LOCUS       Exported                6282 bp ds-DNA     circular SYN 28-MAR-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Mar 25, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   13..153
                     /label=AAV2 ITR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=repeat_region"
     repeat_region   24..153
                     /label=AAV2 ITR
     misc_feature    complement(173..307)
                     /label=SL (estimated)
     polyA_signal    239..306
                     /label=SV40 poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=82.9"
                     /note="/other=polyA_signal"
     misc_feature    complement(261..304)
                     /label=SV40 late polyA
     polyA_signal    310..353
                     /label=SV40 poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=53.7"
                     /note="/other=polyA_signal"
     misc_feature    complement(310..353)
                     /label=SV40 late polyA
     misc_feature    complement(352..436)
                     /label=WPRE (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=14.4"
                     /note="/other=misc_feature"
     misc_feature    complement(356..598)
                     /label=wPRE3 (estimated)
     misc_feature    complement(442..599)
                     /label=WPRE (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=96.2"
                     /note="/match_length=39.1"
                     /note="/other=misc_feature"
     protein_bind    650..697
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    664..697
                     /label=FRT (minimal)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    760..793
                     /label=FRT5
     misc_feature    760..793
                     /label=FRT5
     misc_feature    812..817
                     /label=KOZAK
     CDS             818..1771
                     /codon_start=1
                     /label=StGtACR1 (assumed)
                     /translation="MSSITCDPAIYGEWSRENQFCVEKSLITLDGIKYVQLVMAVVSAC
                     QVFFMVTRAPKVPWEAIYLPTTEMITYSLAFTGNGYIRVANGKYLPWARMASWLCTCPI
                     MLGLVSNMALVKYKSIPLNPMMIAASSICTVFGITASVVLDPLHVWLYCFISSIFFIFE
                     MVVAFAIFAITIHDFQTIGSPMSLKVVERLKLMRIVFYVSWMAYPILWSFSSTGACIMS
                     ENTSSVLYLLGDALCKNTYGILLWATTWGLLNGKWDRDYVKGRNVDGTLMPEYEQDLEK
                     GNTERYEDARAGETAAKSRITSEGEYIPLDQIDINVV"
     CDS             1709..1768
                     /codon_start=1
                     /label=KCNJ2 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=4.7"
                     /note="/other=CDS"
                     /translation="KSRITSEGEYIPLDQIDINV"
     CDS             1772..2467
                     /codon_start=1
                     /label=FusionRed
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MVSELIKENMLMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     misc_feature    complement(2419..2424)
                     /label=KOZAK
     misc_feature    complement(2444..2449)
                     /label=KOZAK
     CDS             2468..2683
                     /codon_start=1
                     /label=KCNB1 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=84.7"
                     /note="/match_length=8.4"
                     /note="/other=CDS"
                     /translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
                     MSSIDSFISCATDFPEATRFK*EFITS"
     misc_feature    2633..2638
                     /label=KOZAK
     protein_bind    complement(2675..2708)
                     /label=loxP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    2709..2800
                     /label=T3H48 ribozyme
     ncRNA           2748..2797
                     /label=Hammerhead 1
                     /note="pLannotate"
                     /note="/database=Rfam"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=108.7"
                     /note="/other=ncRNA"
     protein_bind    complement(2801..2834)
                     /label=loxP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     protein_bind    complement(2847..2894)
                     /label=FRT
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    complement(2957..2990)
                     /label=FRT5
     misc_feature    complement(2957..2990)
                     /label=FRT5
     promoter        complement(3003..3450)
                     /label=hSyn promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    3484..3618
                     /label=AAV2 ITR (alternate)
     repeat_region   3519..3546
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=19.9"
                     /note="/other=repeat_region"
     repeat_region   3575..3602
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=19.9"
                     /note="/other=repeat_region"
     CDS             3635..3792
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=90.8"
                     /note="/other=CDS"
                     /translation="IHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPN
                     SCAA*MA"
     primer_bind     complement(3639..3655)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(3798..3897)
                     /label=bom (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=70.9"
                     /note="/other=misc_feature"
     CDS             complement(3999..4034)
                     /codon_start=1
                     /label=rop (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=18.8"
                     /note="/other=CDS"
                     /translation="LARFGDDGENL"
     misc_feature    complement(4105..4110)
                     /label=KOZAK
     promoter        4129..4233
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             4234..5094
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5265..5853
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     misc_feature    5435..5440
                     /label=KOZAK
     misc_feature    5792..5797
                     /label=KOZAK
     CDS             6036..6128
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    6141..6162
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        6177..6207
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    6215..6231
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     primer_bind     6239..6255
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttgggatcca atcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa
       61 agcccgggcg tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag
      121 agggagtggc caactccatc actaggggtt cctggagctc cggttaccgg ccgctttaaa
      181 aaacctccca catctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
      241 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
      301 taaagctaga tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaacaccacg
      361 gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcgatg
      421 agttccgccg tggcaagaac taaccaggat ttatacaagg aggagaaaat gaaagccata
      481 cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga
      541 gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgatta
      601 tctcgggcat cgaatatcga atcggctaag ctctagaaag cttcgtacgg aagttactat
      661 tccgaagttc ctattctcta gaaagtatag gaacttcgtg tcgatggtga tgcttggcaa
      721 ttcgggtcga tggtgaagca ttggtcttcc tattttgatg aagttcctat tcttcaaaag
      781 gtataggaac ttcctcgaga ccggtactag tgccaccatg agcagcatta cttgtgaccc
      841 agccatctat ggagagtgga gcagagagaa ccagttttgc gtcgaaaaga gcctgattac
      901 cctggacggc atcaagtacg tgcagctggt catggccgtg gtgagcgcct gccaggtgtt
      961 ctttatggtc actagggcac ccaaagtgcc ttgggaggcc atctacctgc caaccacaga
     1021 aatgatcacc tattcactgg ctttcacagg caacggatac attcgagtgg caaatgggaa
     1081 gtatctgcct tgggctagaa tggcaagttg gctgtgcaca tgtccaatca tgctgggcct
     1141 ggtgagcaac atggccctgg tgaagtacaa atccattcca ctgaatccca tgatgattgc
     1201 cgccagcagc atctgcacag tgttcggaat cactgcctcc gtggtcctgg accccctgca
     1261 cgtgtggctg tactgtttca tctctagtat tttctttatc tttgagatgg tggtcgcatt
     1321 cgccattttt gccatcacta ttcatgattt tcagaccatc ggaagtccca tgtcactgaa
     1381 ggtggtcgaa aggctgaaac tgatgagaat cgtgttctac gtgagctgga tggcctatcc
     1441 tatcctgtgg agtttttcaa gcacaggggc ttgcattatg tctgagaaca cttcctctgt
     1501 gctgtacctg ctgggcgacg cactgtgcaa aaatacctat ggaatcctgc tgtgggctac
     1561 tacctgggga ctgctgaacg gcaagtggga ccgagattac gtgaaaggac gcaatgtcga
     1621 tgggacactg atgcccgaat atgaacagga tctggagaaa ggcaatactg aacgatatga
     1681 ggatgctcgg gctggggaaa ccgccgccaa gagcaggatc accagcgagg gcgagtacat
     1741 ccccctggac cagatcgaca tcaacgtggt gatggtgagc gagctgatta aggagaacat
     1801 gctcatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
     1861 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtcg tcgagggcgg
     1921 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcagaacctt
     1981 catcaagcac cctccgggca tccccgactt ctttaagcag tccttccctg agggcttcac
     2041 atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag
     2101 cctccaggac ggctgcctca tctacaacgt caaggttaga ggggtgaact tcccagccaa
     2161 cggccctgtg atgcagaaga aaacactcgg ctgggaggcc tccaccgaga cgatgtaccc
     2221 tgctgacgga ggactggaag gcgcatgtga catggctctg aagctcgtgg gaggaggaca
     2281 cctgatctgc aaccttgaga caacatacag atccaagaaa cccgctacga acctcaagat
     2341 gcccggcgtc tacaacgtgg accacagact ggaaagaatc aaggaggccg acgatgagac
     2401 atacgtcgag cagcacgagg tggctgtggc cagatactct actggtggcg ctggtgatgg
     2461 aggtaaacag tcccagccta ttctgaacac caaagagatg gccccccaga gtaaaccccc
     2521 tgaggagctg gaaatgtcct caatgccctc ccctgtggca cctcttcctg caagaacaga
     2581 gggcgtgatc gacatgaggt ccatgagttc tattgactct tttatctcat gcgccaccga
     2641 ttttccagag gctacgcggt ttaagtaaga attcataact tcgtataatg tatgctatac
     2701 gaagttattt tctttctttg cgcgtcctgg attcgcggaa acgcgtacat ccagctgacg
     2761 agtcccaaat aggacgaaac gcgcttttct ttttgttttt ataacttcgt ataatgtatg
     2821 ctatacgaag ttatcttaag gtcgacgaag ttcctatact ttctagagaa taggaacttc
     2881 ggaataggaa cttcgtgtcg atggtgatgc ttggcaattc gggtcgatgg tgaagcattg
     2941 gtcttcctat tttgatgaag ttcctatacc ttttgaagaa taggaacttc gctagcggta
     3001 ccctgcgctc tcaggcacga cacgactcct ccgctgccca ccgcagactg aggcagcgct
     3061 gagtcgccgg cgccgcagcg cagatggtcg cgcccgtgcc cccctatctc gcgcctcgcg
     3121 tggtgcggtc cggctgggcc ggcggcggcg cggacgcgac caaggtggcc gggaagggga
     3181 gtttgcgggg gaccggcgag tgacgtcagc gcgccttcag tgctgaggcg gcggtggcgc
     3241 gcgccgccag gcgggggcga aggcactgtc cgcggtgctg aagctggcag tgcgcacgcg
     3301 cctcgccgca tcctgtttcc cctccccctc tctgataggg gatgcgcaat ttggggaatg
     3361 ggggttgggt gcttgtccag tgggtcgggg tcggtcgtca ggtaggcacc cccaccccgc
     3421 ctcatcctgg tcctaaaacc cacttgcact gcgatcgcgg gcccgtgcag catctgcggc
     3481 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     3541 aggccgggcg accaaaggtc gcccgacgcc cgggcggcct cagtgagcga gcgagcgcgc
     3601 agctgcctgc aggattggat cccaatacgc gtcaattcac tggccgtcgt tttacaacgt
     3661 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     3721 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     3781 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     3841 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     3901 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     3961 tacagacaag ctgtgacagt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     4021 ccgaaacgcg cgagacaaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     4081 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     4141 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4201 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     4261 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     4321 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     4381 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     4441 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     4501 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     4561 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     4621 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     4681 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     4741 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     4801 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     4861 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     4921 gctgataaat ctggagccgg tgagcgtggt tctcgcggta tcattgcagc actggggcca
     4981 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5041 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5101 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5161 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5221 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5281 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5341 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5401 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5461 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5521 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5581 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5641 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5701 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5761 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5821 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5881 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5941 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6001 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     6061 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     6121 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     6181 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     6241 ggaaacagct atgaccatga ttacgccaag ctcggcgcgc ca
//
