LOCUS       pEN-X-hPGK-BlastR-Z      3161 bp ds-DNA     circular SYN 15-NOV-2017
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct
            synthetic DNA construct
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Twistbioscience
  TITLE     Direct Submission
  JOURNAL   Exported Nov 15, 2017 from SnapGene 4.1.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     promoter        146..656
                     /label="hPGK promoter"
     mutation        246
                     /label="T246A"
     mutation        398
                     /label="T398C"
     CDS             665..1063
                     /label="BlastR"
     CDS             complement(2083..2898)
                     /label="Kanamycin"
ORIGIN
        1 AATAACTGTG ATCCACCACA AGCGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG
       61 GCCAGTCATG CATAATCCGC ACGCATCTGG AATAAGGAAG TGCCATTCCG CCTGACCTGA
      121 AGACGTGTGA CGTCTCCGCA AACCTGGGGT TGGGGTTGCG CCTTTTCCAA GGCAGCCCTG
      181 GGTTTGCGCA GGGACGCGGC TGCTCTGGGC GTGGTTCCGG GAAACGCAGC GGCGCCGACC
      241 CTGGGACTCG CACATTCTTC ACGTCCGTTC GCAGCGTCAC CCGGATCTTC GCCGCTACCC
      301 TTGTGGGCCC CCCGGCGACG CTTCCTGCTC CGCCCCTAAG TCGGGAAGGT TCCTTGCGGT
      361 TCGCGGCGTG CCGGACGTGA CAAACGGAAG CCGCACGCCT CACTAGTACC CTCGCAGACG
      421 GACAGCGCCA GGGAGCAATG GCAGCGCGCC GACCGCGATG GGCTGTGGCC AATAGCGGCT
      481 GCTCAGCAGG GCGCGCCGAG AGCAGCGGCC GGGAAGGGGC GGTGCGGGAG GCGGGGTGTG
      541 GGGCGGTAGT GTGGGCCCTG TTCCTGCCCG CGCGGTGTTC CGCATTCTGC AAGCCTCCGG
      601 AGCGCACGTC GGCAGTCGGC TCCCTCGTTG ACCGAATCAC CGACCTCTCT CCCCAGAACA
      661 GACCATGGCC AAGCCTTTGT CTCAAGAAGA ATCCACCCTC ATTGAAAGAG CAACGGCTAC
      721 AATCAACAGC ATCCCCATCT CTGAAGACTA CAGCGTCGCC AGCGCAGCTC TCTCTAGCGA
      781 CGGCCGCATC TTCACTGGTG TCAATGTATA TCATTTTACT GGGGGACCTT GTGCAGAACT
      841 CGTGGTGCTG GGCACTGCTG CTGCTGCGGC AGCTGGCAAC CTGACTTGTA TCGTCGCGAT
      901 CGGAAATGAG AACAGGGGCA TCTTGAGCCC CTGCGGACGG TGCCGACAGG TGCTTCTCGA
      961 TCTGCATCCT GGGATCAAAG CCATAGTGAA GGACAGTGAT GGACAGCCGA CGGCAGTTGG
     1021 GATTCGTGAA TTGCTGCCCT CTGGTTATGT GTGGGAGGGC TAAGGCTCTA AGGTTAAGTC
     1081 GCCCTCGGTA TCAGATGAGA CGCGTATAGT CTTCAGGCTA GGTGGAGGCT CAGTGATGAT
     1141 AAGTCTGCGA TGGTGGATGC ATGTGTCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA
     1201 TCCGCTCAGA GGGCACAATC CTATTCCGCG CTATCCGACA ATCTCCAAGA CATTAGGTGG
     1261 AGTTCAGTTC GGCGTATGGC ATATGTCGCT GGAAAGAACA TGTGAGCAAA AGGCCAGCAA
     1321 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT
     1381 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA
     1441 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG
     1501 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA
     1561 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA
     1621 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG
     1681 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG
     1741 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA
     1801 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC
     1861 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG
     1921 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     1981 GCTCTATTCA ACAAAGCCGC CGTCCCGTCA AGTCAGCGTA AATGGGTAGG GGGCTTCAAA
     2041 TCGTCCGCTC TGCCAGTGTT ACAACCAATT AACAAATTCT GATTAGAAAA ACTCATCGAG
     2101 CATCAAATGA AACTGCAATT TATTCATATC AGGATTATCA ATACCATATT TTTGAAAAAG
     2161 CCGTTTCTGT AATGAAGGAG AAAACTCACC GAGGCAGTTC CATAGGATGG CAAGATCCTG
     2221 GTATCGGTCT GCGATTCCGA CTCGTCCAAC ATCAATACAA CCTATTAATT TCCCCTCGTC
     2281 AAAAATAAGG TTATCAAGTG AGAAATCACC ATGAGTGACG ACTGAATCCG GTGAGAATGG
     2341 CAAAAGCTTA TGCATTTCTT TCCAGACTTG TTCAACAGGC CAGCCATTAC GCTCGTCATC
     2401 AAAATCACTC GCATCAACCA AACCGTTATT CATTCGTGAT TGCGCCTGAG CCAGACGAAA
     2461 TACGCGATCG CTGTTAAAAG GACAATTACA AACAGGAATC GAATGCAACC GGCGCAGGAA
     2521 CACTGCCAGC GCATCAACAA TATTTTCACC TGAATCAGGA TATTCTTCTA ATACCTGGAA
     2581 TGCTGTTTTC CCGGGGATCG CAGTGGTGAG TAACCATGCA TCATCAGGAG TACGGATAAA
     2641 ATGCTTGATG GTCGGAAGAG GCATAAATTC CGTCAGCCAG TTTAGTCTGA CCATCTCATC
     2701 TGTAACATCA TTGGCAACGC TACCTTTGCC ATGTTTCAGA AACAACTCTG GCGCATCGGG
     2761 CTTCCCATAC AATCGATAGA TTGTCGCACC TGATTGCCCG ACATTATCGC GAGCCCATTT
     2821 ATACCCATAT AAATCAGCAT CCATGTTGGA ATTTAATCGC GGCCTCGAGC AAGACGTTTC
     2881 CCGTTGAATA TGGCTCATAA CACCCCTTGT ATTACTGTTT ATGTAAGCAG ACAGTTTTAT
     2941 TGTTCATGAT GATATATTTT TATCTTGTGC AATGTAACAT CAGAGATTTT GAGACACAAC
     3001 GTGGCTTTCC CCCGCCGCTC TAGAACTAGT GGATCCAAAT AAAACGAAAG GCTCAGTCGA
     3061 AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGCATTA TAGCAGACGT CCAGGTTGGG
     3121 ATACCTGAAA CAAAACCCAT CGTACGGCCA AGGAAGTCTC C
//
