LOCUS       Exported                7839 bp ds-DNA     circular SYN 02-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Stanford University
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Apr 1, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        494..598
                     /gene="bla"
                     /label=AmpR promoter
     CDS             599..1459
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1275..1302)
                     /label=Primer 2
     primer_bind     1303..1325
                     /label=Primer 1
                     /note="Mutagenesis Primer"
     primer_bind     1303..1325
                     /label=Mutagenic Primer
     rep_origin      1630..2218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2648..2839)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3648..4730)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(4731..4808)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        5117..5135
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5136..5160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5191..5196
                     /note="ribosome binding site"
     CDS             5205
                     /codon_start=1
                     /label=ATG
                     /translation=""
     CDS             5205
                     /codon_start=1
                     /label=Expressed Protein
                     /translation=""
     misc_feature    5206..5465
                     /label=GA Insert 1
     primer_bind     5226..5251
                     /label=Frag3Forward
     primer_bind     5226..5251
                     /label=Frag3Forwarda
     primer_bind     5226..5251
                     /label=Frag3Forward`b
     primer_bind     5229..5248
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     5280..5293
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     complement(5442..5469)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(5442..5464)
                     /label=Frag2Rev
     primer_bind     complement(5442..5464)
                     /label=Frag2Reva
     primer_bind     complement(5442..5464)
                     /label=Frag2Rev`b
     primer_bind     complement(5457..5470)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(5457..5470)
                     /label=Frag2Rev
     primer_bind     complement(5457..5470)
                     /label=Frag2Reva
     primer_bind     complement(5457..5470)
                     /label=Frag2Rev`b
     primer_bind     5466..5491
                     /label=Frag3Forward
     primer_bind     5466..5491
                     /label=Frag3Forwarda
     primer_bind     5466..5491
                     /label=Frag3Forward`b
     primer_bind     5466..5488
                     /label=Frag2For
                     /note="ForwardAnnealing"
     misc_feature    5469..5702
                     /label=GA Insert 1
     primer_bind     5520..5533
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     complement(5682..5706)
                     /label=Frag2Rev
     primer_bind     complement(5682..5706)
                     /label=Frag2Reva
     primer_bind     complement(5682..5706)
                     /label=Frag2Rev`b
     primer_bind     complement(5682..5704)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     5703..5731
                     /label=Frag3Forwarda
     primer_bind     5703..5731
                     /label=Frag3Forward`b
     misc_feature    5706..6028
                     /label=GA Insert 1
     primer_bind     5706..5731
                     /label=Frag3Forward
     primer_bind     5709..5728
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     5760..5773
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     complement(5922..5947)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(5922..5944)
                     /label=Frag2Rev
     primer_bind     complement(5922..5944)
                     /label=Frag2Reva
     primer_bind     complement(5922..5944)
                     /label=Frag2Rev`b
     primer_bind     5979..5996
                     /label=V_InsertFor
     primer_bind     complement(6019..6032)
                     /label=Frag3Reverse
     misc_feature    6029..6373
                     /label=GA Insert 2
     primer_bind     complement(6087..6106)
                     /label=Frag2Rev
     primer_bind     complement(6087..6106)
                     /label=Frag2Reva
     primer_bind     complement(6087..6106)
                     /label=Frag2Rev`b
     primer_bind     complement(6102..6129)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(6102..6124)
                     /label=Frag2Rev
     primer_bind     complement(6102..6124)
                     /label=Frag2Reva
     primer_bind     complement(6102..6124)
                     /label=Frag2Rev`b
     primer_bind     complement(6117..6144)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(6117..6130)
                     /label=Frag2Rev
     primer_bind     complement(6117..6130)
                     /label=Frag2Reva
     primer_bind     complement(6117..6130)
                     /label=Frag2Rev`b
     primer_bind     6189..6208
                     /label=Frag2For
                     /note="ForwardAnnealing"
     misc_feature    6374..6768
                     /label=GA_Insert3
     primer_bind     6525..6538
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     6527..6556
                     /label=Frag3Forwarda
     primer_bind     6527..6556
                     /label=Frag3Forward`b
     primer_bind     6531..6556
                     /label=Frag3Forward
     primer_bind     6584..6601
                     /label=Frag3Forward
     primer_bind     6584..6601
                     /label=Frag3Forwarda
     primer_bind     6584..6601
                     /label=Frag3Forward`b
     primer_bind     complement(6597..6619)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(6597..6619)
                     /label=Frag2Rev
     primer_bind     complement(6597..6619)
                     /label=Frag2Reva
     primer_bind     complement(6597..6619)
                     /label=Frag2Rev`b
     primer_bind     complement(6627..6645)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(6627..6645)
                     /label=Frag2Rev
     primer_bind     complement(6627..6645)
                     /label=Frag2Reva
     primer_bind     complement(6627..6645)
                     /label=Frag2Rev`b
     CDS             6679..6756
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="YFLPVPRWCSPRYASVRYFTPHLVE"
     CDS             complement(6690..6714)
                     /codon_start=1
                     /label=Expressed Protein
                     /translation="EDYTIVEQ"
     CDS             complement(6690..6714)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="EDYTIVEQ"
     CDS             6752..6757
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="EL"
     misc_feature    6773..7126
                     /label=GA Insert 4
     primer_bind     6777..6797
                     /label=V_InsertFor
     primer_bind     complement(6874..6890)
                     /label=V_insertREV
     primer_bind     complement(7114..7130)
                     /label=V_insertREV
     misc_feature    7127..7462
                     /label=GA Insert 5
     misc_feature    7463..7681
                     /label=Last Insert
     primer_bind     7467..7492
                     /label=Frag3Forward
     primer_bind     7467..7492
                     /label=Frag3Forwarda
     primer_bind     7467..7492
                     /label=Frag3Forward`b
     primer_bind     7478..7505
                     /label=Primer 1a
                     /note="FWD"
     primer_bind     7542..7565
                     /label=Primer 1a
                     /note="FWD"
     primer_bind     7628..7655
                     /label=Primer 1a
                     /note="FWD"
     CDS             7682..7703
                     /codon_start=1
                     /label=Expressed Protein
                     /translation="HHHHHH"
     misc_feature    7682
                     /label=MCS
                     /note="multiple cloning site"
     CDS             7683..7700
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      7767..7814
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      601 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      661 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      721 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      781 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
      841 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
      901 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
      961 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1021 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1081 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1141 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1201 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1261 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg
     1321 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1381 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1441 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     1501 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     1561 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     1621 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     1681 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     1741 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     1801 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     1861 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     1921 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     1981 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2041 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2101 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2161 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2221 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     2281 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     2341 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     2401 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg
     2461 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     2521 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     2581 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     2641 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct
     2701 catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt
     2761 tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg
     2821 ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa
     2881 tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc
     2941 ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa
     3001 aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta
     3061 gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg
     3121 tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag
     3181 acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac
     3241 cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca
     3301 cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
     3361 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
     3421 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
     3481 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
     3541 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag
     3601 atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt
     3661 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3721 gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc
     3781 tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc
     3841 cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct
     3901 tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta
     3961 atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg
     4021 atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct
     4081 tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga
     4141 cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc
     4201 aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg
     4261 ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct
     4321 tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt
     4381 tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc
     4441 gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc
     4501 gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc
     4561 gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact
     4621 ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga
     4681 taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc
     4741 ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg
     4801 atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag
     4861 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
     4921 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
     4981 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
     5041 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
     5101 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
     5161 cctctagaaa taattttgtt taactttaag aaggagatat acatatgggt ggttggggac
     5221 cagtgcctgg cgtcggtgta cctggtgctg gtgtacccgg tgctggagta ccgggggcag
     5281 gtgtaccagg tgcaggggtc ccaggagctg gagttccagg tgctggggtt cctggtgcag
     5341 gtgttcctgg agcaggtgtt cctggtggcg gagttcctgg cggtggggtg cctggtgggg
     5401 gcgtgccagg cgggggcgtt cccggtggcg gtgtccccgg tggtggggtt ccagggggtg
     5461 gggtaccagg cgtcggtgta cctggtgctg gtgtacccgg tgctggagta ccgggggcag
     5521 gtgtaccagg tgcaggggtc ccaggagctg gagttccagg tgctggggtt cctggtgcag
     5581 gtgttcctgg agcaggtgtt cctggtggcg gagttcctgg cggtggggtg cctggtgggg
     5641 gcgtgccagg cgggggcgtt cccggtggcg gtgtccccgg tggtggggtt ccagggggtg
     5701 gggtccctgg cgtcggtgta cctggtgctg gtgtacccgg tgctggagta ccgggggcag
     5761 gtgtaccagg tgcaggggtc ccaggagctg gagttccagg tgctggggtt cctggtgcag
     5821 gtgttcctgg agcaggtgtt cctggtggcg gagttcctgg cggtggggtg cctggtgggg
     5881 gcgtgccagg cgggggcgtt cccggtggcg gtgtccccgg tggtggggtt ccagggggtg
     5941 gggtaccggg agtgggcgtc ccaggtgcgg gcgttcccgg cgcaggtgtg cctggagcgg
     6001 gagtaccagg agccggcgtg ccaggggcgg gtgtaccagg ggccggagtg ccaggtgctg
     6061 gagtaccggg cgctggcgtt cctggtggtg gcgttccggg tggtggagtt cccggtggtg
     6121 gggttccagg tgggggagta ccaggcggtg gagtccctgg cggcggtgta ccaggtggtg
     6181 gtgtgcctgg cgtcggtgta cccggtgcag gggttccagg agcgggagtt cctggtgcgg
     6241 gtgtgccggg ggctggtgta cctggagcag gtgttccggg agctggggtc cctggggcag
     6301 gagtcccagg cgcaggcgtc ccagggggtg gagtgccggg cgggggtgtg ccaggtggcg
     6361 gagtacctgg gggcggagta ccaggcggtg gtgtgccggg cggcggtgta ccgggtggcg
     6421 gggtacccgg tgtgggagtt ccgggagctg gcgtcccggg tgcaggagtc ccaggggctg
     6481 gagtgccagg agcaggcgta ccaggggcgg gagtccccgg agctggtgtc cctggtgccg
     6541 gggtaccggg tgctggcgta ccagggggcg gtgtaccagg tggcggcgta cctggtggtg
     6601 gagttccagg cggcggggtt cctgggggtg gtgttccagg tgggggtgtt cccggcggcg
     6661 gttctggtgg cggagaccgt attttctccc tgttccacga tggtgtagtc ctcgttacgc
     6721 atctgtgcgg tatttcacac cgcatttggt ggagctcggt ctctggcggc tccgtgccag
     6781 gcgtcggtgt gcctggagtc ggggtgccag gggtaggagt ccctggcgta ggagtgccag
     6841 gagtgggtgt tccgggtgta ggagttcctg gagtgggggt tcccggtgtt ggcgtaccgg
     6901 gcgttggagt acctggtgta ggtgtgcccg gtgtaggcgt gcctggcgtt ggtgttccag
     6961 gagttggggt ccccggagtt ggcgttccag gtgtcggggt tccgggggtt ggagtgcctg
     7021 gtgtgggcgt acctggagta ggagtacccg gtgtgggagt gccgggagtt ggagtcccag
     7081 gggtcggcgt tcctggggtg ggagtcccgg gggtgggcgt tcccggagtt ggggtgcccg
     7141 gagtaggcgt accaggcgtg ggagtaccgg gtgtgggggt cccaggtgta ggggtacccg
     7201 gcgtgggtgt gccgggtgtc ggagttcccg gagtcggagt ccccggggtg ggtgtaccgg
     7261 gggtcggggt accaggggtg ggggtgccgg gggtaggcgt tccgggcgta ggggttcctg
     7321 gtgtcggtgt tcccggcgta ggtgttcctg gcgtgggggt accgggagtg ggcgtcccgg
     7381 gtgttggagt tccgggagta ggtgtacccg gagtgggagt tccaggcgtt ggggttccag
     7441 gggttggcgt cccaggagta ggtgtacctg gggtaggtgt ccctggtgtt ggtgtacctg
     7501 gcgtcggagt gcccggggtt ggggtgcctg gggttggtgt gccaggtgtt ggggtacctg
     7561 gggtcggagt accaggagtc ggtgtaccag gtgtgggtgt cccaggcgta ggcgtccctg
     7621 gagttggtgt ccccggtgtc ggcgtacccg gggtcggtgt cccgggagtc ggctggctcg
     7681 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
     7741 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     7801 tgaggggttt tttgctgaaa ggaggaacta tatccggat
//
