LOCUS       pSpy1C-tt0-Plac(        5585 bp DNA     circular SYN 26-MAR-2024
DEFINITION  Golden Gate ligation product.
ACCESSION   urn.local...15h-gtzbudq
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 26, 2024 from SnapGene Viewer 7.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3669..3703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3762..3906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4..20)
                     /label=M13_r
     -35_signal      481..486
                     /standard_name="-35"
                     /label=-35
     -10_signal      504..509
                     /standard_name="-10"
                     /label=-10
     CDS             573..1220
                     /standard_name="Cm(R)"
                     /label=Cm(R)
     CDS             complement(1598..2293)
                     /standard_name="REP A"
                     /label=REP A
     CDS             complement(2362..2520)
                     /standard_name="REP C"
                     /label=REP C
     terminator      complement(3669..3703)
                     /standard_name="lambda t0 terminator"
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     3739..3756
                     /label=M13_f
     promoter        3762..3918
                     /label=Plac(1) promoter
     -35_signal      3819..3824
     -10_signal      3842..3847
     protein_bind    3852..3871
                     /standard_name="lac operator (symmetric)"
                     /label=lac operator (symmetric) binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     RBS             3907..3915
                     /standard_name="RBS mut1"
     gene            3919..5571
                     /standard_name="firefly luciferase"
                     /label=firefly luciferase
ORIGIN
        1 tgtgtcatag ctgtttcctg caaagaatgg tgatgacaat tgtacaggtt atcaataaag
       61 ccattgatac tctcattctt atcccgtatt tattaacttc tcctagtatt cgtaattatc
      121 atggctgtca tggcgcatta acggaataaa gggtgtgctt aaatcgggcc attttgcgta
      181 ataagaaaaa ggattaatta tgagcgaatt gaattaataa taaggtaata gatttacatt
      241 agaaaatgaa aggggatttt atgcgtgaga atgttacagt ctatcccggc aatagttacc
      301 cttattatca agataagaaa gaaaaggatt tttcgctacg ctcaaatcct ttaaaaaaac
      361 acaaaagacc acatttttta atgtggtctt tattcttcaa ctaaagcacc cattagttca
      421 acaaacgaaa attggataaa gtgggatatt tttaaaatat atatttatgt tacagtaata
      481 ttgactttta aaaaaggatt gattctaatg aagaaagcag acaagtaagc ctcctaaatt
      541 cactttagat aaaaatttag gaggcatatc aaatgaactt taataaaatt gatttagaca
      601 attggaagag aaaagagata tttaatcatt atttgaacca acaaacgact tttagtataa
      661 ccacagaaat tgatattagt gttttatacc gaaacataaa acaagaagga tataaatttt
      721 accctgcatt tattttctta gtgacaaggg tgataaactc aaatacagct tttagaactg
      781 gttacaatag cgacggagag ttaggttatt gggataagtt agagccactt tatacaattt
      841 ttgatggtgt atctaaaaca ttctctggta tttggactcc tgtaaagaat gacttcaaag
      901 agttttatga tttatacctt tctgatgtag agaaatataa tggttcgggg aaattgtttc
      961 ccaaaacacc tatacctgaa aatgcttttt ctctttctat tattccatgg acttcattta
     1021 ctgggtttaa cttaaatatc aataataata gtaattacct tctacccatt attacagcag
     1081 gaaaattcat taataaaggt aattcaatat atttaccgct atctttacag gtacatcatt
     1141 ctgtttgtga tggttatcat gcaggattgt ttatgaactc tattcaggaa ttgtcagata
     1201 ggcctaatga ctggctttta taatatgaga taatgccgac tgtacttttt acagtcggtt
     1261 ttctaatgtc actaacctgc cccgttagtt gaagaaggtt tttatattac agctccagat
     1321 ccatatcctt ctttttctga accgacttct cctttttcgc ttctttattc caattgcttt
     1381 attgacgttg agcctcggaa cccttaacaa tcccaaaact tgtcgacttt cgtcaggggg
     1441 gcttttattt attcaataat ccctcctctc aataaatcta ttgttgtact taattcaact
     1501 tccatttctc tgtatctttc aatacgctct tttaagtcct taatttcttt ttttaattcc
     1561 tcattttcag caaataactc tttttctttg tttgtcattt tatttccccc gtttcagcat
     1621 caagaacctt tgcataactt gctctatatc cacactgata attgccctca aaccataatc
     1681 taaaggcgct agagtttgtt gaaacaatat cttttacatc attcgtattt aaaattccaa
     1741 actccgctcc cctaaggcga ataaaagcca ttaaatcttt tgtatttacc aaattatagt
     1801 catccactat atctaaaagt aaattcttca attctctttt ttggctttca tcaagtgtta
     1861 tatagcggtc aatatcaaaa tcattaatgt tcaaaatatc ttttttgtcg tatatatgtt
     1921 tattcttagc aatagcgtcc tttgattcat gagtcaaata ttcatatgaa cctttgatat
     1981 aatcaagtat ctcaacatga gcaactgaac tattccccaa ttttcgctta atcttgttcc
     2041 taacgctttc tattgttaca ggatttcgtg caatatatat aacgtgatag tgtggttttt
     2101 tatagtgctt tccatttcgt ataacatcac tactattcca tgtatcttta tctttttttt
     2161 cgtccatatc gtgtaaagga ctgacagcca tagatacgcc caaactctct aatttttctt
     2221 tccaatcatt aggaattgag tcaggatata ataaaaatcc aaaatttcta gctttagtat
     2281 ttttaatagc catgatatat taccttatca aaaacaagta gcgaaaactc gtatccttct
     2341 aaaaacgcga gctttcgctt attttttttg ttctgattcc tttcttgcat attcttctat
     2401 agctaacgcc gcaaccgcag attttgaaaa acctttttgt ttcgccatat ctgttaattt
     2461 tttatcttgc tcttttgtca gagaaatcat aactcttttt ttcgattctg aaatcaccat
     2521 ttaaaaaact ccaatcaaat aattttataa aattagtgta tcactttgta atcataaaaa
     2581 caacaataaa gctacttaaa tatagattta taaaaaacgt tggcgaaaac gttggcgatt
     2641 cgttggcgat tgaaaaaccc ctcaaaccct tgagccagtt gggatagagc gtttttggca
     2701 caaaaattgg cactcggcac ttaatggggg gtcgtagtac ggaagcaaaa ttcgcttcct
     2761 ttccccccat ttttttccaa attccaaatt tttttcaaaa attttccagc gctaccgctc
     2821 ggcaaaattg caagcaattt ttaaaatcaa acccatgagg gaatttcatt ccctcaaact
     2881 cccttgagcc tcctccaacc gaaatagaag gacgctgcgc ttattatttc attcagtcat
     2941 cggctttcat aatctaacag acaacatctt cgctgcaaag cacgctacgc tcaagggctt
     3001 ttacgctacg ataacgcctg ttttaacgat tatgccgata actaaacgaa ataaacgcta
     3061 aaacgtctca gaaacgattt tgagacgttt taataaaaaa tcgaccgaaa aataaatata
     3121 aatcggatat acaatcggca attgacgaaa ctgcaaaata tcctgtaaag gatacggatt
     3181 ttatgaccga tgatgaagaa aagaatttga aacgtttgtc tgatttggag gaaggtttac
     3241 accgtaaaag gttaatctcc tatggtggtt tgttaaaaga aatacataaa aaattaaacc
     3301 ttgatgacac agaagaaggc gatttgattc atacagatga tgacgaaaaa gccgatgaag
     3361 atggattttc tattattgca atgtggaatt gggaacggaa aaattatttt attaaagagt
     3421 agttcaacaa acgggccagt ttgttgaaga ttagatgcta taattgttat taaaaggatt
     3481 gaaggatgct taggaagacg agttattaat agctgaataa gaacggtgct ctccaaatat
     3541 tcttatttag aaaacgaaat ctaaaattat ctgaaaaggg aatgagaata gtgaatggac
     3601 caataataat gactagagaa gaaagaatga agattgttca tgaaattaag gaacgaatat
     3661 tggataacat aaaaaacgcc cggcggcaac cgagcgttct gaaatatggg gatgatgtta
     3721 aggctattgg tgtttatggt aaaacgacgg ccagtcttat agaattcact agtaattgtg
     3781 aggcggataa caattctcac tcattctaca gtttattctt gacattgcac tgtccccctg
     3841 gtataataac taattgtgag cgctcacaat taagatcccc ggggcggccg cgaattcaaa
     3901 agatctaagg aggaacatat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc
     3961 tatcctctag aggatggaac cgctggagag caactgcata aggctatgaa gagatacgcc
     4021 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtgaacat cacgtacgcg
     4081 gaatacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca
     4141 aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc
     4201 gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg
     4261 ctcaacagta tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa ggggttgcaa
     4321 aaaattttga acgtgcaaaa aaaattacca ataatccaga aaattattat catggattct
     4381 aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc
     4441 ggttttaatg aatacgattt tgtaccagag tcctttgatc gtgacaaaac aattgcactg
     4501 ataatgaatt cctctggatc tactgggtta cctaagggtg tggcccttcc gcatagaact
     4561 gcctgcgtca gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat
     4621 actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga
     4681 tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgttttta
     4741 cgatcccttc aggattacaa aattcaaagt gcgttgctag taccaaccct attttcattc
     4801 ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct
     4861 gggggcgcac ctctttcgaa agaagtcggg gaagcggttg caaaacgctt ccatcttcca
     4921 gggatacgac aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag
     4981 ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg
     5041 gatctggata ccgggaaaac gctgggcgtt aatcagagag gcgaattatg tgtcagagga
     5101 cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag
     5161 gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca cttcttcata
     5221 gttgaccgct tgaagtcttt aattaaatac aaaggatatc aggtggcccc cgctgaattg
     5281 gaatcgatat tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg tcttcccgac
     5341 gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg
     5401 gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga
     5461 ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa
     5521 atcagagaga tcctcataaa ggccaagaag ggcggaaagt ccaaattgta atactagtac
     5581 tgcag
//
