LOCUS       pEN-X-mPGK-NeoR-SV40_polyA-Z      3675 bp ds-DNA     circular SYN 15-NOV-2017
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct (unknown)
  ORGANISM  synthetic DNA construct
            synthetic DNA construct
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Twistbioscience
  TITLE     Direct Submission
  JOURNAL   Exported Nov 15, 2017 from SnapGene 4.1.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     promoter        147..657
                     /label="mPGK promotor"
     mutation        395
                     /label="T395C"
     CDS             667..1461
                     /label="NeoR"
     pA              1470..1589
                     /label="SV40 polyA"
     CDS             complement(2598..3413)
                     /label="Kanamycin"
ORIGIN
        1 CAATAACTGT GATCCACCAC AAGCGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC
       61 GGCCAGTCAT GCATAATCCG CACGCATCTG GAATAAGGAA GTGCCATTCC GCCTGACCTG
      121 AAGACGTGTG ACGTCTCCGC AAACCTTTCT ACCGGGTAGG GGAGGCGCTT TTCCCAAGGC
      181 AGTCTGGAGC ATGCGCTTTA GCAGCCCCGC TGGGCACTTG GCGCTACACA AGTGGCCTCT
      241 GGCCTCGCAC ACATTCCACA TCCACCGGTA GGCGCCAACC GGCTCCGTTC TTTGGTGGCC
      301 CCTTCGCGCC ACCTTCTACT CCTCCCCTAG TCAGGAAGTT CCCCCCCGCC CCGCAGCTCG
      361 CGTCGTGCAG GACGTGACAA ATGGAAGTAG CACGCCTCAC TAGTCTCGTG CAGATGGACA
      421 GCACCGCTGA GCAATGGAAG CGGGTAGGCC TTTGGGGCAG CGGCCAATAG CAGCTTTGCT
      481 CCTTCGCTTT CTGGGCTCAG AGGCTGGGAA GGGGTGGGTC CGGGGGCGGG CTCAGGGGCG
      541 GGCTCAGGGG CGGGGCGGGC GCCCGAAGGT CCTCCGGAGG CCCGGCATTC TGCACGCTTC
      601 AAAAGCGCAC GTCTGCCGCG CTGTTCTCCT CTTCCTCATC TCCGGGCCTT TCGACCTAAC
      661 ACCACCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
      721 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
      781 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
      841 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
      901 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
      961 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
     1021 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
     1081 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
     1141 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
     1201 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
     1261 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
     1321 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
     1381 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
     1441 CTTCTTGACG AGTTCTTCTG AGGCTCCACT TGTTTATTGC AGCTTATAAT GGTTACAAAT
     1501 AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG
     1561 GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGCT CTGTATCAGA TGAGACGCGT
     1621 ATAGTCTTCA GGCTAGGTGG AGGCTCAGTG ATGATAAGTC TGCGATGGTG GATGCATGTG
     1681 TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCAGAGGGCA CAATCCTATT
     1741 CCGCGCTATC CGACAATCTC CAAGACATTA GGTGGAGTTC AGTTCGGCGT ATGGCATATG
     1801 TCGCTGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG
     1861 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT
     1921 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA
     1981 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC
     2041 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT
     2101 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG
     2161 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG
     2221 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT
     2281 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC
     2341 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG
     2401 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC
     2461 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCT ATTCAACAAA GCCGCCGTCC
     2521 CGTCAAGTCA GCGTAAATGG GTAGGGGGCT TCAAATCGTC CGCTCTGCCA GTGTTACAAC
     2581 CAATTAACAA ATTCTGATTA GAAAAACTCA TCGAGCATCA AATGAAACTG CAATTTATTC
     2641 ATATCAGGAT TATCAATACC ATATTTTTGA AAAAGCCGTT TCTGTAATGA AGGAGAAAAC
     2701 TCACCGAGGC AGTTCCATAG GATGGCAAGA TCCTGGTATC GGTCTGCGAT TCCGACTCGT
     2761 CCAACATCAA TACAACCTAT TAATTTCCCC TCGTCAAAAA TAAGGTTATC AAGTGAGAAA
     2821 TCACCATGAG TGACGACTGA ATCCGGTGAG AATGGCAAAA GCTTATGCAT TTCTTTCCAG
     2881 ACTTGTTCAA CAGGCCAGCC ATTACGCTCG TCATCAAAAT CACTCGCATC AACCAAACCG
     2941 TTATTCATTC GTGATTGCGC CTGAGCCAGA CGAAATACGC GATCGCTGTT AAAAGGACAA
     3001 TTACAAACAG GAATCGAATG CAACCGGCGC AGGAACACTG CCAGCGCATC AACAATATTT
     3061 TCACCTGAAT CAGGATATTC TTCTAATACC TGGAATGCTG TTTTCCCGGG GATCGCAGTG
     3121 GTGAGTAACC ATGCATCATC AGGAGTACGG ATAAAATGCT TGATGGTCGG AAGAGGCATA
     3181 AATTCCGTCA GCCAGTTTAG TCTGACCATC TCATCTGTAA CATCATTGGC AACGCTACCT
     3241 TTGCCATGTT TCAGAAACAA CTCTGGCGCA TCGGGCTTCC CATACAATCG ATAGATTGTC
     3301 GCACCTGATT GCCCGACATT ATCGCGAGCC CATTTATACC CATATAAATC AGCATCCATG
     3361 TTGGAATTTA ATCGCGGCCT CGAGCAAGAC GTTTCCCGTT GAATATGGCT CATAACACCC
     3421 CTTGTATTAC TGTTTATGTA AGCAGACAGT TTTATTGTTC ATGATGATAT ATTTTTATCT
     3481 TGTGCAATGT AACATCAGAG ATTTTGAGAC ACAACGTGGC TTTCCCCCGC CGCTCTAGAA
     3541 CTAGTGGATC CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG
     3601 TTGTTTGTCG CATTATAGCA GACGTCCAGG TTGGGATACC TGAAACAAAA CCCATCGTAC
     3661 GGCCAAGGAA GTCTC
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