LOCUS       p7INT-Pveg_lacI         5599 bp DNA     circular SYN 20-MAR-2024
DEFINITION  synthetic circular DNA.
ACCESSION   urn.local...19q-gtzbueu
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 20, 2024 from SnapGene Viewer 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     -35_signal      283..288
                     /standard_name="Putative\-35\ermB"
                     /label=Putative -35 ermB
     -10_signal      307..315
                     /standard_name="Putative\-10\ermB"
                     /label=Putative -10 ermB
     RBS             356..362
                     /standard_name="ermB\RBS"
                     /label=ermB RBS
     CDS             371..1105
                     /standard_name="ermB"
                     /label=ermB
     promoter        1190..1309
                     /standard_name="5'\of\Integrase\cloned\from\S\pyogenes"
                     /label=Promoter region
     -35_signal      1257..1262
                     /standard_name="Putative\-35\Integrase"
                     /label=Putative -35 Integrase
     -10_signal      1278..1286
                     /standard_name="Putative\-10\Integrase"
                     /label=Putative -10 Integrase
     CDS             1310..2398
                     /standard_name="int\Integrase"
                     /label=int Integrase
                     /note="Compare with HSC5 T12 Integrase: Identities =
                     1078/1089 (98%), Gaps = 0/1089 (0%)"
     misc_feature    2402..2574
                     /standard_name="attP"
                     /label=attP
     misc_feature    2402..2440
                     /standard_name="P"
                     /label=P
     misc_feature    2441..2536
                     /standard_name="0"
                     /label=0
     misc_feature    2537..2574
                     /standard_name="P'"
                     /label=P'
     misc_feature    2575..2749
                     /standard_name="Homologous\to\HSC5"
                     /label=Homologous to HSC5
     CDS             complement(2611..2736)
                     /standard_name="CDS_61\in\HSC5"
                     /label=CDS_61 in HSC5
     CDS             complement(3047..3235)
                     /standard_name="lacZ"
                     /label=lacZ
     primer_bind     3192..3208
                     /label=M13_f
     promoter        3241..3407
                     /standard_name="Pveg"
                     /label=Pveg promoter
     promoter        3327..3332
                     /standard_name="-35"
                     /label=-35 promoter
     promoter        3348..3356
                     /standard_name="-10"
                     /label=-10 promoter
     misc_feature    3408..3414
                     /standard_name="RBS"
                     /label=nonstandard type: Ribosome Binding site
     CDS             3423..4505
                     /codon_start=1
                     /standard_name="lacI"
                     /label=lacI
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     terminator      4518..4547
                     /standard_name="tt3"
                     /label=tt3 terminator
     gene            complement(4548..4597)
                     /standard_name="lacZ"
                     /label=lacZ
     primer_bind     complement(4598..4614)
                     /label=M13_r
     promoter        complement(4603..4724)
                     /standard_name="lac\promoter"
                     /label=lac promoter
     -10_signal      complement(4647..4652)
                     /standard_name="-10\lacZ"
                     /label=-10 lacZ
     -35_signal      complement(4671..4676)
                     /standard_name="-35\lacZ"
                     /label=-35 lacZ
     rep_origin      4985..5599
ORIGIN
        1 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
       61 catgagatta tcaaaaagga tcttcaccta gatccttttg atcccccaca aaaagaaaaa
      121 cgaaatgata caccaatcag tgcaaaaaaa gatataatgg gagataagac ggttcgtgtt
      181 cgtgctgact tgcaccatat cataaaaatc gaaacagcaa agaatggcgg aaacgtaaaa
      241 gaagttatgg aaataagact tagaagcaaa cttaagagtg tgttgatagt gcagtatctt
      301 aaaattttgt ataataggaa ttgaagttaa attagatgct aaaaatttgt aattaagaag
      361 gagtgattac atgaacaaaa atataaaata ttctcaaaac tttttaacga gtgaaaaagt
      421 actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt acgaaattgg
      481 aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg taacgtctat
      541 tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt
      601 cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga ggtataaaat
      661 tgttgggagt attccttacc atttaagcac acaaattatt aaaaaagtgg tttttgaaag
      721 ccatgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta ccttggatat
      781 tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat tgcttaagct
      841 gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa aacttacccg
      901 ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg tttcaaaatg
      961 ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag caatgaaaca
     1021 cgccaaagta aacaatttaa gtaccgttac ttatgagcaa gtattgtcta tttttaatag
     1081 ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttgtaaa tttggaaagt
     1141 tacacgttac taaagggaat gtagataaat tattaggtaa atctaaaaca aaaagcccca
     1201 cgctctcaaa gtttggcgac tctgagcgtg aggcaagaca gtataagaaa caaccattaa
     1261 aaaggtcatt ttcttgtacc tattttatca aattgaaaga tggtatgcaa tgaaaattaa
     1321 atcatataaa aaggaaaatg gtgaaactgc ttataaattt cttttgtatg ccggttatgt
     1381 taatggaaag agaaaatata ttaggcgaga aggtttcaaa actaagcagg ctgcaaggga
     1441 aaccttaatt agtttacaag ctgaacttga taaacctaaa tcaagtatga catttggagc
     1501 attgacagat caatggctaa aggaatatga aaaaaccgtt cagggcagta cctacttaaa
     1561 aacagaaaga aatattaata aacatatttt gccaaaactt gataaagtga agattggaga
     1621 catcaatcca ctacttatcc agcggcttac tgaagaatgg tgcaacgatt taaaatatgg
     1681 aggaaaaatt cttgggcttg ttaggaatat cttaaatcta gctgttagat acggatatat
     1741 caataacaat ccagctttgc caattacacc tccaaaaata aaaaggaaaa gaaaaatgaa
     1801 taataatttt tatacacttg atcaacttaa acaattcctt gaactagttg aaaaaactga
     1861 caacattgaa aaaatagcct tgtttagatt attagcattt actggaatac gaaaagggga
     1921 gcttctggca ctaacttggg atgatttgaa tggtaatact ctatcaatta ataaagctgt
     1981 cacacgtact caagttggac tagaaataga tgttacgaag acaaaatcaa gcgatagatt
     2041 aatcagctta gatgatgaaa ctttggaaat tttacaagaa cttcatgaaa cttttcctac
     2101 ttctactctt atgttccaat ctaaatcagg tggaattatg acgccaagtt taccacgaaa
     2161 atggctattg caaattatca aagggataga cttaccacaa atcacaattc atggtttcag
     2221 gcacactcat gcaagcttac ttttcgaatc aggtctatcc ttgaaacagg tgcaacatag
     2281 attagggcat ggagatttac agacaactat gaacgtatat actcacatca cgcaatcggc
     2341 aattgatgac attggaacta aattcaatca atttgttact aacaagcaac tagattgaca
     2401 actaattctc aacaaacgtt aatttaacaa cattcaagta actcccacca gctccatcaa
     2461 tgcttaccgt aagtaatcat aacttactaa aaccttgtta catcaaggtt ttttcttttt
     2521 gtcttgttca tgagttacca taactttcta tattattgac aactaaattg acaactcttc
     2581 aattattttt ctgtctactc aaagttttct tcatttgata tagtctaatt ccaccatcac
     2641 ttcttccacc ttctcctctg tcacaacttc tccatttctc gcttcctcat gcggcaacac
     2701 ataatcaaaa atctgtccgt ttttacgcac gatcataact gtgtctccta aaatataccc
     2761 cttatcaatc gcttctttaa actcatctat atataacata tttcatcctc ctacctatct
     2821 attcgtaaaa agataaaaat aactattgtt ttgtttgtta ttttataata aaattattaa
     2881 tataagttaa tgttttttaa aaatatacaa ttttattcta tttatagtta gctatttttt
     2941 cattgttagt aatattggtg aattgtaata acctttttta ctactgacag cttccaagga
     3001 gctaaagagg tccctagggg taccgagctc gaattattaa cgcttacaat ttccattcgc
     3061 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     3121 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     3181 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta taggggcggg
     3241 ttttttattt tacataatga tacataattt accgaaactt gcggaacata attgaggaat
     3301 catagaattt tgtcaaaata attttattga caacgtctta ttaacgttga tataatttaa
     3361 attttatttg acaaaaatgg gctcgtgttg tacaataaat gtagttaaag gaggaataaa
     3421 aaatgaaacc agtcaccctt tacgatgtcg cagaatacgc aggagtctct taccaaacag
     3481 tcagtcgtgt agtcaatcag gctagtcatg tatcagcaaa aactcgtgaa aaggttgaag
     3541 ctgcaatggc tgaacttaat tacatcccaa atcgtgttgc ccaacagttg gctggtaaac
     3601 aaagtttgct tatcggagtt gctacctctt cattggcact tcatgcccct tctcaaattg
     3661 ttgccgctat caaatctcgt gcagatcaat tgggtgcaag tgttgtcgta tctatggttg
     3721 aacgtagtgg agtcgaagcc tgtaaggcag ccgtccacaa tttattggct caacgtgtat
     3781 ctggtttaat tatcaattac ccattggatg accaagatgc cattgctgtc gaagctgcat
     3841 gtaccaatgt accagcatta tttttggatg tttcagatca aacacctatc aattcaatca
     3901 tctttagtca tgaagatggt actcgtttgg gagtcgaaca tttggtagct cttggtcacc
     3961 aacagattgc tcttttagca ggaccactta gttctgtttc agcccgtctt cgtttagctg
     4021 gttggcacaa atacttaact cgtaatcaaa ttcagcctat cgctgaacgt gagggtgatt
     4081 ggtctgcaat gtcaggattt caacagacaa tgcaaatgtt gaatgaaggt attgtaccaa
     4141 ctgcaatgtt ggttgccaat gaccaaatgg cccttggagc tatgcgtgca attaccgaat
     4201 caggtttgcg tgttggagct gatattagtg ttgtcggtta cgatgataca gaagattcaa
     4261 gttgttacat tccacctttg acaactatca agcaagattt tcgtttgctt ggacagacaa
     4321 gtgttgatcg tttattgcaa ctttctcagg gtcaggctgt caagggaaat cagcttttac
     4381 ctgtatctct tgttaagcgt aagaccacat tagcaccaaa tactcaaacc gcctcacctc
     4441 gtgccttggc agatagtctt atgcagcttg cacgtcaggt ctctcgtttg gaaagtggac
     4501 aataagatcg atcgatcaaa aaaggcccac aaaagtgggc cttttttcat agcttgagta
     4561 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     4621 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     4681 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     4741 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     4801 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     4861 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     4921 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4981 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     5041 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     5101 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     5161 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     5221 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     5281 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     5341 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     5401 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     5461 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     5521 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     5581 gatctcaaga agatccttt
//
