LOCUS       BN698_AAV-SYN-NLS-SaCas9-NLS-3xHA-bGHpA-U6-BsaI-sgRNA.xdna    7341 bp      DNA     circular   25/03/2024
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     promoter        8..141
                     /label=5' ITR
                     /SerialCloner_Color=&hFC8008
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
                     /SerialCloner_Desc=vntifkey="30" - label=SynapsinIpromoter - ApEinfo_fwdcolor=#0080c0 - ApEinfo_revcolor=#0080c0 - 202..670 \ 
     promoter        146..617
                     /label=SYN promoter
                     /SerialCloner_Color=&h0000FE
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
                     /SerialCloner_Desc=vntifkey="30" - label=SynapsinIpromoter - ApEinfo_fwdcolor=#0080c0 - ApEinfo_revcolor=#0080c0 - 202..670 \ 
     misc_feature    651..659
                     /label=Kozak
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    663..686
                     /label=SV40 NLS
                     /SerialCloner_Color=&h803F02
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    708..3863
                     /label=SaCas9
                     /SerialCloner_Color=&h803F02
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3864..3911
                     /label=nucleoplasmin NLS
                     /SerialCloner_Color=&h803F02
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    3918..3998
                     /label=3xHA
                     /SerialCloner_Color=&h803F02
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4026..4239
                     /label=bGH PA terminator
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    4246..4487
                     /label=U6 promoter
                     /SerialCloner_Color=&h0000FF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4515..4590
                     /label=guide RNA scaffold for the Staphylococcus aureus _Sa gRNA scaffold
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    complement(4604..4744)
                     /label=3' ITR
                     /SerialCloner_Color=&hFF7F00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    4819..5274
                     /label=F1_origin
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    5556..5660
                     /label=AmpR promoter
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    5661..6521
                     /label=AmpR
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
     misc_feature    6692..7280
                     /label=ori
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=False
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCGTCG GGCGACCTTT 
       61 GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG GAGTGGCCAA CTCCATCACT 
      121 AGGGGTTCCT GCGGCCTCTA GACTCTAGCT GCAGAGGGAC CTGCGTATGA GTGCAAGTGG 
      181 GTTTTAGGAC CAGGATGAGG CGGGGTGGGG GTGCCTACCT GACGACCGAC CCCGACCCAC 
      241 TGGACAAGCA CCCAACCCCC ATTCCCCAAA TTGCGCATCC CCTATCAGAG AGGGGGAGGG 
      301 GAAACAGGAT GCGGCGAGGC GCGTGCGCAC TGCCAGCTTC AGCACCGCGG ACAGTGCCTT 
      361 CGCCCCCGCC TGGCGGCGCG CGCCACCGCC GCCTCAGCAC TGAAGGCGCG CTGACGTCAC 
      421 TCGCCGGTCC CCCGCAAACT CCCCTTCCCG GCCACCTTGG TCGCGTCCGC GCCGCCGCCG 
      481 GCCCAGCCGG ACCGCACCAC GCGAGGCGCG AGATAGGGGG GCACGGGCGC GACCATCTGC 
      541 GCTGCGGCGC CGGCGACTCA GCGCTGCCTC AGTCTGCGGT GGGCAGCGGA GGAGTCGTGT 
      601 CGTGCCTGAG AGCGCAGTCG AATTCAAGCT GCTAGCAAGG ATCCACCGGT GCCACCATGG 
      661 CCCCAAAGAA GAAGCGGAAG GTCGGTATCC ACGGAGTCCC AGCAGCCAAG CGGAACTACA 
      721 TCCTGGGCCT GGACATCGGC ATCACCAGCG TGGGCTACGG CATCATCGAC TACGAGACAC 
      781 GGGACGTGAT CGATGCCGGC GTGCGGCTGT TCAAAGAGGC CAACGTGGAA AACAACGAGG 
      841 GCAGGCGGAG CAAGAGAGGC GCCAGAAGGC TGAAGCGGCG GAGGCGGCAT AGAATCCAGA 
      901 GAGTGAAGAA GCTGCTGTTC GACTACAACC TGCTGACCGA CCACAGCGAG CTGAGCGGCA 
      961 TCAACCCCTA CGAGGCCAGA GTGAAGGGCC TGAGCCAGAA GCTGAGCGAG GAAGAGTTCT 
     1021 CTGCCGCCCT GCTGCACCTG GCCAAGAGAA GAGGCGTGCA CAACGTGAAC GAGGTGGAAG 
     1081 AGGACACCGG CAACGAGCTG TCCACCAAAG AGCAGATCAG CCGGAACAGC AAGGCCCTGG 
     1141 AAGAGAAATA CGTGGCCGAA CTGCAGCTGG AACGGCTGAA GAAAGACGGC GAAGTGCGGG 
     1201 GCAGCATCAA CAGATTCAAG ACCAGCGACT ACGTGAAAGA AGCCAAACAG CTGCTGAAGG 
     1261 TGCAGAAGGC CTACCACCAG CTGGACCAGA GCTTCATCGA CACCTACATC GACCTGCTGG 
     1321 AAACCCGGCG GACCTACTAT GAGGGACCTG GCGAGGGCAG CCCCTTCGGC TGGAAGGACA 
     1381 TCAAAGAATG GTACGAGATG CTGATGGGCC ACTGCACCTA CTTCCCCGAG GAACTGCGGA 
     1441 GCGTGAAGTA CGCCTACAAC GCCGACCTGT ACAACGCCCT GAACGACCTG AACAATCTCG 
     1501 TGATCACCAG GGACGAGAAC GAGAAGCTGG AATATTACGA GAAGTTCCAG ATCATCGAGA 
     1561 ACGTGTTCAA GCAGAAGAAG AAGCCCACCC TGAAGCAGAT CGCCAAAGAA ATCCTCGTGA 
     1621 ACGAAGAGGA TATTAAGGGC TACAGAGTGA CCAGCACCGG CAAGCCCGAG TTCACCAACC 
     1681 TGAAGGTGTA CCACGACATC AAGGACATTA CCGCCCGGAA AGAGATTATT GAGAACGCCG 
     1741 AGCTGCTGGA TCAGATTGCC AAGATCCTGA CCATCTACCA GAGCAGCGAG GACATCCAGG 
     1801 AAGAACTGAC CAATCTGAAC TCCGAGCTGA CCCAGGAAGA GATCGAGCAG ATCTCTAATC 
     1861 TGAAGGGCTA TACCGGCACC CACAACCTGA GCCTGAAGGC CATCAACCTG ATCCTGGACG 
     1921 AGCTGTGGCA CACCAACGAC AACCAGATCG CTATCTTCAA CCGGCTGAAG CTGGTGCCCA 
     1981 AGAAGGTGGA CCTGTCCCAG CAGAAAGAGA TCCCCACCAC CCTGGTGGAC GACTTCATCC 
     2041 TGAGCCCCGT CGTGAAGAGA AGCTTCATCC AGAGCATCAA AGTGATCAAC GCCATCATCA 
     2101 AGAAGTACGG CCTGCCCAAC GACATCATTA TCGAGCTGGC CCGCGAGAAG AACTCCAAGG 
     2161 ACGCCCAGAA AATGATCAAC GAGATGCAGA AGCGGAACCG GCAGACCAAC GAGCGGATCG 
     2221 AGGAAATCAT CCGGACCACC GGCAAAGAGA ACGCCAAGTA CCTGATCGAG AAGATCAAGC 
     2281 TGCACGACAT GCAGGAAGGC AAGTGCCTGT ACAGCCTGGA AGCCATCCCT CTGGAAGATC 
     2341 TGCTGAACAA CCCCTTCAAC TATGAGGTGG ACCACATCAT CCCCAGAAGC GTGTCCTTCG 
     2401 ACAACAGCTT CAACAACAAG GTGCTCGTGA AGCAGGAAGA AAACAGCAAG AAGGGCAACC 
     2461 GGACCCCATT CCAGTACCTG AGCAGCAGCG ACAGCAAGAT CAGCTACGAA ACCTTCAAGA 
     2521 AGCACATCCT GAATCTGGCC AAGGGCAAGG GCAGAATCAG CAAGACCAAG AAAGAGTATC 
     2581 TGCTGGAAGA ACGGGACATC AACAGGTTCT CCGTGCAGAA AGACTTCATC AACCGGAACC 
     2641 TGGTGGATAC CAGATACGCC ACCAGAGGCC TGATGAACCT GCTGCGGAGC TACTTCAGAG 
     2701 TGAACAACCT GGACGTGAAA GTGAAGTCCA TCAATGGCGG CTTCACCAGC TTTCTGCGGC 
     2761 GGAAGTGGAA GTTTAAGAAA GAGCGGAACA AGGGGTACAA GCACCACGCC GAGGACGCCC 
     2821 TGATCATTGC CAACGCCGAT TTCATCTTCA AAGAGTGGAA GAAACTGGAC AAGGCCAAAA 
     2881 AAGTGATGGA AAACCAGATG TTCGAGGAAA AGCAGGCCGA GAGCATGCCC GAGATCGAAA 
     2941 CCGAGCAGGA GTACAAAGAG ATCTTCATCA CCCCCCACCA GATCAAGCAC ATTAAGGACT 
     3001 TCAAGGACTA CAAGTACAGC CACCGGGTGG ACAAGAAGCC TAATAGAGAG CTGATTAACG 
     3061 ACACCCTGTA CTCCACCCGG AAGGACGACA AGGGCAACAC CCTGATCGTG AACAATCTGA 
     3121 ACGGCCTGTA CGACAAGGAC AATGACAAGC TGAAAAAGCT GATCAACAAG AGCCCCGAAA 
     3181 AGCTGCTGAT GTACCACCAC GACCCCCAGA CCTACCAGAA ACTGAAGCTG ATTATGGAAC 
     3241 AGTACGGCGA CGAGAAGAAT CCCCTGTACA AGTACTACGA GGAAACCGGG AACTACCTGA 
     3301 CCAAGTACTC CAAAAAGGAC AACGGCCCCG TGATCAAGAA GATTAAGTAT TACGGCAACA 
     3361 AACTGAACGC CCATCTGGAC ATCACCGACG ACTACCCCAA CAGCAGAAAC AAGGTCGTGA 
     3421 AGCTGTCCCT GAAGCCCTAC AGATTCGACG TGTACCTGGA CAATGGCGTG TACAAGTTCG 
     3481 TGACCGTGAA GAATCTGGAT GTGATCAAAA AAGAAAACTA CTACGAAGTG AATAGCAAGT 
     3541 GCTATGAGGA AGCTAAGAAG CTGAAGAAGA TCAGCAACCA GGCCGAGTTT ATCGCCTCCT 
     3601 TCTACAACAA CGATCTGATC AAGATCAACG GCGAGCTGTA TAGAGTGATC GGCGTGAACA 
     3661 ACGACCTGCT GAACCGGATC GAAGTGAACA TGATCGACAT CACCTACCGC GAGTACCTGG 
     3721 AAAACATGAA CGACAAGAGG CCCCCCAGGA TCATTAAGAC AATCGCCTCC AAGACCCAGA 
     3781 GCATTAAGAA GTACAGCACA GACATTCTGG GCAACCTGTA TGAAGTGAAA TCTAAGAAGC 
     3841 ACCCTCAGAT CATCAAAAAG GGCAAAAGGC CGGCGGCCAC GAAAAAGGCC GGCCAGGCAA 
     3901 AAAAGAAAAA GGGATCCTAC CCATACGATG TTCCAGATTA CGCTTACCCA TACGATGTTC 
     3961 CAGATTACGC TTACCCATAC GATGTTCCAG ATTACGCTTA AGAATTCCTA GAGCTCGCTG 
     4021 ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC 
     4081 TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC 
     4141 ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA 
     4201 GGGGGAGGAT TGGGAAGAGA ATAGCAGGCA TGCTGGGGAG GTACCGAGGG CCTATTTCCC 
     4261 ATGATTCCTT CATATTTGCA TATACGATAC AAGGCTGTTA GAGAGATAAT TGGAATTAAT 
     4321 TTGACTGTAA ACACAAAGAT ATTAGTACAA AATACGTGAC GTAGAAAGTA ATAATTTCTT 
     4381 GGGTAGTTTG CAGTTTTAAA ATTATGTTTT AAAATGGACT ATCATATGCT TACCGTAACT 
     4441 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAA CACCGGAGAC 
     4501 CACGGCAGGT CTCAGTTTTA GTACTCTGGA AACAGAATCT ACTAAAACAA GGCAAAATGC 
     4561 CGTGTTTATC TCGTCAACTT GTTGGCGAGA TTTTTGCGGC CGCAGGAACC CCTAGTGATG 
     4621 GAGTTGGCCA CTCCCTCTCT GCGCGCTCGC TCGCTCACTG AGGCCGGGCG ACCAAAGGTC 
     4681 GCCCGACGCC CGGGCTTTGC CCGGGCGGCC TCAGTGAGCG AGCGAGCGCG CAGCTGCCTG 
     4741 CAGGGGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA 
     4801 CGTCAAAGCA ACCATAGTAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG 
     4861 TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCTTAGC GCCCGCTCCT TTCGCTTTCT 
     4921 TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC 
     4981 CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTTGGGTG 
     5041 ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT 
     5101 CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC TCTATCTCGG 
     5161 GCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGT CTATTGGTTA AAAAATGAGC 
     5221 TGATTTAACA AAAATTTAAC GCGAATTTTA ACAAAATATT AACGTTTACA ATTTTATGGT 
     5281 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA 
     5341 CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG 
     5401 TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA 
     5461 GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT 
     5521 CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT 
     5581 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 
     5641 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 
     5701 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 
     5761 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 
     5821 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 
     5881 TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 
     5941 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 
     6001 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 
     6061 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 
     6121 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 
     6181 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 
     6241 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 
     6301 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 
     6361 GAGCCGGTGA GCGTGGAAGC CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 
     6421 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 
     6481 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 
     6541 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 
     6601 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 
     6661 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 
     6721 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 
     6781 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC 
     6841 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 
     6901 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 
     6961 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 
     7021 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 
     7081 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 
     7141 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 
     7201 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 
     7261 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 
     7321 GCTGGCCTTT TGCTCACATG T
//