LOCUS       L.variegatus_polyubiquitinC_promoter        5672 bp    DNA     circular     29-MAR-2024
DEFINITION  .
ACCESSION   urn.local...1c-fe259qh
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Resistance markers: Ampicillin                                     
                                                                               
                                         This file was created using tools
            provided by Twist Bioscience.
FEATURES             Location/Qualifiers
     promoter        7..3445
                     /created_by="elliotjackson"
                     /X-Transferred_From="L.variegatus_polyubiquitinC in pTwist
                     Amp High Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:9d-etrct8q"">L.variegatus_polyubiquitin
                     C in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:br-etrdi3a"">polyubi C promoter +
                     mRFP</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:5w-eylipru"">H2B Cerulean plasmid
                     (backbone unknown) - L.variegatus_polyubiquitinC in pTwist
                     Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:84-ervi19a"">Genomic segment with
                     L.variegatus polyubiquitinC extraction. SEQUENCE TO
                     SYNTHESIZE</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RFP_mChilada_LCK in pCS2_8 -
                     L.variegatus_polyubiquitinC in pTwist Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     L.variegatus polyubiquitin C promoter (<a
                     href=""urn:local:.:3n-ev0i2rr"">RFP_mChilada_LCK in pCS2_8
                     - L.variegatus_polyubiquitinC in pTwist Amp High
                     Copy</a>)"
                     /label="L.variegatus polyubiquitin C promoter"
     misc_feature_   3508..3524
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RFP_mChilada_LCK in pCS2_8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     m13_reverse (<a
                     href=""urn:local:.:36-ezb4qur"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /label="m13_reverse"
     primer_R        complement(3508..3524)
                     /created_by="elliotjackson"
                     /X-Transferred_From="UTR_NPRE_mChilada"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13R
                     (<a href=""urn:local:.:n-f70e0xc"">UTR_NPRE_mChilada</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R (<a
                     href=""urn:local:.:2a-f70fv8q"">UTR_Nanos2_mChilada</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Lp-pabp-exon1-3000 in pUC-GW-Amp"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13R (<a
                     href=""urn:local:.:3f-f713nqy"">Lp-pabp-exon1-3000 in
                     pUC-GW-Amp</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13R
                     (<a
                     href=""urn:local:.:5b-f7148te"">Lp-EGF1alpha-exon2-3000 in
                     pUC-GW-Amp</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13R
                     (<a
                     href=""urn:local:.:4y-f7145hb"">Lp-tubulin-beta-exon1-2000
                     in pUC-GW-Amp</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13R
                     (<a
                     href=""urn:local:.:5q-f714cm3"">Lp-EGF1alpha_exon1-3000 in
                     pUC-GW-Amp</a>)"
                     /label="M13R"
     primer_bind     complement(3508..3524)
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /X-Transferred_From="pabp in pUC-GW-Amp"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13R
                     (<a href=""urn:local:.:41-ev0j5p0"">pabp in
                     pUC-GW-Amp</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R (<a href=""urn:local:.:ca-euqjr84"">pUC-GW-Amp.dna -
                     L.pictus Nanos 3' and 5'  w/o ORF -
                     RFP_mChilada_Nanos_NPRE in pCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R (<a href=""urn:local:.:5w-eylipru"">H2B Cerulean
                     plasmid (backbone unknown) - L.variegatus_polyubiquitinC
                     in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R (<a href=""urn:local:.:52-ev0judx"">RFP_mChilada_LCK
                     in pCS2_8 - pabp in pUC-GW-Amp</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R (<a href=""urn:local:.:d-ey08kw3"">Pictus Nanos  UTR
                     MCS in pUC-GW-Amp</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R (<a
                     href=""urn:local:.:1r-euq9nvm"">pUC-GW-Amp.dna</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R (<a
                     href=""urn:local:.:dj-euqq8ab"">PhiC_31_Minos_ITR_MCS in
                     pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R (<a href=""urn:local:.:n-eurzkb4"">Nanos 5' and 3'
                     UTR </a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R (<a href=""urn:local:.:5k-epr10c4"">RFP_mChilada_LCK
                     in pCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13R (<a href=""urn:local:.:5c-epr10bm"">H2B_RFP_mChilada
                     in pCS2_8</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="YFP_mAbbsynth_LCK in pCS2_8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13R (<a
                     href=""urn:local:.:3s-ezb51fb"">YFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /label="M13R"
     primer_R        complement(3521..3543)
                     /created_by="elliotjackson"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RFP_mChilada_LCK in pCS2_8 -
                     L.variegatus_polyubiquitinC in pTwist Amp High Copy"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13/pUC
                     R (<a href=""urn:local:.:3n-ev0i2rr"">RFP_mChilada_LCK in
                     pCS2_8 - L.variegatus_polyubiquitinC in pTwist Amp High
                     Copy</a>)"
                     /label="M13/pUC R"
     primer_bind     complement(3521..3543)
                     /Tm=58
                     /Sequence="AGCGGATAACAATTTCACACAGG"
                     /Description="In lacZ gene"
                     /X-Transferred_From="PhiC 31 Minos ITR MCS in pTwist Amp
                     High Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a href=""urn:local:.:8o-etrc9l3"">PhiC
                     31 Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a
                     href=""urn:local:.:6l-etqsdfk"">RFP_mChilada_Nanos_NPRE in
                     pCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a href=""urn:local:.:5j-etqqqce"">PhiC
                     31 Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RFP_mChilada_LCK in pCS2_8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13/pUC
                     Reverse (<a
                     href=""urn:local:.:36-ezb4qur"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a
                     href=""urn:local:.:3s-ezb51fb"">YFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     M13/pUC Reverse (<a
                     href=""urn:local:.:29-ezb4d4i"">GFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /label="M13/pUC Reverse"
     rep_origin      complement(3696..4284)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; ori (<a
                     href=""urn:local:.:4p-ezuyafv"">pCS2+8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ori
                     (<a href=""urn:local:.:36-ezb4qur"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ori
                     (<a href=""urn:local:.:3s-ezb51fb"">YFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ori
                     (<a href=""urn:local:.:4c-ezuy8fo"">mEGFP_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ori
                     (<a href=""urn:local:.:29-ezb4d4i"">GFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; ori
                     (<a href=""urn:local:.:4g-ezuy8g3"">mNeonGreen_LCK in
                     pCS2_8</a>)"
                     /label="ori"
     primer_bind     complement(3776..3795)
                     /Tm=56
                     /Sequence="GGGAAACGCCTGGTATCTTT"
                     /Description="pBR322 origin, forward primer"
                     /X-Transferred_From="PhiC 31 Minos ITR MCS in pTwist Amp
                     High Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:6l-etqsdfk"">RFP_mChilada_Nanos_NPRE in
                     pCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a href=""urn:local:.:5j-etqqqce"">PhiC 31
                     Minos ITR MCS in pTwist Amp High Copy</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RFP_mChilada_LCK in pCS2_8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:36-ezb4qur"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:3s-ezb51fb"">YFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     pBR322ori-F (<a
                     href=""urn:local:.:29-ezb4d4i"">GFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /label="pBR322ori-F"
     attB1           complement(4404..4424)
     CDS             complement(4525..5385)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /Transferred_Translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVK
                     DAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLG
                     RRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTA
                     FLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLI
                     DWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGS
                     QATMDERNRQIAEIGASLIKHW"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCS2+8"
                     /Transferred_Similarity="99.88%"
                     /Primary_Match="99.88%; Adjusted-Interval-0:0/0; AmpR (<a
                     href=""urn:local:.:4p-ezuyafv"">pCS2+8</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; AmpR
                     (<a href=""urn:local:.:36-ezb4qur"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; AmpR
                     (<a href=""urn:local:.:3s-ezb51fb"">YFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; AmpR
                     (<a href=""urn:local:.:4c-ezuy8fo"">mEGFP_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; AmpR
                     (<a href=""urn:local:.:29-ezb4d4i"">GFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="99.88%; Adjusted-Interval-0:0/0; AmpR
                     (<a href=""urn:local:.:4g-ezuy8g3"">mNeonGreen_LCK in
                     pCS2_8</a>)"
                     /label="AmpR"
     primer_bind     5148..5167
                     /Tm=58
                     /Sequence="ATAATACCGCGCCACATAGC"
                     /Description="Ampicillin resistance gene, reverse primer"
                     /X-Transferred_From="PhiC 31 Minos ITR MCS in pTwist Amp
                     High Copy"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; Amp-R
                     (<a href=""urn:local:.:8o-etrc9l3"">PhiC 31 Minos ITR MCS
                     in pTwist Amp High Copy</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a
                     href=""urn:local:.:hv-elvr0dd"">pBlueSKMimRNA</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a
                     href=""urn:local:.:6l-etqsdfk"">RFP_mChilada_Nanos_NPRE in
                     pCS2_8</a>)"
                     /X-Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a href=""urn:local:.:5j-etqqqce"">PhiC 31 Minos
                     ITR MCS in pTwist Amp High Copy</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="RFP_mChilada_LCK in pCS2_8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; Amp-R
                     (<a href=""urn:local:.:36-ezb4qur"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a
                     href=""urn:local:.:3s-ezb51fb"">YFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0;
                     Amp-R (<a
                     href=""urn:local:.:29-ezb4d4i"">GFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /label="Amp-R"
     promoter        complement(5386..5490)
                     /gene="bla"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pCS2+8"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:4p-ezuyafv"">pCS2+8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:36-ezb4qur"">RFP_mChilada_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:3s-ezb51fb"">YFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a href=""urn:local:.:4c-ezuy8fo"">mEGFP_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:29-ezb4d4i"">GFP_mAbbsynth_LCK in
                     pCS2_8</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; AmpR
                     promoter (<a
                     href=""urn:local:.:4g-ezuy8g3"">mNeonGreen_LCK in
                     pCS2_8</a>)"
                     /label="AmpR promoter"
     primer_bind     5603..5620
                     /Tm=56
                     /Sequence="TGTAAAACGACGGCCAGT"
                     /Description="In lacZ gene. Also called M13-F20 or M13
                     (-21) Forward"
                     /X-Transferred_From="Lv-pabp in pUC-GW-Amp"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a href=""urn:local:.:41-ev0j5p0"">Lv-pabp in
                     pUC-GW-Amp</a>)"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Lp-pabp-exon1-3000 in pUC-GW-Amp"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a
                     href=""urn:local:.:3f-f713nqy"">Lp-pabp-exon1-3000 in
                     pUC-GW-Amp</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a
                     href=""urn:local:.:5b-f7148te"">Lp-EGF1alpha-exon2-3000 in
                     pUC-GW-Amp</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a
                     href=""urn:local:.:4y-f7145hb"">Lp-tubulin-beta-exon1-2000
                     in pUC-GW-Amp</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a
                     href=""urn:local:.:5q-f714cm3"">Lp-EGF1alpha_exon1-3000 in
                     pUC-GW-Amp</a>)"
                     /Alternative_Match="100.00%; Adjusted-Interval-0:0/0; M13
                     Forward (<a href=""urn:local:.:41-ev0j5p0"">Lv-pabp in
                     pUC-GW-Amp</a>)"
                     /label="M13 Forward"
ORIGIN      
        1 aagcttacta gtcctcacaa catttcttcc cttttttgcg ggagttgggg caaataatcc
       61 tgacaatgtc caatgattat tttaaaaagt gctatgtaca atcgctttgt atttgtataa
      121 ttgttataaa aaatgcattg tatgtattta tatttacata cataattatt ctacaaagtg
      181 tattgtacaa tcactttata tcaactttag atgtatcgtg ttattagtgt caaatctgac
      241 tgcgtgcata ctgattcgat gccaaaagtg gattaatgcc tccttgtgcc ccgcgtgtct
      301 cttggcaact agagcattat tgtagaatat tggggcacct aaaatacact gacaagcaat
      361 catccaaaaa tatctaacaa aatactaaac aataacagga cggtcatttt tctgatatct
      421 aaaacaagtt tttgttgaag ggacattgtg caagactttc aaaaacttat taagaatgtt
      481 gaaccgttta ctttttttat tttaacacgt gatactatag tatacataac gattgggcga
      541 aaaaataccg tgacagtcaa tcaaacaatc taaattccca ctttgcagaa tcacttttgt
      601 gattcccttg tatcttcaac aagatataga tagatggata gcatggaagt ttttgttttt
      661 tacttagaaa tggtaaaacg atgttttttc cccagggtct tttcccccta tgtaaattat
      721 gtttcattct ttcaataaaa aaaaacacaa actgaaaaaa aacttataag caaaaaaaaa
      781 ggcgtaaata tttttttatg aactctgtta aacggaaaca acaacaacac agtcgccaat
      841 atatacctcc cgcgaccagt gatcattcca gaaaatgttt tgggccgaaa ttatattgca
      901 taattccgaa attgcgagtc atcatcgtct gcaatatctg gcgcgggtta atatgacgtc
      961 atgtgccata gatacacaat ctatctttat tacccaatag aaaaggatga aatagtccat
     1021 gtgacacaga ctgaccaatc gcaagtcaac acaaagcacc atataaaaca cacgcaagcg
     1081 tcgcagtgtc aataaattgc atgagacttc cgcagaggtt gcactcgaga agaactcgtc
     1141 catttttttc cagaaaaact ctaatttcaa agtaagtaga cgaggttttc acattatttt
     1201 aaggcagtta tttcaatgaa gatgcagttt gtaaatgttt gatttaaaaa aagcactttt
     1261 gaagaagaat gatgatgaag aacataaatt gtcaacgacc acatcgtcgt cattattcgt
     1321 gtgctttttt tttgttgtaa tttatgtttg gctttccaaa tgaaggcaat ataggaacat
     1381 taacagcatg aacaaagtct catatttagt ttgattgaaa gttcaaatta tagggcgtag
     1441 aaatcattga aattgtaggg tggataccgt ggattattgc gtcgtgtgtg tgcatgataa
     1501 gatcgtctga ctcagacgtc gcgtcttttg ttgtgtggtg tgtgtggtat ggatgatgat
     1561 caattgtgat gtttggtggg gattttcatg aacgctgaat taaaagacaa tgagtggaaa
     1621 tggccatgcc aatttctggt ccatggattg tgtgaatgca atgacaattt cattcaattt
     1681 ttgtctgggc tgccatgatg aacatgatga agtctgaact gaagtgttgt gattgaaatg
     1741 acaaagaatt tttgtcatga gaaagaatga ggcatgtacg gtacggtaac ttaaaacgtt
     1801 gatagctagg tgtagatttt ctagatacgt ttggcagtag gcctagatct agaggcactt
     1861 ggccaatcat gaatagtcta tgggagacaa tttcccatat tgaagacttg ctttgcaaaa
     1921 gaaacgacac caacaaagcg taattttttc caccccaaat tttttcgcga aggtcagaac
     1981 agaagcccat ggcaaaggcc tgttttgtat gtgcatggcc atggatgaaa aaaatataaa
     2041 tacaaaagtt ggtgaatttt taaagatgaa aaggggtaat ttttcatgag gtatctgctt
     2101 aggccctatc acatttttat ttgctgccaa ggtaattctt ttgctgcatg tgtaaaaaaa
     2161 ggaaattggt ctcccaaact gggagtggag actgtctctg aaattggata cccaagttct
     2221 ttaatcttta caaaattctg atattgaaaa taatctctct catgggagat gttctcccat
     2281 attggctgtg caccagttgg tcggtgtgca aagtctagac aatacgtatc caggcactat
     2341 cgcctatgaa taatgcaata gcagtgcagt tcacaacggc ctctctcccg atcagcgcag
     2401 atgttgggga actaatttca acttttgtcg taatttatgt caaataactg acagtcgtaa
     2461 cttaagaccc taatcagcta atgttaatcc ttgacctgaa ttgcaggcat ctgaaacggc
     2521 tgtaatccag cttcgatctt aatgtcatag ttcaaattct cagtagattt aactgagcgt
     2581 ggtgcgtgtt tggccattgc catagttttt atatggactg aagtcaacga ccaaaagatc
     2641 tgctcaaggc agcccaactg cattagcaag attttggttg ctattcagtc aatatcagaa
     2701 ctacggcaat ggacgagtgc ggcgatgtca acctacatgt aactgagaat tcaaactatg
     2761 acattgagaa cgaagccaaa atacagcaaa tattgatgtg gttgaaactt gggtcaagga
     2821 tcaaatggac aatatttggc aaaaaagata aattttgagg tgaaattgtt tcctgacatt
     2881 gccatgttgg ttggtgtgaa actgcagcgc aagacaatga aggaagagac tttatagagt
     2941 aagctgcccc gaatttgaca tgttttgaac cattttttgt tcttaagtct tttcacataa
     3001 tgaaaattgc attaaaatga tcaatgttgg ttgaatatat ttagaattgc aatgttgaat
     3061 ttctccattt tgaatatttc ataaaaaatc tgcactgtac caccatgttt tgctcccgcc
     3121 acagatttct taattttaga gcattttatg gctacctcat ttcttaaatc tgagcaaagc
     3181 taccgcatgt tattgaaata tggcacagat tcgcattttt aaaggggatt ttggtgttca
     3241 agtcgtgact tgcatgtggt gggtgataat gcctgtgtat gtcaatgcat gtggttcgtg
     3301 ttgtgtggat tgactgagct gacttgtgga ataagtttgc catgttgaat tccaggcatg
     3361 ctactctgaa taaaggtctt tagaaaaaca gattccaata atgttttcat ttttttgtca
     3421 attttctaca ggtcagtgcg atcgcatcga taggctaggt ggaggctcag tgatgataag
     3481 tctgcgatgg tggatgcatg tgtcatggtc atagctgttt cctgtgtgaa attgttatcc
     3541 gctcagaggg cacaatccta ttccgcgcta tccgacaatc tccaagacat taggtggagt
     3601 tcagttcggc gtatggcata tgtcgctgga aagaacatgt gagcaaaagg ccagcaaaag
     3661 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3721 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3781 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3841 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3901 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3961 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     4021 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4081 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     4141 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4201 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4261 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4321 ctattcaaca aagccgccgt cccgtcaagt cagcgtaaat gggtaggggg cttcaaatcg
     4381 tcctcgtgat accaattcgg agcctgcttt tttgtacaaa cttgttgata atggcaattc
     4441 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     4501 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     4561 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4621 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagagc cacgctcacc
     4681 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4741 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4801 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4861 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4921 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4981 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     5041 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     5101 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     5161 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     5221 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     5281 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     5341 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     5401 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     5461 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cagatacctg
     5521 aaacaaaacc catcgtacgg ccaaggaagt ctccaataac tgtgatccac cacaagcgcc
     5581 agggttttcc cagtcacgac gttgtaaaac gacggccagt catgcataat ccgcacgcat
     5641 ctggaataag gaagtgccat tccgcctgac ct
//
