LOCUS       pKA07_1                 9626 bp    DNA     circular SYN 26-JUN-2024
DEFINITION  pKA07_1.
ACCESSION   pKA07_1
VERSION     pKA07_1
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      8010..8438
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             3636..7553
                     /note="pLannotate"
                     /label="AsCpf1"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="99.9"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1016..1207)
                     /note="pLannotate"
                     /label="rop"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(2016..3098)
                     /note="pLannotate"
                     /label="lacI"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        8491..8595
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             8596..9456
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             7608..7688
                     /note="pLannotate"
                     /label="3xHA"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(3099..3176)
                     /note="pLannotate"
                     /label="lacI promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             7554..7601
                     /note="pLannotate"
                     /label="nucleoplasmin NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      7926..7973
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     protein_bind    3504..3528
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     RBS             3543..3565
                     /note="pLannotate"
                     /label="RBS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
     promoter        3485..3503
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(775..914)
                     /note="pLannotate"
                     /label="bom"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             complement(1676..1967)
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="24.5"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3184..3463)
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="23.5"
                     /fragment="True"
                     /other="CDS"
     CDS             1224..1604
                     /note="pLannotate"
                     /label="YPB1_ECOLX"
                     /database="swissprot"
                     /identity="99.2"
                     /match_length="96.2"
                     /fragment="False"
                     /other="CDS"
     protein_bind    complement(1982..2003)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="95.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             7869..7918
                     /note="pLannotate"
                     /label="SET1 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="39.7"
                     /fragment="True"
                     /other="CDS"
     CDS             7842..7859
                     /note="pLannotate"
                     /label="9xHis (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="66.7"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg
     1681 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     1741 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     1801 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     1861 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     1921 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct
     1981 aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2041 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2101 ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc
     2161 accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga
     2221 aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg
     2281 tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt
     2341 gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc
     2401 agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct
     2461 atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc
     2521 gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga
     2581 tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc
     2641 tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg
     2701 gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga
     2761 ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg
     2821 ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc
     2881 agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt
     2941 gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt
     3001 ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg
     3061 gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc
     3121 tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg
     3181 atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag
     3241 gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     3301 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     3361 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     3421 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
     3481 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
     3541 attttgttta actttaagaa ggagatatac atatgaaacg taccgccgac ggcagcgagt
     3601 tcgagagccc gaaaaagaaa cgcaaagtgg gatcaacaca gttcgagggc tttaccaacc
     3661 tgtatcaggt gagcaagaca ctgcggtttg agctgatccc acagggcaag accctgaagc
     3721 acatccagga gcagggcttc atcgaggagg acaaggcccg caatgatcac tacaaggagc
     3781 tgaagcccat catcgatcgg atctacaaga cctatgccga ccagtgcctg cagctggtgc
     3841 agctggattg ggagaacctg agcgccgcca tcgactccta tagaaaggag aaaaccgagg
     3901 agacaaggaa cgccctgatc gaggagcagg ccacatatcg caatgccatc cacgactact
     3961 tcatcggccg gacagacaac ctgaccgatg ccatcaataa gagacacgcc gagatctaca
     4021 agggcctgtt caaggccgag ctgtttaatg gcaaggtgct gaagcagctg ggcaccgtga
     4081 ccacaaccga gcacgagaac gccctgctgc ggagcttcga caagtttaca acctacttct
     4141 ccggctttta tgagaacagg aagaacgtgt tcagcgccga ggatatcagc acagccatcc
     4201 cacaccgcat cgtgcaggac aacttcccca agtttaagga gaattgtcac atcttcacac
     4261 gcctgatcac cgccgtgccc agcctgcggg agcactttga gaacgtgaag aaggccatcg
     4321 gcatcttcgt gagcacctcc atcgaggagg tgttttcctt ccctttttat aaccagctgc
     4381 tgacacagac ccagatcgac ctgtataacc agctgctggg aggaatctct cgggaggcag
     4441 gcaccgagaa gatcaagggc ctgaacgagg tgctgaatct ggccatccag aagaatgatg
     4501 agacagccca catcatcgcc tccctgccac acagattcat ccccctgttt aagcagatcc
     4561 tgtccgatag gaacaccctg tctttcatcc tggaggagtt taagagcgac gaggaagtga
     4621 tccagtcctt ctgcaagtac aagacactgc tgagaaacga gaacgtgctg gagacagccg
     4681 aggccctgtt taacgagctg aacagcatcg acctgacaca catcttcatc agccacaaga
     4741 agctggagac aatcagcagc gccctgtgcg accactggga tacactgagg aatgccctgt
     4801 atgagcggag aatctccgag ctgacaggca agatcaccaa gtctgccaag gagaaggtgc
     4861 agcgcagcct gaagcacgag gatatcaacc tgcaggagat catctctgcc gcaggcaagg
     4921 agctgagcga ggccttcaag cagaaaacca gcgagatcct gtcccacgca cacgccgccc
     4981 tggatcagcc actgcctaca accctgaaga agcaggagga gaaggagatc ctgaagtctc
     5041 agctggacag cctgctgggc ctgtaccacc tgctggactg gtttgccgtg gatgagtcca
     5101 acgaggtgga ccccgagttc tctgcccggc tgaccggcat caagctggag atggagcctt
     5161 ctctgagctt ctacaacaag gccagaaatt atgccaccaa gaagccctac tccgtggaga
     5221 agttcaagct gaactttcag cggcctacac tggcctctgg ctgggacgtg aataaggaga
     5281 agaacaatgg cgccatcctg tttgtgaaga acggcctgta ctatctgggc atcatgccaa
     5341 agcagaaggg caggtataag gccctgagct tcgagcccac agagaaaacc agcgagggct
     5401 ttgataagat gtactatgac tacttccctg atgccgccaa gatgatccca aagtgcagca
     5461 cccagctgaa ggccgtgaca gcccactttc agacccacac aacccccatc ctgctgtcca
     5521 acaatttcat cgagcctctg gagatcacaa aggagatcta cgacctgaac aatcctgaga
     5581 aggagccaaa gaagtttcag acagcctacg ccaagaaaac cggcgaccag aagggctaca
     5641 gagaggccct gtgcaagtgg atcgacttca caagggattt tctgtccaag tataccaaga
     5701 caacctctat cgatctgtct agcctgcggc catcctctca gtataaggac ctgggcgagt
     5761 actatgccga gctgaatccc ctgctgtacc acatcagctt ccagagaatc gccgagaagg
     5821 agatcatgga tgccgtggag acaggcaagc tgtacctgtt ccagatctat aacaaggact
     5881 ttgccaaggg ccaccacggc aagcctaatc tgcacacact gtattggacc ggcctgtttt
     5941 ctccagagaa cctggccaag acaagcatca agctgaatgg ccaggccgag ctgttctacc
     6001 gccctaagtc caggatgaag aggatggcac accggctggg agagaagatg ctgaacaaga
     6061 agctgaagga tcagaaaacc ccaatccccg acaccctgta ccaggagctg tacgactatg
     6121 tgaatcacag actgtcccac gacctgtctg atgaggccag ggccctgctg cccaacgtga
     6181 tcaccaagga ggtgtctcac gagatcatca aggataggcg ctttaccagc gacaagttcc
     6241 ttttccacgt gcctatcaca ctgaactatc aggccgccaa ttccccatct aagttcaacc
     6301 agagggtgaa tgcctacctg aaggagcacc ccgagacacc tatcatcggc atcgatcggg
     6361 gcgagagaaa cctgatctat atcacagtga tcgactccac cggcaagatc ctggagcagc
     6421 ggagcctgaa caccatccag cagtttgatt accagaagaa gctggacaac agggagaagg
     6481 agagggtggc agcaaggcag gcctggtctg tggtgggcac aatcaaggat ctgaagcagg
     6541 gctatctgag ccaggtcatc cacgagatcg tggacctgat gatccactac caggccgtgg
     6601 tggtgctgga gaacctgaat ttcggcttta agagcaagag gaccggcatc gccgagaagg
     6661 ccgtgtacca gcagttcgag aagatgctga tcgataagct gaattgcctg gtgctgaagg
     6721 actatccagc agagaaagtg ggaggcgtgc tgaacccata ccagctgaca gaccagttca
     6781 cctcctttgc caagatgggc acccagtctg gcttcctgtt ttacgtgcct gccccatata
     6841 catctaagat cgatcccctg accggcttcg tggacccctt cgtgtggaaa accatcaaga
     6901 atcacgagag ccgcaagcac ttcctggagg gcttcgactt tctgcactac gacgtgaaaa
     6961 ccggcgactt catcctgcac tttaagatga acagaaatct gtccttccag aggggcctgc
     7021 ccggctttat gcctgcatgg gatatcgtgt tcgagaagaa cgagacacag tttgacgcca
     7081 agggcacccc tttcatcgcc ggcaagagaa tcgtgccagt gatcgagaat cacagattca
     7141 ccggcagata ccgggacctg tatcctgcca acgagctgat cgccctgctg gaggagaagg
     7201 gcatcgtgtt cagggatggc tccaacatcc tgccaaagct gctggagaat gacgattctc
     7261 acgccatcga caccatggtg gccctgatcc gcagcgtgct gcagatgcgg aactccaatg
     7321 ccgccacagg cgaggactat atcaacagcc ccgtgcgcga tctgaatggc gtgtgcttcg
     7381 actcccggtt tcagaaccca gagtggccca tggacgccga tgccaatggc gcctaccaca
     7441 tcgccctgaa gggccagctg ctgctgaatc acctgaagga gagcaaggat ctgaagctgc
     7501 agaacggcat ctccaatcag gactggctgg cctacatcca ggagctgcgc aacaaaaggc
     7561 cggcggccac gaaaaaggcc ggccaggcaa aaaagaaaaa gggatcctac ccatacgatg
     7621 ttccagatta cgcttatccc tacgacgtgc ctgattatgc atacccatat gatgtccccg
     7681 actatgcccc ggcagctaag aagaagaaac tggatggcag ccccgccgcc aagaagaaga
     7741 agctggacgg cagcaaacgt accgccgacg gcagcgagtt cgagagcccg aaaaagaaac
     7801 gcaaagtggg ttcggagaat ttgtattttc agggtctcga gcaccaccac caccaccact
     7861 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     7921 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     7981 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     8041 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     8101 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8161 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8221 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8281 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8341 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     8401 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     8461 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8521 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8581 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8641 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8701 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8761 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8821 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8881 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8941 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     9001 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     9061 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     9121 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     9181 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9241 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9301 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9361 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9421 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9481 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9541 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9601 cccgtagaaa agatcaaagg atcttc
//
