LOCUS       MUT_S1-H1        5403 bp DNA     circular SYN 02-MAY-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Mauricio
  TITLE     Direct Submission
  JOURNAL   Exported Jul 15, 2023 from SnapGene Viewer 7.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    19..289
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        322..360
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     exon            375..535
                     /label=Exon 1
                     /note="The 5' end of this exon has not been accurately 
                     mapped. This exon is a hybrid between CMV IE1 exon 1 and 
                     CHO DHFR exon 1."
     primer_bind     392..416
                     /label=oMA179_CMV_Exon1-F
     primer_bind     433..455
                     /label=oMA002_1st_Exon_PCR-F
     primer_bind     460..481
                     /label=oMA051_ExonID_v2-F
     intron          536..834
                     /label=Intron 1
                     /note="This intron is derived from CHD DHFR intron 1. A Not
                     I site has been added to facilitate cloning procedures."
     misc_feature    836..1083
                     /label=SRSF1 binding region (15 modules)
     misc_feature    1086..1094
                     /label=Splice site (Major)
                     /note="Major spliceosome: consensus sequence CAGGTAAGT"
     misc_feature    1095..1486
                     /label=hnRNP A1 binding region (24 modules)
     intron          1487..2115
                     /label=intron 2
                     /note="This intron is derived from CHO DHFR intron 3."
     exon            2116..2888
                     /label=Exon 3
                     /note="This exon is derived from a concatenation of CHO 
                     DHFR exons 4, 5 and 6. The 3' end of this exon has not been
                     mapped accurately."
     primer_bind     complement(2197..2227)
                     /label=oMA203_Common3-R
     primer_bind     complement(2738..2755)
                     /label=oMA003_Long_cDNAGen
     polyA_signal    2740..2874
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2745..2778)
                     /label=oMA180_SV40_PCR-R
     primer_bind     complement(2765..2783)
                     /label=oMA034_Minimal_Seq-R
     primer_bind     complement(2779..2796)
                     /label=oMA178_SV40_cDNAGen
     primer_bind     complement(2814..2850)
                     /label=SBb-PCR-R
     primer_bind     complement(2817..2861)
                     /label=SBb-PCR-R3
     primer_bind     complement(2856..2888)
                     /label=WholeMinigene-R
     primer_bind     complement(2860..2888)
                     /label=oMA204_cDNAGen_End
     primer_bind     complement(3500..3527)
                     /label=oMA078_Fixing_BsaI-less-R3
     primer_bind     3505..3523
                     /label=oMA075_Fixing_BsaI-less-F3
     rep_origin      complement(3584..4172)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4343..5203)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5204..5308)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttcactcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
       61 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
      121 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      181 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      241 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      301 cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct
      361 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
      421 agacaccggg accgatccag cctccgcgta cggttcgacc gctgaactgc atcgtcgccg
      481 tgtcccagaa taagggcatc ggcaagaacg gagaccttcc ctggccaaag ctcaggtact
      541 ggctggattg ggttagggaa accgaggcgg ttcgctgaat cgggtcgagc acttggcgga
      601 gacgcgcggg ccaactactt agggacagtc atgaggggta ggcccgccgg ctgcagccct
      661 tgcccatgcc cgcggtgatc cccatgctgt gccagccttt gcccagaggc gctctagctg
      721 ggagcaaagt ccggtcactg ggcagcacca ccccccggac ttgcatgggt agccgctgag
      781 atggagcctg agcacacgcg gccgctgtta acgcagtgtt tctctttttt tcaggccaaa
      841 caagaggaac gccaaacaag aggaacgcca aacaagagga acgccaaaca agaggaacgc
      901 caaacaagag gaacgccaaa caagaggaac gccaaacaag aggaacgcca aacaagagga
      961 acgccaaaca agaggaacgc caaacaagag gaacgccaaa caagaggaac gccaaacaag
     1021 aggaacgcca aacaagagga acgccaaaca agaggaacgc caaacaagag gaacgccaaa
     1081 caacacaggt aagtccaaac aattagggaa ccaaacaatt agggaaccaa acaattaggg
     1141 aaccaaacaa ttagggaacc aaacaattag ggaaccaaac aattagggaa ccaaacaatt
     1201 agggaaccaa acaattaggg aaccaaacaa ttagggaacc aaacaattag ggaaccaaac
     1261 aattagggaa ccaaacaatt agggaaccaa acaattaggg aaccaaacaa ttagggaacc
     1321 aaacaattag ggaaccaaac aattagggaa ccaaacaatt agggaaccaa acaattaggg
     1381 aaccaaacaa ttagggaacc aaacaattag ggaaccaaac aattagggaa ccaaacaatt
     1441 agggaaccaa acaattaggg aaccaaacaa ttagggaacc aaacaagcta gctggccagt
     1501 gagatccaag aatcttcctg tctctgctga tccactgata ggattacaag tacatgccac
     1561 caagcccagc ttcctcttac catctgctgg ggaccaaact taggccctca ttcctacaca
     1621 gtgaatactt gactttgtta tcacccaacc ctaataaata actcactatc caaacaagtt
     1681 gaaaccctta gaattctgtg ttgctccagc atgatgttgt ggtaaacgtt aatacaataa
     1741 gatgcacagg tcataagtgc acattagcta agtgttgaca aagacttaga cctacataac
     1801 ttaaccctat tagccctcca gaaagttcct cattctccat tccaggcaac tttcatcaca
     1861 ccacatcatg tacaactact attgaagttg ttttccacta tagatacaat gagatgtcac
     1921 atacggcttt gtgttttgat ttgcaagtac caatcgagta tgaaatatgg agtggatatt
     1981 ggacattggc caccatctaa atactttgtg ttaaaagaat tggttttcat aatttgtttt
     2041 gtactgactg ctggctagtc agattacctg actagtatgg acaggatttt gcaataatca
     2101 taattctttt ttcggggaac caccacaagg agctcatttt cttgccaaaa gtctggacga
     2161 agccttaaaa cttattgaac aaccagagtt agcagataaa gtggagctgt catggtttgg
     2221 atagttggag gcagttccgt ttacaaggaa gccatgaatc agccaggcca tctcagactc
     2281 tttgtgacaa ggatcatgca ggaatttgaa agtgacacgt tcttcccaga aattgatttg
     2341 gagaaatata aacttctccc agagtaccca ggggtccttt ctgaagtcca ggaggaaaaa
     2401 ggcatcaagt ataaatttga agtctatgag aagaaaggct aacagaaaga tacttgctga
     2461 ttgacttcaa gttctactgc tttcctccta aaattatgca tttttacaag accatgggac
     2521 ttgtgttggc tttagatcta tgagttattc tttctttaga gagggatagt taggaagatg
     2581 tatttgtttt gtggtaccag agatggaacc tgggatcctg tgcatcctgg gcaactgttg
     2641 tactctaagc cactccccaa agtcatgccc cagcccctgt ataattctaa acaattagaa
     2701 ttattttcat tttcattagt ctaaccaggt tatctagagg atccagacat gataagatac
     2761 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     2821 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     2881 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     2941 aagtaaaacc tctacaaatg tggtatggct gattatgatc ctgcaagcct cgtcgtctgg
     3001 ccggaccacg ctatctgtgc aaggtccccg gacgcgcgct ccatgagcag agcgcccgcc
     3061 gccgaggcaa gactcgggcg gcgccctgcc cgtcccacca ggtcaacagg cggtaaccgg
     3121 cctcttcatc gggaatgcgc gcgaccttca gcattgccgg catgtcccct ggcggacggg
     3181 aagtatcagc tcgaccaagc ttggcgagat tttcaggagc taaggaagct aaaatggaga
     3241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     3301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg cattaatgaa
     3361 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     3421 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     3481 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     3541 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3601 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3661 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3721 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     3781 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3841 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3901 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3961 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4021 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     4081 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4141 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4201 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4261 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4321 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     4381 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     4441 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     4501 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     4561 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     4621 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     4681 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     4741 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     4801 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     4861 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     4921 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     4981 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5041 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5101 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     5161 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     5221 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     5281 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     5341 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     5401 gtc
//