LOCUS       pUC18T-mini-Tn7T-hygro-Ptet1                 5607 bp    DNA     circular UNA 09-FEB-2024
DEFINITION  pMJG607 (pUC18T-mini-Tn7T-hygro-TetR-PtetA-1xTetO-MCS.
ACCESSION   urn.local...hk-hh5gmuy
VERSION     urn.local...hk-hh5gmuy
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     CDS             82..132
                     /standard_name="bla(M) (fragment)"
     protein_bind    221..268
                     /standard_name="FRT"
     misc_feature    323..>339
                     /note="MCS; multiple cloning site"
                     /standard_name="MCS; multiple cloning site"
     CDS             complement(341..964)
                     /standard_name="TetR"
     regulatory      join(complement(981),complement(989..994),
                     complement(1011..1016))
                     /standard_name="P-tetR2"
     regulatory      join(984..989,1007..1012,1019)
                     /standard_name="P-tetA"
     misc_feature    989..1007
                     /standard_name="tetO1"
     regulatory      join(complement(1009..1014),complement(1001))
                     /note="Geneious type: promoter"
                     /standard_name="PtetR-1"
     misc_feature    1019..>1022
                     /standard_name="tetO2"
     misc_feature    1023..1078
                     /note="MCS; multiple cloning site"
                     /standard_name="MCS; multiple cloning site"
     mobile_element  1079..1244
                     /note="left end of Tn7"
                     /mobile_element_type="transposon:Tn7L"
                     /standard_name="left end of Tn7 mobile element"
     oriT            1396..1663
                     /standard_name="oriT"
     rep_origin      1684..2357
     gene            complement(2502..3362)
                     /gene="bla"
                     /standard_name="bla gene"
     CDS             complement(2502..3362)
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AOR07791.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /standard_name="bla CDS"
     mobile_element  3944..4142
                     /note="right end of Tn7"
                     /mobile_element_type="transposon:Tn7R"
                     /standard_name="right end of Tn7 mobile element"
     regulatory      4191..4285
                     /regulatory_class="terminator"
                     /note="bacteriophage lambda T0 terminator"
                     /standard_name="bacteriophage lambda T0 terminator
                     regulatory"
     regulatory      4382..4479
                     /regulatory_class="terminator"
                     /note="Escherichia coli rrnB T1 terminator"
                     /standard_name="Escherichia coli rrnB T1 terminator"
     misc_feature    4520..4567
                     /note="FRT; Flp recombinase target site"
                     /standard_name="FRT; Flp recombinase target site"
     gene            complement(4582..5580)
                     /standard_name="hygro cassette"
ORIGIN      
        1 ccggcttaat tccgcacggc cggtcgcgac acggcctgtc cgcaccgcgg atcaggcgtt
       61 gacgatgacg ggctggtcgg ccacgtcggg gagtcaggca actatggatg aacgaaatag
      121 acagatcgct gatcagcggc ccggaaacgt cctcgaaacg acgcatgtgt tcctcctggt
      181 tggtacaggt ggttgggggt gctcggctgt cgctggtgtt gaagttccta ttccgaagtt
      241 cctattctct agaaagtata ggaacttcag agcgcttttg aagctaattc gatcatgcat
      301 gagctcgaat tcccggggat ccatgcatga gctcactagt ttaagaccca ctttcacatt
      361 taagttgttt ttctaatccg catatgatca attcaaggcc gaataagaag gctggctctg
      421 caccttggtg atcaaataat tcgatagctt gtcgtaataa tggcggcata ctatcagtag
      481 taggtgtttc cctttcttct ttagcgactt gatgctcttg atcttccaat acgcaaccta
      541 aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt
      601 ggcataaaaa ggctaattga ttttcgagag tttcatactg tttttctgta ggccgtgtac
      661 ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt
      721 tattacgtaa aaaatcttgc cagctttccc cttctaaagg gcaaaagtga gtatggtgcc
      781 tatctaacat ctcaatggct aaggcgtcga gcaaagcccg cttatttttt acatgccaat
      841 acaatgtagg ctgctctaca cctagcttct gggcgagttt acgggttgtt aaaccttcga
      901 ttccgacctc attaagcagc tctaatgcgc tgttaatcac tttactttta tctaatctag
      961 acatcattaa ttcctaattt ttgttgacac tctatcgttg atagagttat tttaccactc
     1021 ccctgcagga attcctcgag aagcttgggc ccggtacctc gcgaaggcct tgcaggccaa
     1081 ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta
     1141 cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact
     1201 ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt
     1261 cctgttatgt ttgggcgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1321 gtattgggcg ctcttccgct tcctcgctca ctgacccgct gcgctcggtc gttcggctgc
     1381 ggcgagcggt atcagagctt atcggccagc ctcgcagagc aggattcccg ttgagcaccg
     1441 ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac
     1501 ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc
     1561 aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc
     1621 cgaagcaggg ttatgcagcg gaaagtatac cttaaggaat cccctgataa cgcaggaaag
     1681 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     1741 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc gagtcagagg
     1801 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     1861 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     1921 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     1981 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2041 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     2101 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     2161 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     2221 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     2281 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     2341 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     2401 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     2461 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     2521 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2581 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2641 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2701 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2761 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     2821 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     2881 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     2941 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     3001 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     3061 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     3121 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3181 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3241 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3301 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3361 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3421 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3481 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     3541 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     3601 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     3661 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     3721 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     3781 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     3841 tcggtgcggg cctcttcgct attacgccag ctagaggacc agccgcgtaa cctggcaaaa
     3901 tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa
     3961 gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat
     4021 agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg
     4081 gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt
     4141 ggggttcgag gtcgacggta tcgataagct agcttaatta gctgagcttg gactcctgtt
     4201 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
     4261 ccgggcgttt tttattggtg agaatccaag ctagactgcg atgagtggca gggcggggcg
     4321 taattttttt aaggcagtta ttggtgccct taaacgcctg gggtaatgac tctctagctt
     4381 gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     4441 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ctaggagctt gcggcccgga
     4501 cgatcgaatt ggggatcttg aagttcctat tccgaagttc ctattctcta gaaagtatag
     4561 gaacttcagc tagagggggc gtcaggcgcc gggggcggtg tccggcggcc cccagaggaa
     4621 ctgcgccagt tcctccggat cggtgaagcc ggagagatcc agcggggtct cctcgaacac
     4681 ctcgaagtcg tgcaggaagg tgaaggcgag cagttcgcgg gcgaagtcct cggtccgctt
     4741 ccactgcgcc ccgtcgagca gcgcggccag gatctcgcgg tcgccccgga aggcgttgag
     4801 atgcagttgc accaggctgt agcgggagtc tcccgcatag acgtcggtga agtcgacgat
     4861 cccggtgacc tcggtcgcgg ccaggtccac gaagatgttg gtcccgtgca ggtcgccgtg
     4921 gacgaaccgg ggttcgcggc cggccagcag cgtgtccacg tccggcagcc agtcctccag
     4981 gcggtccagc agccggggcg agaggtagcc ccacccgcgg tggtcctcga cggtcgccgc
     5041 gcggcgttcc cgcagcagtt ccgggaagac ctcggaatgg ggggtgagca cggtgttccc
     5101 ggtcagcggc accctgtgca gccggccgag cacccggccg agttcgcggg ccagggcgag
     5161 cagcgcgttc cggtcggtcg tgccgtccat cgcggaccgc caggtggtgc cggtcatccg
     5221 gctcatcacc aggtagggcc acggccaggc tccggtgccg ggccgcagct cgccgcggcc
     5281 gaggaggcgg ggcaccggca ccggggcgtc cgccaggacc gcgtacgcct ccgactccga
     5341 cgcgaggctc tccggaccgc accagtgctc gccgaacagc ttgatcaccg ggccgggctc
     5401 gccgaccagt acggggttgg tgctctcgcc gggcacccgc agcaccggcg gcaccggcag
     5461 cccgagctcc tccagggctc ggcgggccag cggctcccag aattcctggt cgttccgcag
     5521 gctcgcgtag gaatcatccg aatcaatacg gtcgagaagt aacagggatt cttgtgtcac
     5581 agcggacctc tattcacagg gtacggg
//
