LOCUS       pMApra-Para        9532 bp    DNA     circular UNA 14-MAR-2024
DEFINITION  pMJG120 with araC-ParaBAD
ACCESSION   urn.local...he-hh5gmuy
VERSION     urn.local...he-hh5gmuy
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             1..837
                     /standard_name="RepA"
     CDS             728..1675
                     /standard_name="rep"
     CDS             complement(2055..2933)
                     /standard_name="araC"
     misc_feature    3238..3273
                     /note="multiple cloning site"
                     /standard_name="multiple cloning site"
     regulatory      3281..3337
                     /standard_name="tR'"
     CDS             4658..5470
                     /gene="ApraR"
                     /product="Apramycin resistance gene aac(3) IV"
                     /standard_name="ApraR CDS"
     CDS             6893..9019
                     /standard_name="MobA"
ORIGIN      
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgaatgc catgtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgcaagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccag
     1861 atgtatgctc ttctgctccc gaacgccagc aagacgtagc ccagcgcgtc ggccagcttg
     1921 caattcgcgc taacttacat taatctcgag gtcgacggta tcgatgcata atgtgcctgt
     1981 caaatggacg aagcagggat tctgcaaacc ctatgctact ccgtcaagcc gtcaattgtc
     2041 tgattcgtta ccaattatga caacttgacg gctacatcat tcactttttc ttcacaaccg
     2101 gcacggaact cgctcgggct ggccccggtg cattttttaa atacccgcga gaaatagagt
     2161 tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt ggtgctcaaa
     2221 agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct aatccctaac
     2281 tgctggcgga aaagatgtga cagacgcgac ggcgacaagc aaacatgctg tgcgacgctg
     2341 gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc ctcgcgtacc
     2401 cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg cagtaacaat
     2461 tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg cccggcgtta
     2521 atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg gcgaaagaac
     2581 cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc gcgcggacga
     2641 aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta gtgatgaatc
     2701 tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg tccctgattt
     2761 ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat tcccagcggt
     2821 cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc cgccaccaga
     2881 tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc catacttttc
     2941 atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca ttgccgtcac
     3001 tgcgtctttt actggctctt ctcgctaacc aaaccggtaa ccccgcttat taaaagcatt
     3061 ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt ctataatcac
     3121 ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc catagcattt
     3181 ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc tactgtttct
     3241 agagacgtcg aattcctgca gaagcttact agtggctgaa tcccgaaaca gttcgcaggt
     3301 aatagttaga gcctgcataa cggtttcggg attttttata ggtctcccca tgcgagagta
     3361 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     3421 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
     3481 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
     3541 gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
     3601 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     3661 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     3721 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     3781 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     3841 acagcggtaa gatccttgag agttttcgcc ccgaagaacg tgatgtccgg cggtgctttt
     3901 gccgttacgc accaccccgt cagtagctga acaggaggga cagctgatag aaacagaagc
     3961 cactggagca agcacctcaa aaacaccatc atacactaaa tcagtaagtt ggcagcatca
     4021 cccgacgcac tttgcgccga ataaatacct gtgacggaag atcacttcgc agaataaata
     4081 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
     4141 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
     4201 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
     4261 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
     4321 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
     4381 tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg tgatcgaaat
     4441 ccagatcctt gacccgcagt tgcaaaccct cactgatccg gctcacggta actgatgccg
     4501 tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg ccggcctttg
     4561 aatgggttca tgtgcagctc catcagcaaa aggggatgat aagtttatca ccaccgacta
     4621 tttgcaacag tgccgttgat cgtgctatga tcgactgatg tcatcagcgg tggagtgcaa
     4681 tgtcgtgcaa tacgaatggc gaaaagccga gctcatcggt cagcttctca accttggggt
     4741 tacccccggc ggtgtgctgc tggtccacag ctccttccgt agcgtccggc ccctcgaaga
     4801 tgggccactt ggactgatcg aggccctgcg tgctgcgctg ggtccgggag ggacgctcgt
     4861 catgccctcg tggtcaggtc tggacgacga gccgttcgat cctgccacgt cgcccgttac
     4921 accggacctt ggagttgtct ctgacacatt ctggcgcctg ccaaatgtaa agcgcagcgc
     4981 ccatccattt gcctttgcgg cagcggggcc acaggcagag cagatcatct ctgatccatt
     5041 gcccctgcca cctcactcgc ctgcaagccc ggtcgcccgt gtccatgaac tcgatgggca
     5101 ggtacttctc ctcggcgtgg gacacgatgc caacacgacg ctgcatcttg ccgagttgat
     5161 ggcaaaggtt ccctatgggg tgccgagaca ctgcaccatt cttcaggatg gcaagttggt
     5221 acgcgtcgat tatctcgaga atgaccactg ctgtgagcgc tttgccttgg cggacaggtg
     5281 gctcaaggag aagagccttc agaaggaagg tccagtcggt catgcctttg ctcggttgat
     5341 ccgctcccgc gacattgtgg cgacagccct gggtcaactg ggccgagatc cgttgatctt
     5401 cctgcatccg ccagagggcg ggatgcgaag aatgcgatgc cgctcgccag tcgattggct
     5461 gagctcatga aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     5521 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     5581 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     5641 ggcagggcgg ggcgtaattt ttttaaggca gttattgtct tcaaattccc gttgcacata
     5701 gcccggcaat tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc
     5761 aacgatctga tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg
     5821 atcccggctg gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg
     5881 tttacataca gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg
     5941 ttgctagaca ttgccagcca gtgcccgtca ctcccgtact aactgtcacg aacccctgca
     6001 ataactgtca cgcccccctg caataactgt cacgaacccc tgcaataact gtcacgcccc
     6061 caaacctgca aacccagcag gggcgggggc tggcggggtg ttggaaaaat ccatccatga
     6121 ttatctaaga ataatccact aggcgcggtt atcagcgccc ttgtggggcg ctgctgccct
     6181 tgcccaatat gcccggccag aggccggata gctggtctat tcgctgcgct aggctacaca
     6241 ccgccccacc gctgcgcggc agggggaaag gcgggcaaag cccgctaaac cccacaccaa
     6301 accccgcaga aatacgctgg agcgctttta gccgctttag cggcctttcc ccctacccga
     6361 agggtggggg cgcgtgtgca gccccgcagg gcctgtctcg gtcgatcatt cagcccggct
     6421 catccttctg gcgtggcggc agaccgaaca aggcgcggtc gtggtcgcgt tcaaggtacg
     6481 catccattgc cgccatgagc cgatcctccg gccactcgct gctgttcacc ttggccaaaa
     6541 tcatggcccc caccagcacc ttgcgccttg tttcgttctt gcgctcttgc tgctgttccc
     6601 ttgcccgctc ccgctgaatt tcggcattga ttcgcgctcg ttgttcttcg agcttggcca
     6661 gccgatccgc cgccttgttg ctccccttaa ccatcttgac accccattgt taatgtgctg
     6721 tctcgtaggc tatcatggag gcacagcggc ggcaatcccg accctacttt gtaggggagg
     6781 gcgcacttac cggtttctct tcgagaaact ggcctaacgg ccacccttcg ggcggtgcgc
     6841 tctccgaggg ccattgcatg gagccgaaaa gcaaaagcaa cagcgaggca gcatggcgat
     6901 ttatcacctt acggcgaaaa ccggcagcag gtcgggcggc caatcggcca gggccaaggc
     6961 cgactacatc cagcgcgaag gcaagtatgc ccgcgacatg gatgaagtct tgcacgccga
     7021 atccgggcac atgccggagt tcgtcgagcg gcccgccgac tactgggatg ctgccgacct
     7081 gtatgaacgc gccaatgggc ggctgttcaa ggaggtcgaa tttgccctgc cggtcgagct
     7141 gaccctcgac cagcagaagg cgctggcgtc cgagttcgcc cagcacctga ccggtgccga
     7201 gcgcctgccg tatacgctgg ccatccatgc cggtggcggc gagaacccgc actgccacct
     7261 gatgatctcc gagcggatca atgacggcat cgagcggccc gccgctcagt ggttcaagcg
     7321 gtacaacggc aagaccccgg agaagggcgg ggcacagaag accgaagcgc tcaagcccaa
     7381 ggcatggctt gagcagaccc gcgaggcatg ggccgaccat gccaaccggg cattagagcg
     7441 ggctggccac gacgcccgca ttgaccacag aacacttgag gcgcagggca tcgagcgcct
     7501 gcccggtgtt cacctggggc cgaacgtggt ggagatggaa ggccggggca tccgcaccga
     7561 ccgggcagac gtggccctga acatcgacac cgccaacgcc cagatcatcg acttacagga
     7621 ataccgggag gcaatagacc atgaacgcaa tcgacagagt gaagaaatcc agaggcatca
     7681 acgagttagc ggagcagatc gaaccgctgg cccagagcat ggcgacactg gccgacgaag
     7741 cccggcaggt catgagccag accaagcagg ccagcgaggc gcaggcggcg gagtggctga
     7801 aagcccagcg ccagacaggg gcggcatggg tggagctggc caaagagttg cgggaggtag
     7861 ccgccgaggt gagcagcgcc gcgcagagcg cccggagcgc gtcgcggggg tggcactgga
     7921 agctatggct aaccgtgatg ctggcttcca tgatgcctac ggtggtgctg ctgatcgcat
     7981 cgttgctctt gctcgacctg acgccactga caaccgagga cggctcgatc tggctgcgct
     8041 tggtggcccg atgaagaacg acaggacttt gcaggccata ggccgacagc tcaaggccat
     8101 gggctgtgag cgcttcgata tcggcgtcag ggacgcaccc accggccaga tgatgaaccg
     8161 ggaatggtca gccgccgaag tgctccagaa cacgccatgg ctcaagcgga tgaatgccca
     8221 gggcaatgac gtgtatatca ggcccgccga gcaggagcgg catggtctgg tgctggtgga
     8281 cgacctcagc gagtttgacc tggatgacat gaaagccgag ggccgggagc ctgccctggt
     8341 agtggaaacc agcccgaaga actatcaggc atgggtcaag gtggccgacg ccgcaggcgg
     8401 tgaacttcgg gggcagattg cccggacgct ggccagcgag tacgacgccg acccggccag
     8461 cgccgacagc cgccactatg gccgcttggc gggcttcacc aaccgcaagg acaagcacac
     8521 cacccgcgcc ggttatcagc cgtgggtgct gctgcgtgaa tccaagggca agaccgccac
     8581 cgctggcccg gcgctggtgc agcaggctgg ccagcagatc gagcaggccc agcggcagca
     8641 ggagaaggcc cgcaggctgg ccagcctcga actgcccgag cggcagctta gccgccaccg
     8701 gcgcacggcg ctggacgagt accgcagcga gatggccggg ctggtcaagc gcttcggtca
     8761 tgacctcagc aagtgcgact ttatcgccgc gcagaagctg gccagccggg gccgcagtgc
     8821 cgaggaaatc ggcaaggcca tggccgaggc cagcccagcg ctggcagagc gcaagcccgg
     8881 ccacgaagcg gattacatcg agcgcaccgt cagcaaggtc atgggtctgc ccagcgtcca
     8941 gcttgcgcgg gccgagctgg cacgggcacc ggcaccccgc cagcgaggca tggacagggg
     9001 cgggccagat ttcagcatgt agtgcttgcg ttggtactca cgcctgttat actatgagta
     9061 ctcacgcaca gaagggggtt ttatggaata cgaaaaaagc gcttcagggt cggtctacct
     9121 gatcaaaagt gacaagggct attggttgcc cggtggcttt ggttatacgt caaacaaggc
     9181 cgaggctggc cgcttttcag tcgctgatat ggccagcctt aaccttgacg gctgcacctt
     9241 gtccttgttc cgcgaagaca agcctttcgg ccccggcaag tttctcggtg actgatatga
     9301 aagaccaaaa ggacaagcag accggcgacc tgctggccag ccctgacgct gtacgccaag
     9361 cgcgatatgc cgagcgcatg aaggccaaag ggatgcgtca gcgcaagttc tggctgaccg
     9421 acgacgaata cgaggcgctg cgcgagtgcc tggaagaact cagagcggcg cagggcgggg
     9481 gtagtgaccc cgccagcgcc taaccaccaa ctgcctgcaa aggaggcaat ca
//
