LOCUS       Exported               13677 bp ds-DNA     circular SYN 14-NOV-2023
DEFINITION  reference.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 28, 2024 from SnapGene Viewer 4.1.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13677
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    462..596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    596..620
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3100..3120)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(4562..4586)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8072..8092)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(8112..8128)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9209..9313
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9314..10174
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10345..10933
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    11221..11242
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11257..11287
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11295..11311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11319..11335
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11356..11374
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     11411..11427
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    12099..12233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     12249..12265
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12273..12293
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     polyA_signal    12294..12519
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(12887..13606)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     regulatory      13603..13612
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
ORIGIN
        1 cttctgatac aacacagcat ggtggcggcc ctagggttgc ctcagtgtgt agagatgttc
       61 aggatgctcc tttgccaagg tctgatgtct gtgagatgat ctgtgcgagc tttatattca
      121 cccgagtagg tgggtctaca atgggattat ggttagggtc ttagttttca gggactaatg
      181 tgtttaacta aaaggagtgc acaatctgtt aaatccaaaa gtttacagag tcaagggaag
      241 tgcctactct atgtttgttt gactaatcta tccatcaagt gaatagactt tttattttgt
      301 tttttctaca ttgaaaattc taaatcgatt tgcatcaacc ctatctttac ttcagctcta
      361 tagatgcatc agtggctcta gctatgcctg gagtggttga aaaaaacctc ccacacctcc
      421 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
      481 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      541 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcccac
      601 tttgtacaag aaagctgggt tggctttagt cgacggtatc gatagacgtc actattgctc
      661 atacgtgttg atatcgctaa aattacaccc tgggacaaac ccgaggttcg tcatgaactg
      721 cggatcaacg tcaaccgcat ccgtgtactc gatcgatgcc agatcatcca ccatcgcatc
      781 atagtctaca gtcgaatatc cgaaattggc catggggacg aggttactat ggtaaggagg
      841 atggttcgtt gtactatcaa cgtatggcgg gggtggtctg ggaggagcac ctccaggcgc
      901 aagggccacc gaggcctcgt tcaagtgggt ggcaggtggt ggatatgtac tgagaatgcc
      961 atgggtattg actgatccgt agtcattggt ttgagtaatt gcgtctaggg ccgcatcgct
     1021 attgtagggg gtaggattcg gtattccatt aaaccgatca ttgaccggat ttattgacgt
     1081 tgacaggggc tgttccatga catggggcat gatacttgca gtggcagtcg cagtcggcgg
     1141 aagctcaagt tgtcgtatgg gttggaggcc agtattgctg tgtcggcctc cccgaggcca
     1201 aatggaggag taggcgggga aaagctcttc caagtgcctt cgatgccgct ctgaagaatg
     1261 tgattgtttc tttaaagcaa gaagggagtc tagaagagaa gggatcagag gtgggattgc
     1321 atatgtacca aaccgcttct gatactgatc aagagtttcg atcagaagat gcgagtagtg
     1381 ttcgttgaga ggctcagaaa aggcagctgc ccataaataa acggtacggg taaggtatgc
     1441 agtaggcaca aaagccgatg ctgtatcctg ggaaaggaca aagacgctga agggagaatt
     1501 gtggccgatt acctgctgca accgcgacct gaactccaag ggagcgttct gtgaagatgt
     1561 tcgtattctt ggcagaagct ccaaagcaac cagcttggtc acgccatata ggtggttgaa
     1621 tgtgcttaaa gggtatgccg ggttcctcaa catttgcagc atggtgtgtt caccgcctaa
     1681 gccctcacat ggtgtccacg gctcccattg atcttgaccg tcttcaggta acggcagatc
     1741 cgcaatgtcg tctggcttga gatgggccgg cacgttgtgt ctcatagaca caatggtatc
     1801 cacgatgaag cacgcgacga gcagcgcacg cattcttggt cgtggccggt catcatcagg
     1861 aggcaactgg gtgcgaacaa caagtaggac gcggaccgca aagccaacca gcaaccaagc
     1921 agactcccaa tcatcccgcc caaggcttac gagacaaaga agcagcaacg ctctgcagtg
     1981 ctgaacctgg aacggcccgc tttccaaagg tatgagcttt cgggctgtgt cagatatgtc
     2041 tgcaggtgaa ggattgatgg cattgtcatg gccatgagca gctgaagctg gacccgtagc
     2101 actcgatgca gcagtagcag cagcctggaa ggacgccagg gcgagcgcgc tccagagctc
     2161 ggcatgaacg ccagactcga cattcgcaga gggaagcaac gagccttgtt cagagtactg
     2221 gtacaatgct tggtacatgt ctttcttctc gaggataggg agccaagaat gcgtgtagga
     2281 aaagtagata tccagcaggc gccaatgatt gggtggtagc ttccctatcc cggtaccatc
     2341 tggctgaagg ttgttttcca gggtgtttgc cgaaataggt ggtcggaccg ggcctggtag
     2401 agtgcgtgtc tcagctgtac tagacttagg cgccgagaaa gtcaagtgag gcgcatgagg
     2461 gaagtcggat gtctttgccc cggcatcttg gacatttatg tcttcggacg gggattggcc
     2521 gtcttcagat ggatcttgtg cggcctgacc tgagagaaga cgggtgatgc ttttgttgac
     2581 tcgactcgtc gcccatcttg catgcaggcg ttctgcagca ggcgagtctt tgccgaccag
     2641 gagagcgctg ccctggccag catcacggac caaaagattg tggagggcgt cctcgctgcg
     2701 ggcaacgttc tcgaacatcc aagccagagc cagttcgaga gtgcggatgt agcccgtctg
     2761 gactcctcgc ttcttgggac tggcctggta ggtacacgac cggccctgcg acacacaggg
     2821 gaagcaagcc ggctggattc catcacactt ttcacgtgca gctcgacact gatcacaggc
     2881 cctcgagact ctctggcgtt tcgaacgagc gggctcgaca agtaacgtcg tcgtggtagg
     2941 gccgaggccg ggtgtgtcaa tgttggtcaa gtccgcaaaa cgggcgttgt tgctgcttgc
     3001 tgcagtgtta gcgacatgac catcagcatt gccatcagca tggccatcag cgtgggcatt
     3061 ggcttcggca gcggcattga gtgtcttgcg tttaggcggg ctctggaagt acaggttctc
     3121 ggatccgaaa ggagactcag ccagtcttgg caggcctcca gccagagggg tctcagcgct
     3181 agagtcctga gatcctcctc ctcttccaaa ctggagttcc ttgctaggag gtccgtgtcc
     3241 aggagaagaa gaagctctgt ggttgctagt atttctctcc tcccacagca cctttcccag
     3301 tctccatctg tgccatcttc ttctgagctc agactgcacc tccttgttca gaaagcagta
     3361 cagcacagcc accagcagtc cccggaaaga agacaggaac aggtcaaaga acagcttggc
     3421 agagcgcagg gttccccggg catgctcatc agtgacaaaa gcaaacacca cctcatgcac
     3481 tcccagcaga gggatcaggg tcagggtgct cttggccagt ctgaacttgt agtcagtgtg
     3541 atgcatctgt ctggctctca gcttggccac cagcagctgc acaattctca caaagatgaa
     3601 gaagttgatc aggatggccg ggaacacagg gaatctgaga atccaccaga atcccatgtt
     3661 gtcattgcta gtccaacact gcacattctc aaacaggcac ttcaccacag cccaagggac
     3721 cacaaacagc attggagcgc cccatccaat tcccggtaca gagagaagaa agagcgctca
     3781 ggcagggtgg ccagtcccag cagattgtgc aggtacagtc cctccaccag cagccagcag
     3841 tagttggcca caattccata ttgcatgaac acagcagcca ctctgcagcc agccacagct
     3901 ccatcagaca gccggtgctc acagacaggt catctccaat cttctgagag tatctggttc
     3961 tgagcagtcc atcaatcacc agcacagaag aagccttcag cacaaaagaa gcaaacaggt
     4021 tggcatggat ggcatttctg gtacaatgga gcttgctcag tcctcccggg atggccagag
     4081 ccagcagcag agcgcccaga gacagagagt atcccacagt gtacatcacc cggaaagaag
     4141 agtacatctt ggccacctcc ttctgcacct caatctcctc tccatccatc tggcactgag
     4201 aagcatctct ccaaggctgt cctcttgggc ctctcaccca ctgtccatca ggtccgcatc
     4261 tcttgaacac aaatctgtgc tgcaccttgt ggtgccaagg cagataccaa gggcaagaga
     4321 tgttggcggt agtattggca ggggtgtcag gccagcaaga gtacttgtca aaggttctgt
     4381 tgcacaccag ctcagttgga ggaggcagca gagacaggtt gtggtggcac tggtctccat
     4441 acagcttcca cttctcaaac aggaagtcca tcacctgagc agaagggacc tgaggctgac
     4501 aagccagcag cagcagcagc agcagcagag gtctctgagg ctgacaaggt ggcatggtgg
     4561 cagcctgctt ttttgtacaa acttgaagct tgattgggtt gagtcttaca gtggtgtaat
     4621 cagtggatgg tgtggccctt tcttgttgct ttacttgccc tttgtgcaac accccgatgg
     4681 tttccagccg gctaagaatc tgggctgtca gaggctcctt ctgctgcctt ttatacactt
     4741 gtgacgcctt ggggagggtg ctgctctctc ctattcactt gctgtggtcc tgggctcaga
     4801 tgatgtaatg gtgggtggag ccactggccc tgagcattgt agggatggga accaatcaaa
     4861 tgacaaggga gggaatggaa atggatgctg cattgtctca ccatcaggca gataccacca
     4921 agaaggtgaa aaagatgctg ggaatgtgct tattgcttat tacaaaggaa tattatactg
     4981 aaataatgaa aaaaaattct aattaaaaca cgaatgagaa gctgattaag cctatagaaa
     5041 cattaacctt gttattgata atattagcct aatgatgact ccaaaggtat tagtgacagg
     5101 taccatcccc cacacaactc gtccatcact gggagctgta gggggaagga aggtgcagtg
     5161 gggatgggtg ccaccctatg cagctgcctc acaatttcat tttatcacat gatactgctc
     5221 catatccatt tctgcctcaa atcactctcc gggggattcc cgagtcagtg tttcaatatc
     5281 ccccctgtat tcaaatggaa gcagcacatc tgcctcagac cgcaaacaca caccagtttt
     5341 acagggcagg aaatcatatg gcccttaatt tatttaattg cttccgtaaa gaccagtgga
     5401 tgtagcagaa gaacgaggag gttgaacaaa cgggggcagt taaatggttt tactgacatc
     5461 tgtccaaaca tacagaacaa aaatgtgaaa taaaatcaca atcacaattt acatatgagt
     5521 agacagggct gaaaccttgc ctattaaaag tggcaaagct aggggaaacc agtgctattg
     5581 gattaacctt taccccggaa cctcccatcc aaatgtacat taaggttatg cccaaactag
     5641 aggcccatat gctttacata gtccatcttc tttctagtta cacccacata gaaatacttg
     5701 taagtgcccg ttggtagaag ctaagcatca gtaaatacat ctgcagtgga acacaagggg
     5761 gatcccggga gtaatcagct gatagctaga aaatgcagca aaatgtgtaa aatgctgctg
     5821 agcattagaa tcagcatgac agccggggtt ccattctttc tgtagcactg atgtgctgat
     5881 aaaagctgcc agagcagcat agtgattgca aatgagaaat attaatagcc aataaatcag
     5941 tgtaatttcc ttatcatgtc attacaatat atcacattta tgccactttt gtttggattg
     6001 tgtattattt ctatagagag aaaacacaac caatattaaa tattacaaat atttttatca
     6061 tactcacata tactgtacct aataagctgg cagacatcat atgaatttag gggtttggag
     6121 aaaaatgatt gaatcagtac atccttatgg gtggcaatac atggccaagt ataactgctg
     6181 actcagctct tgcagacata atagtgttta aaattgtgtc atactcacat atacctaata
     6241 agcacgcaga ctcggtacat ccttataggt ggtgattgat aaatggccaa gtataagctg
     6301 ccaactcagc tcttccagaa ttagttggca gttatggtta ccaccttttc tgggaaaaaa
     6361 gtaacagcct tcttatattt taatgttttt ttccaattat tgacattggc atcaagcaac
     6421 attttttctg gccgggctag taaaatatcc acaagtaggc aacccaatca gcagcttatt
     6481 ggcccatgca tgaggccttc tcaatggcct gcctgaccaa tatttgacta tcaagcagat
     6541 atatctatca gacagtttgg ataatcatgt tggaagagga ccacattggc aaatggctcc
     6601 caaatatgat ctgattgtta gcctgttgat cagatcatcc tgatttggcc cagtgtgttg
     6661 atgttgaata ttgtttggct atgcccacct taaggaaaat gtttgcattt caagattact
     6721 atgaaggagc attaaatgat taaaaaatac agaaagcaaa gctgatatca ctgaaagtgg
     6781 gcaacaccca agatttacaa tatattctcc tggtattaca ctatgagcat acaggagtct
     6841 gttccttact gcaatgttct gaaggtaatg taatcactgt tgttaatgtt aaaatgttag
     6901 taacagatgt actaccaata aatatggatt tcagtggtac agctgtgtgg gaagaggtcc
     6961 tcgagacaga cattcttact gtcagtcagg cagccttttg cagatatgca gacacagctt
     7021 tctcccttct tccttgtgct gcataagggg aggggtaggg cagagcagaa ttcacacaaa
     7081 gcatgaggag atgctaatga aagtgtcaca tggcatgggg gggagaagat gctgcatttg
     7141 gaagaggaga ctcacacacc gggaacatgg acattactct gtgtggagtg aagggaacac
     7201 attgttttga aaggatgaga aaagcaggac acggcaaagc tcataaaata gtctgtgctc
     7261 agtaaccaag caaactcatg acagcaaatg gacaccatta accgggttct cacatcatac
     7321 ctacatgtgt ataagtccat aaaagccatg cttctaaaat atcaaattat attctgtctt
     7381 gtcaagacac catattgctt atccatggca tatatcgaat tatttattct gcaggcagat
     7441 attatcctta aaataattat tcagtttaaa aataaaaact gggtaaatac tgtagattgg
     7501 ctatgcaaaa taaaaaatgt ttctaatata gttagttagc caaaaatgta atgtataaag
     7561 actggagtga cataagtaga acactacttc ctgctttgca ggtctcttga tttccactga
     7621 ttggttacaa ggttacaagg caataatcag agacttgagg ggggctgtaa ggcagggtgt
     7681 cccaaaatca gaacagacac caaggaccca gtcgcggctc acccttctgc ctataacagc
     7741 cgcctttggc atcgggacta gccctctgct actcagatga cgccaggtct taacgtgaga
     7801 ggcccaagga gagagttctg accaagcaag ggaaccaagt gtggggttgt agaattggaa
     7861 acaagaattg tagttaaaag ccaggccggg tcagtaacaa tcagtcaatg cggtacagtt
     7921 acaggagaca aataaatggt tggggtcaca ggccgagtca aacacaccaa aatagaaagt
     7981 acaccacagg atacaagaga gtagtcaaaa aacaggcaat aatccggaca ggcagcaaac
     8041 tagatgtaat caggaacaga cagggtctag acaacttttc tatacaaagt tgatagcttg
     8101 gcgtaatcat ggtcatagct gtttcctgct cgagggccca tctggcctgt gtttcagaca
     8161 ccgagggagt ctctgctcac gtttcctgct atttgcagcc tctctatcaa gactaataca
     8221 cctcttcccg catcggctgc ctgtgagagg cttttcagca ctgcaggatt gcttttcagc
     8281 cccaaaagag ctaggcttga cactaacaat tttgagaatc agcttctact gaagttaaat
     8341 ctgaggtttt acaactttga gtagcgtgta ctggcattag attgtctgtc ttatagtttg
     8401 ataattaaat acaaacagtt ctaaagcagg ataaaacctt gtatgcattt catttaatgt
     8461 tttttgagat taaaagctta aacaagaatc tctagttttc tttcttgctt ttacttttac
     8521 ttccttaata ctcaagtaca attttaatgg agtacttttt tacttttact caagtaagat
     8581 tctagccaga tacttttact tttaattgag taaaattttc cctaagtact tgtactttca
     8641 cttgagtaaa atttttgagt actttttaca cctctgtcaa gaactcctgg acaaacctct
     8701 gacctgtgtg gaacagagtg gatatgggtg tctgaacaga tattcacgtc ttttgcagat
     8761 cagagggcat ttctggtgtt gtgcaatgac ctgggtccaa caggtaggcg tactcgtatc
     8821 caagagcatt gttggacagg aggtagtctc cattcctata aagaggaagg aatggcacca
     8881 tgtagtagcc gtcattgtgg ccaattggtg cattggtacg tgggtagatg gaccggggag
     8941 gctgatgagt tcttagccag cgtgggccat cgccctgata gacggttttt cgccctttga
     9001 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     9061 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     9121 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     9181 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     9241 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     9301 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     9361 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     9421 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     9481 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     9541 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     9601 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     9661 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9721 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     9781 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     9841 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     9901 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     9961 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    10021 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    10081 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    10141 tgagataggt gcctcactta ttaagcattg gtaactgtca gaccaagttt actcatatat
    10201 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    10261 tgataatctc atgaccaaaa tgccttaacg tgagttttcg ttccactgag cgtcagaccc
    10321 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    10381 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    10441 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
    10501 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    10561 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    10621 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    10681 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    10741 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    10801 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10861 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10921 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    10981 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    11041 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    11101 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    11161 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    11221 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
    11281 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
    11341 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tccaccgcgg
    11401 tggcggccgc tctagaacta gtggatctgc tgggcttgct gaaggtaggg ggtcaagaac
    11461 cagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta ttatttttgg
    11521 ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta cttaattaca
    11581 tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt caaaaagtac
    11641 ttattttttg gagatcactt cattctattt tcccttgcta ttaccaaacc aattgaattg
    11701 cgctgatgcc cagtttaatt taaatgttat ttattctgcc tatgaaaatc gttttcacat
    11761 tatatgaaat tggtcagaca tgttcattgg tcctttggaa gtgacgtcat gtcacatcta
    11821 ttaccacaat gcacagcacc ttgacctgga aattagggaa attataacag tcaatcagtg
    11881 gaagaaaatg gaggaagtat gtgattcatc agcagctgcg agcagcacag tccaaaatca
    11941 gccacaggat caagagcacc cgtggccgta tcttcgcaga tctgatctag aggatcataa
    12001 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
    12061 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
    12121 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    12181 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcatcatcg
    12241 atggtaccgt aaaacgacgg ccagtgaatt atcaactatg tataataaag ttgccataga
    12301 gcccaccgca tccccagcat gccttgctat tgtcttccca atcctccccc ttgctgtcct
    12361 gccccacccc accccccaga atagaatgac acctactcag acaatgcgat gcaatttcct
    12421 cattttatta ggaaaggaca gtgggagtgg caccttccgg ggtcaaggaa ggcacggggg
    12481 aggggcaaac aacagatggc tggcaactag aaggcacagt cgaggctgat cagcgggttt
    12541 cgggcccccc ctcgtgccct acaggttgtc ttcccaactt gccccttgct ccataccacc
    12601 cccctccacc ccataatatt atagaaggac acctagtcag acaaaatgat gcaacttaat
    12661 tttattagga caaggctggt gggcactgga gtggcaactt ccgggccagg agaggcactg
    12721 gggaggggtc acagggatgc cacccaattc ctgcagaatt cgataacaac aacaattgca
    12781 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
    12841 tctacaaatg tggtatggct gattatgatc tagagtcgcg gccgctttac ttgtacagct
    12901 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
    12961 gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca
    13021 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
    13081 tgccgtcctc gatgttgtgg cggatcttga agttggcctt gatgccgttc ttctgcttgt
    13141 cggcggtgat atagacgttg tggctgatgt agttgtactc cagcttgtgc cccaggatgt
    13201 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
    13261 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
    13321 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg
    13381 ggtagcggct gaagcactgc acgccccagg tcagggtggt cacgagggtg ggccagggca
    13441 cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc
    13501 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
    13561 ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggtg gcggccggtg
    13621 gatcccggcc cgcggtaccg tgctctgcag ttcctggtca gcctcctcat tcttctc
//
