LOCUS       pJET1.2-OsMac3          3148 bp    DNA     circular SYN 10-DEC-2023
DEFINITION  Linearized positive selection cloning vector with a lethal insert that allows for efficient recovery of blunt-ended PCR products.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Thermo Fisher (Fermentas)
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Nov 27, 2019 from SnapGene Viewer 4.3.10 to 
            Vector NTI(R) format
            https://www.snapgene.com
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-15219480|
COMMENT     VNTDBDATE|-15219480|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pJET1.2-OsMac3|
COMMENT     VNTAUTHORNAME|Alex|
COMMENT     VNTOAUTHORNAME|Thermo Fisher (Fermentas)|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #2=(CFSignal (CObList) "pJET1.2 reverse sequencing primer" 27 0 1 208 
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COMMENT        #6=(CFSignal (CObList) "AmpR" 4 0 1 1725 2585 0 (CStringList) 
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COMMENT                          "DNA 'pJET1.2-OsMac3'" 1) 
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COMMENT                          "Linearized positive selection cloning vector with a lethal insert that allows for efficient recovery of blunt-ended PCR products." 
COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: Thermo Fisher (Fermentas)" 
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COMMENT                          "Created: 12/10/23 12:15" 1) 
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COMMENT                          "length: 3148 bp" 1) 
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COMMENT                          "Division: SYN" 1) 
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COMMENT                          "Modification Date in the Original DB: 19-OCT-2017" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Sequence Source: synthetic DNA construct" 1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/synthetic+DNA+construct\">synthetic DNA construct(synthetic DNA construct)</a>" 
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COMMENT                          "Associated Genes:  eco47IR;  bla" 1))) 
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COMMENT                          "REFERENCE   1  (bases 1 to 2974)" 1) 
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COMMENT                          "  AUTHORS   Thermo Fisher (Fermentas)" 1) 
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COMMENT                          "  TITLE     Direct Submission" 1) 
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COMMENT                          "  JOURNAL   Exported Wednesday, Nov 27, 2019 from SnapGene Viewer 4.3.10 to " 
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COMMENT                          "            Vector NTI(R) format" 1) 
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COMMENT                          "            https://www.snapgene.com" 1))) 
COMMENT                   "References") 
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COMMENT                                        "cleaves double-stranded DNA at GGWCC sites in the absence of protective methylation" 
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COMMENT                                        "confers resistance to ampicillin, carbenicillin, and related antibiotics" 
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COMMENT                                          "promoter for bacteriophage T7 RNA polymerase" 
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COMMENT                                           "The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." 
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COMMENT                                           "high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" 
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FEATURES             Location/Qualifiers
     CDS             complement(175..556)
                     /codon_start=1
                     /gene="eco47IR"
                     /product="mutant version of the Eco47I restriction
                     endonuclease"
                     /vntifkey="4"
                     /label=Eco47I/T7
                     /note="cleaves double-stranded DNA at GGWCC sites in the absence of protective methylation"
     misc_feature    175..225
                     /vntifkey="21"
                     /label=MCS
                     /note="multiple cloning site"
     primer          complement(208..231)
                     /vntifkey="27"
                     /label=pJET1.2\reverse\sequencing\primer
                     /note="sequence: AAGAACATCGATTTTCCATGGCAG"
     protein_bind    585..601
                     /bound_moiety="lac repressor encoded by lacI"
                     /vntifkey="31"
                     /label=lac\operator
                     /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(609..639)
                     /vntifkey="30"
                     /label=lac\UV5\promoter
                     /note="E. coli lac promoter with an   up   mutation"
     rep_origin      complement(966..1554)
                     /direction=LEFT
                     /vntifkey="33"
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1725..2585)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /vntifkey="4"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2586..2690)
                     /gene="bla"
                     /vntifkey="30"
                     /label=AmpR\promoter
     CDS             complement(2793..3148)
                     /codon_start=1
                     /gene="eco47IR"
                     /product="mutant version of the Eco47I restriction
                     endonuclease"
                     /vntifkey="4"
                     /label=Eco47I/T7
                     /note="cleaves double-stranded DNA at GGWCC sites in the absence of protective methylation"
     promoter        3082..3100
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer          3087..3109
                     /vntifkey="27"
                     /label=pJET1.2\forward\sequencing\primer
                     /note="sequence: CGACTCACTATAGGGAGAGCGGC"
     misc_feature    3105..3148
                     /vntifkey="21"
                     /label=MCS
                     /note="multiple cloning site"
     enhancer        complement(9..166)
                     /vntifkey="9"
                     /label=5'-UTR\of\OsMac3
BASE COUNT      826 a       734 c       689 g       899 t 
ORIGIN
        1 aatctagaat tgcgagacag tgccgtgggt aaatattgtg atctgttctc ttgagctgaa 
       61 attcctgcct ttcttcttct acaggctaga gagtcccagg attcagaaaa tgaggagcaa 
      121 atcttttgtt gtacttcttt ggtatgaaaa agctctcttt agtctttcta gattatcttt 
      181 ctagaagatc tcctacaata ttctcagctg ccatggaaaa tcgatgttct tcttttattc 
      241 tctcaagatt ttcaggctgt atattaaaac ttatattaag aactatgcta accacctcat 
      301 caggaaccgt tgtaggtggc gtgggttttc ttggcaatcg actctcatga aaactacgag 
      361 ctaaatattc aatatgttcc tcttgaccaa ctttattctg catttttttt gaacgaggtt 
      421 tagagcaagc ttcaggaaac tgagacagga attttattaa aaatttaaat tttgaagaaa 
      481 gttcagggtt aatagcatcc attttttgct ttgcaagttc ctcagcattc ttaacaaaag 
      541 acgtctcttt tgacatgttt aaagtttaaa cctcctgtgt gaaattgtta tccgctcaca 
      601 attccacaca ttatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 
      661 agctaactca cattaattgc gttgcgctca ctgccaattg ctttccagtc gggaaacctg 
      721 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 
      781 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 
      841 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 
      901 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
      961 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     1021 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     1081 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     1141 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     1201 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     1261 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     1321 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     1381 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 
     1441 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     1501 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     1561 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     1621 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     1681 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 
     1741 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 
     1801 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 
     1861 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 
     1921 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 
     1981 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 
     2041 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 
     2101 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 
     2161 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 
     2221 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 
     2281 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
     2341 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
     2401 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
     2461 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
     2521 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
     2581 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     2641 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     2701 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat 
     2761 aggcgtatca cgaggccgcc cctgcagccg aattatatta tttttgccaa ataattttta 
     2821 acaaaagctc tgaagtcttc ttcatttaaa ttcttagatg atacttcatc tggaaaattg 
     2881 tcccaattag tagcatcacg ctgtgagtaa gttctaaacc atttttttat tgttgtatta 
     2941 tctctaatct tactactcga tgagttttcg gtattatctc tatttttaac ttggagcagg 
     3001 ttccattcat tgtttttttc atcatagtga ataaaatcaa ctgctttaac acttgtgcct 
     3061 gaacaccata tccatccggc gtaatacgac tcactatagg gagagcggcc gccagatctt 
     3121 ccggatggct cgagtttttc agcaagat 
//
