LOCUS       pCL376_GSDMA            7573 bp    DNA     circular SYN 30-MAY-2025
DEFINITION  pCL376_GSDMA.
ACCESSION   pCL376_GSDMA
VERSION     pCL376_GSDMA
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     polyA_signal    complement(3002..3624)
                     /note="pLannotate"
                     /label="hGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     rep_origin      complement(5983..6411)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(3641..4975)
                     /note="pLannotate"
                     /label="GSDMA"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(2663..2960)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(5058..5257)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1818..2609)
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    complement(1029..1163)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        6438..6542
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1417..1794)
                     /note="pLannotate"
                     /label="ble"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             6543..7403
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(5259..5638)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        1005..1023
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             5798..5952
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.1"
                     /fragment="True"
                     /other="CDS"
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4992..5012)
                     /note="pLannotate"
                     /label="3xFLAG (fragment)"
                     /database="snapgene"
                     /identity="95.2"
                     /match_length="29.2"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1186..1269)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
     rep_origin      1270..1293
                     /note="pLannotate"
                     /label="CloDF13 ori (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.2"
                     /fragment="True"
                     /other="rep_origin"
     CDS             complement(1316..1375)
                     /note="pLannotate"
                     /label="str (fragment)"
                     /database="swissprot"
                     /identity="95.0"
                     /match_length="7.5"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga catgattacg aatttaatac gactcactat
     1021 agggaattga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1081 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1141 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1201 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     1261 attatgatcg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttccagc
     1321 ttggcgagtg ggtggtgagc agctcgccgt cgcgcagcag gcgccagcgc tccatccacg
     1381 cagtgacctc aggcggaaaa cgggaagaca cactcatgag atgcctgcaa gcaattcgtt
     1441 ctgtatcagg cgcaggagcg tcccgtccgg gtcgaccaaa gcggccatcg tgcctcccca
     1501 ctcctgcagt tcgggggcat ggatgcgcgg atagccgctg ctggtttcct ggatgccgac
     1561 ggatttgcac tgccggtaga actccgcgag gtcgtccagc ctcaggcagc agctgaacca
     1621 actcgcgagg ggatcgagcc cggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1681 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1741 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1801 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1861 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1921 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1981 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     2041 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     2101 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     2161 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     2221 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     2281 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     2341 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     2401 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     2461 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2521 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2581 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2641 gatcccaagc tgatcaattc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     2701 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     2761 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     2821 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     2881 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     2941 gcctggggac tttccacacc ctaactgaca cacattccac agccaagctg gccgcgtacc
     3001 caattcaaca ggcatctact gagtggaccc aacgcatgag aggacagtgc caagcaagca
     3061 actcaaatgt cccaccggtt gggcatggcc aggtagccta tgctgtgtct ggacgtcctc
     3121 ctgctggtat agttatttta aaatcagaag gacagggaag ggagcagtgg ttcacgcctg
     3181 taatcccagc aatttgggag gccaaggtgg gtagatcacc tgagattagg agttggagac
     3241 cagcctggcc aatatggtga aaccccgtct ctaccaaaaa aacaaaaatt agctgagcct
     3301 ggtcatgcat gcctggaatc ccaacaactc gggaggctga ggcaggagaa tcgcttgaac
     3361 ccaggaggcg gagattgcag tgagccaaga ttgtgccact gcactccagc ttggttccca
     3421 atagaccccg caggccctac aggttgtctt cccaacttgc cccttgctcc ataccacccc
     3481 cctccacccc ataatattat agaaggacac ctagtcagac aaaatgatgc aacttaattt
     3541 tattaggaca aggctggtgg gcactggagt ggcaacttcc agggccagga gaggcactgg
     3601 ggaggggtca cagggatgcc acccgggatc ctctagatta ggaggccttg gtaagctgct
     3661 gaaggagaga gaggcctgca taaagagcac agaggcgagg gagggtgtct gggtcccaca
     3721 tatattgggg gcccgatctc tgcacttcca ggccactcag cccgactaga gcctccgtga
     3781 gggtcagctc ctcgtcccca agggaggaga gcagctcagg ttgcaggggg aaaacaccct
     3841 ctttatccag caggaagttc tgttccatcg tgctctccac cagctttagc tgcacgggta
     3901 ggatcttttt ctccatggat ttcaccagca gcttctgttg ggcttcactt agctctgtta
     3961 gggctccaac gaaatagagg atggcgccca cggcctcctt tgatagcagg acatcttttg
     4021 ggagtgcctc cagggtcact tcatgtccct tgtctagagc cccttcaagt gcgagctcaa
     4081 ggtcttgtag ctcctttttc ttccctagaa gtttgctgag ggagctgagc agggagcttt
     4141 gccctactcg gctcagcttc tccacttgtt gggtctctct ctgaacttct tcttttagtg
     4201 tcctgaagcc ttcgtgtacg tccccaacat cagatgcctg gataaggatg accttctcct
     4261 ctcctgactt ttctccagga gggaaggttt gcatgttatc attgcagata tgtggaatat
     4321 cccactcatc tttgcctttg accatcagct gtctcactcg aaaggccagg acgcagccct
     4381 tggggatggt tacagcctcc ttgtgattta tggatccctg tagccccaat ggggcgaaga
     4441 aggggaggga gaagcaggcc tctgccttgc cggctcgctc cagtgtgacc tcctgcaccg
     4501 tctccaccac ctccatcacc acatacaggt tctccccttg atcttgcatc tccttcagga
     4561 atgggtggtc tgctgccagc ttcctctcct gcacggtctc cagggccttg ggagccacac
     4621 tgagtgtctg gacctccaga gtgctgttct gcgagagccc tgccgttccc ttcaccttca
     4681 ccgtctttgg tacatccaca tctccctcca ctcgggtgtc cagcatattc ttaaagccaa
     4741 aattcccagt gtctgttggg tctgaaggtg agctgccggg ctcaagcaca tccagcagcg
     4801 tgtagtcggt gcggacgtac cgggcccccc agaagagcgt gctcttcctc ttcctcagca
     4861 ccaggcagaa gggatggaag cgcttgaagt cgatgaggct gtcaagtggt gtcaggtccc
     4921 ctcgagggtt tagctgtctg gccagggccc gggtgacatt ttcaaacatg gtcattgcgg
     4981 ccgcaagctt gtcgtcatcg tctttgtagt ccatggtaga tcgatctgaa ttaattctga
     5041 cggttcacta aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     5101 tgcgtcaacg gggcggggtt attacgacat tttggaaagt cccgttgatt ttggtgccaa
     5161 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     5221 tatccacgcc cattggtgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     5281 acgtagatgt actgccaagt aggaaagtcc cgtaaggtca tgtactgggc ataatgccag
     5341 gcgggccatt taccgtcatt gacgtcaata gggggcggac ttggcatatg atacacttga
     5401 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     5461 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     5521 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     5581 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa
     5641 catggcggtc atattggaca tgagccaata taaatgtaca tattatgata tagatacaac
     5701 gtatgcaatg gccaatagcc aatattgatt tatgctatat aaccaatgac taatatggct
     5761 aattgccaat attgattcaa tgtatagatc agcttggcac tggccgtcgt tttacaacgt
     5821 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5881 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5941 ctgaatggcg aatggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     6001 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     6061 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     6121 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     6181 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     6241 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     6301 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     6361 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     6421 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6481 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6541 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6601 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6661 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6721 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6781 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6841 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6901 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6961 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7021 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7081 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7141 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7201 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7261 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7321 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7381 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7441 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7501 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7561 tcaaaggatc ttc
//
