LOCUS       pSpy1C-PxylS2_ff        5706 bp DNA     circular SYN 23-APR-2024
DEFINITION  Restriction Cloning ligation product.
ACCESSION   urn.local...198-gltlsu6
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 23, 2024 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3669..3703
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
                     /organism="synthetic DNA construct"
     source          3939..4029
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
                     /organism="synthetic DNA construct"
     primer_bind     complement(5..21)
                     /label=M13_r
     -35_signal      482..487
                     /label=-35
     -10_signal      505..510
                     /label=-10
     CDS             574..1221
                     /codon_start=1
                     /label=Cm(R)
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     CDS             complement(1599..2294)
                     /codon_start=1
                     /label=REP A
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     CDS             complement(2363..2521)
                     /codon_start=1
                     /label=REP C
                     /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA
                     RKESEQKK"
     terminator      3669..3703
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     3739..3756
                     /label=M13 fwd
     promoter        3776..3938
                     /label=PxylS2 promoter
                     /note="/created_by=lautenschlaeger"
     5'UTR           3939..4029
                     /label=5'UTR
                     /note="/created_by=lautenschlaeger"
     misc_feature    3939
                     /label=+1
     gene            4030..5682
                     /label=firefly luciferase
                     /note="/created_by=lautenschlaeger"
ORIGIN
        1 atgtgtcata gctgtttcct gcaaagaatg gtgatgacaa ttgtacaggt tatcaataaa
       61 gccattgata ctctcattct tatcccgtat ttattaactt ctcctagtat tcgtaattat
      121 catggctgtc atggcgcatt aacggaataa agggtgtgct taaatcgggc cattttgcgt
      181 aataagaaaa aggattaatt atgagcgaat tgaattaata ataaggtaat agatttacat
      241 tagaaaatga aaggggattt tatgcgtgag aatgttacag tctatcccgg caatagttac
      301 ccttattatc aagataagaa agaaaaggat ttttcgctac gctcaaatcc tttaaaaaaa
      361 cacaaaagac cacatttttt aatgtggtct ttattcttca actaaagcac ccattagttc
      421 aacaaacgaa aattggataa agtgggatat ttttaaaata tatatttatg ttacagtaat
      481 attgactttt aaaaaaggat tgattctaat gaagaaagca gacaagtaag cctcctaaat
      541 tcactttaga taaaaattta ggaggcatat caaatgaact ttaataaaat tgatttagac
      601 aattggaaga gaaaagagat atttaatcat tatttgaacc aacaaacgac ttttagtata
      661 accacagaaa ttgatattag tgttttatac cgaaacataa aacaagaagg atataaattt
      721 taccctgcat ttattttctt agtgacaagg gtgataaact caaatacagc ttttagaact
      781 ggttacaata gcgacggaga gttaggttat tgggataagt tagagccact ttatacaatt
      841 tttgatggtg tatctaaaac attctctggt atttggactc ctgtaaagaa tgacttcaaa
      901 gagttttatg atttatacct ttctgatgta gagaaatata atggttcggg gaaattgttt
      961 cccaaaacac ctatacctga aaatgctttt tctctttcta ttattccatg gacttcattt
     1021 actgggttta acttaaatat caataataat agtaattacc ttctacccat tattacagca
     1081 ggaaaattca ttaataaagg taattcaata tatttaccgc tatctttaca ggtacatcat
     1141 tctgtttgtg atggttatca tgcaggattg tttatgaact ctattcagga attgtcagat
     1201 aggcctaatg actggctttt ataatatgag ataatgccga ctgtactttt tacagtcggt
     1261 tttctaatgt cactaacctg ccccgttagt tgaagaaggt ttttatatta cagctccaga
     1321 tccatatcct tctttttctg aaccgacttc tcctttttcg cttctttatt ccaattgctt
     1381 tattgacgtt gagcctcgga acccttaaca atcccaaaac ttgtcgactt tcgtcagggg
     1441 ggcttttatt tattcaataa tccctcctct caataaatct attgttgtac ttaattcaac
     1501 ttccatttct ctgtatcttt caatacgctc ttttaagtcc ttaatttctt tttttaattc
     1561 ctcattttca gcaaataact ctttttcttt gtttgtcatt ttatttcccc cgtttcagca
     1621 tcaagaacct ttgcataact tgctctatat ccacactgat aattgccctc aaaccataat
     1681 ctaaaggcgc tagagtttgt tgaaacaata tcttttacat cattcgtatt taaaattcca
     1741 aactccgctc ccctaaggcg aataaaagcc attaaatctt ttgtatttac caaattatag
     1801 tcatccacta tatctaaaag taaattcttc aattctcttt tttggctttc atcaagtgtt
     1861 atatagcggt caatatcaaa atcattaatg ttcaaaatat cttttttgtc gtatatatgt
     1921 ttattcttag caatagcgtc ctttgattca tgagtcaaat attcatatga acctttgata
     1981 taatcaagta tctcaacatg agcaactgaa ctattcccca attttcgctt aatcttgttc
     2041 ctaacgcttt ctattgttac aggatttcgt gcaatatata taacgtgata gtgtggtttt
     2101 ttatagtgct ttccatttcg tataacatca ctactattcc atgtatcttt atcttttttt
     2161 tcgtccatat cgtgtaaagg actgacagcc atagatacgc ccaaactctc taatttttct
     2221 ttccaatcat taggaattga gtcaggatat aataaaaatc caaaatttct agctttagta
     2281 tttttaatag ccatgatata ttaccttatc aaaaacaagt agcgaaaact cgtatccttc
     2341 taaaaacgcg agctttcgct tatttttttt gttctgattc ctttcttgca tattcttcta
     2401 tagctaacgc cgcaaccgca gattttgaaa aacctttttg tttcgccata tctgttaatt
     2461 ttttatcttg ctcttttgtc agagaaatca taactctttt tttcgattct gaaatcacca
     2521 tttaaaaaac tccaatcaaa taattttata aaattagtgt atcactttgt aatcataaaa
     2581 acaacaataa agctacttaa atatagattt ataaaaaacg ttggcgaaaa cgttggcgat
     2641 tcgttggcga ttgaaaaacc cctcaaaccc ttgagccagt tgggatagag cgtttttggc
     2701 acaaaaattg gcactcggca cttaatgggg ggtcgtagta cggaagcaaa attcgcttcc
     2761 tttcccccca tttttttcca aattccaaat ttttttcaaa aattttccag cgctaccgct
     2821 cggcaaaatt gcaagcaatt tttaaaatca aacccatgag ggaatttcat tccctcaaac
     2881 tcccttgagc ctcctccaac cgaaatagaa ggacgctgcg cttattattt cattcagtca
     2941 tcggctttca taatctaaca gacaacatct tcgctgcaaa gcacgctacg ctcaagggct
     3001 tttacgctac gataacgcct gttttaacga ttatgccgat aactaaacga aataaacgct
     3061 aaaacgtctc agaaacgatt ttgagacgtt ttaataaaaa atcgaccgaa aaataaatat
     3121 aaatcggata tacaatcggc aattgacgaa actgcaaaat atcctgtaaa ggatacggat
     3181 tttatgaccg atgatgaaga aaagaatttg aaacgtttgt ctgatttgga ggaaggttta
     3241 caccgtaaaa ggttaatctc ctatggtggt ttgttaaaag aaatacataa aaaattaaac
     3301 cttgatgaca cagaagaagg cgatttgatt catacagatg atgacgaaaa agccgatgaa
     3361 gatggatttt ctattattgc aatgtggaat tgggaacgga aaaattattt tattaaagag
     3421 tagttcaaca aacgggccag tttgttgaag attagatgct ataattgtta ttaaaaggat
     3481 tgaaggatgc ttaggaagac gagttattaa tagctgaata agaacggtgc tctccaaata
     3541 ttcttattta gaaaacgaaa tctaaaatta tctgaaaagg gaatgagaat agtgaatgga
     3601 ccaataataa tgactagaga agaaagaatg aagattgttc atgaaattaa ggaacgaata
     3661 ttggataaat aaaaaacgcc cggcggcaac cgagcgttct gaaatatggg gatgatgtta
     3721 aggctattgg tgtttatggt aaaacgacgg ccagtcagcg agaccagctg aattcaatta
     3781 gatatttaaa agtatcatat ctaatattat aactaaattt tctaaaaaaa acattgaaat
     3841 aaacatttat tttgtatatg atgagataaa gttagtttat tggataaaca aactaactca
     3901 attaagatag ttgatggata aacttgttca cttaaatcag aattaaatta aaaagggagg
     3961 ccaaatataa tgaaaaatat gaatgacaat gatgttccgc ggtggcggcg atctaaggag
     4021 gaataaaaaa tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatcctcta
     4081 gaggatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct
     4141 ggaacaattg cttttacaga tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc
     4201 gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga
     4261 atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt
     4321 atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt
     4381 atgaacattt cgcagcctac cgtagtgttt gtttccaaaa aggggttgca aaaaattttg
     4441 aacgtgcaaa aaaaattacc aataatccag aaaattatta tcatggattc taaaacggat
     4501 taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat
     4561 gaatacgatt ttgtaccaga gtcctttgat cgtgacaaaa caattgcact gataatgaat
     4621 tcctctggat ctactgggtt acctaagggt gtggcccttc cgcatagaac tgcctgcgtc
     4681 agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt
     4741 ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata
     4801 tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgttttt acgatccctt
     4861 caggattaca aaattcaaag tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa
     4921 agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc tgggggcgca
     4981 cctctttcga aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc agggatacga
     5041 caaggatatg ggctcactga gactacatca gctattctga ttacacccga gggggatgat
     5101 aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat
     5161 accgggaaaa cgctgggcgt taatcagaga ggcgaattat gtgtcagagg acctatgatt
     5221 atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg
     5281 ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat agttgaccgc
     5341 ttgaagtctt taattaaata caaaggatat caggtggccc ccgctgaatt ggaatcgata
     5401 ttgttacaac accccaacat cttcgacgcg ggcgtggcag gtcttcccga cgatgacgcc
     5461 ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag
     5521 atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg
     5581 tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag
     5641 atcctcataa aggccaagaa gggcggaaag tccaaattgt aatactagta ctgcagtgaa
     5701 ggtctc
//
