgtccga ccttaggcaa atgcttgact cttctgagct
     8941 cagtcttgtc atggcaaaat aaagataata atagtgtttt tttatggagt tagcgtgagg
     9001 atggaaaaca atagcaaaat tgattagact ataaaaggtc tcaacaaata gtagtagatt
     9061 ttatcgtcca ttaatccttc cctctcctct cttactcatc ccatcacgta tgcctcttaa
     9121 ttttccctta cctataataa gagttattcc tcttattata ttcttcttat agtgattctg
     9181 gatattaaag tgggaatgag gggcaggcca ctaacgaaga agatgtttct caaagaagcg
     9241 taaatacact tgcaaaggag gatgttttta gtagcaattt gtactgatgg tatggggcca
     9301 agagatatat cttagaggga gggctgaggg tttgaagtcc aactcctaag ccagtgccag
     9361 aagagccaag gacaggtacg gctgtcatca cttagacctc accctgtgga gccacaccct
     9421 agggttggcc aatctactcc caggagcagg gagggcagga gccagggctg ggcataaaag
     9481 tcagggcaga gccatctatt gcttacattt gcttctgaca caactgtgtt cactagcaac
     9541 ctcaaacaga caccatggtg catctgactc ctgaggagaa gtctgccgtt actgccctgt
     9601 ggggcaaggt gaacgtggat gaagttggtg gtgaggccct gggcaggttg gtatcaaggt
     9661 tacaagacag gtttaaggag accaatagaa actgggcatg tggagacaga gaagactctt
     9721 gggtttctga taggcactga ctctctctgc ctattggtct attttcccac ccttaggctg
     9781 ctggtggtct acccttggac ccagaggttc tttgagtcct ttggggatct gtccactcct
     9841 gatgctgtta tgggcaaccc taaggtgaag gctcatggca agaaagtgct cggtgccttt
     9901 agtgatggcc tggctcacct ggacaacctc aagggcacct ttgccacact gagtgagctg
     9961 cactgtgaca agctgcacgt ggatcctgag aacttcaggg tgagtctatg ggacgcttga
    10021 tgttttcttt ccccttcttt tctatggtta agttcatgtc ataggaaggg gataagtaac
    10081 agggtacagt ttagaatggg aaacagacga atgattgcat cagtgtggaa gtctcaggat
    10141 cgttttagtt tcttttattt gctgttcata acaattgttt tcttttgttt aattcttgct
    10201 ttcttttttt ttcttctccg caatttttac tattatactt aatgccttaa cattgtgtat
    10261 aacaaaagga aatatctctg agatacatta agtaacttaa aaaaaaactt tacacagtct
    10321 gcctagtaca ttactatttg gaatatatgt gtgcttattt gcatattcat aatctcccta
    10381 ctttattttc ttttattttt aattgataca taatcattat acatatttat gggttaaagt
    10441 gtaatgtttt aatatgtgta cacatattga ccaaatcagg gtaattttgc atttgtaatt
    10501 ttaaaaaatg ctttcttctt ttaatatact tttttgttta tcttatttct aatactttcc
    10561 ctaatctctt tctttcaggg caataatgat acaatgtatc atgcctcttt gcaccattct
    10621 aaagaataac agtgataatt tctgggttaa ggcaatagca atatctctgc atataaatat
    10681 ttctgcatat aaattgtaac tgatgtaaga ggtttcatat tgctaatagc agctacaatc
    10741 cagctaccat tctgctttta ttttatggtt gggataaggc tggattattc tgagtccaag
    10801 ctaggccctt ttgctaatca tgttcatacc tcttatcttc ctcccacagc tcctgggcaa
    10861 cgtgctggtc tgtgtgctgg cccatcactt tggcaaagaa ttcaccccac cagtgcaggc
    10921 tgcctatcag aaagtggtgg ctggtgtggc taatgccctg gcccacaagt atcactaagc
    10981 tcgctttctt gctgtccaat ttctattaaa ggttcctttg ttccctaagt ccaactacta
    11041 aactggggga tattatgaag ggccttgagc atctggattc tgcctaataa aaaacattta
    11101 ttttcattgc aatgatgtat ttaaattatt tctgaatatt ttactaaaaa gggaatgtgg
    11161 gaggtcagtg catttaaaac ataaagaaat gaagagctag ttcaaacctt gggaaaatac
    11221 actatatctt aaactccatg aaagaaggtg aggctgcaaa cagctaatgc acattggcaa
    11281 cagcccctga tgcatatgcc ttattcatcc ctcagaaaag gattcaagta gaggcttgat
    11341 ttggaggtta aagttttgct atgctgtatt ttacattact tattgtttta gctgtcctca
    11401 tgggccctcg agatgctacc aagttagccc aggataaggc tgttttgtta ttttctttag
    11461 tttttcaaat gtacgggctg gccacattgc gaattaaaag ccggagctgt aattatttat
    11521 aggcaataat gataaatggt taaaataata aaattttcta tactggtcct attattttgt
    11581 cgcatgattt ttggggtttt ttcaacattt cttataataa cttttttgtt tgttaactta
    11641 tcaacaaact ttcaaagcag actttaagcc aacatccgaa aagtgctttc atgaaaattt
    11701 ggtaaatatt gattcacttt aggtaatgaa atgtgataag tgaataaaac attattaagt
    11761 ggtcctattg atttgtccga cactgtatag ggcccagaga cggagtcact gccaaccgag
    11821 acggtcatag ctgtttcctg tgtgccgctt cctcgctcac tgactcgctg cgctcggtcg
    11881 ttactgacca tttaaatcat acctgacctc catagcagaa agtcaaaagc ctccgaccgg
    11941 aggcttttga cttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc
    12001 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag
    12061 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    12121 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    12181 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagtttac gccccgaaga
    12241 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    12301 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    12361 gtactcacca gtcacagaaa agcatctcac ggatggcatg acagtaagag aattatgcag
    12421 tgctgccata accatgagtg ataacactgc ggccaactta cttctggcaa cgatcggagg
    12481 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    12541 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    12601 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    12661 gcaacaatta atagactgga tggaggcgga taaagttgca ggatcacttc tgcgctcggc
    12721 cctcccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtcccgcgg
    12781 tatcattgca gcactggggc cagatggtaa gccctcccgc atcgtagtta tctacacgac
    12841 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    12901 gattaagcat tggtaagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat
    12961 cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc
    13021 agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg
    13081 tttcggtgat gacggtgaaa acctctgatg agggcccaaa
//
