LOCUS       pSpy1C-tt0-PgyrA        5773 bp DNA     circular SYN 23-APR-2024
DEFINITION  Golden Gate ligation product.
ACCESSION   urn.local...194-gltlsu6
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 23, 2024 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4..20)
                     /label=M13_r
     -35_signal      481..486
                     /label=-35
     -10_signal      504..509
                     /label=-10
     CDS             573..1220
                     /codon_start=1
                     /label=Cm(R)
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     CDS             complement(1598..2293)
                     /codon_start=1
                     /label=REP A
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     CDS             complement(2362..2520)
                     /codon_start=1
                     /label=REP C
                     /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA
                     RKESEQKK"
     terminator      3669..3703
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     3739..3756
                     /label=M13 fwd
     promoter        3779..3978
                     /label=PgyrA
                     /note="/created_by=lautenschlaeger"
     5'UTR           4016..4106
                     /label=5'UTR
                     /note="/created_by=lautenschlaeger"
     misc_feature    4016
                     /label=+1
     gene            4107..5759
                     /label=firefly luciferase
                     /note="/created_by=lautenschlaeger"
ORIGIN
        1 tgtgtcatag ctgtttcctg caaagaatgg tgatgacaat tgtacaggtt atcaataaag
       61 ccattgatac tctcattctt atcccgtatt tattaacttc tcctagtatt cgtaattatc
      121 atggctgtca tggcgcatta acggaataaa gggtgtgctt aaatcgggcc attttgcgta
      181 ataagaaaaa ggattaatta tgagcgaatt gaattaataa taaggtaata gatttacatt
      241 agaaaatgaa aggggatttt atgcgtgaga atgttacagt ctatcccggc aatagttacc
      301 cttattatca agataagaaa gaaaaggatt tttcgctacg ctcaaatcct ttaaaaaaac
      361 acaaaagacc acatttttta atgtggtctt tattcttcaa ctaaagcacc cattagttca
      421 acaaacgaaa attggataaa gtgggatatt tttaaaatat atatttatgt tacagtaata
      481 ttgactttta aaaaaggatt gattctaatg aagaaagcag acaagtaagc ctcctaaatt
      541 cactttagat aaaaatttag gaggcatatc aaatgaactt taataaaatt gatttagaca
      601 attggaagag aaaagagata tttaatcatt atttgaacca acaaacgact tttagtataa
      661 ccacagaaat tgatattagt gttttatacc gaaacataaa acaagaagga tataaatttt
      721 accctgcatt tattttctta gtgacaaggg tgataaactc aaatacagct tttagaactg
      781 gttacaatag cgacggagag ttaggttatt gggataagtt agagccactt tatacaattt
      841 ttgatggtgt atctaaaaca ttctctggta tttggactcc tgtaaagaat gacttcaaag
      901 agttttatga tttatacctt tctgatgtag agaaatataa tggttcgggg aaattgtttc
      961 ccaaaacacc tatacctgaa aatgcttttt ctctttctat tattccatgg acttcattta
     1021 ctgggtttaa cttaaatatc aataataata gtaattacct tctacccatt attacagcag
     1081 gaaaattcat taataaaggt aattcaatat atttaccgct atctttacag gtacatcatt
     1141 ctgtttgtga tggttatcat gcaggattgt ttatgaactc tattcaggaa ttgtcagata
     1201 ggcctaatga ctggctttta taatatgaga taatgccgac tgtacttttt acagtcggtt
     1261 ttctaatgtc actaacctgc cccgttagtt gaagaaggtt tttatattac agctccagat
     1321 ccatatcctt ctttttctga accgacttct cctttttcgc ttctttattc caattgcttt
     1381 attgacgttg agcctcggaa cccttaacaa tcccaaaact tgtcgacttt cgtcaggggg
     1441 gcttttattt attcaataat ccctcctctc aataaatcta ttgttgtact taattcaact
     1501 tccatttctc tgtatctttc aatacgctct tttaagtcct taatttcttt ttttaattcc
     1561 tcattttcag caaataactc tttttctttg tttgtcattt tatttccccc gtttcagcat
     1621 caagaacctt tgcataactt gctctatatc cacactgata attgccctca aaccataatc
     1681 taaaggcgct agagtttgtt gaaacaatat cttttacatc attcgtattt aaaattccaa
     1741 actccgctcc cctaaggcga ataaaagcca ttaaatcttt tgtatttacc aaattatagt
     1801 catccactat atctaaaagt aaattcttca attctctttt ttggctttca tcaagtgtta
     1861 tatagcggtc aatatcaaaa tcattaatgt tcaaaatatc ttttttgtcg tatatatgtt
     1921 tattcttagc aatagcgtcc tttgattcat gagtcaaata ttcatatgaa cctttgatat
     1981 aatcaagtat ctcaacatga gcaactgaac tattccccaa ttttcgctta atcttgttcc
     2041 taacgctttc tattgttaca ggatttcgtg caatatatat aacgtgatag tgtggttttt
     2101 tatagtgctt tccatttcgt ataacatcac tactattcca tgtatcttta tctttttttt
     2161 cgtccatatc gtgtaaagga ctgacagcca tagatacgcc caaactctct aatttttctt
     2221 tccaatcatt aggaattgag tcaggatata ataaaaatcc aaaatttcta gctttagtat
     2281 ttttaatagc catgatatat taccttatca aaaacaagta gcgaaaactc gtatccttct
     2341 aaaaacgcga gctttcgctt attttttttg ttctgattcc tttcttgcat attcttctat
     2401 agctaacgcc gcaaccgcag attttgaaaa acctttttgt ttcgccatat ctgttaattt
     2461 tttatcttgc tcttttgtca gagaaatcat aactcttttt ttcgattctg aaatcaccat
     2521 ttaaaaaact ccaatcaaat aattttataa aattagtgta tcactttgta atcataaaaa
     2581 caacaataaa gctacttaaa tatagattta taaaaaacgt tggcgaaaac gttggcgatt
     2641 cgttggcgat tgaaaaaccc ctcaaaccct tgagccagtt gggatagagc gtttttggca
     2701 caaaaattgg cactcggcac ttaatggggg gtcgtagtac ggaagcaaaa ttcgcttcct
     2761 ttccccccat ttttttccaa attccaaatt tttttcaaaa attttccagc gctaccgctc
     2821 ggcaaaattg caagcaattt ttaaaatcaa acccatgagg gaatttcatt ccctcaaact
     2881 cccttgagcc tcctccaacc gaaatagaag gacgctgcgc ttattatttc attcagtcat
     2941 cggctttcat aatctaacag acaacatctt cgctgcaaag cacgctacgc tcaagggctt
     3001 ttacgctacg ataacgcctg ttttaacgat tatgccgata actaaacgaa ataaacgcta
     3061 aaacgtctca gaaacgattt tgagacgttt taataaaaaa tcgaccgaaa aataaatata
     3121 aatcggatat acaatcggca attgacgaaa ctgcaaaata tcctgtaaag gatacggatt
     3181 ttatgaccga tgatgaagaa aagaatttga aacgtttgtc tgatttggag gaaggtttac
     3241 accgtaaaag gttaatctcc tatggtggtt tgttaaaaga aatacataaa aaattaaacc
     3301 ttgatgacac agaagaaggc gatttgattc atacagatga tgacgaaaaa gccgatgaag
     3361 atggattttc tattattgca atgtggaatt gggaacggaa aaattatttt attaaagagt
     3421 agttcaacaa acgggccagt ttgttgaaga ttagatgcta taattgttat taaaaggatt
     3481 gaaggatgct taggaagacg agttattaat agctgaataa gaacggtgct ctccaaatat
     3541 tcttatttag aaaacgaaat ctaaaattat ctgaaaaggg aatgagaata gtgaatggac
     3601 caataataat gactagagaa gaaagaatga agattgttca tgaaattaag gaacgaatat
     3661 tggataacat aaaaaacgcc cggcggcaac cgagcgttct gaaatatggg gatgatgtta
     3721 aggctattgg tgtttatggt aaaacgacgg ccagtcagaa ttcaaaaaaa aatctagatt
     3781 tttgtgttgt ttagttgcag tcatttctaa acatctcctt aattttatta gagcaaaagc
     3841 tcatacggtc ttattttaac atattgataa ttgtttgtca ttgaattcag aaaaataatg
     3901 aaaatagttt actgataacg cttccaataa gaaaaaataa ggaatttatg gtataatgaa
     3961 atctagtatt gactagaata ctagtactgc agtgaaattc aaaaaaaaat ctagaagaat
     4021 taaattaaaa agggaggcca aatataatga aaaatatgaa tgacaatgat gttccgcggt
     4081 ggcggcgatc taaggaggaa taaaaaatgg aagacgccaa aaacataaag aaaggcccgg
     4141 cgccattcta tcctctagag gatggaaccg ctggagagca actgcataag gctatgaaga
     4201 gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag gtgaacatca
     4261 cgtacgcgga atacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc
     4321 tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg
     4381 tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac
     4441 gtgaattgct caacagtatg aacatttcgc agcctaccgt agtgtttgtt tccaaaaagg
     4501 ggttgcaaaa aattttgaac gtgcaaaaaa aattaccaat aatccagaaa attattatca
     4561 tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc
     4621 tacctcccgg ttttaatgaa tacgattttg taccagagtc ctttgatcgt gacaaaacaa
     4681 ttgcactgat aatgaattcc tctggatcta ctgggttacc taagggtgtg gcccttccgc
     4741 atagaactgc ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca
     4801 ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta
     4861 cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc
     4921 tgtttttacg atcccttcag gattacaaaa ttcaaagtgc gttgctagta ccaaccctat
     4981 tttcattctt cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa
     5041 ttgcttctgg gggcgcacct ctttcgaaag aagtcgggga agcggttgca aaacgcttcc
     5101 atcttccagg gatacgacaa ggatatgggc tcactgagac tacatcagct attctgatta
     5161 cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga
     5221 aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcagagaggc gaattatgtg
     5281 tcagaggacc tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga
     5341 ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact
     5401 tcttcatagt tgaccgcttg aagtctttaa ttaaatacaa aggatatcag gtggcccccg
     5461 ctgaattgga atcgatattg ttacaacacc ccaacatctt cgacgcgggc gtggcaggtc
     5521 ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga
     5581 cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt
     5641 tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg
     5701 caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagtcc aaattgtaat
     5761 actagtactg cag
//
