LOCUS       rerun_PCL380_GSDME_5    7726 bp    DNA     circular SYN 01-JUN-2025
DEFINITION  rerun_PCL380_GSDME_5.
ACCESSION   rerun_PCL380_GSDME_5
VERSION     rerun_PCL380_GSDME_5
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     polyA_signal    complement(3002..3624)
                     /note="pLannotate"
                     /label="hGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     rep_origin      complement(6136..6564)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(3641..5128)
                     /note="pLannotate"
                     /label="GSDME"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(2663..2960)
                     /note="pLannotate"
                     /label="SV40 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(5211..5410)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1818..2609)
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    complement(1029..1163)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        6591..6695
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(1417..1794)
                     /note="pLannotate"
                     /label="ble"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             6696..7556
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(5412..5791)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        1005..1023
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             5951..6105
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.1"
                     /fragment="True"
                     /other="CDS"
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(5145..5165)
                     /note="pLannotate"
                     /label="3xFLAG (fragment)"
                     /database="snapgene"
                     /identity="95.2"
                     /match_length="29.2"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1186..1269)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.9"
                     /fragment="True"
                     /other="CDS"
     rep_origin      1270..1293
                     /note="pLannotate"
                     /label="CloDF13 ori (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.2"
                     /fragment="True"
                     /other="rep_origin"
     CDS             complement(1316..1375)
                     /note="pLannotate"
                     /label="str (fragment)"
                     /database="swissprot"
                     /identity="95.0"
                     /match_length="7.5"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga catgattacg aatttaatac gactcactat
     1021 agggaattga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1081 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1141 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1201 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     1261 attatgatcg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttccagc
     1321 ttggcgagtg ggtggtgagc agctcgccgt cgcgcagcag gcgccagcgc tccatccacg
     1381 cagtgacctc aggcggaaaa cgggaagaca cactcatgag atgcctgcaa gcaattcgtt
     1441 ctgtatcagg cgcaggagcg tcccgtccgg gtcgaccaaa gcggccatcg tgcctcccca
     1501 ctcctgcagt tcgggggcat ggatgcgcgg atagccgctg ctggtttcct ggatgccgac
     1561 ggatttgcac tgccggtaga actccgcgag gtcgtccagc ctcaggcagc agctgaacca
     1621 actcgcgagg ggatcgagcc cggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1681 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1741 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1801 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1861 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1921 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1981 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     2041 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     2101 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     2161 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     2221 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     2281 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     2341 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     2401 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     2461 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2521 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2581 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2641 gatcccaagc tgatcaattc ctccaaaaaa gcctcctcac tacttctgga atagctcaga
     2701 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     2761 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     2821 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     2881 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     2941 gcctggggac tttccacacc ctaactgaca cacattccac agccaagctg gccgcgtacc
     3001 caattcaaca ggcatctact gagtggaccc aacgcatgag aggacagtgc caagcaagca
     3061 actcaaatgt cccaccggtt gggcatggcc aggtagccta tgctgtgtct ggacgtcctc
     3121 ctgctggtat agttatttta aaatcagaag gacagggaag ggagcagtgg ttcacgcctg
     3181 taatcccagc aatttgggag gccaaggtgg gtagatcacc tgagattagg agttggagac
     3241 cagcctggcc aatatggtga aaccccgtct ctaccaaaaa aacaaaaatt agctgagcct
     3301 ggtcatgcat gcctggaatc ccaacaactc gggaggctga ggcaggagaa tcgcttgaac
     3361 ccaggaggcg gagattgcag tgagccaaga ttgtgccact gcactccagc ttggttccca
     3421 atagaccccg caggccctac aggttgtctt cccaacttgc cccttgctcc ataccacccc
     3481 cctccacccc ataatattat agaaggacac ctagtcagac aaaatgatgc aacttaattt
     3541 tattaggaca aggctggtgg gcactggagt ggcaacttcc agggccagga gaggcactgg
     3601 ggaggggtca cagggatgcc acccgggatc ctctagatca tgaatgttct ctgcctaaag
     3661 cacagagtcc attcagggtt atacaaagaa gcagtgggaa gactttagag tccttcagaa
     3721 tgacagcttt cacagatgac ttcagtctct ccagactaat gtcagctgag gcaaacaagc
     3781 gctgcacaat cccaaacctt tctgtatctt tcaggggagt caaggttggg tcttcaagat
     3841 cagatactcc atcatcagac agagcacgaa gcaagtggca cagtgtggga atgatctgga
     3901 gtttgcagca agtgcccagc agagctgctg cgctatctgg catttctgcg agggcactga
     3961 ccaagaagta ggctgtcata aacagctgct tgctgcctgc atcctcgggg cccggacacc
     4021 caccctgtaa gctgcacccc accagctgca ggaaggccac aaggtcctgc tgctgccggg
     4081 gcttcagctc ccccagcacc gccactgtgg gcgagaggcc gctgaccagg tcatcgcaca
     4141 ctggttccag gaccatgagt agttcatcat caaataggac cgcctggaag atgtcactca
     4201 aagctgtctg ttgtggctca ggcagctccg caaatggatg gaaattcctc tccaggagca
     4261 gggtcgcttg ctttaaaaca cttaatggtc catcctggga agatatccca tgcgcagcat
     4321 ctggcatgtc tatgaatgca aactctcgaa agaccagggg gtccaggtag acagagtcaa
     4381 ttctcttctt gttctcgaag ccaccttgct tccctcggag aaggcagaac tcgaactggc
     4441 cgtccagttt cacgtataac tcaatgacac cgtaggcaat ggtggtggca gctgggatct
     4501 ccagcaccac gttggagtcc ttggtgacat tcccatcctc cgtcgctgac acctgcaccg
     4561 tcttggtctg gatgcccacg atgccaccac acttctcctc gacctgcatg tgctcagaga
     4621 tcacacactt ctgcatcgtc gtgatcttct gtgtcaaaac gcacaggacc tcattccttc
     4681 cttccagcac ctgctggagc acagggtttc tcagatttat tgttctctcg gcagagtctc
     4741 tgatgagctg ctgcaaatcc acctcctgct tcctcagggt tccaaatgaa gactggctct
     4801 ctacgcggct gctgcccccc aggttcagct tgaccttccc cagtgcagtc tccagggttc
     4861 cactcacgtg gtttgcaaac ttgccctcgt atttcacaaa gtccgactcc acgaccactg
     4921 gactcggaaa ttggtcttct atgagtacat cgccaagggt gagggataaa aactggtact
     4981 tgggtctctg ccagcaccag aatctcttct tttttgtcac cagacttaga agctgtaact
     5041 tatcagagtc attcagattt gatactgcaa tcaggtcacc atcagcatca acttctctaa
     5101 gaaaattcct ggttgctttg gcaaacattg cggccgcaag cttgtcgtca tcgtctttgt
     5161 agtccatggt agatcgatct gaattaattc tgacggttca ctaaacgagc tctgcttata
     5221 tagacctccc accgtacacg cctaccgccc atttgcgtca acggggcggg gttattacga
     5281 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
     5341 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattggt gtactgccaa
     5401 aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
     5461 tcccgtaagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
     5521 atagggggcg gacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
     5581 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
     5641 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
     5701 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
     5761 tactattaat aactagtcaa taatcaatgt caacatggcg gtcatattgg acatgagcca
     5821 atataaatgt acatattatg atatagatac aacgtatgca atggccaata gccaatattg
     5881 atttatgcta tataaccaat gactaatatg gctaattgcc aatattgatt caatgtatag
     5941 atcagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     6001 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     6061 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggaa attgtaagcg
     6121 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     6181 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     6241 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     6301 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     6361 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     6421 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     6481 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     6541 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6661 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6721 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6781 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6841 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6901 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6961 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     7021 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7081 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7141 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7201 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7261 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7321 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7381 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7441 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7501 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7561 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7621 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7681 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
