LOCUS       pWH1266-Apra-sgRNA                 9199 bp    DNA     circular SYN 09-FEB-2024
DEFINITION  pYDE07.apramycin (pWH1266-pgRNA).
ACCESSION   KJ955629
VERSION     KJ955629.1  GI:685453761
KEYWORDS    .
SOURCE      Cloning vector pMU125 (unknown)
  ORGANISM  Cloning vector pMU125
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Bahl,C.D.
  TITLE     Sequencing commonly used, cryptic plasmids from Acinetobacter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9445)
  AUTHORS   Bahl,C.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2014) Biochemistry, University of Washington, Box
            351655, Room 420, Seattle, WA 98195-1655, USA
COMMENT     ##Assembly-Data-START ##Sequencing Technology :: Sanger dideoxy
            sequencing                ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          join(complement(1..>2646),complement(<2848..9199))
                     /organism="Cloning vector pMU125"
                     /mol_type="other DNA"
                     /db_xref="taxon:1544017"
                     /lab_host="Escherichia coli"
     rep_origin      complement(80..699)
     CDS             complement(854..>1173)
                     /transl_table=11
                     /product="beta-lactamase TEM"
                     /protein_id="AIO09751.1"
                     /db_xref="GI:685453763"
                     /translation="AAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPA
                     GWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLI
                     KHW"
                     /codon_start=3
     misc_feature    1174..2264
                     /standard_name="apramycin cassette"
     CDS             complement(1174..1986)
                     /gene="ApraR"
                     /product="Apramycin resistance gene aac(3) IV"
     CDS             join(2353..>2642,<2844..3468)
                     /note="class C tetracycline resistance protein"
                     /codon_start=1
                     /transl_table=11
                     /product="TetA"
                     /protein_id="AIO09752.1"
                     /db_xref="GI:685453764"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH
                     YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLCRPMPL
                     RAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSL
                     AVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILL
                     ASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSIIGPLIVTAIYAASASTWNG
                     LAWIVGAALYLVCLPALRRGAWSRATST"
     regulatory      2653..2687
                     /standard_name="J23119 promoter"
     misc_feature    2688..2707
                     /standard_name="sgRNA for mCherry"
     misc_RNA        2708..2749
                     /standard_name="dCas9 handle"
     regulatory      2750..2790
                     /standard_name="S.pyogenes terminator"
     CDS             complement(4250..5164)
                     /note="Length: 915"
                     /note="Start codon: TTG"
                     /standard_name="mobA/mobL"
     rep_origin      complement(6245..7581)
     CDS             complement(7942..8904)
                     /note="Length: 963"
                     /note="Start codon: CTG"
                     /standard_name="MobA/MobL"
ORIGIN      
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgctg cagtcatgag ctcagccaat cgactggcga
     1201 gcggcatcgc attcttcgca tcccgccctc tggcggatgc aggaagatca acggatctcg
     1261 gcccagttga cccagggctg tcgccacaat gtcgcgggag cggatcaacc gagcaaaggc
     1321 atgaccgact ggaccttcct tctgaaggct cttctccttg agccacctgt ccgccaaggc
     1381 aaagcgctca cagcagtggt cattctcgag ataatcgacg cgtaccaact tgccatcctg
     1441 aagaatggtg cagtgtctcg gcaccccata gggaaccttt gccatcaact cggcaagatg
     1501 cagcgtcgtg ttggcatcgt gtcccacgcc gaggagaagt acctgcccat cgagttcatg
     1561 gacacgggcg accgggcttg caggcgagtg aggtggcagg ggcaatggat cagagatgat
     1621 ctgctctgcc tgtggccccg ctgccgcaaa ggcaaatgga tgggcgctgc gctttacatt
     1681 tggcaggcgc cagaatgtgt cagagacaac tccaaggtcc ggtgtaacgg gcgacgtggc
     1741 aggatcgaac ggctcgtcgt ccagacctga ccacgagggc atgacgagcg tccctcccgg
     1801 acccagcgca gcacgcaggg cctcgatcag tccaagtggc ccatcttcga ggggccggac
     1861 gctacggaag gagctgtgga ccagcagcac accgccgggg gtaaccccaa ggttgagaag
     1921 ctgaccgatg agctcggctt ttcgccattc gtattgcacg acattgcact ccaccgctga
     1981 tgacatcagt cgatcatagc acgatcaacg gcactgttgc aaatagtcgg tggtgataaa
     2041 cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc cggcattttc
     2101 agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc agttaccgtg
     2161 agccggatca gtgagggttt gcaactgcgg gtcaaggatc tggatttcga tcacggcacg
     2221 atcatcgtgc gggagggcaa gggctccaag gatcgggcct tgatgaattc tcatgtttga
     2281 cagcttatca tcgataagct ttaatgcggt agtttatcac agttaaattg ctaacgcagt
     2341 caggcaccgt gtatgaaatc taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg
     2401 gatgctgtag gcataggctt ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc
     2461 cattccgaca gcatcgccag tcactatggc gtgctgctag cgctatatgc gttgatgcaa
     2521 tttctatgcg cacccgttct cggagcactg tccgaccgct ttggccgccg cccagtcctg
     2581 ctcgcttcgc tacttggagc cactatcgac tacgcgatca tggcgaccac acccgtcctg
     2641 tggatccgat ctttgacagc tagctcagtc ctaggtataa tactagtaac tttcagttta
     2701 gcggtctgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     2761 aaaaagtggc accgagtcgg tgcttttttt gaagcttggg cccgaacaaa aactcatctc
     2821 agaagaggat ctgaatagcg ccgtcgaccg atgcccttga gagccttcaa cccagtcagc
     2881 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     2941 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
     3001 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
     3061 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
     3121 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
     3181 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
     3241 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     3301 gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc
     3361 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc
     3421 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc
     3481 ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa
     3541 ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca
     3601 gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc
     3661 tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat
     3721 caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa
     3781 caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc
     3841 cctgcaccat tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac
     3901 ctacatctgt attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc
     3961 gcatccatac cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat
     4021 cagtaacccg tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca
     4081 gaaatccccc ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg
     4141 cccgctttat cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg
     4201 atgaacaggc agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct
     4261 gctgctgttg ctgctgttca agtgtcaatt gctctactct gttgctcgtc tcaatgatag
     4321 acatatcaag cctttcagct tttttattgc gctttttgat ttcatcatta cgcttgacga
     4381 tctccagttc ctcccccctg cgcttggcgt gggtggcttc aagtccctcc ttgaatgtcg
     4441 gctcgatgtc tagtccacga tccttatgac tgcggtgatc aactctcacc tcaagccctg
     4501 ctcgctctag gtggacgttc gtcagatccg ccactttttc cctgattttt ttcagcgttg
     4561 agttctgatc tagctccctg actttcttcc ctagcccttg cggtgtaagg cttcttgttg
     4621 tcattagtat gtgggcgtga tggtttcttt catcacttcc cccatgcaca tgaggggcat
     4681 ggatcgctac gtccaccgcc actccatagg ctttaactaa ggactggcac aactcggtaa
     4741 caagtgcctg acgctgtgtc ttatccagtt catgcggcaa tgctatctct acttcctttg
     4801 ctaatcttgc ctcctgtttg atgtcaccgt tcttttttag ttcggattgc tctacccgat
     4861 tccatagggt ttgacgatct aacatgtcag gacttgcccc agttggggca aaaatttggg
     4921 tgtattcaat gcctgttttt ttggtgtagt cctgctcttt tccgtacgta tcacagtaca
     4981 atttttcgcc tgctcggtat gctgcacatg ccacgattga gcgaccatct gacctcgaaa
     5041 tgttctgcat ttcacaatgg taaattgcca ttttcatcac ctgtttatta acagccgccc
     5101 caagttttgg gcggttgggg tgtcggggtt tccctgaccg tacttgcaaa atttggcgta
     5161 gcaaaaattt tgcgtaagtg cgcccttcgg gaactctgcg aggctaaaag caaaagcaag
     5221 agcaataagg ctttgacttt gttttttgat tttgctcgct gcgctcggat cttgatgtaa
     5281 gtcgagtttt tgaagtagaa cacttttcac attgatgatg gttcatactt cggaaaatag
     5341 gttgagacac aggcattaaa aatggtcaaa aaaacagcta tggaattggg tcaaatgctc
     5401 gatgaagaaa aggaaaaatt ggaacgtcag gaaaaggcaa gaaaggatct agctgacgct
     5461 gtggtgaaag gtcgagagca aaaacaggca aggtcagacg atgcaaaacg caagatcctg
     5521 atcggggctt acttactgaa gaaacatgat aatgacatca agaaacttgt ggaacaaaac
     5581 cccgatttta ttgggtacat tagagaaaat gataagcact tattttcata ggcattagta
     5641 gttataaaaa accctcataa atcgagggtt tcttttttat ctgcattact ttttgaatca
     5701 atgtactttt ttagcttgct atttttcact gcatacagcc gcttatactc tttctcaatc
     5761 aatgcggtca aaatggcttt ttggctctcc cctgctgcgc ccaccatgtc cgcaagcata
     5821 tccgacacac ccttatctat aaacacctcc aaacgttgac tatcaaggct tttgcgtttt
     5881 tcacgatatg ctttttgtcg ttcggcattg gtttttggcg gctcttttag catgtcctgt
     5941 gttttctgat cgttcgggtc tttcatgtct ttaactccca tatagtatat catagttaca
     6001 ggataacatt gtttttagtt acaggataac tttttataaa atttatttgt aaaaatttat
     6061 gctatcctgc aactattttt aaattacagg ataactattt aacgttaccc tgtaactata
     6121 gttatcctgt aacgctaaat taattaccct gtaacatata ttttgttatc ctgtaacaac
     6181 taaatattga aataatacta ccaaagttac cctgtaacga aaataactag gttaaaaaat
     6241 gatcggattt taacattttg cgttgttcca aaagttatca acagcctaga acgtcatagg
     6301 aagcgattac agacacttta tagctatcag catgggaaca taagggcagg atgaaatatg
     6361 ggtcttaaaa cgcaaatggt gaggttttag aggtattttt tgaagatgat taaggcggtt
     6421 tgtttttaaa atttttggcg gctctcaggc tgcttacatt ttaaccagtt cagtgaaaag
     6481 ttctttttca gcaaatttct gtttagcacc atagctaaaa cttgcgtgga acatattaag
     6541 aattgaccga aatgacacaa tctcaattat attttttttg aaaagttttc tttatcaaat
     6601 attttaaatc attgatttat atataagtat acattcattt taataattaa tctttattta
     6661 acaatgattt atctatattc aattgtttaa ttattcttac taatattatc tctatatcaa
     6721 tattttttat ttaaaaacat atgtttagta gtgcttttga ttaaagtacc agagggaggg
     6781 agcagagctg aatgggaaat actcacccta gagcgattct taaaaatcac cctaaagtat
     6841 tcccattcga tgtaccgtcg gtcggtcgct ttcgcatcag ggatgacatc actgtatcaa
     6901 gctgccactg ttatgattac gattgatagc accgcctgaa cacgctcata accgccaatt
     6961 aaatgactac tcattgcgtc cgctactctg ttcagttccc ctagtaatag cgtttttccg
     7021 atgtgtgcta gcgtcactgt acctcatcac ccacacatgg acagattgag ttacagacat
     7081 tggctaaatt tttggggtct atgcttgaca aagcgagtta aaaccttaga atttaagaca
     7141 ggtacattaa gcccctgtgg tgaaatcatt agcggtcgtc aaaccttaat gctttcgatt
     7201 gccgatatgt cagtatcgaa gatctgtacc ccataaatta cggtaaagcc ccaagcaatt
     7261 gcaaggggct tttatctttt ttaacaaaaa aaatttataa atcaggattt tataacacta
     7321 aataatccaa agtacatgag taagtcatga ccactcctgc gatgtgtgta gcactctgag
     7381 tatccgtatt atatcagtga tgtcatatac aaccacataa tgcggatgta tcactaactc
     7441 cctagtatct ttctgtctgc ctgtgcgccc catcagtcga ttatcaacaa gtaaatctgt
     7501 cttttcttca attaaatcat caatttcaat agctgctata gggttgcgtt cttcaagata
     7561 atcatagata tttctacgat cttggcgagc tgtttcagtc cactcaatct tcattcttcg
     7621 ctgccagttt cgccaatgtt gcagcacgtc tagcggcaaa ctctgccttt acgtcctcat
     7681 gtggaatcaa cttgcctgca ttggcttcat ttaagccgat ctgtacctgc tgcttgaacc
     7741 atttgtcata gtctgcggct tcctgttgct tcttaacaaa gtcacgcatg aagtcacgga
     7801 ttagctgcgc ccccgaccga tcaacagtct tagcaaggct tgaaaattct tgctttaggt
     7861 catggtctac ccgaaaggta aaagtcgctt ctgtcatgtt tttcactcca atgtgttaca
     7921 tttgaatgct attgtaacac attaaaaagt taggtcattg tcttttttcg gcttaggctg
     7981 ttcttggtca taacgtggtt taaatgatgg ctgtcgctca ttctcaagta aaattttccg
     8041 tttattaaca gcctgttgca tctgctcata gtaaggcatc aactgcggta aaatatcagc
     8101 aagtgcctgt ctgtctttat ccaggatacg gctcaattgc ccgatttttt tcaagtcacg
     8161 ctcataggct attctgtcgc tgatctgctc gtatttttgc tttgaacacc ttatgtactc
     8221 ttgcatatcg ttacttgccc atgcctgcgc ctgaagacgt gtaaacatgg aaatgtctga
     8281 agcaacatgg tcgctgatat ggtcatactc agcatctttg acatagccgt attttgataa
     8341 atatctttca tagtcctgcc gtttcttttt gtcatcttcc tgtttttgtt tagtttctct
     8401 ttctatctgc tgttgttttc gttgctgttc ctgttgctcc tcttttatct ctctgtgata
     8461 aatggaaagg ctagattgat cattatccca acgtgtgagc atgtcctcaa agccgtaata
     8521 atcagcacta aaccaacctg ttttcacttt cactggtggc ggcagtggct ctccgatctc
     8581 ccttagcagt gctgtagctg ctcgtccctc tttgatcact ttatcaagct gcaagcggtc
     8641 gatattgtct tgcaaagcct tgtaaaatct atgctctgct cgcttctcat ccgtttttac
     8701 ttcaatcaaa tacacatcta taagcgtttg gcggtgttgt tggtggtcgt atagtgcctg
     8761 ttgtgcggtg gcatagactg cgggggcttg gtctacggca tcctccagct gcctcgcgcg
     8821 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     8881 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     8941 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     9001 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     9061 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     9121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     9181 ttatccacag aatcagggg
//
