LOCUS       Exported                6436 bp ds-DNA     circular SYN 22-MAR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 25, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             730..822
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
     protein_bind    835..856
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        871..901
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    909..925
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     repeat_region   989..1129
                     /label=AAV2 ITR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=repeat_region"
     polyA_signal    1215..1282
                     /label=SV40 poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=82.9"
                     /note="/other=polyA_signal"
     polyA_signal    1286..1329
                     /label=SV40 poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=53.7"
                     /note="/other=polyA_signal"
     misc_feature    complement(1328..1412)
                     /label=WPRE (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=14.4"
                     /note="/other=misc_feature"
     misc_feature    complement(1418..1575)
                     /label=WPRE (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=96.2"
                     /note="/match_length=39.1"
                     /note="/other=misc_feature"
     protein_bind    1640..1673
                     /label=FRT (minimal)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             1794..2513
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     protein_bind    complement(2528..2561)
                     /label=loxP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     ncRNA           2601..2650
                     /label=Hammerhead 1
                     /note="pLannotate"
                     /note="/database=Rfam"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=108.7"
                     /note="/other=ncRNA"
     protein_bind    complement(2654..2687)
                     /label=loxP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     protein_bind    complement(2700..2747)
                     /label=FRT
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     intron          complement(2931..3947)
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=intron"
     promoter        complement(3948..4223)
                     /label=chicken beta-actin promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.3"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     enhancer        complement(4225..4528)
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     repeat_region   4649..4676
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=19.9"
                     /note="/other=repeat_region"
     repeat_region   4705..4732
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=19.9"
                     /note="/other=repeat_region"
     CDS             4765..4922
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=90.8"
                     /note="/other=CDS"
     misc_feature    complement(4928..5027)
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=70.9"
                     /note="/other=misc_feature"
     CDS             complement(5129..5164)
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
     promoter        5259..5363
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             5364..6224
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     rep_origin      join(6395..6436,1..547)
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
ORIGIN
        1 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
       61 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
      121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
      181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
      241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
      301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
      361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
      421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
      481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
      541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
      601 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
      661 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
      721 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      781 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      841 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      901 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      961 caagctcggc gcgccattgg gatccaatcc tgcaggcagc tgcgcgctcg ctcgctcact
     1021 gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc
     1081 gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctg gagctccggt
     1141 taccggccgc tttaaaaaac ctcccacatc tccccctgaa cctgaaacat aaaatgaatg
     1201 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1261 tcacaaattt cacaaataaa gctagatggt tacaaataaa gcaatagcat cacaaatttc
     1321 acaaataaac accacggaat tgtcagtgcc caacagccga gcccctgtcc agcagcgggc
     1381 aaggcaggcg gcgatgagtt ccgccgtggc aagaactaac caggatttat acaaggagga
     1441 gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc
     1501 acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta
     1561 atccagaggt tgattatctc gggcatcgaa tatcgaatcg gctaagctct agaaagcttc
     1621 gtacggaagt tactattccg aagttcctat tctctagaaa gtataggaac ttcgtgtcga
     1681 tggtgatgct tggcaattcg ggtcgatggt gaagcattgg tcttcctatt ttgatgaagt
     1741 tcctattctt caaaaggtat aggaacttcc tcgagaccgg tactagtgcc accatggtga
     1801 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     1861 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     1921 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     1981 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     2041 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     2101 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     2161 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     2221 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     2281 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     2341 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     2401 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     2461 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taagcatgca
     2521 tgaattcata acttcgtata atgtatgcta tacgaagtta ttttctttct ttgcgcgtcc
     2581 tggattcgcg gaaacgcgta catccagctg acgagtccca aataggacga aacgcgcttt
     2641 tctttttgtt tttataactt cgtataatgt atgctatacg aagttatctt aaggtcgacg
     2701 aagttcctat actttctaga gaataggaac ttcggaatag gaacttcgtg tcgatggtga
     2761 tgcttggcaa ttcgggtcga tggtgaagca ttggtcttcc tattttgatg aagttcctat
     2821 accttttgaa gaataggaac ttcgctagcg gtacccccgg gcccgcggta tcaaatcaat
     2881 tctttgccaa aatgatgaga cagcacaata accagcacgt tgcccaggag ctgtaggaaa
     2941 aagaagaagg catgaacatg gttagcagag gctctagagc cgccggtcac acgccagaag
     3001 ccgaaccccg ccctgccccg tcccccccga aggcagccgt ccccccgcgg acagccccga
     3061 ggctggagag ggagaagggg acggcggcgc ggcgacgcac gaaggccctc cccgcccatt
     3121 tccttcctgc cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt gcggcggcgc
     3181 ctcccagatt tcggctccgc acagatttgg gacaaaggaa gtccctgcgc cctctcgcac
     3241 gattaccata aaaggcaatg gctgcggctc gccgcgcctc gacagccgcc ggcgctccgg
     3301 gggccgccgc gcccctcccc cgagccctcc ccggcccgag gcggccccgc cccgcccggc
     3361 acccccacct gccgccaccc cccgcccggc acggcgagcc ccgcgccacg ccccgtacgg
     3421 agccccgcac ccgaagccgg gccgtgctca gcaactcggg gaggggggtg cagggggggt
     3481 tgcagcccga ccgacgcgcc cacaccccct gctcaccccc ccacgcacac accccgcacg
     3541 cagcctttgt tcccctcgca gccccccccg caccgcgggg caccgccccc ggccgcgctc
     3601 ccctcgcgca cactgcggag cgcacaaagc cccgcgccgc gcccgcagcg ctcacagccg
     3661 ccgggcagcg cggagccgca cgcggcgctc cccacgcaca cacacacgca cgcacccccc
     3721 gagccgctcc ccccgcacaa agggccctcc cggagcccct caaggctttc acgcagccac
     3781 agaaaagaaa caagccgtca ttaaaccaag cgctaattac agcccggagg agaagggccg
     3841 tcccgcccgc tcacctgtgg gagtaacgcg gtcagtcaga gccggggcgg gcggcgcgag
     3901 gcggcggcgg agcggggcac ggggcgaagg cagcgcgcag cgactcccgc ccgccgcgcg
     3961 cttcgctttt tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa ctttcggagc
     4021 gcgccgctct gattggctgc cgccgcacct ctccgcctcg ccccgccccg cccctcgccc
     4081 cgccccgccc cgcctggcgc gcgccccccc cccccccgcc cccatcgctg cacaaaataa
     4141 ttaaaaaata aataaataca aaattggggg tggggagggg ggggagatgg ggagagtgaa
     4201 gcagaacgtg gggctcacct cgaccatggt aatagcgatg actaatacgt agatgtactg
     4261 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
     4321 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
     4381 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
     4441 tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
     4501 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
     4561 ccccgtaatt gattactatt aataactaga ttcctgcaga cgcgtgcggc cgcaggaacc
     4621 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     4681 accaaaggtc gcccgacgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     4741 aggattggat cccaatacgc gtcaattcac tggccgtcgt tttacaacgt cgtgactggg
     4801 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     4861 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     4921 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     4981 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     5041 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     5101 ctgtgacagt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     5161 cgagacaaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5221 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5281 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5341 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5401 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5461 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5521 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5581 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5641 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5701 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5761 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5821 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5881 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5941 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6001 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6061 ctggagccgg tgagcgtggt tctcgcggta tcattgcagc actggggcca gatggtaagc
     6121 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6181 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6241 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6301 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6361 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6421 tctgctgctt gcaaac
//
