LOCUS       pSpy1C-tt0-Pzn_f        5568 bp DNA     circular SYN 26-MAR-2024
DEFINITION  Restriction Cloning ligation product.
ACCESSION   urn.local...6w-dbyx147
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 26, 2024 from SnapGene Viewer 6.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3669..3703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3757..3891
                     /focus
                     /strain="SF370"
                     /serotype="M1"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:160490"
                     /organism="Streptococcus pyogenes M1 GAS"
     primer_bind     complement(5..21)
                     /label=M13_r
     terminator      352..394
                     /standard_name="predicted terminator"
                     /label=predicted terminator
     -35_signal      482..487
                     /standard_name="-35"
                     /label=-35
     -10_signal      505..510
                     /standard_name="-10"
                     /label=-10
     CDS             574..1221
                     /standard_name="Cm(R)"
                     /label=Cm(R)
     CDS             complement(1599..2294)
                     /standard_name="REP A"
                     /label=REP A
     CDS             complement(2363..2521)
                     /standard_name="REP C"
                     /label=REP C
     terminator      complement(3669..3703)
                     /standard_name="lambda t0 terminator"
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     primer_bind     3739..3756
                     /label=M13_f
     promoter        3757..3891
                     /standard_name="Pzn"
                     /label=PZn promoter
     regulatory      3777..3792
                     /standard_name="Operator"
                     /label=Operator
     -35_signal      3786..3791
     -10_signal      3806..3814
     gene            3892..5544
                     /standard_name="firefly luciferase"
                     /label=firefly luciferase
ORIGIN
        1 atgtgtcata gctgtttcct gcaaagaatg gtgatgacaa ttgtacaggt tatcaataaa
       61 gccattgata ctctcattct tatcccgtat ttattaactt ctcctagtat tcgtaattat
      121 catggctgtc atggcgcatt aacggaataa agggtgtgct taaatcgggc cattttgcgt
      181 aataagaaaa aggattaatt atgagcgaat tgaattaata ataaggtaat agatttacat
      241 tagaaaatga aaggggattt tatgcgtgag aatgttacag tctatcccgg caatagttac
      301 ccttattatc aagataagaa agaaaaggat ttttcgctac gctcaaatcc tttaaaaaaa
      361 cacaaaagac cacatttttt aatgtggtct ttattcttca actaaagcac ccattagttc
      421 aacaaacgaa aattggataa agtgggatat ttttaaaata tatatttatg ttacagtaat
      481 attgactttt aaaaaaggat tgattctaat gaagaaagca gacaagtaag cctcctaaat
      541 tcactttaga taaaaattta ggaggcatat caaatgaact ttaataaaat tgatttagac
      601 aattggaaga gaaaagagat atttaatcat tatttgaacc aacaaacgac ttttagtata
      661 accacagaaa ttgatattag tgttttatac cgaaacataa aacaagaagg atataaattt
      721 taccctgcat ttattttctt agtgacaagg gtgataaact caaatacagc ttttagaact
      781 ggttacaata gcgacggaga gttaggttat tgggataagt tagagccact ttatacaatt
      841 tttgatggtg tatctaaaac attctctggt atttggactc ctgtaaagaa tgacttcaaa
      901 gagttttatg atttatacct ttctgatgta gagaaatata atggttcggg gaaattgttt
      961 cccaaaacac ctatacctga aaatgctttt tctctttcta ttattccatg gacttcattt
     1021 actgggttta acttaaatat caataataat agtaattacc ttctacccat tattacagca
     1081 ggaaaattca ttaataaagg taattcaata tatttaccgc tatctttaca ggtacatcat
     1141 tctgtttgtg atggttatca tgcaggattg tttatgaact ctattcagga attgtcagat
     1201 aggcctaatg actggctttt ataatatgag ataatgccga ctgtactttt tacagtcggt
     1261 tttctaatgt cactaacctg ccccgttagt tgaagaaggt ttttatatta cagctccaga
     1321 tccatatcct tctttttctg aaccgacttc tcctttttcg cttctttatt ccaattgctt
     1381 tattgacgtt gagcctcgga acccttaaca atcccaaaac ttgtcgactt tcgtcagggg
     1441 ggcttttatt tattcaataa tccctcctct caataaatct attgttgtac ttaattcaac
     1501 ttccatttct ctgtatcttt caatacgctc ttttaagtcc ttaatttctt tttttaattc
     1561 ctcattttca gcaaataact ctttttcttt gtttgtcatt ttatttcccc cgtttcagca
     1621 tcaagaacct ttgcataact tgctctatat ccacactgat aattgccctc aaaccataat
     1681 ctaaaggcgc tagagtttgt tgaaacaata tcttttacat cattcgtatt taaaattcca
     1741 aactccgctc ccctaaggcg aataaaagcc attaaatctt ttgtatttac caaattatag
     1801 tcatccacta tatctaaaag taaattcttc aattctcttt tttggctttc atcaagtgtt
     1861 atatagcggt caatatcaaa atcattaatg ttcaaaatat cttttttgtc gtatatatgt
     1921 ttattcttag caatagcgtc ctttgattca tgagtcaaat attcatatga acctttgata
     1981 taatcaagta tctcaacatg agcaactgaa ctattcccca attttcgctt aatcttgttc
     2041 ctaacgcttt ctattgttac aggatttcgt gcaatatata taacgtgata gtgtggtttt
     2101 ttatagtgct ttccatttcg tataacatca ctactattcc atgtatcttt atcttttttt
     2161 tcgtccatat cgtgtaaagg actgacagcc atagatacgc ccaaactctc taatttttct
     2221 ttccaatcat taggaattga gtcaggatat aataaaaatc caaaatttct agctttagta
     2281 tttttaatag ccatgatata ttaccttatc aaaaacaagt agcgaaaact cgtatccttc
     2341 taaaaacgcg agctttcgct tatttttttt gttctgattc ctttcttgca tattcttcta
     2401 tagctaacgc cgcaaccgca gattttgaaa aacctttttg tttcgccata tctgttaatt
     2461 ttttatcttg ctcttttgtc agagaaatca taactctttt tttcgattct gaaatcacca
     2521 tttaaaaaac tccaatcaaa taattttata aaattagtgt atcactttgt aatcataaaa
     2581 acaacaataa agctacttaa atatagattt ataaaaaacg ttggcgaaaa cgttggcgat
     2641 tcgttggcga ttgaaaaacc cctcaaaccc ttgagccagt tgggatagag cgtttttggc
     2701 acaaaaattg gcactcggca cttaatgggg ggtcgtagta cggaagcaaa attcgcttcc
     2761 tttcccccca tttttttcca aattccaaat ttttttcaaa aattttccag cgctaccgct
     2821 cggcaaaatt gcaagcaatt tttaaaatca aacccatgag ggaatttcat tccctcaaac
     2881 tcccttgagc ctcctccaac cgaaatagaa ggacgctgcg cttattattt cattcagtca
     2941 tcggctttca taatctaaca gacaacatct tcgctgcaaa gcacgctacg ctcaagggct
     3001 tttacgctac gataacgcct gttttaacga ttatgccgat aactaaacga aataaacgct
     3061 aaaacgtctc agaaacgatt ttgagacgtt ttaataaaaa atcgaccgaa aaataaatat
     3121 aaatcggata tacaatcggc aattgacgaa actgcaaaat atcctgtaaa ggatacggat
     3181 tttatgaccg atgatgaaga aaagaatttg aaacgtttgt ctgatttgga ggaaggttta
     3241 caccgtaaaa ggttaatctc ctatggtggt ttgttaaaag aaatacataa aaaattaaac
     3301 cttgatgaca cagaagaagg cgatttgatt catacagatg atgacgaaaa agccgatgaa
     3361 gatggatttt ctattattgc aatgtggaat tgggaacgga aaaattattt tattaaagag
     3421 tagttcaaca aacgggccag tttgttgaag attagatgct ataattgtta ttaaaaggat
     3481 tgaaggatgc ttaggaagac gagttattaa tagctgaata agaacggtgc tctccaaata
     3541 ttcttattta gaaaacgaaa tctaaaatta tctgaaaagg gaatgagaat agtgaatgga
     3601 ccaataataa tgactagaga agaaagaatg aagattgttc atgaaattaa ggaacgaata
     3661 ttggataaat aaaaaacgcc cggcggcaac cgagcgttct gaaatatggg gatgatgtta
     3721 aggctattgg tgtttatggt aaaacgacgg ccagtcatcg gacactttct aaatgttgtt
     3781 cactattgaa caggtgctca tttttggtta tagtaattgt attttaactt ttagataata
     3841 gaataaagca ttctactata catattacca gtataacaaa aggagaaaat tatggaagac
     3901 gccaaaaaca taaagaaagg cccggcgcca ttctatcctc tagaggatgg aaccgctgga
     3961 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca
     4021 gatgcacata tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc cgttcggttg
     4081 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa
     4141 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg
     4201 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct
     4261 accgtagtgt ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta
     4321 ccaataatcc agaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg
     4381 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtacca
     4441 gagtcctttg atcgtgacaa aacaattgca ctgataatga attcctctgg atctactggg
     4501 ttacctaagg gtgtggccct tccgcataga actgcctgcg tcagattctc gcatgccaga
     4561 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc
     4621 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc
     4681 ttaatgtata gatttgaaga agagctgttt ttacgatccc ttcaggatta caaaattcaa
     4741 agtgcgttgc tagtaccaac cctattttca ttcttcgcca aaagcactct gattgacaaa
     4801 tacgatttat ctaatttaca cgaaattgct tctgggggcg cacctctttc gaaagaagtc
     4861 ggggaagcgg ttgcaaaacg cttccatctt ccagggatac gacaaggata tgggctcact
     4921 gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt
     4981 aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc
     5041 gttaatcaga gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg ttatgtaaac
     5101 aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata
     5161 gcttactggg acgaagacga acacttcttc atagttgacc gcttgaagtc tttaattaaa
     5221 tacaaaggat atcaggtggc ccccgctgaa ttggaatcga tattgttaca acaccccaac
     5281 atcttcgacg cgggcgtggc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc
     5341 gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc
     5401 agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg
     5461 aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag
     5521 aagggcggaa agtccaaatt gtaatactag tactgcagtg aaggtctc
//
