LOCUS       pMApra-Ptet2        8940 bp    DNA     circular UNA 14-MAR-2024
DEFINITION  pMJG599 (pMMB-apra-tet-2x-tetO)
ACCESSION   urn.local...hj-hh5gmuy
VERSION     urn.local...hj-hh5gmuy
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             1..837
                     /standard_name="RepA"
     CDS             728..1675
                     /standard_name="rep"
     CDS             complement(1946..2569)
                     /standard_name="TetR"
     regulatory      join(complement(2586),complement(2594..2599),
                     complement(2616..2621))
                     /standard_name="P-tetR2"
     regulatory      join(2589..2594,2612..2617,2624)
                     /standard_name="P-tetA"
     protein_bind    2594..2612
                     /standard_name="tetO1"
     regulatory      join(complement(2638..2643),complement(2614..2619),
                     complement(2606))
                     /standard_name="PtetR-1"
     protein_bind    2624..2642
                     /standard_name="tetO2"
     misc_feature    2652..2681
                     /note="multiple cloning site"
                     /standard_name="multiple cloning site"
     regulatory      2689..2745
                     /standard_name="tR'"
     CDS             4066..4878
                     /gene="ApraR"
                     /product="Apramycin resistance gene aac(3) IV"
                     /standard_name="ApraR"
     CDS             6301..8427
                     /standard_name="MobA"
ORIGIN      
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgaatgc catgtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgcaagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccag
     1861 atgtatgctc ttctgctccc gaacgccagc aagacgtagc ccagcgcgtc ggccagcttg
     1921 caattcgcgc taacttacat taattttaag acccactttc acatttaagt tgtttttcta
     1981 atccgcatat gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa
     2041 ataattcgat agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt
     2101 cttctttagc gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca
     2161 cagcgctgag tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta
     2221 attgattttc gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg
     2281 ctccatcgcg atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat
     2341 cttgccagct ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa
     2401 tggctaaggc gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct
     2461 ctacacctag cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa
     2521 gcagctctaa tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct
     2581 aatttttgtt gacactctat cgttgataga gttattttac cactccctat cagtgataga
     2641 gaaaatctag agacgtcgaa ttcctgcaga agcttactag tggctgaatc ccgaaacagt
     2701 tcgcaggtaa tagttagagc ctgcataacg gtttcgggat tttttatagg tctccccatg
     2761 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     2821 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
     2881 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
     2941 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
     3001 caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3061 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3121 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     3181 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     3241 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtg atgtccggcg
     3301 gtgcttttgc cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa
     3361 acagaagcca ctggagcaag cacctcaaaa acaccatcat acactaaatc agtaagttgg
     3421 cagcatcacc cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag
     3481 aataaataaa tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa
     3541 aatgagacgt tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
     3601 taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga
     3661 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     3721 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     3781 cctttttatc aaggcccgat ccttggagcc cttgccctcc cgcacgatga tcgtgccgtg
     3841 atcgaaatcc agatccttga cccgcagttg caaaccctca ctgatccggc tcacggtaac
     3901 tgatgccgta tttgcagtac cagcgtacgg cccacagaat gatgtcacgc tgaaaatgcc
     3961 ggcctttgaa tgggttcatg tgcagctcca tcagcaaaag gggatgataa gtttatcacc
     4021 accgactatt tgcaacagtg ccgttgatcg tgctatgatc gactgatgtc atcagcggtg
     4081 gagtgcaatg tcgtgcaata cgaatggcga aaagccgagc tcatcggtca gcttctcaac
     4141 cttggggtta cccccggcgg tgtgctgctg gtccacagct ccttccgtag cgtccggccc
     4201 ctcgaagatg ggccacttgg actgatcgag gccctgcgtg ctgcgctggg tccgggaggg
     4261 acgctcgtca tgccctcgtg gtcaggtctg gacgacgagc cgttcgatcc tgccacgtcg
     4321 cccgttacac cggaccttgg agttgtctct gacacattct ggcgcctgcc aaatgtaaag
     4381 cgcagcgccc atccatttgc ctttgcggca gcggggccac aggcagagca gatcatctct
     4441 gatccattgc ccctgccacc tcactcgcct gcaagcccgg tcgcccgtgt ccatgaactc
     4501 gatgggcagg tacttctcct cggcgtggga cacgatgcca acacgacgct gcatcttgcc
     4561 gagttgatgg caaaggttcc ctatggggtg ccgagacact gcaccattct tcaggatggc
     4621 aagttggtac gcgtcgatta tctcgagaat gaccactgct gtgagcgctt tgccttggcg
     4681 gacaggtggc tcaaggagaa gagccttcag aaggaaggtc cagtcggtca tgcctttgct
     4741 cggttgatcc gctcccgcga cattgtggcg acagccctgg gtcaactggg ccgagatccg
     4801 ttgatcttcc tgcatccgcc agagggcggg atgcgaagaa tgcgatgccg ctcgccagtc
     4861 gattggctga gctcatgaaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     4921 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     4981 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     5041 cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattgtcttc aaattcccgt
     5101 tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa tgccgggtaa
     5161 tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg gtaacgacca
     5221 gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg cgtccttgca
     5281 atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct cagccgaaat
     5341 gcctgccgtt gctagacatt gccagccagt gcccgtcact cccgtactaa ctgtcacgaa
     5401 cccctgcaat aactgtcacg cccccctgca ataactgtca cgaacccctg caataactgt
     5461 cacgccccca aacctgcaaa cccagcaggg gcgggggctg gcggggtgtt ggaaaaatcc
     5521 atccatgatt atctaagaat aatccactag gcgcggttat cagcgccctt gtggggcgct
     5581 gctgcccttg cccaatatgc ccggccagag gccggatagc tggtctattc gctgcgctag
     5641 gctacacacc gccccaccgc tgcgcggcag ggggaaaggc gggcaaagcc cgctaaaccc
     5701 cacaccaaac cccgcagaaa tacgctggag cgcttttagc cgctttagcg gcctttcccc
     5761 ctacccgaag ggtgggggcg cgtgtgcagc cccgcagggc ctgtctcggt cgatcattca
     5821 gcccggctca tccttctggc gtggcggcag accgaacaag gcgcggtcgt ggtcgcgttc
     5881 aaggtacgca tccattgccg ccatgagccg atcctccggc cactcgctgc tgttcacctt
     5941 ggccaaaatc atggccccca ccagcacctt gcgccttgtt tcgttcttgc gctcttgctg
     6001 ctgttccctt gcccgctccc gctgaatttc ggcattgatt cgcgctcgtt gttcttcgag
     6061 cttggccagc cgatccgccg ccttgttgct ccccttaacc atcttgacac cccattgtta
     6121 atgtgctgtc tcgtaggcta tcatggaggc acagcggcgg caatcccgac cctactttgt
     6181 aggggagggc gcacttaccg gtttctcttc gagaaactgg cctaacggcc acccttcggg
     6241 cggtgcgctc tccgagggcc attgcatgga gccgaaaagc aaaagcaaca gcgaggcagc
     6301 atggcgattt atcaccttac ggcgaaaacc ggcagcaggt cgggcggcca atcggccagg
     6361 gccaaggccg actacatcca gcgcgaaggc aagtatgccc gcgacatgga tgaagtcttg
     6421 cacgccgaat ccgggcacat gccggagttc gtcgagcggc ccgccgacta ctgggatgct
     6481 gccgacctgt atgaacgcgc caatgggcgg ctgttcaagg aggtcgaatt tgccctgccg
     6541 gtcgagctga ccctcgacca gcagaaggcg ctggcgtccg agttcgccca gcacctgacc
     6601 ggtgccgagc gcctgccgta tacgctggcc atccatgccg gtggcggcga gaacccgcac
     6661 tgccacctga tgatctccga gcggatcaat gacggcatcg agcggcccgc cgctcagtgg
     6721 ttcaagcggt acaacggcaa gaccccggag aagggcgggg cacagaagac cgaagcgctc
     6781 aagcccaagg catggcttga gcagacccgc gaggcatggg ccgaccatgc caaccgggca
     6841 ttagagcggg ctggccacga cgcccgcatt gaccacagaa cacttgaggc gcagggcatc
     6901 gagcgcctgc ccggtgttca cctggggccg aacgtggtgg agatggaagg ccggggcatc
     6961 cgcaccgacc gggcagacgt ggccctgaac atcgacaccg ccaacgccca gatcatcgac
     7021 ttacaggaat accgggaggc aatagaccat gaacgcaatc gacagagtga agaaatccag
     7081 aggcatcaac gagttagcgg agcagatcga accgctggcc cagagcatgg cgacactggc
     7141 cgacgaagcc cggcaggtca tgagccagac caagcaggcc agcgaggcgc aggcggcgga
     7201 gtggctgaaa gcccagcgcc agacaggggc ggcatgggtg gagctggcca aagagttgcg
     7261 ggaggtagcc gccgaggtga gcagcgccgc gcagagcgcc cggagcgcgt cgcgggggtg
     7321 gcactggaag ctatggctaa ccgtgatgct ggcttccatg atgcctacgg tggtgctgct
     7381 gatcgcatcg ttgctcttgc tcgacctgac gccactgaca accgaggacg gctcgatctg
     7441 gctgcgcttg gtggcccgat gaagaacgac aggactttgc aggccatagg ccgacagctc
     7501 aaggccatgg gctgtgagcg cttcgatatc ggcgtcaggg acgcacccac cggccagatg
     7561 atgaaccggg aatggtcagc cgccgaagtg ctccagaaca cgccatggct caagcggatg
     7621 aatgcccagg gcaatgacgt gtatatcagg cccgccgagc aggagcggca tggtctggtg
     7681 ctggtggacg acctcagcga gtttgacctg gatgacatga aagccgaggg ccgggagcct
     7741 gccctggtag tggaaaccag cccgaagaac tatcaggcat gggtcaaggt ggccgacgcc
     7801 gcaggcggtg aacttcgggg gcagattgcc cggacgctgg ccagcgagta cgacgccgac
     7861 ccggccagcg ccgacagccg ccactatggc cgcttggcgg gcttcaccaa ccgcaaggac
     7921 aagcacacca cccgcgccgg ttatcagccg tgggtgctgc tgcgtgaatc caagggcaag
     7981 accgccaccg ctggcccggc gctggtgcag caggctggcc agcagatcga gcaggcccag
     8041 cggcagcagg agaaggcccg caggctggcc agcctcgaac tgcccgagcg gcagcttagc
     8101 cgccaccggc gcacggcgct ggacgagtac cgcagcgaga tggccgggct ggtcaagcgc
     8161 ttcggtcatg acctcagcaa gtgcgacttt atcgccgcgc agaagctggc cagccggggc
     8221 cgcagtgccg aggaaatcgg caaggccatg gccgaggcca gcccagcgct ggcagagcgc
     8281 aagcccggcc acgaagcgga ttacatcgag cgcaccgtca gcaaggtcat gggtctgccc
     8341 agcgtccagc ttgcgcgggc cgagctggca cgggcaccgg caccccgcca gcgaggcatg
     8401 gacaggggcg ggccagattt cagcatgtag tgcttgcgtt ggtactcacg cctgttatac
     8461 tatgagtact cacgcacaga agggggtttt atggaatacg aaaaaagcgc ttcagggtcg
     8521 gtctacctga tcaaaagtga caagggctat tggttgcccg gtggctttgg ttatacgtca
     8581 aacaaggccg aggctggccg cttttcagtc gctgatatgg ccagccttaa ccttgacggc
     8641 tgcaccttgt ccttgttccg cgaagacaag cctttcggcc ccggcaagtt tctcggtgac
     8701 tgatatgaaa gaccaaaagg acaagcagac cggcgacctg ctggccagcc ctgacgctgt
     8761 acgccaagcg cgatatgccg agcgcatgaa ggccaaaggg atgcgtcagc gcaagttctg
     8821 gctgaccgac gacgaatacg aggcgctgcg cgagtgcctg gaagaactca gagcggcgca
     8881 gggcgggggt agtgaccccg ccagcgccta accaccaact gcctgcaaag gaggcaatca
//
