LOCUS       Exported                7773 bp ds-DNA     circular SYN 11-NOV-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7773)
  AUTHORS   Stanford University
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Apr 1, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7773
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..31
                     /label=Insert 3
     CDS             4..24
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     misc_feature    36..389
                     /label=GA Insert 4
     primer_bind     40..60
                     /label=V_InsertFor
     primer_bind     complement(137..153)
                     /label=V_insertREV
     primer_bind     complement(377..393)
                     /label=V_insertREV
     misc_feature    390..725
                     /label=GA Insert 5
     misc_feature    726..944
                     /label=Last Insert
     primer_bind     730..755
                     /label=Frag3Forward
     primer_bind     730..755
                     /label=Frag3Forwarda
     primer_bind     730..755
                     /label=Frag3Forward`b
     primer_bind     741..768
                     /label=Primer 1a
                     /note="FWD"
     primer_bind     805..828
                     /label=Primer 1a
                     /note="FWD"
     primer_bind     891..918
                     /label=Primer 1a
                     /note="FWD"
     CDS             945..966
                     /codon_start=1
                     /label=Expressed Protein
                     /translation="HHHHHH"
     misc_feature    945
                     /label=MCS
                     /note="multiple cloning site"
     CDS             946..963
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1030..1077
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1114..1569
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        1596..1700
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1701..2561
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2377..2404)
                     /label=Primer 2
     primer_bind     2405..2427
                     /label=Primer 1
                     /note="Mutagenesis Primer"
     primer_bind     2405..2427
                     /label=Mutagenic Primer
     rep_origin      2732..3320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3750..3941)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(4750..5832)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(5833..5910)
                     /gene="lacI"
                     /label=lacI promoter
     promoter        6219..6237
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6238..6262
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6293..6298
                     /note="ribosome binding site"
     CDS             6307
                     /codon_start=1
                     /label=ATG
                     /translation=""
     CDS             6307
                     /codon_start=1
                     /label=Expressed Protein
                     /translation=""
     misc_feature    6308..6567
                     /label=GA Insert 1
     primer_bind     6328..6353
                     /label=Frag3Forward
     primer_bind     6328..6353
                     /label=Frag3Forwarda
     primer_bind     6328..6353
                     /label=Frag3Forward`b
     primer_bind     6331..6350
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     6382..6395
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     complement(6544..6571)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(6544..6566)
                     /label=Frag2Rev
     primer_bind     complement(6544..6566)
                     /label=Frag2Reva
     primer_bind     complement(6544..6566)
                     /label=Frag2Rev`b
     primer_bind     complement(6559..6572)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(6559..6572)
                     /label=Frag2Rev
     primer_bind     complement(6559..6572)
                     /label=Frag2Reva
     primer_bind     complement(6559..6572)
                     /label=Frag2Rev`b
     primer_bind     6568..6593
                     /label=Frag3Forward
     primer_bind     6568..6593
                     /label=Frag3Forwarda
     primer_bind     6568..6593
                     /label=Frag3Forward`b
     primer_bind     6568..6590
                     /label=Frag2For
                     /note="ForwardAnnealing"
     misc_feature    6571..6804
                     /label=GA Insert 1
     primer_bind     6622..6635
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     complement(6784..6808)
                     /label=Frag2Rev
     primer_bind     complement(6784..6808)
                     /label=Frag2Reva
     primer_bind     complement(6784..6808)
                     /label=Frag2Rev`b
     primer_bind     complement(6784..6806)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     6805..6833
                     /label=Frag3Forwarda
     primer_bind     6805..6833
                     /label=Frag3Forward`b
     misc_feature    6808..7130
                     /label=GA Insert 1
     primer_bind     6808..6833
                     /label=Frag3Forward
     primer_bind     6811..6830
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     6862..6875
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     complement(7024..7049)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(7024..7046)
                     /label=Frag2Rev
     primer_bind     complement(7024..7046)
                     /label=Frag2Reva
     primer_bind     complement(7024..7046)
                     /label=Frag2Rev`b
     primer_bind     7081..7098
                     /label=V_InsertFor
     primer_bind     complement(7121..7134)
                     /label=Frag3Reverse
     misc_feature    7131..7475
                     /label=GA Insert 2
     primer_bind     complement(7189..7208)
                     /label=Frag2Rev
     primer_bind     complement(7189..7208)
                     /label=Frag2Reva
     primer_bind     complement(7189..7208)
                     /label=Frag2Rev`b
     primer_bind     complement(7204..7231)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(7204..7226)
                     /label=Frag2Rev
     primer_bind     complement(7204..7226)
                     /label=Frag2Reva
     primer_bind     complement(7204..7226)
                     /label=Frag2Rev`b
     primer_bind     complement(7219..7246)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(7219..7232)
                     /label=Frag2Rev
     primer_bind     complement(7219..7232)
                     /label=Frag2Reva
     primer_bind     complement(7219..7232)
                     /label=Frag2Rev`b
     primer_bind     7291..7310
                     /label=Frag2For
                     /note="ForwardAnnealing"
     misc_feature    7476..7773
                     /label=GA_Insert3
     primer_bind     7627..7640
                     /label=Frag2For
                     /note="ForwardAnnealing"
     primer_bind     7629..7658
                     /label=Frag3Forwarda
     primer_bind     7629..7658
                     /label=Frag3Forward`b
     primer_bind     7633..7658
                     /label=Frag3Forward
     primer_bind     7686..7703
                     /label=Frag3Forward
     primer_bind     7686..7703
                     /label=Frag3Forwarda
     primer_bind     7686..7703
                     /label=Frag3Forward`b
     primer_bind     complement(7699..7721)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(7699..7721)
                     /label=Frag2Rev
     primer_bind     complement(7699..7721)
                     /label=Frag2Reva
     primer_bind     complement(7699..7721)
                     /label=Frag2Rev`b
     primer_bind     complement(7729..7747)
                     /label=Frag1Rev
                     /note="Reverse Annealing"
     primer_bind     complement(7729..7747)
                     /label=Frag2Rev
     primer_bind     complement(7729..7747)
                     /label=Frag2Reva
     primer_bind     complement(7729..7747)
                     /label=Frag2Rev`b
     primer_bind     join(7769..7773,1..15)
                     /label=TEV_Forward
ORIGIN
        1 ggtgagaact tatactttca gagcggtggc ggctccgtgc caggcgtcgg tgtgcctgga
       61 gtcggggtgc caggggtagg agtccctggc gtaggagtgc caggagtggg tgttccgggt
      121 gtaggagttc ctggagtggg ggttcccggt gttggcgtac cgggcgttgg agtacctggt
      181 gtaggtgtgc ccggtgtagg cgtgcctggc gttggtgttc caggagttgg ggtccccgga
      241 gttggcgttc caggtgtcgg ggttccgggg gttggagtgc ctggtgtggg cgtacctgga
      301 gtaggagtac ccggtgtggg agtgccggga gttggagtcc caggggtcgg cgttcctggg
      361 gtgggagtcc cgggggtggg cgttcccgga gttggggtgc ccggagtagg cgtaccaggc
      421 gtgggagtac cgggtgtggg ggtcccaggt gtaggggtac ccggcgtggg tgtgccgggt
      481 gtcggagttc ccggagtcgg agtccccggg gtgggtgtac cgggggtcgg ggtaccaggg
      541 gtgggggtgc cgggggtagg cgttccgggc gtaggggttc ctggtgtcgg tgttcccggc
      601 gtaggtgttc ctggcgtggg ggtaccggga gtgggcgtcc cgggtgttgg agttccggga
      661 gtaggtgtac ccggagtggg agttccaggc gttggggttc caggggttgg cgtcccagga
      721 gtaggtgtac ctggggtagg tgtccctggt gttggtgtac ctggcgtcgg agtgcccggg
      781 gttggggtgc ctggggttgg tgtgccaggt gttggggtac ctggggtcgg agtaccagga
      841 gtcggtgtac caggtgtggg tgtcccaggc gtaggcgtcc ctggagttgg tgtccccggt
      901 gtcggcgtac ccggggtcgg tgtcccggga gtcggctggc tcgagcacca ccaccaccac
      961 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     1021 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     1081 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     1141 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     1201 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     1261 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     1321 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     1381 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     1441 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     1501 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
     1561 ttacaatttc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1621 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1681 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1741 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1801 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1861 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1921 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     1981 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2041 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2101 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2161 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2221 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     2281 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2341 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2401 gagccggtga gcgtggctct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2461 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2521 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2581 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     2641 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2701 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2761 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2821 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2881 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2941 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3001 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3061 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3121 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3181 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3241 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3301 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3361 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3421 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3481 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     3541 gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     3601 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     3661 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     3721 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     3781 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc
     3841 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata
     3901 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg
     3961 ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg
     4021 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta
     4081 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca
     4141 gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg
     4201 gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt
     4261 catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt
     4321 atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac
     4381 gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggcgataat ggcctgcttc
     4441 tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt
     4501 ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg
     4561 aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc
     4621 ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag
     4681 gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt
     4741 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     4801 atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt
     4861 caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
     4921 caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg
     4981 cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc
     5041 aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc
     5101 aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc
     5161 ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag
     5221 atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa
     5281 cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc
     5341 ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc
     5401 cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt
     5461 aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc
     5521 gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga
     5581 tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc
     5641 aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag
     5701 ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt
     5761 caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt
     5821 tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc
     5881 gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact
     5941 cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga
     6001 atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac
     6061 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga
     6121 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc
     6181 gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga
     6241 attgtgagcg gataacaatt cccctctaga aataattttg tttaacttta agaaggagat
     6301 atacatatgg gtggttgggg accagtgcct ggcgtcggtg tacctggtgc tggtgtaccc
     6361 ggtgctggag taccgggggc aggtgtacca ggtgcagggg tcccaggagc tggagttcca
     6421 ggtgctgggg ttcctggtgc aggtgttcct ggagcaggtg ttcctggtgg cggagttcct
     6481 ggcggtgggg tgcctggtgg gggcgtgcca ggcgggggcg ttcccggtgg cggtgtcccc
     6541 ggtggtgggg ttccaggggg tggggtacca ggcgtcggtg tacctggtgc tggtgtaccc
     6601 ggtgctggag taccgggggc aggtgtacca ggtgcagggg tcccaggagc tggagttcca
     6661 ggtgctgggg ttcctggtgc aggtgttcct ggagcaggtg ttcctggtgg cggagttcct
     6721 ggcggtgggg tgcctggtgg gggcgtgcca ggcgggggcg ttcccggtgg cggtgtcccc
     6781 ggtggtgggg ttccaggggg tggggtccct ggcgtcggtg tacctggtgc tggtgtaccc
     6841 ggtgctggag taccgggggc aggtgtacca ggtgcagggg tcccaggagc tggagttcca
     6901 ggtgctgggg ttcctggtgc aggtgttcct ggagcaggtg ttcctggtgg cggagttcct
     6961 ggcggtgggg tgcctggtgg gggcgtgcca ggcgggggcg ttcccggtgg cggtgtcccc
     7021 ggtggtgggg ttccaggggg tggggtaccg ggagtgggcg tcccaggtgc gggcgttccc
     7081 ggcgcaggtg tgcctggagc gggagtacca ggagccggcg tgccaggggc gggtgtacca
     7141 ggggccggag tgccaggtgc tggagtaccg ggcgctggcg ttcctggtgg tggcgttccg
     7201 ggtggtggag ttcccggtgg tggggttcca ggtgggggag taccaggcgg tggagtccct
     7261 ggcggcggtg taccaggtgg tggtgtgcct ggcgtcggtg tacccggtgc aggggttcca
     7321 ggagcgggag ttcctggtgc gggtgtgccg ggggctggtg tacctggagc aggtgttccg
     7381 ggagctgggg tccctggggc aggagtccca ggcgcaggcg tcccaggggg tggagtgccg
     7441 ggcgggggtg tgccaggtgg cggagtacct gggggcggag taccaggcgg tggtgtgccg
     7501 ggcggcggtg taccgggtgg cggggtaccc ggtgtgggag ttccgggagc tggcgtcccg
     7561 ggtgcaggag tcccaggggc tggagtgcca ggagcaggcg taccaggggc gggagtcccc
     7621 ggagctggtg tccctggtgc cggggtaccg ggtgctggcg taccaggggg cggtgtacca
     7681 ggtggcggcg tacctggtgg tggagttcca ggcggcgggg ttcctggggg tggtgttcca
     7741 ggtgggggtg ttcccggcgg cggttctggt ggc
//
