LOCUS       pMApra-Ptac        9827 bp    DNA     circular UNA 14-MAR-2024
DEFINITION  pMMB207 with apramycin resistance (pMJG120).
ACCESSION   urn.local...hc-hh5gmuy
VERSION     urn.local...hc-hh5gmuy
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             1..837
                     /standard_name="RepA"
     CDS             728..1675
                     /standard_name="rep"
     CDS             complement(2101..3045)
                     /standard_name="LacIq"
     misc_feature    3413..3469
                     /standard_name="MCS"
     CDS             4953..5765
                     /gene="ApraR"
                     /product="Apramycin resistance gene aac(3) IV"
                     /standard_name="ApraR"
     CDS             7188..9314
                     /standard_name="MobA"
ORIGIN      
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgaatgc catgtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgcaagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccag
     1861 atgtatgctc ttctgctccc gaacgccagc aagacgtagc ccagcgcgtc ggccagcttg
     1921 caattcgcgc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1981 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2041 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     2101 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgtg gtttgcccca gcaggcgaaa
     2161 atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta
     2221 tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc
     2281 gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag
     2341 catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat
     2401 cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga
     2461 gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg
     2521 ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg
     2581 gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc
     2641 atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt
     2701 gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct
     2761 ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag
     2821 ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc
     2881 cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt
     2941 cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc
     3001 atactctgcg acatcgtata acgttactgg tttcacattc accaccctga attgactctc
     3061 ttccgggcgc tatcatgcca taccgcgaaa ggttttgcac cattcgatgg tgtcaacgta
     3121 aatgccgctt cgccttcgcg cgcgaattgc aagctgatcc gggcttatcg actgcacggt
     3181 gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg tgcaggtcgt
     3241 aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa tgttttttgc
     3301 gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa ttaatcatcg
     3361 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagaattcga
     3421 gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg gctgttttgg
     3481 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     3541 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     3601 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     3661 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3721 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     3781 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc
     3841 aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt
     3901 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3961 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4021 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4081 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4141 ggtaagatcc ttgagagttt tcgccccgaa gaacgtgatg tccggcggtg cttttgccgt
     4201 tacgcaccac cccgtcagta gctgaacagg agggacagct gatagaaaca gaagccactg
     4261 gagcaagcac ctcaaaaaca ccatcataca ctaaatcagt aagttggcag catcacccga
     4321 cgcactttgc gccgaataaa tacctgtgac ggaagatcac ttcgcagaat aaataaatcc
     4381 tggtgtccct gttgataccg ggaagccctg ggccaacttt tggcgaaaat gagacgttga
     4441 tcggcacgta agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt
     4501 ttttgagtta tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     4561 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     4621 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttatcaag
     4681 gcccgatcct tggagccctt gccctcccgc acgatgatcg tgccgtgatc gaaatccaga
     4741 tccttgaccc gcagttgcaa accctcactg atccggctca cggtaactga tgccgtattt
     4801 gcagtaccag cgtacggccc acagaatgat gtcacgctga aaatgccggc ctttgaatgg
     4861 gttcatgtgc agctccatca gcaaaagggg atgataagtt tatcaccacc gactatttgc
     4921 aacagtgccg ttgatcgtgc tatgatcgac tgatgtcatc agcggtggag tgcaatgtcg
     4981 tgcaatacga atggcgaaaa gccgagctca tcggtcagct tctcaacctt ggggttaccc
     5041 ccggcggtgt gctgctggtc cacagctcct tccgtagcgt ccggcccctc gaagatgggc
     5101 cacttggact gatcgaggcc ctgcgtgctg cgctgggtcc gggagggacg ctcgtcatgc
     5161 cctcgtggtc aggtctggac gacgagccgt tcgatcctgc cacgtcgccc gttacaccgg
     5221 accttggagt tgtctctgac acattctggc gcctgccaaa tgtaaagcgc agcgcccatc
     5281 catttgcctt tgcggcagcg gggccacagg cagagcagat catctctgat ccattgcccc
     5341 tgccacctca ctcgcctgca agcccggtcg cccgtgtcca tgaactcgat gggcaggtac
     5401 ttctcctcgg cgtgggacac gatgccaaca cgacgctgca tcttgccgag ttgatggcaa
     5461 aggttcccta tggggtgccg agacactgca ccattcttca ggatggcaag ttggtacgcg
     5521 tcgattatct cgagaatgac cactgctgtg agcgctttgc cttggcggac aggtggctca
     5581 aggagaagag ccttcagaag gaaggtccag tcggtcatgc ctttgctcgg ttgatccgct
     5641 cccgcgacat tgtggcgaca gccctgggtc aactgggccg agatccgttg atcttcctgc
     5701 atccgccaga gggcgggatg cgaagaatgc gatgccgctc gccagtcgat tggctgagct
     5761 catgaaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     5821 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     5881 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     5941 ggcggggcgt aattttttta aggcagttat tgtcttcaaa ttcccgttgc acatagcccg
     6001 gcaattcctt tccctgctct gccataagcg cagcgaatgc cgggtaatac tcgtcaacga
     6061 tctgatagag aagggtttgc tcgggtcggt ggctctggta acgaccagta tcccgatccc
     6121 ggctggccgt cctggccgcc acatgaggca tgttccgcgt ccttgcaata ctgtgtttac
     6181 atacagtcta tcgcttagcg gaaagttctt ttaccctcag ccgaaatgcc tgccgttgct
     6241 agacattgcc agccagtgcc cgtcactccc gtactaactg tcacgaaccc ctgcaataac
     6301 tgtcacgccc ccctgcaata actgtcacga acccctgcaa taactgtcac gcccccaaac
     6361 ctgcaaaccc agcaggggcg ggggctggcg gggtgttgga aaaatccatc catgattatc
     6421 taagaataat ccactaggcg cggttatcag cgcccttgtg gggcgctgct gcccttgccc
     6481 aatatgcccg gccagaggcc ggatagctgg tctattcgct gcgctaggct acacaccgcc
     6541 ccaccgctgc gcggcagggg gaaaggcggg caaagcccgc taaaccccac accaaacccc
     6601 gcagaaatac gctggagcgc ttttagccgc tttagcggcc tttcccccta cccgaagggt
     6661 gggggcgcgt gtgcagcccc gcagggcctg tctcggtcga tcattcagcc cggctcatcc
     6721 ttctggcgtg gcggcagacc gaacaaggcg cggtcgtggt cgcgttcaag gtacgcatcc
     6781 attgccgcca tgagccgatc ctccggccac tcgctgctgt tcaccttggc caaaatcatg
     6841 gcccccacca gcaccttgcg ccttgtttcg ttcttgcgct cttgctgctg ttcccttgcc
     6901 cgctcccgct gaatttcggc attgattcgc gctcgttgtt cttcgagctt ggccagccga
     6961 tccgccgcct tgttgctccc cttaaccatc ttgacacccc attgttaatg tgctgtctcg
     7021 taggctatca tggaggcaca gcggcggcaa tcccgaccct actttgtagg ggagggcgca
     7081 cttaccggtt tctcttcgag aaactggcct aacggccacc cttcgggcgg tgcgctctcc
     7141 gagggccatt gcatggagcc gaaaagcaaa agcaacagcg aggcagcatg gcgatttatc
     7201 accttacggc gaaaaccggc agcaggtcgg gcggccaatc ggccagggcc aaggccgact
     7261 acatccagcg cgaaggcaag tatgcccgcg acatggatga agtcttgcac gccgaatccg
     7321 ggcacatgcc ggagttcgtc gagcggcccg ccgactactg ggatgctgcc gacctgtatg
     7381 aacgcgccaa tgggcggctg ttcaaggagg tcgaatttgc cctgccggtc gagctgaccc
     7441 tcgaccagca gaaggcgctg gcgtccgagt tcgcccagca cctgaccggt gccgagcgcc
     7501 tgccgtatac gctggccatc catgccggtg gcggcgagaa cccgcactgc cacctgatga
     7561 tctccgagcg gatcaatgac ggcatcgagc ggcccgccgc tcagtggttc aagcggtaca
     7621 acggcaagac cccggagaag ggcggggcac agaagaccga agcgctcaag cccaaggcat
     7681 ggcttgagca gacccgcgag gcatgggccg accatgccaa ccgggcatta gagcgggctg
     7741 gccacgacgc ccgcattgac cacagaacac ttgaggcgca gggcatcgag cgcctgcccg
     7801 gtgttcacct ggggccgaac gtggtggaga tggaaggccg gggcatccgc accgaccggg
     7861 cagacgtggc cctgaacatc gacaccgcca acgcccagat catcgactta caggaatacc
     7921 gggaggcaat agaccatgaa cgcaatcgac agagtgaaga aatccagagg catcaacgag
     7981 ttagcggagc agatcgaacc gctggcccag agcatggcga cactggccga cgaagcccgg
     8041 caggtcatga gccagaccaa gcaggccagc gaggcgcagg cggcggagtg gctgaaagcc
     8101 cagcgccaga caggggcggc atgggtggag ctggccaaag agttgcggga ggtagccgcc
     8161 gaggtgagca gcgccgcgca gagcgcccgg agcgcgtcgc gggggtggca ctggaagcta
     8221 tggctaaccg tgatgctggc ttccatgatg cctacggtgg tgctgctgat cgcatcgttg
     8281 ctcttgctcg acctgacgcc actgacaacc gaggacggct cgatctggct gcgcttggtg
     8341 gcccgatgaa gaacgacagg actttgcagg ccataggccg acagctcaag gccatgggct
     8401 gtgagcgctt cgatatcggc gtcagggacg cacccaccgg ccagatgatg aaccgggaat
     8461 ggtcagccgc cgaagtgctc cagaacacgc catggctcaa gcggatgaat gcccagggca
     8521 atgacgtgta tatcaggccc gccgagcagg agcggcatgg tctggtgctg gtggacgacc
     8581 tcagcgagtt tgacctggat gacatgaaag ccgagggccg ggagcctgcc ctggtagtgg
     8641 aaaccagccc gaagaactat caggcatggg tcaaggtggc cgacgccgca ggcggtgaac
     8701 ttcgggggca gattgcccgg acgctggcca gcgagtacga cgccgacccg gccagcgccg
     8761 acagccgcca ctatggccgc ttggcgggct tcaccaaccg caaggacaag cacaccaccc
     8821 gcgccggtta tcagccgtgg gtgctgctgc gtgaatccaa gggcaagacc gccaccgctg
     8881 gcccggcgct ggtgcagcag gctggccagc agatcgagca ggcccagcgg cagcaggaga
     8941 aggcccgcag gctggccagc ctcgaactgc ccgagcggca gcttagccgc caccggcgca
     9001 cggcgctgga cgagtaccgc agcgagatgg ccgggctggt caagcgcttc ggtcatgacc
     9061 tcagcaagtg cgactttatc gccgcgcaga agctggccag ccggggccgc agtgccgagg
     9121 aaatcggcaa ggccatggcc gaggccagcc cagcgctggc agagcgcaag cccggccacg
     9181 aagcggatta catcgagcgc accgtcagca aggtcatggg tctgcccagc gtccagcttg
     9241 cgcgggccga gctggcacgg gcaccggcac cccgccagcg aggcatggac aggggcgggc
     9301 cagatttcag catgtagtgc ttgcgttggt actcacgcct gttatactat gagtactcac
     9361 gcacagaagg gggttttatg gaatacgaaa aaagcgcttc agggtcggtc tacctgatca
     9421 aaagtgacaa gggctattgg ttgcccggtg gctttggtta tacgtcaaac aaggccgagg
     9481 ctggccgctt ttcagtcgct gatatggcca gccttaacct tgacggctgc accttgtcct
     9541 tgttccgcga agacaagcct ttcggccccg gcaagtttct cggtgactga tatgaaagac
     9601 caaaaggaca agcagaccgg cgacctgctg gccagccctg acgctgtacg ccaagcgcga
     9661 tatgccgagc gcatgaaggc caaagggatg cgtcagcgca agttctggct gaccgacgac
     9721 gaatacgagg cgctgcgcga gtgcctggaa gaactcagag cggcgcaggg cgggggtagt
     9781 gaccccgcca gcgcctaacc accaactgcc tgcaaaggag gcaatca
//
