LOCUS       pMApra-Ptol       10180 bp    DNA     circular UNA 14-MAR-2024
DEFINITION  pMJG588
ACCESSION   urn.local...hh-hh5gmuy
VERSION     urn.local...hh-hh5gmuy
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             1..837
                     /standard_name="RepA"
     CDS             728..1675
                     /standard_name="rep"
     gene            complement(1954..2919)
                     /note="xylS839"
                     /standard_name="xylS839"
     CDS             complement(1954..2919)
                     /note="XylSArg41Pro. regulatory protein"
                     /standard_name="XylS CDS"
     misc_feature    3887..3921
                     /note="multiple cloning site"
                     /standard_name="multiple cloning site"
     regulatory      3929..3985
                     /standard_name="tR'"
     CDS             5306..6118
                     /gene="ApraR"
                     /product="Apramycin resistance gene aac(3) IV"
                     /standard_name="ApraR"
     CDS             7541..9667
                     /standard_name="MobA"
ORIGIN      
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgaatgc catgtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgcaagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccag
     1861 atgtatgctc ttctgctccc gaacgccagc aagacgtagc ccagcgcgtc ggccagcttg
     1921 caattcgcgc taacttacat taatcgttcg taatcaagcc acttcctttt tgcattgacg
     1981 cagggtgtcg gaaggcaact cgccgaacgc gctcctatag ttttcagcga agcgtcccaa
     2041 atgtaagaag ccgtagtcta gggctatctc agttatacta cgcacattgg cactgggatc
     2101 gttcaagcag gcgcggatgc tttcgagctt gcggttgcgg atgtagttct tcggcgtggt
     2161 gccggcatgc ttctcgaaca aattgtagag cgagcgtgga ctcatcatcg ccagctccgc
     2221 taaccgctca aggctgatat tccgtttgag attctcctca atgaattgaa cgactcgctc
     2281 gaaagacggg ttacctttgc tgaaaatttc acggctgaca ttgctgccca gcatttcgag
     2341 cagcttggaa gcgatgatcc ccgcatagtg ctcttggacc cgaggcatcg actttgtatg
     2401 ttccgcttcg tcacaaacta acccgagtag attgataaag ccatcgagtt gctggagatt
     2461 gtgtcgcgcg gcgaaacgga taccctccct cggcttgtgc caattgttgt cactgcatgc
     2521 ccgatcaagg accactgagg gcaatttaac gataaatttc tcgcaatctt ctgaataggt
     2581 caggtcggct tggtcatccg gattgagcag caatagttcg cccggcgcaa aatagtgctc
     2641 ctggccatgg ccacgccaca ggcaatggcc tttgagtatt atttgcagat gataacaggt
     2701 ctctaatcca ggcgagatta ccctcacgct accgccgtag ctgattcgac acaggtcgag
     2761 gcatccgaag attctgtggt gcagcctgcc tgccgggcgc ccgcccttgg gcaggcgaat
     2821 agagtgcgta ccgacatact ggttaacata atcggagact gcatagggct cggcgtggac
     2881 gaagatctga cttttctcgt tcaataagca aaaatccata gttcacggtt ctcttatttt
     2941 aatgtgggct gcttggtgtg atgtagaaag gcgccaagtc gatgaaaatg catctcgacg
     3001 tgatgcgtat acgggttacc cccattgcca cgttgcgcca tcctttttgc aatcagtgac
     3061 cacttttcca agcaaaaata acgccaagca gaacgaagac gttcttttta agaagcgaga
     3121 acaccagaag ttcgtgctgt cggggcatgc ggcgacgaat tggcggataa aggggatctg
     3181 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     3241 tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattacgtat ggcaatgaaa
     3301 gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa
     3361 actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac
     3421 atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt
     3481 attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta
     3541 aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgcatgg gaattagctt
     3601 gatctgacca acgaccggta gcggagctat ccaacggcgg tataccagga aaacacacag
     3661 caggtacatc agaacagtac catgactgaa gaacaaatag ttttttcctg atccataaag
     3721 cagaacggcc tgctccatga caaatctggc tccccaacta atgccccatg cagccagcat
     3781 aaccagcata aactgtccgg tttgataggg ataagtccag ccttgcaaga agcggataca
     3841 ggagtgcaaa aaatggctat ctctagaaag gcctacccct taggctggat ccacgtcgaa
     3901 ttcctgcaga agcttactag tggctgaatc ccgaaacagt tcgcaggtaa tagttagagc
     3961 ctgcataacg gtttcgggat tttttatagg tctccccatg cgagagtagg gaactgccag
     4021 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     4081 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
     4141 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
     4201 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
     4261 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4321 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4381 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4441 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4501 tccttgagag ttttcgcccc gaagaacgtg atgtccggcg gtgcttttgc cgttacgcac
     4561 caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca ctggagcaag
     4621 cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt
     4681 tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc
     4741 cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac
     4801 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
     4861 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     4921 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     4981 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttatc aaggcccgat
     5041 ccttggagcc cttgccctcc cgcacgatga tcgtgccgtg atcgaaatcc agatccttga
     5101 cccgcagttg caaaccctca ctgatccggc tcacggtaac tgatgccgta tttgcagtac
     5161 cagcgtacgg cccacagaat gatgtcacgc tgaaaatgcc ggcctttgaa tgggttcatg
     5221 tgcagctcca tcagcaaaag gggatgataa gtttatcacc accgactatt tgcaacagtg
     5281 ccgttgatcg tgctatgatc gactgatgtc atcagcggtg gagtgcaatg tcgtgcaata
     5341 cgaatggcga aaagccgagc tcatcggtca gcttctcaac cttggggtta cccccggcgg
     5401 tgtgctgctg gtccacagct ccttccgtag cgtccggccc ctcgaagatg ggccacttgg
     5461 actgatcgag gccctgcgtg ctgcgctggg tccgggaggg acgctcgtca tgccctcgtg
     5521 gtcaggtctg gacgacgagc cgttcgatcc tgccacgtcg cccgttacac cggaccttgg
     5581 agttgtctct gacacattct ggcgcctgcc aaatgtaaag cgcagcgccc atccatttgc
     5641 ctttgcggca gcggggccac aggcagagca gatcatctct gatccattgc ccctgccacc
     5701 tcactcgcct gcaagcccgg tcgcccgtgt ccatgaactc gatgggcagg tacttctcct
     5761 cggcgtggga cacgatgcca acacgacgct gcatcttgcc gagttgatgg caaaggttcc
     5821 ctatggggtg ccgagacact gcaccattct tcaggatggc aagttggtac gcgtcgatta
     5881 tctcgagaat gaccactgct gtgagcgctt tgccttggcg gacaggtggc tcaaggagaa
     5941 gagccttcag aaggaaggtc cagtcggtca tgcctttgct cggttgatcc gctcccgcga
     6001 cattgtggcg acagccctgg gtcaactggg ccgagatccg ttgatcttcc tgcatccgcc
     6061 agagggcggg atgcgaagaa tgcgatgccg ctcgccagtc gattggctga gctcatgaaa
     6121 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
     6181 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
     6241 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
     6301 cgtaattttt ttaaggcagt tattgtcttc aaattcccgt tgcacatagc ccggcaattc
     6361 ctttccctgc tctgccataa gcgcagcgaa tgccgggtaa tactcgtcaa cgatctgata
     6421 gagaagggtt tgctcgggtc ggtggctctg gtaacgacca gtatcccgat cccggctggc
     6481 cgtcctggcc gccacatgag gcatgttccg cgtccttgca atactgtgtt tacatacagt
     6541 ctatcgctta gcggaaagtt cttttaccct cagccgaaat gcctgccgtt gctagacatt
     6601 gccagccagt gcccgtcact cccgtactaa ctgtcacgaa cccctgcaat aactgtcacg
     6661 cccccctgca ataactgtca cgaacccctg caataactgt cacgccccca aacctgcaaa
     6721 cccagcaggg gcgggggctg gcggggtgtt ggaaaaatcc atccatgatt atctaagaat
     6781 aatccactag gcgcggttat cagcgccctt gtggggcgct gctgcccttg cccaatatgc
     6841 ccggccagag gccggatagc tggtctattc gctgcgctag gctacacacc gccccaccgc
     6901 tgcgcggcag ggggaaaggc gggcaaagcc cgctaaaccc cacaccaaac cccgcagaaa
     6961 tacgctggag cgcttttagc cgctttagcg gcctttcccc ctacccgaag ggtgggggcg
     7021 cgtgtgcagc cccgcagggc ctgtctcggt cgatcattca gcccggctca tccttctggc
     7081 gtggcggcag accgaacaag gcgcggtcgt ggtcgcgttc aaggtacgca tccattgccg
     7141 ccatgagccg atcctccggc cactcgctgc tgttcacctt ggccaaaatc atggccccca
     7201 ccagcacctt gcgccttgtt tcgttcttgc gctcttgctg ctgttccctt gcccgctccc
     7261 gctgaatttc ggcattgatt cgcgctcgtt gttcttcgag cttggccagc cgatccgccg
     7321 ccttgttgct ccccttaacc atcttgacac cccattgtta atgtgctgtc tcgtaggcta
     7381 tcatggaggc acagcggcgg caatcccgac cctactttgt aggggagggc gcacttaccg
     7441 gtttctcttc gagaaactgg cctaacggcc acccttcggg cggtgcgctc tccgagggcc
     7501 attgcatgga gccgaaaagc aaaagcaaca gcgaggcagc atggcgattt atcaccttac
     7561 ggcgaaaacc ggcagcaggt cgggcggcca atcggccagg gccaaggccg actacatcca
     7621 gcgcgaaggc aagtatgccc gcgacatgga tgaagtcttg cacgccgaat ccgggcacat
     7681 gccggagttc gtcgagcggc ccgccgacta ctgggatgct gccgacctgt atgaacgcgc
     7741 caatgggcgg ctgttcaagg aggtcgaatt tgccctgccg gtcgagctga ccctcgacca
     7801 gcagaaggcg ctggcgtccg agttcgccca gcacctgacc ggtgccgagc gcctgccgta
     7861 tacgctggcc atccatgccg gtggcggcga gaacccgcac tgccacctga tgatctccga
     7921 gcggatcaat gacggcatcg agcggcccgc cgctcagtgg ttcaagcggt acaacggcaa
     7981 gaccccggag aagggcgggg cacagaagac cgaagcgctc aagcccaagg catggcttga
     8041 gcagacccgc gaggcatggg ccgaccatgc caaccgggca ttagagcggg ctggccacga
     8101 cgcccgcatt gaccacagaa cacttgaggc gcagggcatc gagcgcctgc ccggtgttca
     8161 cctggggccg aacgtggtgg agatggaagg ccggggcatc cgcaccgacc gggcagacgt
     8221 ggccctgaac atcgacaccg ccaacgccca gatcatcgac ttacaggaat accgggaggc
     8281 aatagaccat gaacgcaatc gacagagtga agaaatccag aggcatcaac gagttagcgg
     8341 agcagatcga accgctggcc cagagcatgg cgacactggc cgacgaagcc cggcaggtca
     8401 tgagccagac caagcaggcc agcgaggcgc aggcggcgga gtggctgaaa gcccagcgcc
     8461 agacaggggc ggcatgggtg gagctggcca aagagttgcg ggaggtagcc gccgaggtga
     8521 gcagcgccgc gcagagcgcc cggagcgcgt cgcgggggtg gcactggaag ctatggctaa
     8581 ccgtgatgct ggcttccatg atgcctacgg tggtgctgct gatcgcatcg ttgctcttgc
     8641 tcgacctgac gccactgaca accgaggacg gctcgatctg gctgcgcttg gtggcccgat
     8701 gaagaacgac aggactttgc aggccatagg ccgacagctc aaggccatgg gctgtgagcg
     8761 cttcgatatc ggcgtcaggg acgcacccac cggccagatg atgaaccggg aatggtcagc
     8821 cgccgaagtg ctccagaaca cgccatggct caagcggatg aatgcccagg gcaatgacgt
     8881 gtatatcagg cccgccgagc aggagcggca tggtctggtg ctggtggacg acctcagcga
     8941 gtttgacctg gatgacatga aagccgaggg ccgggagcct gccctggtag tggaaaccag
     9001 cccgaagaac tatcaggcat gggtcaaggt ggccgacgcc gcaggcggtg aacttcgggg
     9061 gcagattgcc cggacgctgg ccagcgagta cgacgccgac ccggccagcg ccgacagccg
     9121 ccactatggc cgcttggcgg gcttcaccaa ccgcaaggac aagcacacca cccgcgccgg
     9181 ttatcagccg tgggtgctgc tgcgtgaatc caagggcaag accgccaccg ctggcccggc
     9241 gctggtgcag caggctggcc agcagatcga gcaggcccag cggcagcagg agaaggcccg
     9301 caggctggcc agcctcgaac tgcccgagcg gcagcttagc cgccaccggc gcacggcgct
     9361 ggacgagtac cgcagcgaga tggccgggct ggtcaagcgc ttcggtcatg acctcagcaa
     9421 gtgcgacttt atcgccgcgc agaagctggc cagccggggc cgcagtgccg aggaaatcgg
     9481 caaggccatg gccgaggcca gcccagcgct ggcagagcgc aagcccggcc acgaagcgga
     9541 ttacatcgag cgcaccgtca gcaaggtcat gggtctgccc agcgtccagc ttgcgcgggc
     9601 cgagctggca cgggcaccgg caccccgcca gcgaggcatg gacaggggcg ggccagattt
     9661 cagcatgtag tgcttgcgtt ggtactcacg cctgttatac tatgagtact cacgcacaga
     9721 agggggtttt atggaatacg aaaaaagcgc ttcagggtcg gtctacctga tcaaaagtga
     9781 caagggctat tggttgcccg gtggctttgg ttatacgtca aacaaggccg aggctggccg
     9841 cttttcagtc gctgatatgg ccagccttaa ccttgacggc tgcaccttgt ccttgttccg
     9901 cgaagacaag cctttcggcc ccggcaagtt tctcggtgac tgatatgaaa gaccaaaagg
     9961 acaagcagac cggcgacctg ctggccagcc ctgacgctgt acgccaagcg cgatatgccg
    10021 agcgcatgaa ggccaaaggg atgcgtcagc gcaagttctg gctgaccgac gacgaatacg
    10081 aggcgctgcg cgagtgcctg gaagaactca gagcggcgca gggcgggggt agtgaccccg
    10141 ccagcgccta accaccaact gcctgcaaag gaggcaatca
//
