LOCUS       pGP-AAV-CAG-flex-iGABASnFR2(no_bind)-WPRE      6968 bp    DNA     circular UNA 
FEATURES             Location/Qualifiers
     misc_feature    46..212
                     /note="ITR"
     promoter        237..1171
                     /note="CAG promoter"
     misc_feature    1180..1213
                     /note="loxP2272"
     misc_feature    1276..1309
                     /note="loxP"
     CDS             complement(1320..3260)
                     /label="iGABASnFR2(no bind) (var 4444)"
     misc_feature    complement(3277..3310)
                     /note="loxP2272"
     misc_feature    complement(3381..3414)
                     /note="loxP"
     misc_feature    3456..4044
                     /note="WPRE"
     misc_feature    4072..4304
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(4332..4498)
                     /label="ITR"
     Origin          complement(4736..5324)
                     /label="ori"
     gene            complement(5498..6355)
                     /note="Amp"
     Origin          complement(6486..6941)
                     /label="f1-ori"
ORIGIN
        1 GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG CCAGCTGCGC GCTCGCTCGC
       61 TCACTGAGGC CGCCCGGGCA AAGCCCGGGC GTCGGGCGAC CTTTGGTCGC CCGGCCTCAG
      121 TGAGCGAGCG AGCGCGCAGA GAGGGAGTGG CCAACTCCAT CACTAGGGGT TCCTTGTAGT
      181 TAATGATTAA CCCGCCATGC TACTTATCTA CGTAGCCATG CTCTAGGAAG AGTACCATTG
      241 ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA
      301 TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA
      361 AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC
      421 ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC
      481 ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA TCTCCCCCCC CTCCCCACCC
      541 CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG CGATGGGGGC GGGGGGGGGG
      601 GGGGGGCGCG CGCCAGGCGG GGCGGGGCGG GGCGAGGGGC GGGGCGGGGC GAGGCGGAGA
      661 GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT TTCCTTTTAT GGCGAGGCGG
      721 CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG CGGGAGTCGC TGCGACGCTG
      781 CCTTCGCCCC GTGCCCCGCT CCGCCGCCGC CTCGCGCCGC CCGCCCCGGC TCTGACTGAC
      841 CGCGTTACTC CCACAGGTGA GCGGGCGGGA CGGCCCTTCT CCTCCGGGCT GTAATTAGCG
      901 CTTGGTTTAA TGACGGCTTG TTTCTTTTCT GTGGCTGCGT GAAAGCCTTG AGGGGCTCCG
      961 GGAGGGCCCT TTGTGCGGGG GGAGCGGCTC GGGGCTGTCC GCGGGGGGAC GGCTGCCTTC
     1021 GGGGGGGACG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT GACCGGCGGC TCTAGAGCCT
     1081 CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT CCTGGGCAAC GTGCTGGTTA
     1141 TTGTGCTGTC TCATCATTTT GGCAAAGAAT TGGATCCCCA TAACTTCGTA TAAAGTATCC
     1201 TATACGAAGT TATATCAAAA TAGGAAGACC AATGCTTCAC CATCGACCCG AATTGCCAAG
     1261 CATCACCATC GACCCATAAC TTCGTATAAT GTATGCTATA CGAAGTTATA CTAGAGCTTT
     1321 CAACGTGGCT TCTTCTGCCA AAGCATGATG AGGATGATAA GGGAGATGAT GGTGAGCACC
     1381 ACCAGGGCCA GGATGGCTGA GATCACCACC ACCTTAAAGG GCAAGGAGTG TGGCACCACG
     1441 ATGACCTCCT GCGTGTCCTG GCCCACAGCA TTCAGATCCT CTTCTGAGAT GAGTTTTTGT
     1501 TCGTCGACCT GCAGTTTGAC TAGCCATTCA TTCCACCGTG TCGCAATGGC CGGACCGTTC
     1561 TTGGCCCAGT AGGCGAAATC GAGGGTGATC TGATCCTTGA CGTAGGCGGT CGGCAGGTTC
     1621 GGTGCCAGGT TGGCGTTCCA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC
     1681 TTGAAGTCGA TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC
     1741 TCGGCGCGGG TCTTGTAGGT GCCGTCGTCC TTGAAGCTGA TGGTGCGCTC CTGGACGTAG
     1801 CCTTCGGGCA TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGCTG
     1861 AAGCACTGCA CGCCGTAGGT CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG
     1921 CCGGTGGTGC AGATGAACTT CAGGGTCAGC TTGCCGTTGG TGGCATCGCC CTCGCCCTCG
     1981 CCGCGCACGC TGAACTTGTG GCCGTTTACG TCGCCGTCCA GCTCGACCAG GATGGGCACC
     2041 ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC ATGCTCCCTC CGGTACCGCC CTTGTACAGC
     2101 TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT CCAGCAGGAC CATGTGATCG
     2161 CGCTTCTCGT TGGGGTCTTT GCTCAGCACG GACTGGGTGC TCAGGTAGTG GTTGTCGGGC
     2221 AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG
     2281 CTGCCGTCCT CCACGTTGTG CGGGATCTTG AAGTTCGCCA TGATGCCGTT CTTCTGCTTG
     2341 TCGGCGGTGA TCCGGACCTG CGCAAGGTCG AGGCGCTGCA CACTGTCGAT GTTCACTGGA
     2401 GCATAGGCGG TCAGGGCGGA AAAGTCGTCC TGGCCTTTGG CGCTGCTGGC ACTGGCCAGG
     2461 AACTTCATCG CGGCGGCTTT GTTCTTCGTG CCTTTAGGCA CGACGAGGAT GTCGGCCATC
     2521 ACCAGGTTCT GCTTCCAGCT CACGCCCACT GGCGCGCCGT CTTCTTGCAG GGCGTGGATG
     2581 CGACCGTTCC AGAACTGGCC CATGCTGACT TCGCCGGAGG CCAGCAGCTG CTGCGACTGT
     2641 GCACCGCCGC CCCACCAGAC GATGTCTTTC TTGATGGTGT CGAGTTTCTT GAAGGCGCGG
     2701 TCCAGGTCCA GCGGGTAGAG CTTGTCGGCC GGTACGCCGT CGGCGAGCAG GGCCAGTTCG
     2761 AGCACGCCAG GGCTCGGCCA TTTGTAGAGG GCGCGTTTGC CGGGGTAGGT CTTGGTGTCG
     2821 AACAGCGCGG TCCAGTCCTG GGGCTTGCTG GCTCCGAGCT TGCCTTCGTT GTAGCCGAGC
     2881 ACGAAAGACC CCAGGAAGGC CCCCACTCCA TGGTCACTCA CGAACCGCGG ATCGATCTTG
     2941 TCGCGCTGGA TCACCGAGAA ATCCAGGGGT TCGAGCAGGC CTTCGGCGGC GGCGCGCAAG
     3001 GCGAAGTCGG CCTCTACATC CACCACGTCC CACTGCACGT TGCCGCTTTC GACCATGGCC
     3061 TTGAGTTTGC CGTAGTCGGT GGGCCCATCC TGGACCACGG TAATGCCGCT GGCCTTGCTG
     3121 AACGGGTCGG CCCAGGCCTG CTTCTGCGCA TCCTGGGTGC TACCGCCCCA GCTCACGAAA
     3181 TTGATGCTTT CAGATCTGTC ACCAGTGGAA CCTGGAACCC AGAGCAGCAG TACCCATAGC
     3241 AGGAGTGTGT CTGTCTCCAT GGTGGCGGAT CCTAGTATAA CTTCGTATAG GATACTTTAT
     3301 ACGAAGTTAT CATTGGGATT CTTCCTATTT TGATCCAAGC ATCACCATCG ACCCTCTAGT
     3361 CCAGATCTCA CCATCGACCC ATAACTTCGT ATAGCATACA TTATACGAAG TTATGTCCCT
     3421 CGAAGAGGTT CGAATTCGAT ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT
     3481 GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG
     3541 CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT
     3601 ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG
     3661 TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC
     3721 AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG
     3781 CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT
     3841 TGTCGGGGAA ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC
     3901 GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG
     3961 GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA
     4021 TCTCCCTTTG GGCCGCCTCC CCGCATCGAT ACCGTCGACC CGGGCGGCCG CTTCGAGCAG
     4081 ACATGATAAG ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT
     4141 GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA
     4201 AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGATGTGGG
     4261 AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAA AATCGATAAG GATCTTCCTA
     4321 GAGCATGGCT ACGTAGATAA GTAGCATGGC GGGTTAATCA TTAACTACAA GGAACCCCTA
     4381 GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA
     4441 AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC
     4501 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG
     4561 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
     4621 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
     4681 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
     4741 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
     4801 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
     4861 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
     4921 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
     4981 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
     5041 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
     5101 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
     5161 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
     5221 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
     5281 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
     5341 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
     5401 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
     5461 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC
     5521 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
     5581 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC
     5641 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
     5701 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA
     5761 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG
     5821 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
     5881 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG
     5941 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
     6001 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT
     6061 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA
     6121 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
     6181 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC
     6241 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
     6301 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT
     6361 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
     6421 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
     6481 CACCTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG
     6541 CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC
     6601 CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA
     6661 CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC
     6721 ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG
     6781 GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA
     6841 GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC
     6901 CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCCCATTCGC CATTCAGGCT
     6961 GCGCAACT
//
