LOCUS       Exported                7010 bp ds-DNA     circular SYN 28-MAR-2024
DEFINITION  4_1571_pExpTol2-UAS_sGCG-ICAM(1235)-Nrxn1b_cryaa-mCherry.ape (7010 
            bp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 28, 2024 from SnapGene Viewer 4.1.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    708..732
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        736..754
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     780..796
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1334..1363
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    2747..2881
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      3632..3641
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3638..4348
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGHHSTGGMDELYK"
     polyA_signal    4358..4479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5267..5855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6026..6886)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6887..6991)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgtccaggag ttcttgacag aggtgtaaaa agtactcaaa aattttactc aagtgaaagt
       61 acaagtactt agggaaaatt ttactcaatt aaaagtaaaa gtatctggct agaatcttac
      121 ttgagtaaaa gtaaaaaagt actccattaa aattgtactt gagtattaag gaagtaaaag
      181 taaaagcaag aaagaaaact agagattctt gtttaagctt ttaatctcaa aaaacattaa
      241 atgaaatgca tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta
      301 taagacagac aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac
      361 ttcagtagaa gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa
      421 agcaatcctg cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta
      481 gtcttgatag agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa
      541 acacaggcca gatgggccat tcgtacgctt aatttcgaat atcgatatca agggcccatc
      601 tcgagtatcc ggacacctag gtagcatgca tggcgccttg tcgactagct agcatcccgg
      661 gaagaattcc tcgacggatc caccggtgtg gaattctgca gatatcaaca agtttgtaca
      721 aaaaagcagg cttagtaata cgactcacta tagggcgaat tgggtaccgg gccccccctc
      781 gaggtcgacg gtatcgataa gcttaggcct ccaaggcgga gtactgtcct ccgggctggc
      841 ggagtactgt cctccggcaa ggtcggagta ctgtcctccg acactagagg tcggagtact
      901 gtcctccgac gcaaggcgga gtactgtcct ccgggctgcg gagtactgtc ctccggcaag
      961 gtcggagtac tgtcctccga cactagaggt cggagtactg tcctccgacg caaggcggag
     1021 tactgtcctc cgggctggcg gagtactgtc ctccggcaag ggtcgactct agagggtata
     1081 taatggatcc catcgcgtct cagcctcact ttgagctcct ccacacgaat tcctgcagcc
     1141 cgggggatcc gcggccgcca ccatgagtgc acttctgatc ctagcccttg tgggagctgc
     1201 tgttgctcat tcacagggca cattcaccag tgactacagc aagtatctgg actccaagaa
     1261 ggcccaagag tttgtgcagt ggttgatgaa tacaggtacc ggcaacggaa atgggaacgg
     1321 taatggcacc ggtgagcaaa agctcatttc tgaagaggac ttggggaacg gaaatggcaa
     1381 tggtaacggg aatggaaacg gcaatggtaa cgggaatact agtcagacat ctgtgtcccc
     1441 ctcaaaagtc atcctgcccc ggggaggctc cgtgctggtg acatgcagca cctcctgtga
     1501 ccagcccaag ttgttgggca tagagacccc gttgcctaaa aaggagttgc tcctgcctgg
     1561 gaacaaccgg aaggtgtatg aactgagcaa tgtgcaagaa gatagccaac caatgtgcta
     1621 ttcaaactgc cctgatgggc agtcaacagc taaaaccttc ctcaccgtgt actggactcc
     1681 agaacgggtg gaactggcac ccctcccctc ttggcagcca gtgggcaaga accttaccct
     1741 acgctgccag gtggagggtg gggcaccccg ggccaacctc accgtggtgc tgctccgtgg
     1801 ggagaaggag ctgaaacggg agccagctgt gggggagccc gctgaggtca cgaccacggt
     1861 gctggtgagg agagatcacc atggagccaa tttctcgtgc cgcactgaac tggacctgcg
     1921 gccccaaggg ctggagctgt ttgagaacac ctcggccccc taccagctcc agacctttgt
     1981 cctgccagcg actcccccac aacttgtcag cccccgggtc ctagaggtgg acacgcaggg
     2041 gaccgtggtc tgttccctgg acgggctgtt cccagtctcg gaggcccagg tccacctggc
     2101 actgggggac cagaggttga accccacagt cacctatggc aacgactcct tctcggccaa
     2161 ggcctcagtc agtgtgaccg cagaggacga gggcacccag cggctgacgt gtgcagtaat
     2221 actggggaac cagagccagg agacactgca gacagtgacc atctacagct ttccgtccca
     2281 gcagactcca atgtgccagg cttgggggaa cccattgccc gagctcaagt gtctaaagga
     2341 tggcactttc ccactgccca tcggggaatc agtgactgtc actcgagatc ttgagggcac
     2401 ctacctctgt cgggccagga gcactcaagg ggaggtcacc cgcgaggtga ccgtgaatgt
     2461 gctctccccc cggtatgagg ggatggtggt gggcatcgtg gcgggcgcgg cgctctgcat
     2521 cctcatcctg ctctacgcca tgtacaaata ccgcaaccga gacgaggggt cgtaccatgt
     2581 ggacgagagc agaaactaca tctgcaactc caacggagcc gcgctgaagg agaaaaacac
     2641 tgcagacgac gacagcggga gcaagagcaa gaagaacaaa aacaaggagt attacgtgtg
     2701 attaattaag ccaccgcggt ggagctcgaa ttaattcatc gatgatgatc cagacatgat
     2761 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2821 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2881 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2941 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc tagatcagat
     3001 ctaatctgat cattcaggcg cgccatacaa tacatttcct acaataatgc tctcttttct
     3061 attaatagtg tgcattcagt gcaggatgtt atgtatttat tttctgtagt gcaaactttt
     3121 tcaaagaatt ttccagtcta agcttttcag tcaagaaaaa aaaaagcgat tgattatctg
     3181 tgatatttaa aaaaattagc tcttaaatct ggtgaaccaa gtggccacaa cacattaaat
     3241 actgacgttc tacagccatg tagcttcatc tggtctggtt tgttttggca ggcacttatt
     3301 ttccggtgat agttggccac aagagtattg tctgagccat tcagtgctag actgtcattc
     3361 tcaggtcaga gtccaccccg ctgattctgc tgacaacact gttcccacca tgagataatg
     3421 ccattccaga gagatccatt tgtaagcccc tctttctgca gcacaggtat ataaccaggg
     3481 gtctgcctcc actaaggccg gcacacatca tttggggatc tttgtactgt actagcctct
     3541 attagcctcc tacttgcgtt cagttcacat cttttgttca tcaggtgcaa taagttacag
     3601 taaggagtta ccaggtctga cattaccggt cgccaccatg gtgagcaagg gcgaggatga
     3661 caacatggcc atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa
     3721 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     3781 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
     3841 tcagttcatg tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     3901 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     3961 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     4021 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg
     4081 ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca
     4141 gaggctgaag ctgaaggacg gcggccacta cgacgccgag gtcaagacca cctacaaggc
     4201 caagaagccc gtgcagctgc ccggcgccta caacgtcaac atcaagctgg acatcacctc
     4261 ccacaacgag gactacacca tcgtggaaca gtacgagcgc gccgagggcc accactccac
     4321 cggcggcatg gacgagctgt acaagtaaca gacatgataa gatacattga tgagtttgga
     4381 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     4441 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     4501 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt gtttaaacga tctgcgaaga
     4561 tacggccacg ggtgctcttg atcctgtggc tgattttgga ctgtgctgct cgcagctgct
     4621 gatgaatcac atacttcctc cattttcttc cactgattga ctgttataat ttccctaatt
     4681 tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac ttccaaagga
     4741 ccaatgaaca tgtctgacca atttcatata atgtgaaaac gattttcata ggcagaataa
     4801 ataacattta aattaaactg ggcatcagcg caattcaatt ggtttggtaa tagcaaggga
     4861 aaatagaatg aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag
     4921 gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt
     4981 tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa
     5041 gtaaaattac tcaagtactt tacacctctg gttcttgagg tacctcttcc gcttcctcgc
     5101 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5161 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5221 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5281 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5341 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5401 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5461 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5521 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5581 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5641 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5701 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     5761 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     5821 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     5881 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     5941 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6001 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6061 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6121 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6181 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6241 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6301 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6361 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6421 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6481 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6541 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6601 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6661 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6721 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     6781 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     6841 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     6901 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaggtacc
//
